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The map label for this gene is fadA [H]

Identifier: 73540154

GI number: 73540154

Start: 494078

End: 495274

Strand: Direct

Name: fadA [H]

Synonym: Reut_A0448

Alternate gene names: 73540154

Gene position: 494078-495274 (Clockwise)

Preceding gene: 73540153

Following gene: 73540155

Centisome position: 12.98

GC content: 65.33

Gene sequence:

>1197_bases
ATGAAACAACTGCAAGACGCCTACATCGTTGCCGCGACTCGCTCTCCGATCGGCAAGGCGCCGAAGGGCGCGTTCAAGAA
CACGCGTCCGGACGACCTGCTCGCGACGATCCTGAAAGCCGCCGTGGCGCAGGTGCCGAACCTCGATCCGAAGCTGATCG
AAGATGCCATCGTCGGCTGCGCGATTCCCGAAGCGCAGCAGGGCCTGAACGTGGCGCGTATCGGCGCGCTGCTGTCGGGC
CTGCCGAACACGGTGGGCGGCATCACCGTCAACCGCTTCTGCGCCTCCGGCGTGAGTGCCGTGGCGATGGCCGCGGACCG
CATCCGCGTGGGCGAGTCCGACGTGATGATCGCCGCCGGCGTGGAAACGATGAGCATGGTGCCGATGATGGGCAACTCGC
CGTCGATGTCGCCGGACATCTTCACGCGGGACGAAAACGTTGGCATCGCGTACGGCATGGGCCTGACCGCCGAGAAGGTC
GCTCAGCAATGGAATGTCAGCCGTGAGGACCAGGATGCGTTTTCGCTGGCTTCGCACCAGAAGGCCATCGCTGCCCAGCA
GGCCGGCGAGTTCAACGACGAGATCACGCCGATCGAAATCGTCGAGAAATTCCCGAACCTGGCTACGGGAGAGGTGAGCG
TCAAGACGCGCACGATCTCGCTCGACGAAGGCCCGCGCCCGGAGACCTCGCTCGAAGGCCTTGGCAAGCTGCGCCCGGTG
TTCGCAAACAAGGGTAGCGTGACCGCCGGCAACAGCTCGCAGACGTCCGACGGCGCCGGCGCGCTGATCCTGGTCTCCGA
GAAGATCCTGAAGCAGTTCAACCTGGTGCCGCTCGCACGCTTCGTGTCGTTCGCGGTGCGCGGCGTGCCGCCGGAGATCA
TGGGTATCGGCCCGAAGGAAGCGATTCCTGCGGTGTTGAAGGCAGCCGGGCTGACGCAGGACCAGATCGACTGGATCGAA
CTGAACGAAGCGTTTGCCGCGCAATCGCTGGCCGTGATGCGTGATCTTGAACTCGACCCGTCCAAGGTCAACCGCATGGG
CGGTGCCATTGCGCTCGGCCACCCGCTCGGCGCGACGGGCGCGATCCGCTCGGCGACTGTGGTGCACGCGCTGCGTCGCC
ACAACCTGAAGTACGGCATGGTGACCATGTGCGTCGGTACGGGCATGGGCGCGGCCGGCATCTTTGAGCGCGTCTGA

Upstream 100 bases:

>100_bases
TCGTCGACCTGCTTGGCACCAGCAAGACGCAGGAACGCATCATGGGCATGCTGCAGACCGGCAAGCCCGTCCGCAACTAA
GGCAGCGAGGAAACGACATC

Downstream 100 bases:

>100_bases
AGAAACGCGCCAGCAGGGATGCTGCGGCTTAAGGCTGTCAAAGCGCATCAAACGCAGCATCCCATCCTTCTGTCAGGTGA
AGAAAAGCAGTGCCAACGTC

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKQLQDAYIVAATRSPIGKAPKGAFKNTRPDDLLATILKAAVAQVPNLDPKLIEDAIVGCAIPEAQQGLNVARIGALLSG
LPNTVGGITVNRFCASGVSAVAMAADRIRVGESDVMIAAGVETMSMVPMMGNSPSMSPDIFTRDENVGIAYGMGLTAEKV
AQQWNVSREDQDAFSLASHQKAIAAQQAGEFNDEITPIEIVEKFPNLATGEVSVKTRTISLDEGPRPETSLEGLGKLRPV
FANKGSVTAGNSSQTSDGAGALILVSEKILKQFNLVPLARFVSFAVRGVPPEIMGIGPKEAIPAVLKAAGLTQDQIDWIE
LNEAFAAQSLAVMRDLELDPSKVNRMGGAIALGHPLGATGAIRSATVVHALRRHNLKYGMVTMCVGTGMGAAGIFERV

Sequences:

>Translated_398_residues
MKQLQDAYIVAATRSPIGKAPKGAFKNTRPDDLLATILKAAVAQVPNLDPKLIEDAIVGCAIPEAQQGLNVARIGALLSG
LPNTVGGITVNRFCASGVSAVAMAADRIRVGESDVMIAAGVETMSMVPMMGNSPSMSPDIFTRDENVGIAYGMGLTAEKV
AQQWNVSREDQDAFSLASHQKAIAAQQAGEFNDEITPIEIVEKFPNLATGEVSVKTRTISLDEGPRPETSLEGLGKLRPV
FANKGSVTAGNSSQTSDGAGALILVSEKILKQFNLVPLARFVSFAVRGVPPEIMGIGPKEAIPAVLKAAGLTQDQIDWIE
LNEAFAAQSLAVMRDLELDPSKVNRMGGAIALGHPLGATGAIRSATVVHALRRHNLKYGMVTMCVGTGMGAAGIFERV
>Mature_398_residues
MKQLQDAYIVAATRSPIGKAPKGAFKNTRPDDLLATILKAAVAQVPNLDPKLIEDAIVGCAIPEAQQGLNVARIGALLSG
LPNTVGGITVNRFCASGVSAVAMAADRIRVGESDVMIAAGVETMSMVPMMGNSPSMSPDIFTRDENVGIAYGMGLTAEKV
AQQWNVSREDQDAFSLASHQKAIAAQQAGEFNDEITPIEIVEKFPNLATGEVSVKTRTISLDEGPRPETSLEGLGKLRPV
FANKGSVTAGNSSQTSDGAGALILVSEKILKQFNLVPLARFVSFAVRGVPPEIMGIGPKEAIPAVLKAAGLTQDQIDWIE
LNEAFAAQSLAVMRDLELDPSKVNRMGGAIALGHPLGATGAIRSATVVHALRRHNLKYGMVTMCVGTGMGAAGIFERV

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=392, Percent_Identity=44.3877551020408, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI167614485, Length=406, Percent_Identity=39.4088669950739, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI148539872, Length=400, Percent_Identity=36.75, Blast_Score=232, Evalue=6e-61,
Organism=Homo sapiens, GI4557237, Length=405, Percent_Identity=34.0740740740741, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI4504327, Length=419, Percent_Identity=30.7875894988067, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI194353979, Length=245, Percent_Identity=36.3265306122449, Blast_Score=136, Evalue=3e-32,
Organism=Escherichia coli, GI1787663, Length=415, Percent_Identity=43.1325301204819, Blast_Score=295, Evalue=5e-81,
Organism=Escherichia coli, GI48994986, Length=402, Percent_Identity=41.7910447761194, Blast_Score=278, Evalue=3e-76,
Organism=Escherichia coli, GI1788554, Length=406, Percent_Identity=40.8866995073892, Blast_Score=265, Evalue=4e-72,
Organism=Escherichia coli, GI87082165, Length=404, Percent_Identity=38.8613861386139, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1788683, Length=423, Percent_Identity=30.7328605200946, Blast_Score=155, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI133906874, Length=401, Percent_Identity=40.6483790523691, Blast_Score=265, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI17535921, Length=407, Percent_Identity=34.1523341523341, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI25147385, Length=398, Percent_Identity=31.4070351758794, Blast_Score=186, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17551802, Length=446, Percent_Identity=28.9237668161435, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17535917, Length=394, Percent_Identity=28.4263959390863, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6322031, Length=400, Percent_Identity=42.75, Blast_Score=289, Evalue=7e-79,
Organism=Saccharomyces cerevisiae, GI6325229, Length=413, Percent_Identity=36.5617433414044, Blast_Score=227, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24655093, Length=408, Percent_Identity=38.2352941176471, Blast_Score=265, Evalue=3e-71,
Organism=Drosophila melanogaster, GI17648125, Length=402, Percent_Identity=40.7960199004975, Blast_Score=261, Evalue=7e-70,
Organism=Drosophila melanogaster, GI24640423, Length=411, Percent_Identity=32.8467153284672, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI17137578, Length=441, Percent_Identity=31.0657596371882, Blast_Score=162, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 41766; Mature: 41766

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLQDAYIVAATRSPIGKAPKGAFKNTRPDDLLATILKAAVAQVPNLDPKLIEDAIVGC
CCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
AIPEAQQGLNVARIGALLSGLPNTVGGITVNRFCASGVSAVAMAADRIRVGESDVMIAAG
CCCHHHHCCCHHHHHHHHHCCCCHHCCCHHHHHHHCCHHHHHHHHHHHEECCCCEEEEEC
VETMSMVPMMGNSPSMSPDIFTRDENVGIAYGMGLTAEKVAQQWNVSREDQDAFSLASHQ
CHHHHHCCCCCCCCCCCCCCEECCCCCCEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHH
KAIAAQQAGEFNDEITPIEIVEKFPNLATGEVSVKTRTISLDEGPRPETSLEGLGKLRPV
HHHHHHHCCCCCCCCCHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHCHH
FANKGSVTAGNSSQTSDGAGALILVSEKILKQFNLVPLARFVSFAVRGVPPEIMGIGPKE
HCCCCCEECCCCCCCCCCCCEEEEEEHHHHHHHCCHHHHHHHHHHHCCCCHHHHCCCCHH
AIPAVLKAAGLTQDQIDWIELNEAFAAQSLAVMRDLELDPSKVNRMGGAIALGHPLGATG
HHHHHHHHCCCCHHCCHHEEHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEECCCCCCCC
AIRSATVVHALRRHNLKYGMVTMCVGTGMGAAGIFERV
HHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure
MKQLQDAYIVAATRSPIGKAPKGAFKNTRPDDLLATILKAAVAQVPNLDPKLIEDAIVGC
CCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
AIPEAQQGLNVARIGALLSGLPNTVGGITVNRFCASGVSAVAMAADRIRVGESDVMIAAG
CCCHHHHCCCHHHHHHHHHCCCCHHCCCHHHHHHHCCHHHHHHHHHHHEECCCCEEEEEC
VETMSMVPMMGNSPSMSPDIFTRDENVGIAYGMGLTAEKVAQQWNVSREDQDAFSLASHQ
CHHHHHCCCCCCCCCCCCCCEECCCCCCEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHH
KAIAAQQAGEFNDEITPIEIVEKFPNLATGEVSVKTRTISLDEGPRPETSLEGLGKLRPV
HHHHHHHCCCCCCCCCHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHCHH
FANKGSVTAGNSSQTSDGAGALILVSEKILKQFNLVPLARFVSFAVRGVPPEIMGIGPKE
HCCCCCEECCCCCCCCCCCCEEEEEEHHHHHHHCCHHHHHHHHHHHCCCCHHHHCCCCHH
AIPAVLKAAGLTQDQIDWIELNEAFAAQSLAVMRDLELDPSKVNRMGGAIALGHPLGATG
HHHHHHHHCCCCHHCCHHEEHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEECCCCCCCC
AIRSATVVHALRRHNLKYGMVTMCVGTGMGAAGIFERV
HHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]