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The map label for this gene is cobQ

Identifier: 73540371

GI number: 73540371

Start: 713050

End: 714516

Strand: Direct

Name: cobQ

Synonym: Reut_A0665

Alternate gene names: 73540371

Gene position: 713050-714516 (Clockwise)

Preceding gene: 73540366

Following gene: 73540373

Centisome position: 18.73

GC content: 67.69

Gene sequence:

>1467_bases
ATGGTGCAGGGCACGACTTCCGACGCCGGCAAGAGTACGGTCGTGGCAGGCCTGTGCCGCGTACTGGCCCGGGCCGGCAT
GCGCGTGGCGCCATTCAAGCCGCAGAACATGGCGCTGAACAGCGCGGTGACCGCCGATGGTGGCGAGATCGGTCGCGCGC
AGGCGCTGCAGGCACAGGCGGCGCGGGTCGAACCGCACACCGACATGAACCCCGTGCTGCTCAAGCCGAGCAGCGACACC
GGCGCGCAGATCATCATCCACGGCAAAGCCCGGCTGGATCTCGACGCGCGCGCCTATCACGCGTACAAGCCGGAGGCCAT
GAAGGCCGTGCTCGCCTCACATGAACGGCTGACGCAGGCCTACGACGTGGTGGTCGTGGAAGGCGCGGGCAGTCCGGCCG
AGATCAACCTGCGCGATCGCGATATCGCGAACATGGGGTTTGCCGAGCGCGTCGATTGCCCGGTCGTGCTGGTGGCCGAC
ATCGATCGCGGCGGCGTGTTCGCCCATCTGGTGGGCACGCTGGACTGCCTGTCGGACAGTGAGCGCGCACGCGTGACCGG
CTTCATCATCAACCGTTTCCGCGGCGATATCAGCCTGCTGCAGCCGGGGCTCGACTGGCTGGAGGCACGCACCGGCAAGC
CGGTGTTCGGCGTGCTGCCGTACCTGCATGGCCTGCATCTGGATGCGGAAGATGCCATCGTCGGCGCGCAGTCGGCCAAG
CGGGGTACGCCTGACGCGCTGCTGCGCGTGATCGTGCCGGTGTTGCCGCGCATCTCGAACCACACCGATTTCGATGCGCT
GCGCGCGCACCCGCAGGTCGACCTGCGCTTTATCGGGCCCGGCATGCCCGTGCCGCCCGCGGACCTCGTGATCCTGCCCG
GCAGCAAGAGCGTGCGTGCCGACCTGGATTTCCTGCGCGCCAATGGCTGGGAGCCGGCGCTGCGGCGGCATCTGCGTTAT
GGCGGCAAGGTGATCGGCATCTGCGGCGGCATGCAGATGCTGGGCCGGCAGATCCATGATCCGGCCGGCCATGAAGGTCC
TGCTGGCAGCAGCGCAGGGTTCGGCTGGCTCGACTATGAAACGACACTGGCGCCGCACAAGCAGCTGCGCCGAGTCAGCG
GGCGGCTTGCGGATGCTGATCGAGAAGCCGCTGTCAGCGGCTATGAAATCCACATGGGTGTGAGTACCGGCTCAGGCCTG
GAGCGACCGGCACTGTGGCTGGACGATGAAAACGGCACGCGTCGCGCGGATGGTGCCTGCTCAGAGGATGGCCAGGTGCT
GGCGACCTATGTACATGGCGTGTTTGACGAGCCCACGGCATGCGAGGGGCTGCTGCGCTGGGCCGGACTCGACGGGGCAC
AGGGGATCGACCTTGCGGCACTGCGCGAGGCCTCGATCGAACGGCTGGCCGATACACTCGCCAGCCATCTGGACCTTGGG
GCCATGTTTGCGCCGCTGCGCCGCTAG

Upstream 100 bases:

>100_bases
TCCGATAGCCGCACGGCGGGTGCGATAATTGGCCGCTATGCAATGTGATTCTTCTGAAACCTCGCGCACGGACGGTGCCA
GTGCCTTGCGCGGCACGCTG

Downstream 100 bases:

>100_bases
CGCGCGGTGGGTGTCAGGCGCGCGAGCAGTGCGGGCACGTCAGGCCACGTCACGCGCCGTATTGCCACGATGATGTCCGT
GCCCGGAAACGGATCCTGGA

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MVQGTTSDAGKSTVVAGLCRVLARAGMRVAPFKPQNMALNSAVTADGGEIGRAQALQAQAARVEPHTDMNPVLLKPSSDT
GAQIIIHGKARLDLDARAYHAYKPEAMKAVLASHERLTQAYDVVVVEGAGSPAEINLRDRDIANMGFAERVDCPVVLVAD
IDRGGVFAHLVGTLDCLSDSERARVTGFIINRFRGDISLLQPGLDWLEARTGKPVFGVLPYLHGLHLDAEDAIVGAQSAK
RGTPDALLRVIVPVLPRISNHTDFDALRAHPQVDLRFIGPGMPVPPADLVILPGSKSVRADLDFLRANGWEPALRRHLRY
GGKVIGICGGMQMLGRQIHDPAGHEGPAGSSAGFGWLDYETTLAPHKQLRRVSGRLADADREAAVSGYEIHMGVSTGSGL
ERPALWLDDENGTRRADGACSEDGQVLATYVHGVFDEPTACEGLLRWAGLDGAQGIDLAALREASIERLADTLASHLDLG
AMFAPLRR

Sequences:

>Translated_488_residues
MVQGTTSDAGKSTVVAGLCRVLARAGMRVAPFKPQNMALNSAVTADGGEIGRAQALQAQAARVEPHTDMNPVLLKPSSDT
GAQIIIHGKARLDLDARAYHAYKPEAMKAVLASHERLTQAYDVVVVEGAGSPAEINLRDRDIANMGFAERVDCPVVLVAD
IDRGGVFAHLVGTLDCLSDSERARVTGFIINRFRGDISLLQPGLDWLEARTGKPVFGVLPYLHGLHLDAEDAIVGAQSAK
RGTPDALLRVIVPVLPRISNHTDFDALRAHPQVDLRFIGPGMPVPPADLVILPGSKSVRADLDFLRANGWEPALRRHLRY
GGKVIGICGGMQMLGRQIHDPAGHEGPAGSSAGFGWLDYETTLAPHKQLRRVSGRLADADREAAVSGYEIHMGVSTGSGL
ERPALWLDDENGTRRADGACSEDGQVLATYVHGVFDEPTACEGLLRWAGLDGAQGIDLAALREASIERLADTLASHLDLG
AMFAPLRR
>Mature_488_residues
MVQGTTSDAGKSTVVAGLCRVLARAGMRVAPFKPQNMALNSAVTADGGEIGRAQALQAQAARVEPHTDMNPVLLKPSSDT
GAQIIIHGKARLDLDARAYHAYKPEAMKAVLASHERLTQAYDVVVVEGAGSPAEINLRDRDIANMGFAERVDCPVVLVAD
IDRGGVFAHLVGTLDCLSDSERARVTGFIINRFRGDISLLQPGLDWLEARTGKPVFGVLPYLHGLHLDAEDAIVGAQSAK
RGTPDALLRVIVPVLPRISNHTDFDALRAHPQVDLRFIGPGMPVPPADLVILPGSKSVRADLDFLRANGWEPALRRHLRY
GGKVIGICGGMQMLGRQIHDPAGHEGPAGSSAGFGWLDYETTLAPHKQLRRVSGRLADADREAAVSGYEIHMGVSTGSGL
ERPALWLDDENGTRRADGACSEDGQVLATYVHGVFDEPTACEGLLRWAGLDGAQGIDLAALREASIERLADTLASHLDLG
AMFAPLRR

