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The map label for this gene is gloA [H]

Identifier: 73540209

GI number: 73540209

Start: 547229

End: 547636

Strand: Reverse

Name: gloA [H]

Synonym: Reut_A0503

Alternate gene names: 73540209

Gene position: 547636-547229 (Counterclockwise)

Preceding gene: 73540210

Following gene: 73540208

Centisome position: 14.39

GC content: 65.69

Gene sequence:

>408_bases
ATGCGACTCCTCCACACCATGCTGCGCGTCGGCGACCTGCAGCGCTCCATCGACTTCTACACCCGCGTGCTCGGCATGCA
GCTGCTGCGCCAGAGCGACAACCCGGAATACAAGTACCGCCTGGCCTTCGTCGGCTATGGCCCGGAAAGCGAGACCGCGG
TGCTGGAACTGACCTACAACTACGGCGTCGACAAGTATGACCTCGGTACCGCCTACGGCCACATCGCGCTGGAAACCGAC
AATGCCGCGGCGGCCTGCGACCGCATCCGCGTGGCGGGCGGCAAGGTCGCGCGTGAAGCCGGTCCGGTCAAGGGCGGCAC
GACGGTAATCGCCTTCGTCGAAGACCCGGACGGCTACAAGATCGAACTGATCGAGCGCCATTCCACGCGCGACGCCAGCC
GTCCCTGA

Upstream 100 bases:

>100_bases
GGCAGCGCGGCCGCGCTGCCGGCCCGCCGGTGCGTTTGGCACAAACGCACTAAAATGACGCACTCCCTCCAAGAACGACC
CGACTCATAGGGCATCTCAA

Downstream 100 bases:

>100_bases
GCGCGCCCGGCATGGACGCGCAAAGCCTGAGCCGGCGCCCGCTCGATGGCGCCGCCATCGGCCTGATGGTGGTGCTGTGC
GCCGCCTGGGGACTGCAACA

Product: glyoxalase I

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MRLLHTMLRVGDLQRSIDFYTRVLGMQLLRQSDNPEYKYRLAFVGYGPESETAVLELTYNYGVDKYDLGTAYGHIALETD
NAAAACDRIRVAGGKVAREAGPVKGGTTVIAFVEDPDGYKIELIERHSTRDASRP

Sequences:

>Translated_135_residues
MRLLHTMLRVGDLQRSIDFYTRVLGMQLLRQSDNPEYKYRLAFVGYGPESETAVLELTYNYGVDKYDLGTAYGHIALETD
NAAAACDRIRVAGGKVAREAGPVKGGTTVIAFVEDPDGYKIELIERHSTRDASRP
>Mature_135_residues
MRLLHTMLRVGDLQRSIDFYTRVLGMQLLRQSDNPEYKYRLAFVGYGPESETAVLELTYNYGVDKYDLGTAYGHIALETD
NAAAACDRIRVAGGKVAREAGPVKGGTTVIAFVEDPDGYKIELIERHSTRDASRP

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=146, Percent_Identity=36.3013698630137, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1787940, Length=134, Percent_Identity=81.3432835820896, Blast_Score=226, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI17552228, Length=133, Percent_Identity=32.3308270676692, Blast_Score=72, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=39.568345323741, Blast_Score=99, Evalue=3e-22,
Organism=Drosophila melanogaster, GI20129743, Length=148, Percent_Identity=35.8108108108108, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19920404, Length=138, Percent_Identity=31.1594202898551, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 15029; Mature: 15029

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00934 GLYOXALASE_I_1 ; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLHTMLRVGDLQRSIDFYTRVLGMQLLRQSDNPEYKYRLAFVGYGPESETAVLELTYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCEEEEEEEEC
YGVDKYDLGTAYGHIALETDNAAAACDRIRVAGGKVAREAGPVKGGTTVIAFVEDPDGYK
CCCCCEECCCEEEEEEEECCCCHHHHHEEEECCCCCHHHCCCCCCCCEEEEEEECCCCCE
IELIERHSTRDASRP
EEEEECCCCCCCCCC
>Mature Secondary Structure
MRLLHTMLRVGDLQRSIDFYTRVLGMQLLRQSDNPEYKYRLAFVGYGPESETAVLELTYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCEEEEEEEEC
YGVDKYDLGTAYGHIALETDNAAAACDRIRVAGGKVAREAGPVKGGTTVIAFVEDPDGYK
CCCCCEECCCEEEEEEEECCCCHHHHHEEEECCCCCHHHCCCCCCCCEEEEEEECCCCCE
IELIERHSTRDASRP
EEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]