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The map label for this gene is paaF [C]

Identifier: 73540156

GI number: 73540156

Start: 496466

End: 497242

Strand: Direct

Name: paaF [C]

Synonym: Reut_A0450

Alternate gene names: 73540156

Gene position: 496466-497242 (Clockwise)

Preceding gene: 73540155

Following gene: 73540158

Centisome position: 13.04

GC content: 64.35

Gene sequence:

>777_bases
ATGAGCATCAGCACCAGCATTGAACACGGCATCCTGACGATCGAGTTCGATCGGGCCGGAAAGAAGAACGCCATCACGGC
GGCGATGTACCAGGCCATGGCTGACGCGCTGCGCGCGGCCGAGACGGACAGCGCGGTCCGCGTGATTCTGCTCCGCGGCA
AGGACGAGATCTTCACGGCAGGCAACGACCTCGAAGATTTCATGCAGCGGCCGCCGACCAGCGGTGAAGGCGCGGAGCAA
CCGCCGGTGTTCCAGTTCCTGTACCAGATCAGCCATGCGAGCAAGCCGATCGTCGCGGCGGTCAGCGGCGCGGCCGTGGG
CGTGGGCACCACGATGCTGCTGCACTGCGAATTCGTCTACGCGTCGGAAACGGCGAAGCTGTCGCTGCCGTTCGCGCAGC
TTGGGCTGTGCCCGGAAGCTGCATCGAGCCTGCTGCTGCCACGCCTGGTCGGCTACCAGCGCGCTGCTGAAAAACTGATG
CTCGGTGAGCCCTTCGGTGCGAAGGAAGCATACGAGATCGGGCTCGTCACGCGCGTGTTGCCCGTGGCCGAACTGCATGC
CTTTGCGCTGCAGCAGGCCCGCAAGCTGGCCGCGCTGCCGGCGTCGTCGCTGCGCGAGACCAAACGGCTGATGAAGGGCA
ATGACGTGGAAGCCGTGGAGAAGAAGATGGCCGATGAGGGCGCGGTGTTCCGGCGCATGCTGGTGGCACCGGAAGCGAAG
GAAGCGTTCTCGGCGTTCTTCGAGAAGCGGAAGCCGGATTTTACGAAGTTCAGTTGA

Upstream 100 bases:

>100_bases
CGCGCGTTTGGCCTGAGCTATCTTTCCGAGGAGCCGCTGCCGCTTGCATAGCGATGGCGGCGCAGTCCTTGCAGCAACGC
ATCACCACGAGAGGCATTCC

Downstream 100 bases:

>100_bases
GCCGGCGAGGGGGCAGGCTCCTGGCAAGCCTGCTTCACCGTGAGACCAACTCCCCTCCGCGCGCGCGGGAGAGGGTAGAC
ACCATCAAGTCGGCAACACC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSISTSIEHGILTIEFDRAGKKNAITAAMYQAMADALRAAETDSAVRVILLRGKDEIFTAGNDLEDFMQRPPTSGEGAEQ
PPVFQFLYQISHASKPIVAAVSGAAVGVGTTMLLHCEFVYASETAKLSLPFAQLGLCPEAASSLLLPRLVGYQRAAEKLM
LGEPFGAKEAYEIGLVTRVLPVAELHAFALQQARKLAALPASSLRETKRLMKGNDVEAVEKKMADEGAVFRRMLVAPEAK
EAFSAFFEKRKPDFTKFS

Sequences:

>Translated_258_residues
MSISTSIEHGILTIEFDRAGKKNAITAAMYQAMADALRAAETDSAVRVILLRGKDEIFTAGNDLEDFMQRPPTSGEGAEQ
PPVFQFLYQISHASKPIVAAVSGAAVGVGTTMLLHCEFVYASETAKLSLPFAQLGLCPEAASSLLLPRLVGYQRAAEKLM
LGEPFGAKEAYEIGLVTRVLPVAELHAFALQQARKLAALPASSLRETKRLMKGNDVEAVEKKMADEGAVFRRMLVAPEAK
EAFSAFFEKRKPDFTKFS
>Mature_257_residues
SISTSIEHGILTIEFDRAGKKNAITAAMYQAMADALRAAETDSAVRVILLRGKDEIFTAGNDLEDFMQRPPTSGEGAEQP
PVFQFLYQISHASKPIVAAVSGAAVGVGTTMLLHCEFVYASETAKLSLPFAQLGLCPEAASSLLLPRLVGYQRAAEKLML
GEPFGAKEAYEIGLVTRVLPVAELHAFALQQARKLAALPASSLRETKRLMKGNDVEAVEKKMADEGAVFRRMLVAPEAKE
AFSAFFEKRKPDFTKFS