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_CUPPJ (Q474Y6)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294891.1
- ProteinModelPortal:   Q474Y6
- SMR:   Q474Y6
- GeneID:   3610654
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0665
- NMPDR:   fig|264198.3.peg.1154
- HOGENOM:   HBG302271
- OMA:   RGDIANM
- ProtClustDB:   PRK00784
- BioCyc:   REUT264198:REUT_A0665-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 52130; Mature: 52130

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 328-328 ACT_SITE 432-432

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQGTTSDAGKSTVVAGLCRVLARAGMRVAPFKPQNMALNSAVTADGGEIGRAQALQAQA
CCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCEECCEEECCCCCCCHHHHHHHHH
ARVEPHTDMNPVLLKPSSDTGAQIIIHGKARLDLDARAYHAYKPEAMKAVLASHERLTQA
HHCCCCCCCCCEEEECCCCCCCEEEEECCEEECCCHHHHCCCCHHHHHHHHHHHHHHHHE
YDVVVVEGAGSPAEINLRDRDIANMGFAERVDCPVVLVADIDRGGVFAHLVGTLDCLSDS
EEEEEEECCCCCCEEEECCCCHHCCCCHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCC
ERARVTGFIINRFRGDISLLQPGLDWLEARTGKPVFGVLPYLHGLHLDAEDAIVGAQSAK
CCHHHHHHHHHHHCCCHHHHHCCHHHHHHCCCCCHHHHHHHHHCCCCCCHHHEECCCCCC
RGTPDALLRVIVPVLPRISNHTDFDALRAHPQVDLRFIGPGMPVPPADLVILPGSKSVRA
CCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCEEEECCCCCHHH
DLDFLRANGWEPALRRHLRYGGKVIGICGGMQMLGRQIHDPAGHEGPAGSSAGFGWLDYE
HHHHHHCCCCCHHHHHHHHHCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCEEECCC
TTLAPHKQLRRVSGRLADADREAAVSGYEIHMGVSTGSGLERPALWLDDENGTRRADGAC
CCCCHHHHHHHHHCCCCCCCHHHHCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCC
SEDGQVLATYVHGVFDEPTACEGLLRWAGLDGAQGIDLAALREASIERLADTLASHLDLG
CCCCCCCEEEHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHH
AMFAPLRR
HHHHHHCC
>Mature Secondary Structure
MVQGTTSDAGKSTVVAGLCRVLARAGMRVAPFKPQNMALNSAVTADGGEIGRAQALQAQA
CCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCEECCEEECCCCCCCHHHHHHHHH
ARVEPHTDMNPVLLKPSSDTGAQIIIHGKARLDLDARAYHAYKPEAMKAVLASHERLTQA
HHCCCCCCCCCEEEECCCCCCCEEEEECCEEECCCHHHHCCCCHHHHHHHHHHHHHHHHE
YDVVVVEGAGSPAEINLRDRDIANMGFAERVDCPVVLVADIDRGGVFAHLVGTLDCLSDS
EEEEEEECCCCCCEEEECCCCHHCCCCHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCC
ERARVTGFIINRFRGDISLLQPGLDWLEARTGKPVFGVLPYLHGLHLDAEDAIVGAQSAK
CCHHHHHHHHHHHCCCHHHHHCCHHHHHHCCCCCHHHHHHHHHCCCCCCHHHEECCCCCC
RGTPDALLRVIVPVLPRISNHTDFDALRAHPQVDLRFIGPGMPVPPADLVILPGSKSVRA
CCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCEEEECCCCCHHH
DLDFLRANGWEPALRRHLRYGGKVIGICGGMQMLGRQIHDPAGHEGPAGSSAGFGWLDYE
HHHHHHCCCCCHHHHHHHHHCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCEEECCC
TTLAPHKQLRRVSGRLADADREAAVSGYEIHMGVSTGSGLERPALWLDDENGTRRADGAC
CCCCHHHHHHHHHCCCCCCCHHHHCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCC
SEDGQVLATYVHGVFDEPTACEGLLRWAGLDGAQGIDLAALREASIERLADTLASHLDLG
CCCCCCCEEEHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHH
AMFAPLRR
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA