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The map label for this gene is oprM [H]

Identifier: 73540275

GI number: 73540275

Start: 616113

End: 617573

Strand: Reverse

Name: oprM [H]

Synonym: Reut_A0569

Alternate gene names: 73540275

Gene position: 617573-616113 (Counterclockwise)

Preceding gene: 73540276

Following gene: 73540274

Centisome position: 16.22

GC content: 67.28

Gene sequence:

>1461_bases
ATGATGAAGATGAATCCGCTTTCCCGTTTCCTCTCCCTTGGCGCCGTGCCGCTGGCCTGCGCGCTCCTGCTCGCCGGCTG
CGCCGTCGGCCCCGATTACCAGCGGCCCGACGCGCCGACATCGCCCGCTTTCAAGGAAGCCCCCGCCGACGCCGAAGGGT
GGACCGGCGAGTGGAAGACCGCCGAGCCGCAGGACGCGCACGCCCGCCCACAATGGTGGCAGGTATTCGGCGACAGCCAG
CTCGACGGGCTGATGTCGCAGGTACAGATCTCGAACCAGAACATCAAGGCCGCCGAGGCGCAGTACCGGCAGGCGCAGGC
ATCGCTGCAGGCAGCGCGGGCCGGCTTCTTCCCAACCGTCGATGCCCAGGCCGGCGTGTCGCGCGCGCGCGGCGCAAGCG
GCAATACGATCAGCGGCCAAAGCGTCACACTCGGCGCCACGTGGGAACTCGACGTCTGGGGTCGCGTACGTCGCCAGGTG
GAAAGCAGCGAAGCGAACGCGCAGGCAAGCCAGGCAGACCTTGCCTCGACGCTGCTGTCGACGCAGGCAACGCTCGCGCA
AAGCTATTTCCTGCTGCGCATCGCCGACGCGCAAAAAGCGCTACTGGACCGCACGGTGGCGGACTACGCGAAATCGCTGC
AGCTGGTCCAGAACCAGTACAAGGCCGGCACGGCGCAAAGGTCTGACGTGCTGCAGTCCGAAACGCAGCTCAAGAGCGCG
CAGGCGCAGCAGCTCGATATCCAGATCACGCGCGCGCAGCTCGAACATGCGATCGCCGTGCTGATCGGCAAGCCGCCTGC
GGAACTGTCGGTCGGGCAGGCTGATTTCGCTTCCACGCCGCCGCGTGTGCCGGTGGCCGTGCCGTCGCAGCTTCTGGAAC
GGCGGCCGGACATTGGTGCGGCCGAGCGTCGGATGGCGTCCGCGAATGCGCAGATCGGCGTGGCACAGGCTGCGTACTAT
CCGACGCTGTCGCTGTCCGCCAGCGGCGGGCTGACTGCGAGCACGCTTGCGCGCTGGGTGTCGCTGCCCGACAGGATCTG
GTCGGTGGGTGCCGGGCTGGCTGGGACGCTGTTTGATGGTGGGCTGCGCAGTGCTGCGAAGGCGCAGGCGGTGGCGGCCT
ATGACCAGACCGTGGCCAACTATCGGCAGACGGTGTTGGGGGCGTTCCAGGAGGTTGAGGACAACCTTGCTGCGCAGCGG
CTGCTGGAGCAGGAATCGGTTGTGCAGAATGATGCGGTACGGGCGGCGCGGGAGGCGTTAGCGCTCGTCAATAACCGGTA
TCGCGCCGGCACCGCCAGCTTGCTTGATGTGCTGGTCGCCCAGACGACGGCTTATACGGCGGAGCGGACTGCGTTGACCC
TGATGGGACGCCAATACACCGCTAGCGTGGTGCTGATCAAGGCGCTCGGCGGGGGGTGGCATCAGGGGGATGGTGGGGTG
GAGACTGTGGGACAGCGGTGA

Upstream 100 bases:

>100_bases
ACCTGACGCTGGATCGCTGGCGCCTGAAAGTGAAGGCCTGGCGCGAACGCCGCCGCGGCAACCCGCACGGCTCCGCACCG
GCTGCCGGCACCGAATCCTG

Downstream 100 bases:

>100_bases
TTTGCGTGGTTATCGTTCTTGGCTAGTGCCGCTGTTTCGCCGGCGTAGCCGGCGACTTACGGGCCTGTCAGAAATTTTGT
GTTTAGGGCATAACATGTCA

Product: RND efflux system, outer membrane lipoprotein, NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MMKMNPLSRFLSLGAVPLACALLLAGCAVGPDYQRPDAPTSPAFKEAPADAEGWTGEWKTAEPQDAHARPQWWQVFGDSQ
LDGLMSQVQISNQNIKAAEAQYRQAQASLQAARAGFFPTVDAQAGVSRARGASGNTISGQSVTLGATWELDVWGRVRRQV
ESSEANAQASQADLASTLLSTQATLAQSYFLLRIADAQKALLDRTVADYAKSLQLVQNQYKAGTAQRSDVLQSETQLKSA
QAQQLDIQITRAQLEHAIAVLIGKPPAELSVGQADFASTPPRVPVAVPSQLLERRPDIGAAERRMASANAQIGVAQAAYY
PTLSLSASGGLTASTLARWVSLPDRIWSVGAGLAGTLFDGGLRSAAKAQAVAAYDQTVANYRQTVLGAFQEVEDNLAAQR
LLEQESVVQNDAVRAAREALALVNNRYRAGTASLLDVLVAQTTAYTAERTALTLMGRQYTASVVLIKALGGGWHQGDGGV
ETVGQR

Sequences:

>Translated_486_residues
MMKMNPLSRFLSLGAVPLACALLLAGCAVGPDYQRPDAPTSPAFKEAPADAEGWTGEWKTAEPQDAHARPQWWQVFGDSQ
LDGLMSQVQISNQNIKAAEAQYRQAQASLQAARAGFFPTVDAQAGVSRARGASGNTISGQSVTLGATWELDVWGRVRRQV
ESSEANAQASQADLASTLLSTQATLAQSYFLLRIADAQKALLDRTVADYAKSLQLVQNQYKAGTAQRSDVLQSETQLKSA
QAQQLDIQITRAQLEHAIAVLIGKPPAELSVGQADFASTPPRVPVAVPSQLLERRPDIGAAERRMASANAQIGVAQAAYY
PTLSLSASGGLTASTLARWVSLPDRIWSVGAGLAGTLFDGGLRSAAKAQAVAAYDQTVANYRQTVLGAFQEVEDNLAAQR
LLEQESVVQNDAVRAAREALALVNNRYRAGTASLLDVLVAQTTAYTAERTALTLMGRQYTASVVLIKALGGGWHQGDGGV
ETVGQR
>Mature_486_residues
MMKMNPLSRFLSLGAVPLACALLLAGCAVGPDYQRPDAPTSPAFKEAPADAEGWTGEWKTAEPQDAHARPQWWQVFGDSQ
LDGLMSQVQISNQNIKAAEAQYRQAQASLQAARAGFFPTVDAQAGVSRARGASGNTISGQSVTLGATWELDVWGRVRRQV
ESSEANAQASQADLASTLLSTQATLAQSYFLLRIADAQKALLDRTVADYAKSLQLVQNQYKAGTAQRSDVLQSETQLKSA
QAQQLDIQITRAQLEHAIAVLIGKPPAELSVGQADFASTPPRVPVAVPSQLLERRPDIGAAERRMASANAQIGVAQAAYY
PTLSLSASGGLTASTLARWVSLPDRIWSVGAGLAGTLFDGGLRSAAKAQAVAAYDQTVANYRQTVLGAFQEVEDNLAAQR
LLEQESVVQNDAVRAAREALALVNNRYRAGTASLLDVLVAQTTAYTAERTALTLMGRQYTASVVLIKALGGGWHQGDGGV
ETVGQR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=462, Percent_Identity=27.2727272727273, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI2367348, Length=497, Percent_Identity=26.1569416498994, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51645; Mature: 51645

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKMNPLSRFLSLGAVPLACALLLAGCAVGPDYQRPDAPTSPAFKEAPADAEGWTGEWKT
CCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCC
AEPQDAHARPQWWQVFGDSQLDGLMSQVQISNQNIKAAEAQYRQAQASLQAARAGFFPTV
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
DAQAGVSRARGASGNTISGQSVTLGATWELDVWGRVRRQVESSEANAQASQADLASTLLS
CHHHHHHHHCCCCCCCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQATLAQSYFLLRIADAQKALLDRTVADYAKSLQLVQNQYKAGTAQRSDVLQSETQLKSA
HHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
QAQQLDIQITRAQLEHAIAVLIGKPPAELSVGQADFASTPPRVPVAVPSQLLERRPDIGA
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCH
AERRMASANAQIGVAQAAYYPTLSLSASGGLTASTLARWVSLPDRIWSVGAGLAGTLFDG
HHHHHHHCCCCEEEHHHHHCCEEEECCCCCCCHHHHHHHHHCHHHHHHHCCCHHHHHHHH
GLRSAAKAQAVAAYDQTVANYRQTVLGAFQEVEDNLAAQRLLEQESVVQNDAVRAAREAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVNNRYRAGTASLLDVLVAQTTAYTAERTALTLMGRQYTASVVLIKALGGGWHQGDGGV
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEHHCCCCCCCCCCH
ETVGQR
HHCCCC
>Mature Secondary Structure
MMKMNPLSRFLSLGAVPLACALLLAGCAVGPDYQRPDAPTSPAFKEAPADAEGWTGEWKT
CCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCC
AEPQDAHARPQWWQVFGDSQLDGLMSQVQISNQNIKAAEAQYRQAQASLQAARAGFFPTV
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
DAQAGVSRARGASGNTISGQSVTLGATWELDVWGRVRRQVESSEANAQASQADLASTLLS
CHHHHHHHHCCCCCCCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQATLAQSYFLLRIADAQKALLDRTVADYAKSLQLVQNQYKAGTAQRSDVLQSETQLKSA
HHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
QAQQLDIQITRAQLEHAIAVLIGKPPAELSVGQADFASTPPRVPVAVPSQLLERRPDIGA
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCH
AERRMASANAQIGVAQAAYYPTLSLSASGGLTASTLARWVSLPDRIWSVGAGLAGTLFDG
HHHHHHHCCCCEEEHHHHHCCEEEECCCCCCCHHHHHHHHHCHHHHHHHCCCHHHHHHHH
GLRSAAKAQAVAAYDQTVANYRQTVLGAFQEVEDNLAAQRLLEQESVVQNDAVRAAREAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVNNRYRAGTASLLDVLVAQTTAYTAERTALTLMGRQYTASVVLIKALGGGWHQGDGGV
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEHHCCCCCCCCCCH
ETVGQR
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]