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The map label for this gene is ynfJ [C]

Identifier: 73540222

GI number: 73540222

Start: 560632

End: 562437

Strand: Reverse

Name: ynfJ [C]

Synonym: Reut_A0516

Alternate gene names: 73540222

Gene position: 562437-560632 (Counterclockwise)

Preceding gene: 73540225

Following gene: 73540221

Centisome position: 14.78

GC content: 69.71

Gene sequence:

>1806_bases
ATGCCGGTTGCTACACACGCTACGTTGCGGGAGTGTGGCCCTGCGCTATCATGCGGCCACCATCTGCCCCCGAACGCTGA
AAGCCGCGCCGCCGCGCGGCCGGCGCCAGCACCCATGCGGTTCCGATTTCTCGAAGACCAGGAAGTCACGCTGTTTGCGG
CGATTCCGGTCGGCATTCTTGCTGCGATTGCGACCACCCTCTTCAAGGAAGCGCTGGAGGGGGCCGGCATCGTCATCTTT
TCCAGCAACGCGGATATCGTCGCGATCTTTGCGGCGCTGGCGCTGGCATGGCGCATCGTGGTGCCGGCGGTCGGAGGCGC
GGTGGCCGGGCTGCTGCTACAAGCCGCTAACCGGCTCGCTGGCAGCGGCGGCGCGACCGACTACATGGAGGCCGTAGCCA
ACGGCACGGGCCGCCTGCCCGTGCGCGTTACGCTGCTGCGGTCGCTATCGTCGTTTTGCTCCATCGTCAGCGGGGCTTCG
GTCGGCAAGGAAGGCGCCATGATCCAGCTCGCTGCGTTGTGCGGCGCGCTGTTTCCATCCACGCGCATCGGACAGCCCCA
CGGCGCTTCGAACATGCGGCGCATGCTGACTGCGTGTGGCGCTGCCGCTGGCCTGGCCACGGTCTACCACACGCCGCTGT
CCGCGGCCGTGTTCGTCGCCGAAGTGGTGTTCGGAGCACTGGCCGTGCAGCGCCTGATGCCGCTGTTCCTGGCCTCGGTG
GCGGGCGCCATGGTCAGCCAGTGGCATGGCGGCCTGCAGCCGCTGTATCCCGGCCTGCACATCGCAGCCGACGTGCGTCC
GCAATTGATGCTGGCCGCGGCGGCGCTGGGGGTGGCCGCCGGCATCGTCGGGGCATTGTTCCTGCGCGCCGCCAGCATCG
CGCGCGGGCGCTTTGCGCAGGTGCCCGGCGGCCCGGTCGCGCGGCTCGCGCTCGGCGGCGCACTGGTCGGCGCACTGGCC
ATGGCCGTGCCCGAAGTGGTCGGCAACGGCTTTTCCACGATCCAGACCATCCTGCAGGAACAGCCGCTGACAGTGTCGGT
CGGCCTGGTGCTGCTGGCCAAGCTCGCGGCGACGGTCATCAGCATGGGCTCGGGCGCCGTCGGCGGTGTGTTCACGCCTT
CGCTGTTCGTCGGCGCCGCGCTGGGCCAGTTGCTGGCGCTCGCAATGCAGGCGTTCGTGCCCGGCGCGCCGGCCTCGCCG
GTCCTGCCGCTGGTCGGCATGAGCGCGTTCCTTGCCGCGACCAGCCAGGCGCCGCTGATGTCGGTGCTGATGGTGTTCGA
AATGACGCTGGCGCCGGCTCTGCTGTTGCCCACCATGATCGGCGCGGTGGCGGCGTATTACACGGCGTCGCGCTGCCAGA
CGCTCTCGCTCTACAGCGTGATCGCCGACCGCGCGCAGGCGTCGGCCGCGGAAGAACATGCGCGGGCAATGACGCTGGCC
GGCCTGTGCGACCCGACCGATACCACGCTCGCGCCGGATGCCTCGCTCGCCGCTGCCGCCGACAAGTTTGCGGAGACCGG
CACACGCTACCTGTACCTGGTCGAGCCCGACGGGCGCCTGCTCGGGGCGCTGTCAATCCACGCGCTGCAACGCGCGCAGC
GCGAAGACTCGGCCGCCGGCGTGCTCGCGCTAGCCGAACGCGACTTCCCGGCGCTGACGCCTGAGTCGCGGCTGCGCGAC
GCCCTGGAGGTCTTCGCCGCCCATGGCATCAACCGCATTCCGCTCGTGCGCGATGGCGCCAGCCGCGAACTGATGGGCAC
CGTTTCCAAGCAGCGTGTGCTGCAGGAAGCATCCTGTCTGTTCTAG

