Click here to switch to the map view.

The map label for this gene is mutM

Identifier: 73540052

GI number: 73540052

Start: 375673

End: 376527

Strand: Direct

Name: mutM

Synonym: Reut_A0346

Alternate gene names: 73540052

Gene position: 375673-376527 (Clockwise)

Preceding gene: 73540045

Following gene: 73540053

Centisome position: 9.87

GC content: 70.06

Gene sequence:

>855_bases
ATGCCTGAATTGCCGGAGGTCGAAGTGACCCGGCGCGGCCTGCTGCCGCATGTGGTGGGGCGGCGTATTGCCGACGTAAC
GGTGCGCCACCGCGGCCTGCGCTGGCCGGTCGAGGACGACCTGGAGGCGCGCCTTGCCGGCCGCCTGGTTAGGCGGATCG
AGCGGCGCGGCAAATACCTGCTGCTCGAGTGCGTCGATGAAGCCACCGACGACGCCGGCTGGCTGCTGGTCCACCTCGGC
ATGACCGGAACGCTGCGCGTGCTGCCCGACGCGCCGCCGGCCGGCGCCCACGACCACCTGGATCTCGTGCTGGACGACGC
CGGCGGCAGCCGCATCGTGCTGCGCTTTCGCGATCCGCGCCGCTTCGGCGCCGTGCTGTGGAGCCCGCTGAGCGAAGCCA
TGCTGCCCGGGCACCCGCTGCTGCGCGGCCTGGGCATTGAGCCGTTCGATTCGCATTTCGACGGCAGCTGGCTGCATCGC
CATACGCGGGGCCGTTCCGCGGCGATCAAGACCGTACTGCTGGCCGGCAATATCGTCGTCGGGGTCGGCAACATCTACGC
TTCTGAAAGCCTCTTCCGCGCCGGCATCCGCCCGACAACGCCGGCTGGCCGGCTGAGCCTGGCGCGCTGCGAGCGGCTGG
CGCAGTCCGTGCGCGAAACCCTGGCGCAAGCCATCGAGCGGGGCGGCAGCACGCTGCGCGACTTTGTCGGCAGTGACGGC
GCCAGCGGCTATTTCCAGCTCGAATGCTTCGTCTACGACCGCGCCGGAGAGCCCTGCAAGGTTTGCGGCACGCCGGTGCG
GCAGATTGTCCAGGGCCAGCGCTCGACCTTCTACTGCACGCACTGCCAGCACTGA

Upstream 100 bases:

>100_bases
GGGGCATTGTAGCGTGCCGCTACAATGCGCTGCTTGCCGGCGCGGCCAGTCTGGCACGGTCCCGGCAGGAATTTGGAGCG
GTAACCAACGGAGAGGTTCG

Downstream 100 bases:

>100_bases
AGCGGGGCGCACGGGTGGGCACTGGCGTTCACTGGGCGCCATGTCGATGGCTGGGCGCAACACTCGGCCTGGCAAGGGGC
GCGAATTCCACAAAAGCGCC

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MPELPEVEVTRRGLLPHVVGRRIADVTVRHRGLRWPVEDDLEARLAGRLVRRIERRGKYLLLECVDEATDDAGWLLVHLG
MTGTLRVLPDAPPAGAHDHLDLVLDDAGGSRIVLRFRDPRRFGAVLWSPLSEAMLPGHPLLRGLGIEPFDSHFDGSWLHR
HTRGRSAAIKTVLLAGNIVVGVGNIYASESLFRAGIRPTTPAGRLSLARCERLAQSVRETLAQAIERGGSTLRDFVGSDG
ASGYFQLECFVYDRAGEPCKVCGTPVRQIVQGQRSTFYCTHCQH

Sequences:

>Translated_284_residues
MPELPEVEVTRRGLLPHVVGRRIADVTVRHRGLRWPVEDDLEARLAGRLVRRIERRGKYLLLECVDEATDDAGWLLVHLG
MTGTLRVLPDAPPAGAHDHLDLVLDDAGGSRIVLRFRDPRRFGAVLWSPLSEAMLPGHPLLRGLGIEPFDSHFDGSWLHR
HTRGRSAAIKTVLLAGNIVVGVGNIYASESLFRAGIRPTTPAGRLSLARCERLAQSVRETLAQAIERGGSTLRDFVGSDG
ASGYFQLECFVYDRAGEPCKVCGTPVRQIVQGQRSTFYCTHCQH
>Mature_283_residues
PELPEVEVTRRGLLPHVVGRRIADVTVRHRGLRWPVEDDLEARLAGRLVRRIERRGKYLLLECVDEATDDAGWLLVHLGM
TGTLRVLPDAPPAGAHDHLDLVLDDAGGSRIVLRFRDPRRFGAVLWSPLSEAMLPGHPLLRGLGIEPFDSHFDGSWLHRH
TRGRSAAIKTVLLAGNIVVGVGNIYASESLFRAGIRPTTPAGRLSLARCERLAQSVRETLAQAIERGGSTLRDFVGSDGA
SGYFQLECFVYDRAGEPCKVCGTPVRQIVQGQRSTFYCTHCQH

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=283, Percent_Identity=50.530035335689, Blast_Score=278, Evalue=4e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_CUPPJ (Q476F5)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294572.1
- ProteinModelPortal:   Q476F5
- SMR:   Q476F5
- GeneID:   3609587
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0346
- HOGENOM:   HBG690070
- OMA:   RMTGQLL
- ProtClustDB:   PRK01103
- BioCyc:   REUT264198:REUT_A0346-MONOMER
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 31376; Mature: 31245

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 58-58 ACT_SITE 274-274 BINDING 97-97 BINDING 120-120 BINDING 165-165

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVEVTRRGLLPHVVGRRIADVTVRHRGLRWPVEDDLEARLAGRLVRRIERRGKYL
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEE
LLECVDEATDDAGWLLVHLGMTGTLRVLPDAPPAGAHDHLDLVLDDAGGSRIVLRFRDPR
EEEEHHCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEECCCH
RFGAVLWSPLSEAMLPGHPLLRGLGIEPFDSHFDGSWLHRHTRGRSAAIKTVLLAGNIVV
HHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHHHHHEEECCEEE
GVGNIYASESLFRAGIRPTTPAGRLSLARCERLAQSVRETLAQAIERGGSTLRDFVGSDG
EECCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCC
ASGYFQLECFVYDRAGEPCKVCGTPVRQIVQGQRSTFYCTHCQH
CCCEEEEEEEEEECCCCCHHHCCHHHHHHHCCCCCEEEEECCCC
>Mature Secondary Structure 
PELPEVEVTRRGLLPHVVGRRIADVTVRHRGLRWPVEDDLEARLAGRLVRRIERRGKYL
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEE
LLECVDEATDDAGWLLVHLGMTGTLRVLPDAPPAGAHDHLDLVLDDAGGSRIVLRFRDPR
EEEEHHCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEECCCH
RFGAVLWSPLSEAMLPGHPLLRGLGIEPFDSHFDGSWLHRHTRGRSAAIKTVLLAGNIVV
HHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHHHHHEEECCEEE
GVGNIYASESLFRAGIRPTTPAGRLSLARCERLAQSVRETLAQAIERGGSTLRDFVGSDG
EECCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCC
ASGYFQLECFVYDRAGEPCKVCGTPVRQIVQGQRSTFYCTHCQH
CCCEEEEEEEEEECCCCCHHHCCHHHHHHHCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA