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The map label for this gene is rpoH [H]

Identifier: 73540031

GI number: 73540031

Start: 354402

End: 355331

Strand: Reverse

Name: rpoH [H]

Synonym: Reut_A0325

Alternate gene names: 73540031

Gene position: 355331-354402 (Counterclockwise)

Preceding gene: 73540033

Following gene: 73540025

Centisome position: 9.33

GC content: 61.72

Gene sequence:

>930_bases
GTGAACGCAGTCTTGTCTGCCGACCAAACCCTGATGAACAGCCGTCTGCCGACGGCGCCCAAGCAGGCCGGAAGCTTTGC
ATTGACCTTCCCGGCCACTGTGGGCAACCTGGAAAGCTATATCCAGGCCGTCCATCGCATTCCTATGCTGACCGCAGAGG
AAGAGCAACGCCTCGCGCGCGAATTGCGCGAAGATGATTCCGTCGATGCCGCACGCCAGCTGGTGATGTCCCACCTGCGC
CTGGTGGTGTCGATCGCACGCCAGTACCTCGGGTACGGCCTCCCGCATGCCGACCTGATTCAGGAAGGCAATATCGGACT
GATGAAGGCCGTGAAGCGCTTCGACCCGAATCAGGGCGTGCGCCTGGTGTCGTATGCGATGCACTGGATCAAGGCCGAGA
TCCACGAGTACGTGCTGAAGAACTGGCGCATGGTCAAGGTGGCGACCACCAAGGCCCAGCGCAAGCTGTTCTTCAACCTG
CGCAGCCACAAACAGGGTTCCCATACCTTCACGCCGGAGCAGATCGAAGCCGTTGCGCGCGATCTCAACGTCAAGCCGGA
AGAGGTGATGGAGATGGAAACCCGCATGTCGGGTGGCGATCTCGCGTTGGAAGGCCAGGTTGATGACGGCGAGGAGGAAT
TCGCGCCGATCGCCTATCTGGCGGACAACCACAACGAGCCCACGCGCGTGTTGGAAGCCAAGCGCCAGGACCGTATGCAG
GTCGAGGGGCTCGAAGAGGCGCTGGCGCGACTGGATGAGCGCAGCCGCCGGATTATCGAGGCACGCTGGCTCAACGTTGA
GGATGACGGCTCGGGCGGTGCGACGCTGCACGAACTGGCTGACGAATTCGGTGTGTCGGCCGAGCGTATTCGCCAGATCG
AGGTAGCGGCGATGAAGAAGATGAAGGGGGCGTTGCAGGCGTTTGCCTGA

Upstream 100 bases:

>100_bases
CACTCACCCGGCTTGAGTGCTAAGATGTGTTGCAATGCCGTGCCCTCCCACCGGAGCGGCGCGCCGGCAACCGAACGCCG
GATGCGAAAGGAGCTACTGA

Downstream 100 bases:

>100_bases
CCCCCGGCGCTAACGAGGCGCCATGCAAAAGGCCGGTCCAGTGGACCGGCCTTTTTTCATTGCCGTGCCGTCTCTCAGGA
CAGCAACTGCTTGATATCGT

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MNAVLSADQTLMNSRLPTAPKQAGSFALTFPATVGNLESYIQAVHRIPMLTAEEEQRLARELREDDSVDAARQLVMSHLR
LVVSIARQYLGYGLPHADLIQEGNIGLMKAVKRFDPNQGVRLVSYAMHWIKAEIHEYVLKNWRMVKVATTKAQRKLFFNL
RSHKQGSHTFTPEQIEAVARDLNVKPEEVMEMETRMSGGDLALEGQVDDGEEEFAPIAYLADNHNEPTRVLEAKRQDRMQ
VEGLEEALARLDERSRRIIEARWLNVEDDGSGGATLHELADEFGVSAERIRQIEVAAMKKMKGALQAFA

Sequences:

>Translated_309_residues
MNAVLSADQTLMNSRLPTAPKQAGSFALTFPATVGNLESYIQAVHRIPMLTAEEEQRLARELREDDSVDAARQLVMSHLR
LVVSIARQYLGYGLPHADLIQEGNIGLMKAVKRFDPNQGVRLVSYAMHWIKAEIHEYVLKNWRMVKVATTKAQRKLFFNL
RSHKQGSHTFTPEQIEAVARDLNVKPEEVMEMETRMSGGDLALEGQVDDGEEEFAPIAYLADNHNEPTRVLEAKRQDRMQ
VEGLEEALARLDERSRRIIEARWLNVEDDGSGGATLHELADEFGVSAERIRQIEVAAMKKMKGALQAFA
>Mature_309_residues
MNAVLSADQTLMNSRLPTAPKQAGSFALTFPATVGNLESYIQAVHRIPMLTAEEEQRLARELREDDSVDAARQLVMSHLR
LVVSIARQYLGYGLPHADLIQEGNIGLMKAVKRFDPNQGVRLVSYAMHWIKAEIHEYVLKNWRMVKVATTKAQRKLFFNL
RSHKQGSHTFTPEQIEAVARDLNVKPEEVMEMETRMSGGDLALEGQVDDGEEEFAPIAYLADNHNEPTRVLEAKRQDRMQ
VEGLEEALARLDERSRRIIEARWLNVEDDGSGGATLHELADEFGVSAERIRQIEVAAMKKMKGALQAFA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=278, Percent_Identity=55.3956834532374, Blast_Score=289, Evalue=1e-79,
Organism=Escherichia coli, GI1789098, Length=274, Percent_Identity=32.8467153284672, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1789448, Length=240, Percent_Identity=30, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34818; Mature: 34818

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAVLSADQTLMNSRLPTAPKQAGSFALTFPATVGNLESYIQAVHRIPMLTAEEEQRLAR
CCCCHHHHHHHHHCCCCCCCCCCCCEEEECCHHHCCHHHHHHHHHHCCCCCCHHHHHHHH
ELREDDSVDAARQLVMSHLRLVVSIARQYLGYGLPHADLIQEGNIGLMKAVKRFDPNQGV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHCCCCCCH
RLVSYAMHWIKAEIHEYVLKNWRMVKVATTKAQRKLFFNLRSHKQGSHTFTPEQIEAVAR
HHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
DLNVKPEEVMEMETRMSGGDLALEGQVDDGEEEFAPIAYLADNHNEPTRVLEAKRQDRMQ
HCCCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCCCEEECCCCCHHHHHHHHHHHHHH
VEGLEEALARLDERSRRIIEARWLNVEDDGSGGATLHELADEFGVSAERIRQIEVAAMKK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
MKGALQAFA
HHHHHHHCC
>Mature Secondary Structure
MNAVLSADQTLMNSRLPTAPKQAGSFALTFPATVGNLESYIQAVHRIPMLTAEEEQRLAR
CCCCHHHHHHHHHCCCCCCCCCCCCEEEECCHHHCCHHHHHHHHHHCCCCCCHHHHHHHH
ELREDDSVDAARQLVMSHLRLVVSIARQYLGYGLPHADLIQEGNIGLMKAVKRFDPNQGV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHCCCCCCH
RLVSYAMHWIKAEIHEYVLKNWRMVKVATTKAQRKLFFNLRSHKQGSHTFTPEQIEAVAR
HHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
DLNVKPEEVMEMETRMSGGDLALEGQVDDGEEEFAPIAYLADNHNEPTRVLEAKRQDRMQ
HCCCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCCCEEECCCCCHHHHHHHHHHHHHH
VEGLEEALARLDERSRRIIEARWLNVEDDGSGGATLHELADEFGVSAERIRQIEVAAMKK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
MKGALQAFA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]