Upstream 100 bases:

>100_bases
CCAGTCGGGGGACCGGCATAGCGGCGCGAGGCGTCCGCTGACGAAACAAAGGTCGGTAGTGTAACAGCCAAGCGCAAACC
TATTGATCACATGGTGATGC

Downstream 100 bases:

>100_bases
GCGACGGGGCACCCGTGCCTCGAGCGATGCCGGACACGGTGCCATCGAACGGGTAAAATACCCGGTTACCCTTCAGCCTC
CTTTTCAACCTGATCTGCGC

Product: putative voltage-gated ClC-type chloride channel ClcB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 601; Mature: 600

Protein sequence:

>601_residues
MPVATHATLRECGPALSCGHHLPPNAESRAAARPAPAPMRFRFLEDQEVTLFAAIPVGILAAIATTLFKEALEGAGIVIF
SSNADIVAIFAALALAWRIVVPAVGGAVAGLLLQAANRLAGSGGATDYMEAVANGTGRLPVRVTLLRSLSSFCSIVSGAS
VGKEGAMIQLAALCGALFPSTRIGQPHGASNMRRMLTACGAAAGLATVYHTPLSAAVFVAEVVFGALAVQRLMPLFLASV
AGAMVSQWHGGLQPLYPGLHIAADVRPQLMLAAAALGVAAGIVGALFLRAASIARGRFAQVPGGPVARLALGGALVGALA
MAVPEVVGNGFSTIQTILQEQPLTVSVGLVLLAKLAATVISMGSGAVGGVFTPSLFVGAALGQLLALAMQAFVPGAPASP
VLPLVGMSAFLAATSQAPLMSVLMVFEMTLAPALLLPTMIGAVAAYYTASRCQTLSLYSVIADRAQASAAEEHARAMTLA
GLCDPTDTTLAPDASLAAAADKFAETGTRYLYLVEPDGRLLGALSIHALQRAQREDSAAGVLALAERDFPALTPESRLRD
ALEVFAAHGINRIPLVRDGASRELMGTVSKQRVLQEASCLF

Sequences:

>Translated_601_residues
MPVATHATLRECGPALSCGHHLPPNAESRAAARPAPAPMRFRFLEDQEVTLFAAIPVGILAAIATTLFKEALEGAGIVIF
SSNADIVAIFAALALAWRIVVPAVGGAVAGLLLQAANRLAGSGGATDYMEAVANGTGRLPVRVTLLRSLSSFCSIVSGAS
VGKEGAMIQLAALCGALFPSTRIGQPHGASNMRRMLTACGAAAGLATVYHTPLSAAVFVAEVVFGALAVQRLMPLFLASV
AGAMVSQWHGGLQPLYPGLHIAADVRPQLMLAAAALGVAAGIVGALFLRAASIARGRFAQVPGGPVARLALGGALVGALA
MAVPEVVGNGFSTIQTILQEQPLTVSVGLVLLAKLAATVISMGSGAVGGVFTPSLFVGAALGQLLALAMQAFVPGAPASP
VLPLVGMSAFLAATSQAPLMSVLMVFEMTLAPALLLPTMIGAVAAYYTASRCQTLSLYSVIADRAQASAAEEHARAMTLA
GLCDPTDTTLAPDASLAAAADKFAETGTRYLYLVEPDGRLLGALSIHALQRAQREDSAAGVLALAERDFPALTPESRLRD
ALEVFAAHGINRIPLVRDGASRELMGTVSKQRVLQEASCLF
>Mature_600_residues
PVATHATLRECGPALSCGHHLPPNAESRAAARPAPAPMRFRFLEDQEVTLFAAIPVGILAAIATTLFKEALEGAGIVIFS
SNADIVAIFAALALAWRIVVPAVGGAVAGLLLQAANRLAGSGGATDYMEAVANGTGRLPVRVTLLRSLSSFCSIVSGASV
GKEGAMIQLAALCGALFPSTRIGQPHGASNMRRMLTACGAAAGLATVYHTPLSAAVFVAEVVFGALAVQRLMPLFLASVA
GAMVSQWHGGLQPLYPGLHIAADVRPQLMLAAAALGVAAGIVGALFLRAASIARGRFAQVPGGPVARLALGGALVGALAM
AVPEVVGNGFSTIQTILQEQPLTVSVGLVLLAKLAATVISMGSGAVGGVFTPSLFVGAALGQLLALAMQAFVPGAPASPV
LPLVGMSAFLAATSQAPLMSVLMVFEMTLAPALLLPTMIGAVAAYYTASRCQTLSLYSVIADRAQASAAEEHARAMTLAG
LCDPTDTTLAPDASLAAAADKFAETGTRYLYLVEPDGRLLGALSIHALQRAQREDSAAGVLALAERDFPALTPESRLRDA
LEVFAAHGINRIPLVRDGASRELMGTVSKQRVLQEASCLF

Specific function: Unknown

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081943, Length=405, Percent_Identity=37.5308641975309, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI1786350, Length=207, Percent_Identity=31.4009661835749, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17532615, Length=369, Percent_Identity=23.0352303523035, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 61398; Mature: 61266

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVATHATLRECGPALSCGHHLPPNAESRAAARPAPAPMRFRFLEDQEVTLFAAIPVGIL
CCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEHHHHHHH
AAIATTLFKEALEGAGIVIFSSNADIVAIFAALALAWRIVVPAVGGAVAGLLLQAANRLA
HHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSGGATDYMEAVANGTGRLPVRVTLLRSLSSFCSIVSGASVGKEGAMIQLAALCGALFPS
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCC
TRIGQPHGASNMRRMLTACGAAAGLATVYHTPLSAAVFVAEVVFGALAVQRLMPLFLASV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGAMVSQWHGGLQPLYPGLHIAADVRPQLMLAAAALGVAAGIVGALFLRAASIARGRFAQ
HHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VPGGPVARLALGGALVGALAMAVPEVVGNGFSTIQTILQEQPLTVSVGLVLLAKLAATVI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHH
SMGSGAVGGVFTPSLFVGAALGQLLALAMQAFVPGAPASPVLPLVGMSAFLAATSQAPLM
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHH
SVLMVFEMTLAPALLLPTMIGAVAAYYTASRCQTLSLYSVIADRAQASAAEEHARAMTLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLCDPTDTTLAPDASLAAAADKFAETGTRYLYLVEPDGRLLGALSIHALQRAQREDSAAG
HCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHHHHCCCCCC
VLALAERDFPALTPESRLRDALEVFAAHGINRIPLVRDGASRELMGTVSKQRVLQEASCL
EEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCC
F
C
>Mature Secondary Structure 
PVATHATLRECGPALSCGHHLPPNAESRAAARPAPAPMRFRFLEDQEVTLFAAIPVGIL
CCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEHHHHHHH
AAIATTLFKEALEGAGIVIFSSNADIVAIFAALALAWRIVVPAVGGAVAGLLLQAANRLA
HHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSGGATDYMEAVANGTGRLPVRVTLLRSLSSFCSIVSGASVGKEGAMIQLAALCGALFPS
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCC
TRIGQPHGASNMRRMLTACGAAAGLATVYHTPLSAAVFVAEVVFGALAVQRLMPLFLASV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGAMVSQWHGGLQPLYPGLHIAADVRPQLMLAAAALGVAAGIVGALFLRAASIARGRFAQ
HHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VPGGPVARLALGGALVGALAMAVPEVVGNGFSTIQTILQEQPLTVSVGLVLLAKLAATVI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHH
SMGSGAVGGVFTPSLFVGAALGQLLALAMQAFVPGAPASPVLPLVGMSAFLAATSQAPLM
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHH
SVLMVFEMTLAPALLLPTMIGAVAAYYTASRCQTLSLYSVIADRAQASAAEEHARAMTLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLCDPTDTTLAPDASLAAAADKFAETGTRYLYLVEPDGRLLGALSIHALQRAQREDSAAG
HCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHHHHCCCCCC
VLALAERDFPALTPESRLRDALEVFAAHGINRIPLVRDGASRELMGTVSKQRVLQEASCL
EEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]