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The map label for this gene is (cry1Jb [H]

Identifier: 73540082

GI number: 73540082

Start: 410464

End: 411945

Strand: Reverse

Name: (cry1Jb [H]

Synonym: Reut_A0376

Alternate gene names: 73540082

Gene position: 411945-410464 (Counterclockwise)

Preceding gene: 73540086

Following gene: 73540076

Centisome position: 10.82

GC content: 62.35

Gene sequence:

>1482_bases
ATGCTGACTCGACGCGACTTACTCAGATCCATCCCTTCCGCCGCCCTGATGGCAATGGGCCCCGCTTTCCCGGCGCACGC
AGATGGCGCCCTCGGGAAACTGCCCGTCACCGATCCGATGGAAGACCTGAAGACCATCCTCGAGGTCGTGCTTGCGGAGG
TCCCTGTCGTCGGCGGCGCCATGTCCGCCCTGGTCGGACTACTTTGGCCGGTTGAACCTGCGGACGTCTGGGAGGAAATC
AGGCAGAAGGTCGAAGCCCTGATCGAGCAGAAGATCAATGACGCGGTCTACAGTTTGCTGAAGAGCAAGCTCGACGGCAT
CGGCAACACGCTGAAGCTGTACCTGAACGCGGCGGCGACCGGCGACACATCAAACATGCGTATGCAGTTCATCGCCACCA
ACACCCAGATGACGCTGGCGGCATCGGAATTCCGCAACCCGGATTTCCAATGGGTACTGGCCCCGTTGTTCGCCATCTTC
TCCCAGCTGCATGCCGTGCTGCTGCGCGACTGCGTGCTGCATGGCAGGGACTGGGGATGGAACACCGCGACCTACGAGGG
CTATGTCAGGCTCGCGAGCGATACGATCAACGAGTACCACGCCTATCTGGACGCCGTCGTCAGGAAGGAGCAGGACCGGC
TGGCACCGGCCGCGCCCACGAGCCCGGGCAAGCACTACACCGCCATCTACAACTACTGGCAATCCTTTTCGCAGAAGAAG
CAGGTCCTGTTCGGCGACTACATGCTGCTGCTGAACGCCCTGGACCCGATCCGGAACCCCGGTCAGGTAAAGACCATCCC
GTTTCCCGATGTGTTCAGCGCCGCCTATGGCACGGCGGATGACTGGGATGCCACGTGCCGCGCCTGGGCCGGTGCCGTCG
CGACACCGTTCAGCCTGCCCGCCGCCAACATCTCCGAAATCTATATCGAGCTGTTCAACTTCACGCCCCGGATCGTCGAC
GTCAGCTATCCGGCCGGGAGCGGGCCGAAGGTGTGGGGCGGCAACCGGCAGGACCGCACCGGCATCATCGCGGATCGAGT
GAGTGGCGTGGAGCAAAAAACGATCAGCATTCCGCGGTCCACGGACGGCAAGACATTCAACATCGCGGGCGCATCGATCC
GAGCCGGCAGTATTCCAACCGCGGTATCGCTGGTCATGGACGACGGCAAATCGATTGCGCTCTGGGACCGCCGCGATCTC
GGCGGCCAGCAGTTCTACGTGTCTGTTCCCGGCAGAAAGCTCACGACGCTAAACATGTGGTCGCGCAGCCGGTTCTATGA
CAATGACCTTGGCTGCATCATCTTCGGCTTCAGCCGCGATCCGAGCGATATCCCGCCTGCTGTCAGGGAGGCGCTCTACG
TCACCGCGATCCAGCCGCCCAGTGTCGGGCCGAGGTTCCTGCCATCGACAGTGTCGCGCGATGCACAGTACAAGCGCGAT
CGCTTCTGGCAGCAAGTACGCAGCGCCAGACCAGCCCAATAA

Upstream 100 bases:

>100_bases
CCGCCTTCTGTTTGAGTAGCGTCCGAAATTCGTAACTCCGCGAGCCCTGTCGCCCTACGAACGATGCCTAGCGCTCGTGG
ACATCCATGGAGAATTCCGA

Downstream 100 bases:

>100_bases
CAAGAACGTCGACGTACCAGCAACCGCTCGAACAAAAACGCCCGGCTCGCAAGCCGGGCGTGTCTGATAGCGTCATGATT
CCGCTGTCAGTGGCTTGCCC

Product: twin-arginine translocation pathway signal

Products: NA

Alternate protein names: 134 kDa crystal protein; Crystaline entomocidal protoxin; Insecticidal delta-endotoxin CryIJ(b) [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MLTRRDLLRSIPSAALMAMGPAFPAHADGALGKLPVTDPMEDLKTILEVVLAEVPVVGGAMSALVGLLWPVEPADVWEEI
RQKVEALIEQKINDAVYSLLKSKLDGIGNTLKLYLNAAATGDTSNMRMQFIATNTQMTLAASEFRNPDFQWVLAPLFAIF
SQLHAVLLRDCVLHGRDWGWNTATYEGYVRLASDTINEYHAYLDAVVRKEQDRLAPAAPTSPGKHYTAIYNYWQSFSQKK
QVLFGDYMLLLNALDPIRNPGQVKTIPFPDVFSAAYGTADDWDATCRAWAGAVATPFSLPAANISEIYIELFNFTPRIVD
VSYPAGSGPKVWGGNRQDRTGIIADRVSGVEQKTISIPRSTDGKTFNIAGASIRAGSIPTAVSLVMDDGKSIALWDRRDL
GGQQFYVSVPGRKLTTLNMWSRSRFYDNDLGCIIFGFSRDPSDIPPAVREALYVTAIQPPSVGPRFLPSTVSRDAQYKRD
RFWQQVRSARPAQ

Sequences:

>Translated_493_residues
MLTRRDLLRSIPSAALMAMGPAFPAHADGALGKLPVTDPMEDLKTILEVVLAEVPVVGGAMSALVGLLWPVEPADVWEEI
RQKVEALIEQKINDAVYSLLKSKLDGIGNTLKLYLNAAATGDTSNMRMQFIATNTQMTLAASEFRNPDFQWVLAPLFAIF
SQLHAVLLRDCVLHGRDWGWNTATYEGYVRLASDTINEYHAYLDAVVRKEQDRLAPAAPTSPGKHYTAIYNYWQSFSQKK
QVLFGDYMLLLNALDPIRNPGQVKTIPFPDVFSAAYGTADDWDATCRAWAGAVATPFSLPAANISEIYIELFNFTPRIVD
VSYPAGSGPKVWGGNRQDRTGIIADRVSGVEQKTISIPRSTDGKTFNIAGASIRAGSIPTAVSLVMDDGKSIALWDRRDL
GGQQFYVSVPGRKLTTLNMWSRSRFYDNDLGCIIFGFSRDPSDIPPAVREALYVTAIQPPSVGPRFLPSTVSRDAQYKRD
RFWQQVRSARPAQ
>Mature_493_residues
MLTRRDLLRSIPSAALMAMGPAFPAHADGALGKLPVTDPMEDLKTILEVVLAEVPVVGGAMSALVGLLWPVEPADVWEEI
RQKVEALIEQKINDAVYSLLKSKLDGIGNTLKLYLNAAATGDTSNMRMQFIATNTQMTLAASEFRNPDFQWVLAPLFAIF
SQLHAVLLRDCVLHGRDWGWNTATYEGYVRLASDTINEYHAYLDAVVRKEQDRLAPAAPTSPGKHYTAIYNYWQSFSQKK
QVLFGDYMLLLNALDPIRNPGQVKTIPFPDVFSAAYGTADDWDATCRAWAGAVATPFSLPAANISEIYIELFNFTPRIVD
VSYPAGSGPKVWGGNRQDRTGIIADRVSGVEQKTISIPRSTDGKTFNIAGASIRAGSIPTAVSLVMDDGKSIALWDRRDL
GGQQFYVSVPGRKLTTLNMWSRSRFYDNDLGCIIFGFSRDPSDIPPAVREALYVTAIQPPSVGPRFLPSTVSRDAQYKRD
RFWQQVRSARPAQ

Specific function: Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae. Toxic to Plutella xylostella [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the delta endotoxin family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005638
- InterPro:   IPR001178
- InterPro:   IPR015790
- InterPro:   IPR005639
- InterPro:   IPR008979 [H]

Pfam domain/function: PF03944 Endotoxin_C; PF00555 Endotoxin_M; PF03945 Endotoxin_N [H]

EC number: NA

Molecular weight: Translated: 54443; Mature: 54443

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRRDLLRSIPSAALMAMGPAFPAHADGALGKLPVTDPMEDLKTILEVVLAEVPVVGGA
CCCHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHH
MSALVGLLWPVEPADVWEEIRQKVEALIEQKINDAVYSLLKSKLDGIGNTLKLYLNAAAT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
GDTSNMRMQFIATNTQMTLAASEFRNPDFQWVLAPLFAIFSQLHAVLLRDCVLHGRDWGW
CCCCCEEEEEEEECCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NTATYEGYVRLASDTINEYHAYLDAVVRKEQDRLAPAAPTSPGKHYTAIYNYWQSFSQKK
CCCCHHCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
QVLFGDYMLLLNALDPIRNPGQVKTIPFPDVFSAAYGTADDWDATCRAWAGAVATPFSLP
HHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCC
AANISEIYIELFNFTPRIVDVSYPAGSGPKVWGGNRQDRTGIIADRVSGVEQKTISIPRS
CCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHEEECCCC
TDGKTFNIAGASIRAGSIPTAVSLVMDDGKSIALWDRRDLGGQQFYVSVPGRKLTTLNMW
CCCCEEEECCCEEECCCCCCEEEEEECCCCEEEEEECCCCCCCEEEEECCCCEEEEEEHH
SRSRFYDNDLGCIIFGFSRDPSDIPPAVREALYVTAIQPPSVGPRFLPSTVSRDAQYKRD
HCCCCCCCCCCEEEEEECCCCCCCCHHHHHCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
RFWQQVRSARPAQ
HHHHHHHHCCCCC
>Mature Secondary Structure
MLTRRDLLRSIPSAALMAMGPAFPAHADGALGKLPVTDPMEDLKTILEVVLAEVPVVGGA
CCCHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHH
MSALVGLLWPVEPADVWEEIRQKVEALIEQKINDAVYSLLKSKLDGIGNTLKLYLNAAAT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
GDTSNMRMQFIATNTQMTLAASEFRNPDFQWVLAPLFAIFSQLHAVLLRDCVLHGRDWGW
CCCCCEEEEEEEECCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NTATYEGYVRLASDTINEYHAYLDAVVRKEQDRLAPAAPTSPGKHYTAIYNYWQSFSQKK
CCCCHHCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
QVLFGDYMLLLNALDPIRNPGQVKTIPFPDVFSAAYGTADDWDATCRAWAGAVATPFSLP
HHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCC
AANISEIYIELFNFTPRIVDVSYPAGSGPKVWGGNRQDRTGIIADRVSGVEQKTISIPRS
CCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHEEECCCC
TDGKTFNIAGASIRAGSIPTAVSLVMDDGKSIALWDRRDLGGQQFYVSVPGRKLTTLNMW
CCCCEEEECCCEEECCCCCCEEEEEECCCCEEEEEECCCCCCCEEEEECCCCEEEEEEHH
SRSRFYDNDLGCIIFGFSRDPSDIPPAVREALYVTAIQPPSVGPRFLPSTVSRDAQYKRD
HCCCCCCCCCCEEEEEECCCCCCCCHHHHHCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
RFWQQVRSARPAQ
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA