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The map label for this gene is gdhA [H]

Identifier: 73540162

GI number: 73540162

Start: 502165

End: 503472

Strand: Direct

Name: gdhA [H]

Synonym: Reut_A0456

Alternate gene names: 73540162

Gene position: 502165-503472 (Clockwise)

Preceding gene: 73540161

Following gene: 73540163

Centisome position: 13.19

GC content: 65.37

Gene sequence:

>1308_bases
ATGTCCTCCGCAGCACCGACCCATACTGCTGGCCAAAAGCACGCACTGCCGTCCTACCTCAACGCCGACAACCTCGGCCC
CTGGGGCATCTACCTGCAACAAGTCGACCGTGTCACGCCTTACCTGGGCTCGCTGGCACGCTGGGTTGAAACCCTCAAGC
GCCCCAAGCGCGCGATGATCGTCGACGTACCCATCGAGCTCGATAACGGCACCATCGCCCACTACGAGGGCTACCGCGTG
CAGCACAACCTGTCGCGCGGCCCGGGGAAGGGCGGCGTGCGCTTCCACCAGGACGTGACGCTGTCGGAAGTCATGGCGCT
GTCGGCATGGATGTCGGTGAAGAACGCCGCGGTGAACGTGCCCTACGGCGGTGCCAAGGGTGGCATCCGCGTGGACCCGC
GCACCCTGTCGCACGCCGAGCTGGAACGCCTCACGCGCCGCTACACCAGCGAAATCAACATCATCATCGGGCCGAGCAAG
GACATCCCGGCGCCGGACGTCAACACCAATGCCCAGGTGATGGCGTGGATGATGGACACGTATTCGATGAACTCCGGCAG
CACCGCGACCGGTGTCGTGACCGGCAAGCCGATCTCGCTGGGCGGCTCGCTGGGCCGCCATGAGGCGACCGGCCGCGGCG
TGTTCGTGGTGGGTTCCGAGGCCGCGCGCAATATCGGCCTGGAGATCAAGGGTGCCCGCGTGGCCGTGCAGGGCTTCGGC
AACGTCGGCGCCGTGGCCGCGAAACTGTTCCAGGAGGCGGGCGCCAAGGTGGTGGCCGTGCAGGACCATCGCGTCTCGCT
GTACAACCCGGCCGGCCTGGACGTGCCGGCAATGATGGAATACGCCTCGCACAGCGGCACCGTCGACGGCTTCCAGGCGG
AAACCATTTCGTCGGAGCAGTTCTGGCAGGTGGACTGCGACATCCTGATCCCGGCCGCGCTGGAAGGCCAGATCACCGCG
AAGAATGCGCCCCAGATCAAGGCACGCCTGGTCATCGAAGGTGCAAACGGCCCGACCACGCCCGAGGCCGATGACATCCT
GCGCGAGCGCAATATCCTCGTGGCGCCGGACGTGATCGCCAACGCCGGCGGCGTGACCGTGTCGTATTTCGAGTGGGTGC
AGGATTTTTCCAGCTTCTTCTGGACCGAAGAGGAAATCAACGAACGCCTGGTACGGATCATGCAAGAGGCGTTCCGCGCT
ATCTGGCAGGTCGCGCAGGACAACAAGGTAACGCTGCGCACGGCCGCGTTTATCGTGGCCTGTACGCGCATCCTGCAGGC
GCGCGAAATGCGAGGCCTGTACCCCTGA

Upstream 100 bases:

>100_bases
CGTTATGCGCATCTTGCATGACCGGATGAGCAAACGGCATTGGATTCCATATGTGCCCCACCCCTAAGCTTGCGGGCATT
CTCCACCCCGGAACCCACTG

Downstream 100 bases:

>100_bases
TGCAGAGGTCGTTGCGCGCTCAACCTCTCTGTTGCGGCGCAGCAACGTCAGCCTGACCATCAGACGCGGCCCGGGGAAAG
CTTCCTCGGGCCGCGTTGTC

Product: Glu/Leu/Phe/Val dehydrogenase, C terminal:Glu/Leu/Phe/Val dehydrogenase, dimerisation region

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSSAAPTHTAGQKHALPSYLNADNLGPWGIYLQQVDRVTPYLGSLARWVETLKRPKRAMIVDVPIELDNGTIAHYEGYRV
QHNLSRGPGKGGVRFHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRTLSHAELERLTRRYTSEINIIIGPSK
DIPAPDVNTNAQVMAWMMDTYSMNSGSTATGVVTGKPISLGGSLGRHEATGRGVFVVGSEAARNIGLEIKGARVAVQGFG
NVGAVAAKLFQEAGAKVVAVQDHRVSLYNPAGLDVPAMMEYASHSGTVDGFQAETISSEQFWQVDCDILIPAALEGQITA
KNAPQIKARLVIEGANGPTTPEADDILRERNILVAPDVIANAGGVTVSYFEWVQDFSSFFWTEEEINERLVRIMQEAFRA
IWQVAQDNKVTLRTAAFIVACTRILQAREMRGLYP

Sequences:

>Translated_435_residues
MSSAAPTHTAGQKHALPSYLNADNLGPWGIYLQQVDRVTPYLGSLARWVETLKRPKRAMIVDVPIELDNGTIAHYEGYRV
QHNLSRGPGKGGVRFHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRTLSHAELERLTRRYTSEINIIIGPSK
DIPAPDVNTNAQVMAWMMDTYSMNSGSTATGVVTGKPISLGGSLGRHEATGRGVFVVGSEAARNIGLEIKGARVAVQGFG
NVGAVAAKLFQEAGAKVVAVQDHRVSLYNPAGLDVPAMMEYASHSGTVDGFQAETISSEQFWQVDCDILIPAALEGQITA
KNAPQIKARLVIEGANGPTTPEADDILRERNILVAPDVIANAGGVTVSYFEWVQDFSSFFWTEEEINERLVRIMQEAFRA
IWQVAQDNKVTLRTAAFIVACTRILQAREMRGLYP
>Mature_434_residues
SSAAPTHTAGQKHALPSYLNADNLGPWGIYLQQVDRVTPYLGSLARWVETLKRPKRAMIVDVPIELDNGTIAHYEGYRVQ
HNLSRGPGKGGVRFHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRTLSHAELERLTRRYTSEINIIIGPSKD
IPAPDVNTNAQVMAWMMDTYSMNSGSTATGVVTGKPISLGGSLGRHEATGRGVFVVGSEAARNIGLEIKGARVAVQGFGN
VGAVAAKLFQEAGAKVVAVQDHRVSLYNPAGLDVPAMMEYASHSGTVDGFQAETISSEQFWQVDCDILIPAALEGQITAK
NAPQIKARLVIEGANGPTTPEADDILRERNILVAPDVIANAGGVTVSYFEWVQDFSSFFWTEEEINERLVRIMQEAFRAI
WQVAQDNKVTLRTAAFIVACTRILQAREMRGLYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=369, Percent_Identity=43.0894308943089, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI4885281, Length=370, Percent_Identity=42.4324324324324, Blast_Score=288, Evalue=6e-78,
Organism=Escherichia coli, GI1788059, Length=364, Percent_Identity=32.967032967033, Blast_Score=177, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17544676, Length=390, Percent_Identity=38.2051282051282, Blast_Score=265, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6319256, Length=383, Percent_Identity=31.5926892950392, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6324951, Length=382, Percent_Identity=29.3193717277487, Blast_Score=140, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24649283, Length=455, Percent_Identity=38.6813186813187, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI45553475, Length=358, Percent_Identity=43.5754189944134, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI45549226, Length=361, Percent_Identity=43.213296398892, Blast_Score=282, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24649460, Length=311, Percent_Identity=43.0868167202572, Blast_Score=239, Evalue=3e-63,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 47200; Mature: 47069

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAAPTHTAGQKHALPSYLNADNLGPWGIYLQQVDRVTPYLGSLARWVETLKRPKRAMI
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
VDVPIELDNGTIAHYEGYRVQHNLSRGPGKGGVRFHQDVTLSEVMALSAWMSVKNAAVNV
EEEEEEECCCEEEEECCEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCEEEC
PYGGAKGGIRVDPRTLSHAELERLTRRYTSEINIIIGPSKDIPAPDVNTNAQVMAWMMDT
CCCCCCCCEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHEEEEEHHH
YSMNSGSTATGVVTGKPISLGGSLGRHEATGRGVFVVGSEAARNIGLEIKGARVAVQGFG
HCCCCCCCEEEEEECCCEECCCCCCCCCCCCCEEEEECCHHHHCCCEEEECCEEEEECCC
NVGAVAAKLFQEAGAKVVAVQDHRVSLYNPAGLDVPAMMEYASHSGTVDGFQAETISSEQ
CHHHHHHHHHHHCCCEEEEEECCEEEEECCCCCCHHHHHHHHHCCCCCCCEEECCCCCCC
FWQVDCDILIPAALEGQITAKNAPQIKARLVIEGANGPTTPEADDILRERNILVAPDVIA
EEEECCCEEECCCCCCEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHCCEEECCHHHH
NAGGVTVSYFEWVQDFSSFFWTEEEINERLVRIMQEAFRAIWQVAQDNKVTLRTAAFIVA
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
CTRILQAREMRGLYP
HHHHHHHHHHCCCCC
>Mature Secondary Structure 
SSAAPTHTAGQKHALPSYLNADNLGPWGIYLQQVDRVTPYLGSLARWVETLKRPKRAMI
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
VDVPIELDNGTIAHYEGYRVQHNLSRGPGKGGVRFHQDVTLSEVMALSAWMSVKNAAVNV
EEEEEEECCCEEEEECCEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCEEEC
PYGGAKGGIRVDPRTLSHAELERLTRRYTSEINIIIGPSKDIPAPDVNTNAQVMAWMMDT
CCCCCCCCEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHEEEEEHHH
YSMNSGSTATGVVTGKPISLGGSLGRHEATGRGVFVVGSEAARNIGLEIKGARVAVQGFG
HCCCCCCCEEEEEECCCEECCCCCCCCCCCCCEEEEECCHHHHCCCEEEECCEEEEECCC
NVGAVAAKLFQEAGAKVVAVQDHRVSLYNPAGLDVPAMMEYASHSGTVDGFQAETISSEQ
CHHHHHHHHHHHCCCEEEEEECCEEEEECCCCCCHHHHHHHHHCCCCCCCEEECCCCCCC
FWQVDCDILIPAALEGQITAKNAPQIKARLVIEGANGPTTPEADDILRERNILVAPDVIA
EEEECCCEEECCCCCCEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHCCEEECCHHHH
NAGGVTVSYFEWVQDFSSFFWTEEEINERLVRIMQEAFRAIWQVAQDNKVTLRTAAFIVA
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
CTRILQAREMRGLYP
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]