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The map label for this gene is ogt [H]

Identifier: 73540284

GI number: 73540284

Start: 630184

End: 630762

Strand: Reverse

Name: ogt [H]

Synonym: Reut_A0578

Alternate gene names: 73540284

Gene position: 630762-630184 (Counterclockwise)

Preceding gene: 73540285

Following gene: 161611265

Centisome position: 16.57

GC content: 62.52

Gene sequence:

>579_bases
TTGCCTTATCGCGTGAATGGGTTCTTCGGGTCCCTCGCCTCGGTTGCCGTCAATCCCGGTAAAACGATTAAAATCCAGGC
TTCCCTCGATGCTATTGCCATGCCTGCTCCCTTCGACGCCATTCTTCCTGCGCCTTTTGGCAAGGTCGGCGTGCGTGCCG
ACGATGTGCGGATCCGGGAGATTTTTTATCTGCCTGAGGACGTCGAAAGCATTGCGCCGCGCAGTGACGTGATTCTGCGC
CTTGCCGCGCAGCTGGATGCCTATTACGCCGATGCCGGCGCGACCTTTGATGTGCCGCTGGCCATTGCCGGCACGCCGTT
CCAGCAGCAGGTGTGGCAAGCGATCTGCGCGGTGCCGCGCGGCGGCCTGACTACGTATGGCGATATCGCGCGCAAGCTTG
GGGGCGTCGCGCGGGCGGTGGGGCAGGCTTGCGGGCAGAACCCGCTGCCGATCGTGATTCCGTGCCATCGCGTGGTGTCT
GCCAGCGGCATCGGCGGCTTTGCCCACCATGCCGAGGACGGTTTCCATCTGGAAGTGAAACGCTGGCTGTTGCGCCATGA
AGGCGCCATGCTGATATGA

Upstream 100 bases:

>100_bases
AAGATGAAGAATCTGGTGAAGGTGCAGAATATTAAGTTGGAGCAGTAAAAGGCTCTCTTTGCCTTGTACTGGCCGCCTGC
GGGCGGCTTTTTCTTTTGGG

Downstream 100 bases:

>100_bases
AGCGCCCGGCCGGCGCAAGCCAGACCACACGCAAGCCGCGCGCGGTGCATGCCGATGCGGAGATGGTCGCCCTCGATGCG
GCCATCGCCCCCGAACTCGA

Product: methylated-DNA-(protein)-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MPYRVNGFFGSLASVAVNPGKTIKIQASLDAIAMPAPFDAILPAPFGKVGVRADDVRIREIFYLPEDVESIAPRSDVILR
LAAQLDAYYADAGATFDVPLAIAGTPFQQQVWQAICAVPRGGLTTYGDIARKLGGVARAVGQACGQNPLPIVIPCHRVVS
ASGIGGFAHHAEDGFHLEVKRWLLRHEGAMLI

Sequences:

>Translated_192_residues
MPYRVNGFFGSLASVAVNPGKTIKIQASLDAIAMPAPFDAILPAPFGKVGVRADDVRIREIFYLPEDVESIAPRSDVILR
LAAQLDAYYADAGATFDVPLAIAGTPFQQQVWQAICAVPRGGLTTYGDIARKLGGVARAVGQACGQNPLPIVIPCHRVVS
ASGIGGFAHHAEDGFHLEVKRWLLRHEGAMLI
>Mature_191_residues
PYRVNGFFGSLASVAVNPGKTIKIQASLDAIAMPAPFDAILPAPFGKVGVRADDVRIREIFYLPEDVESIAPRSDVILRL
AAQLDAYYADAGATFDVPLAIAGTPFQQQVWQAICAVPRGGLTTYGDIARKLGGVARAVGQACGQNPLPIVIPCHRVVSA
SGIGGFAHHAEDGFHLEVKRWLLRHEGAMLI

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=172, Percent_Identity=33.1395348837209, Blast_Score=67, Evalue=7e-12,
Organism=Escherichia coli, GI1787596, Length=173, Percent_Identity=36.4161849710983, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1788542, Length=75, Percent_Identity=46.6666666666667, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI115533070, Length=90, Percent_Identity=40, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115533068, Length=90, Percent_Identity=40, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320001, Length=63, Percent_Identity=46.031746031746, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20472; Mature: 20341

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYRVNGFFGSLASVAVNPGKTIKIQASLDAIAMPAPFDAILPAPFGKVGVRADDVRIRE
CCEEECHHHHHHHHEEECCCCEEEEEECCCEEECCCCCHHHCCCCCCCCCCCCCCEEEEE
IFYLPEDVESIAPRSDVILRLAAQLDAYYADAGATFDVPLAIAGTPFQQQVWQAICAVPR
EEECCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEECEEEEECCCHHHHHHHHHHCCCC
GGLTTYGDIARKLGGVARAVGQACGQNPLPIVIPCHRVVSASGIGGFAHHAEDGFHLEVK
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCCHHHCCCCCCCHHHH
RWLLRHEGAMLI
HHHHHCCCCCCC
>Mature Secondary Structure 
PYRVNGFFGSLASVAVNPGKTIKIQASLDAIAMPAPFDAILPAPFGKVGVRADDVRIRE
CEEECHHHHHHHHEEECCCCEEEEEECCCEEECCCCCHHHCCCCCCCCCCCCCCEEEEE
IFYLPEDVESIAPRSDVILRLAAQLDAYYADAGATFDVPLAIAGTPFQQQVWQAICAVPR
EEECCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEECEEEEECCCHHHHHHHHHHCCCC
GGLTTYGDIARKLGGVARAVGQACGQNPLPIVIPCHRVVSASGIGGFAHHAEDGFHLEVK
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCCHHHCCCCCCCHHHH
RWLLRHEGAMLI
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]