Click here to switch to the map view.

The map label for this gene is rfaF [H]

Identifier: 73540251

GI number: 73540251

Start: 591012

End: 592034

Strand: Reverse

Name: rfaF [H]

Synonym: Reut_A0545

Alternate gene names: 73540251

Gene position: 592034-591012 (Counterclockwise)

Preceding gene: 73540252

Following gene: 73540250

Centisome position: 15.55

GC content: 67.84

Gene sequence:

>1023_bases
ATGAAAAAAGCCCTCGTCATCGCCCCTGACCGGATCAGCGACGCCCTGATGGCCCAGCCGCTGCTCGGGCTGCTCAAGGC
GCGCCATCCGCGGCTGGTCATCGACGCCCTTGCACCCAAATGGGTCGGCCCGGTGCTGGCGCGCATGCCGGAGATGGGCA
GGGTCTTCCCGAGCGACCTGACGCACGGGAAGCTGCAGCTTTCGGCACGGTTCATGTTCGCCCAGCAGCTCAAGAACGAA
GGCTACGACATGGCCTACGTGCTGCCGGATTCGCTCAAGTCAGCGCTGATTCCGTGGCTGGCAGGCATTCCGCTGCGCGT
CGGCTACCGCGGCGAGTCGCGCTTCGGCATGCTGAACGTGCGCCATGCCAACCCGCCGCGCAACGGCCGCCCGGTCATGG
CCGAGCATTACGCGCGCCTGGCGCTGCGGCCCGGCGCAAAGCCGCCGGCGGAATTGCCCGAACCGCGACTGCGCACCGAT
GCCGTCCGGATCGCCGCCACGGCCGAGCGCTTCGGTATCCCGCCGCAGACGCGCGTGATCGCGTTCTGCCCGGGCGCCGG
CTTCGGCCCCGCCCGTCGCTGGCCTGCCGCGCATTTCGCGAAGCTCGCACAGATGCTGAGGCGCTCGTACCCGTATGCCC
AGATGATCACGCTGGGCGCGGCCGCCGATGCGGACGCCGCTGCGGAGATCACGAGCGAAGCGCCGTTCGTGCGCAACCTG
TGCGGCAAGACTTCGCTCGACGATGCCATCGACCTGCTCGCGCTGTCCGAAGCGGCCGTATGCAACGACACCGGCCTCAT
GCACATCGCCGCGGCGCTGAACCGCCCGCAAGTTGCGGTGTTCGGCTCGAGCGATCCGCGCCAGACGCCGCCCCTGTCAC
AGGCAGCGAGTATCATGTGGCTGCAACTCGAATGCAGTCCGTGCCTCAAACACGAGTGTCCCCTGGGACACATGCGCTGC
CTGAAGGACATCGAGCCCGAAATGGTGTTCGTTGAACTGCGCAAGCTGCTGCACCGGCCCTGA

Upstream 100 bases:

>100_bases
CACGCTCCTGCAGCACTCCGACGCTCCGGGCCGCCTGGCGGCGCCCCGGGGCGTTGGCATTTCCGCCCGCCACTGACGGC
GGGCAAGCTTCGCCCCACTC

Downstream 100 bases:

>100_bases
CCCCTGAAATCGCGACCATGCCCCGTTTTGCCCGTCTGTTCGATTCCGCGGAAGACATTGTCCAGGCCTTCCGCGAGGCC
ATGAAACTGCGCGATGCCGA

Product: lipopolysaccharide heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKKALVIAPDRISDALMAQPLLGLLKARHPRLVIDALAPKWVGPVLARMPEMGRVFPSDLTHGKLQLSARFMFAQQLKNE
GYDMAYVLPDSLKSALIPWLAGIPLRVGYRGESRFGMLNVRHANPPRNGRPVMAEHYARLALRPGAKPPAELPEPRLRTD
AVRIAATAERFGIPPQTRVIAFCPGAGFGPARRWPAAHFAKLAQMLRRSYPYAQMITLGAAADADAAAEITSEAPFVRNL
CGKTSLDDAIDLLALSEAAVCNDTGLMHIAAALNRPQVAVFGSSDPRQTPPLSQAASIMWLQLECSPCLKHECPLGHMRC
LKDIEPEMVFVELRKLLHRP

Sequences:

>Translated_340_residues
MKKALVIAPDRISDALMAQPLLGLLKARHPRLVIDALAPKWVGPVLARMPEMGRVFPSDLTHGKLQLSARFMFAQQLKNE
GYDMAYVLPDSLKSALIPWLAGIPLRVGYRGESRFGMLNVRHANPPRNGRPVMAEHYARLALRPGAKPPAELPEPRLRTD
AVRIAATAERFGIPPQTRVIAFCPGAGFGPARRWPAAHFAKLAQMLRRSYPYAQMITLGAAADADAAAEITSEAPFVRNL
CGKTSLDDAIDLLALSEAAVCNDTGLMHIAAALNRPQVAVFGSSDPRQTPPLSQAASIMWLQLECSPCLKHECPLGHMRC
LKDIEPEMVFVELRKLLHRP
>Mature_340_residues
MKKALVIAPDRISDALMAQPLLGLLKARHPRLVIDALAPKWVGPVLARMPEMGRVFPSDLTHGKLQLSARFMFAQQLKNE
GYDMAYVLPDSLKSALIPWLAGIPLRVGYRGESRFGMLNVRHANPPRNGRPVMAEHYARLALRPGAKPPAELPEPRLRTD
AVRIAATAERFGIPPQTRVIAFCPGAGFGPARRWPAAHFAKLAQMLRRSYPYAQMITLGAAADADAAAEITSEAPFVRNL
CGKTSLDDAIDLLALSEAAVCNDTGLMHIAAALNRPQVAVFGSSDPRQTPPLSQAASIMWLQLECSPCLKHECPLGHMRC
LKDIEPEMVFVELRKLLHRP

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=347, Percent_Identity=42.3631123919308, Blast_Score=260, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 37288; Mature: 37288

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALVIAPDRISDALMAQPLLGLLKARHPRLVIDALAPKWVGPVLARMPEMGRVFPSDL
CCCEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEHHCCHHHHHHHHHCCHHCCCCCCCC
THGKLQLSARFMFAQQLKNEGYDMAYVLPDSLKSALIPWLAGIPLRVGYRGESRFGMLNV
CCCEEEHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCEEEECCCCCCEEEEEE
RHANPPRNGRPVMAEHYARLALRPGAKPPAELPEPRLRTDAVRIAATAERFGIPPQTRVI
EECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHEEHHHHHCCCCCCCEE
AFCPGAGFGPARRWPAAHFAKLAQMLRRSYPYAQMITLGAAADADAAAEITSEAPFVRNL
EECCCCCCCCHHCCCHHHHHHHHHHHHHCCCHHHHHEECCCCCCCHHHHHHCCCHHHHHH
CGKTSLDDAIDLLALSEAAVCNDTGLMHIAAALNRPQVAVFGSSDPRQTPPLSQAASIMW
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCHHHHCEEEE
LQLECSPCLKHECPLGHMRCLKDIEPEMVFVELRKLLHRP
EEEECCHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKALVIAPDRISDALMAQPLLGLLKARHPRLVIDALAPKWVGPVLARMPEMGRVFPSDL
CCCEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEHHCCHHHHHHHHHCCHHCCCCCCCC
THGKLQLSARFMFAQQLKNEGYDMAYVLPDSLKSALIPWLAGIPLRVGYRGESRFGMLNV
CCCEEEHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCEEEECCCCCCEEEEEE
RHANPPRNGRPVMAEHYARLALRPGAKPPAELPEPRLRTDAVRIAATAERFGIPPQTRVI
EECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHEEHHHHHCCCCCCCEE
AFCPGAGFGPARRWPAAHFAKLAQMLRRSYPYAQMITLGAAADADAAAEITSEAPFVRNL
EECCCCCCCCHHCCCHHHHHHHHHHHHHCCCHHHHHEECCCCCCCHHHHHHCCCHHHHHH
CGKTSLDDAIDLLALSEAAVCNDTGLMHIAAALNRPQVAVFGSSDPRQTPPLSQAASIMW
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCHHHHCEEEE
LQLECSPCLKHECPLGHMRCLKDIEPEMVFVELRKLLHRP
EEEECCHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]