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI260274832, Length=252, Percent_Identity=32.1428571428571, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI45643119, Length=252, Percent_Identity=32.1428571428571, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI260275230, Length=252, Percent_Identity=32.1428571428571, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI194097323, Length=246, Percent_Identity=30.4878048780488, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI4757968, Length=242, Percent_Identity=27.6859504132231, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI49355787, Length=242, Percent_Identity=27.6859504132231, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI25453481, Length=240, Percent_Identity=26.25, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI51538573, Length=240, Percent_Identity=26.25, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI4757966, Length=229, Percent_Identity=26.6375545851528, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI51538581, Length=229, Percent_Identity=26.6375545851528, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI31542718, Length=260, Percent_Identity=28.8461538461538, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI221136756, Length=242, Percent_Identity=24.3801652892562, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI221136753, Length=242, Percent_Identity=24.3801652892562, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI221307494, Length=242, Percent_Identity=24.3801652892562, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI116235487, Length=196, Percent_Identity=30.1020408163265, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI70995211, Length=193, Percent_Identity=26.9430051813472, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI68989263, Length=205, Percent_Identity=30.7317073170732, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI4502327, Length=253, Percent_Identity=28.0632411067194, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=30.5220883534137, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=31.2741312741313, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788597, Length=253, Percent_Identity=28.8537549407115, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=31.9230769230769, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI87082183, Length=262, Percent_Identity=25.5725190839695, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1790281, Length=181, Percent_Identity=28.7292817679558, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1788682, Length=194, Percent_Identity=29.8969072164948, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17535521, Length=254, Percent_Identity=29.9212598425197, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI25145438, Length=256, Percent_Identity=29.6875, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17549921, Length=216, Percent_Identity=32.8703703703704, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17554946, Length=248, Percent_Identity=26.6129032258064, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17560910, Length=197, Percent_Identity=29.9492385786802, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=198, Percent_Identity=29.2929292929293, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17540714, Length=195, Percent_Identity=27.6923076923077, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=202, Percent_Identity=24.2574257425743, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=200, Percent_Identity=30, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25144276, Length=170, Percent_Identity=30.5882352941176, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=170, Percent_Identity=30.5882352941176, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=239, Percent_Identity=24.2677824267782, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6323314, Length=237, Percent_Identity=25.3164556962025, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24654903, Length=242, Percent_Identity=30.9917355371901, Blast_Score=137, Evalue=7e-33,
Organism=Drosophila melanogaster, GI20129971, Length=247, Percent_Identity=26.7206477732794, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24653477, Length=247, Percent_Identity=26.7206477732794, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24653139, Length=253, Percent_Identity=31.2252964426877, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21357171, Length=261, Percent_Identity=25.6704980842912, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922422, Length=260, Percent_Identity=24.6153846153846, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19920382, Length=183, Percent_Identity=27.8688524590164, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24650670, Length=202, Percent_Identity=28.7128712871287, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27985; Mature: 27853

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISTSIEHGILTIEFDRAGKKNAITAAMYQAMADALRAAETDSAVRVILLRGKDEIFTA
CCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEC
GNDLEDFMQRPPTSGEGAEQPPVFQFLYQISHASKPIVAAVSGAAVGVGTTMLLHCEFVY
CCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHEEEEEEEEEE
ASETAKLSLPFAQLGLCPEAASSLLLPRLVGYQRAAEKLMLGEPFGAKEAYEIGLVTRVL
ECCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
PVAELHAFALQQARKLAALPASSLRETKRLMKGNDVEAVEKKMADEGAVFRRMLVAPEAK
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
EAFSAFFEKRKPDFTKFS
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SISTSIEHGILTIEFDRAGKKNAITAAMYQAMADALRAAETDSAVRVILLRGKDEIFTA
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEC
GNDLEDFMQRPPTSGEGAEQPPVFQFLYQISHASKPIVAAVSGAAVGVGTTMLLHCEFVY
CCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHEEEEEEEEEE
ASETAKLSLPFAQLGLCPEAASSLLLPRLVGYQRAAEKLMLGEPFGAKEAYEIGLVTRVL
ECCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
PVAELHAFALQQARKLAALPASSLRETKRLMKGNDVEAVEKKMADEGAVFRRMLVAPEAK
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
EAFSAFFEKRKPDFTKFS
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA