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The map label for this gene is gpsA

Identifier: 73540014

GI number: 73540014

Start: 339360

End: 340403

Strand: Direct

Name: gpsA

Synonym: Reut_A0308

Alternate gene names: 73540014

Gene position: 339360-340403 (Clockwise)

Preceding gene: 73540013

Following gene: 73540015

Centisome position: 8.92

GC content: 69.83

Gene sequence:

>1044_bases
TTGCGCCGATCCACCGTTCGAGCCGCCATGAAACTGACCTTCCTCGGTGCAGGTGCCTGGGGCACCGCACTTGCGAGCCA
TGCCGCCGCCAACCATGACGTGGTGCTGTGGGGACGCGATCCCGCCCAGCTTGCCGCGATCGCGGCGACCGGCAGCAACG
AGGCCTATCTGCCGGGCGTGCCACTGTCTCCGCGGCTGCGCGTGGAAGCGGACTTCGAGCAGGCAGTCGGTCATGCCGCC
GACGATCCCGATGGCCTCGTAGTCGTAGCCACGCCCGTGTCGGGCCTGCGCGAAATGACGCGCCGGCTCGCCGGGCGCGG
CAATGGCCATGTGCGCATGCTGTGGCTCTGCAAGGGCTTCGAGGCCGGCACGCACGCGCTGCCGCACCAGATGGTGCGCG
AAGAACTCGACGCCGCGGGCCGCACCAGCGGATTCGACTATGGCGTCCTGACCGGCCCCAGCTTTGCGCGCGAGGTGGCG
CTGGGCCTGCCGTGCGCGCTGACAGTCGCGGGCAGCGTGCCAACGCTGGCGGATTGCGCGCAGGCGGCATTCCATCATCA
CGCCATGCGCATCTACGGCAGCGACGACCTGACCGGTGTCGAGGTCGGCGGCGCAGTGAAGAACGTGCTGGCGATCGCCA
CCGGTGCGAGCGATGGCCTGGGTCTGGGGCTCAACGCGCGCGCCGCGCTGGTGACGCGTGGGCTGGCCGAAATGACGCGC
CTTGGCCTGGCGCTTGGTGGACGCGCGGAAACGTTCATGGGCCTCGCCGGCGTGGGCGACCTGATCCTGACGGCTACCGG
TGACCTGTCGCGTAATCGCAAGGTCGGACAGCAGCTCGCGGCCGGACAAAGCCTCGATCAGATCCTCGCGAGCCTTGGGC
ATGTCGCCGAGGGCGTGCGTTGCGCGCAGGCCGTGGCCGCACTGGCCGAGGCCTGCAAGATCGAGATGCCGATCACGCGC
GCCGTGTGCGCGGTGTTGTTCGAAGGCCTGAGCGCCGCGGATGCCGTGGCGCAACTCCTGCAGCGCGACGCGCGCGACGA
ATAG

Upstream 100 bases:

>100_bases
GCCAGGCTCGCCACTGATCCGGCAGTCCCGCACTGCCTGACGAAACGGGGCTGCGGCCCCGTTTTGCCTTTTCGGGGCCC
GCGCACAGGGCTACCATCGA

Downstream 100 bases:

>100_bases
CCTTCGTTCAGTACTCTTCGATTCCCTACGCATGACTTCCCGTCGCCGCGCGCTGATCGCGCTTGCCTGTACCTGCGCTG
CCGGCGCAGTTTCCCCTGCC

Product: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MRRSTVRAAMKLTFLGAGAWGTALASHAAANHDVVLWGRDPAQLAAIAATGSNEAYLPGVPLSPRLRVEADFEQAVGHAA
DDPDGLVVVATPVSGLREMTRRLAGRGNGHVRMLWLCKGFEAGTHALPHQMVREELDAAGRTSGFDYGVLTGPSFAREVA
LGLPCALTVAGSVPTLADCAQAAFHHHAMRIYGSDDLTGVEVGGAVKNVLAIATGASDGLGLGLNARAALVTRGLAEMTR
LGLALGGRAETFMGLAGVGDLILTATGDLSRNRKVGQQLAAGQSLDQILASLGHVAEGVRCAQAVAALAEACKIEMPITR
AVCAVLFEGLSAADAVAQLLQRDARDE

Sequences:

>Translated_347_residues
MRRSTVRAAMKLTFLGAGAWGTALASHAAANHDVVLWGRDPAQLAAIAATGSNEAYLPGVPLSPRLRVEADFEQAVGHAA
DDPDGLVVVATPVSGLREMTRRLAGRGNGHVRMLWLCKGFEAGTHALPHQMVREELDAAGRTSGFDYGVLTGPSFAREVA
LGLPCALTVAGSVPTLADCAQAAFHHHAMRIYGSDDLTGVEVGGAVKNVLAIATGASDGLGLGLNARAALVTRGLAEMTR
LGLALGGRAETFMGLAGVGDLILTATGDLSRNRKVGQQLAAGQSLDQILASLGHVAEGVRCAQAVAALAEACKIEMPITR
AVCAVLFEGLSAADAVAQLLQRDARDE
>Mature_347_residues
MRRSTVRAAMKLTFLGAGAWGTALASHAAANHDVVLWGRDPAQLAAIAATGSNEAYLPGVPLSPRLRVEADFEQAVGHAA
DDPDGLVVVATPVSGLREMTRRLAGRGNGHVRMLWLCKGFEAGTHALPHQMVREELDAAGRTSGFDYGVLTGPSFAREVA
LGLPCALTVAGSVPTLADCAQAAFHHHAMRIYGSDDLTGVEVGGAVKNVLAIATGASDGLGLGLNARAALVTRGLAEMTR
LGLALGGRAETFMGLAGVGDLILTATGDLSRNRKVGQQLAAGQSLDQILASLGHVAEGVRCAQAVAALAEACKIEMPITR
AVCAVLFEGLSAADAVAQLLQRDARDE

Specific function: De novo phospholipid biosynthesis; glycerol-3 phosphate formation. [C]

COG id: COG0240

COG function: function code C; Glycerol-3-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI33695088, Length=350, Percent_Identity=29.1428571428571, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI24307999, Length=302, Percent_Identity=28.476821192053, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1790037, Length=338, Percent_Identity=43.491124260355, Blast_Score=246, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17507425, Length=342, Percent_Identity=27.1929824561404, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI32564403, Length=327, Percent_Identity=27.5229357798165, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32564399, Length=319, Percent_Identity=27.8996865203762, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI193210136, Length=327, Percent_Identity=27.5229357798165, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI193210134, Length=186, Percent_Identity=33.8709677419355, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324513, Length=374, Percent_Identity=29.4117647058824, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6320181, Length=371, Percent_Identity=29.3800539083558, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17136200, Length=353, Percent_Identity=25.4957507082153, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17136204, Length=354, Percent_Identity=25.7062146892655, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17136202, Length=354, Percent_Identity=25.7062146892655, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI22026922, Length=300, Percent_Identity=23.6666666666667, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45551945, Length=339, Percent_Identity=25.9587020648968, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281362270, Length=325, Percent_Identity=26.1538461538462, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648969, Length=295, Percent_Identity=26.1016949152542, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPDA_CUPPJ (Q476J3)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294534.1
- ProteinModelPortal:   Q476J3
- SMR:   Q476J3
- GeneID:   3609853
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0308
- NMPDR:   fig|264198.3.peg.820
- HOGENOM:   HBG586392
- OMA:   ALVACCK
- ProtClustDB:   PRK00094
- BioCyc:   REUT264198:REUT_A0308-MONOMER
- HAMAP:   MF_00394
- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR006168
- InterPro:   IPR006109
- InterPro:   IPR011128
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR11728
- PIRSF:   PIRSF000114
- PRINTS:   PR00077

Pfam domain/function: PF07479 NAD_Gly3P_dh_C; PF01210 NAD_Gly3P_dh_N; SSF48179 6DGDH_C_like

EC number: =1.1.1.94

Molecular weight: Translated: 35750; Mature: 35750

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00957 NAD_G3PDH

Important sites: ACT_SITE 207-207 BINDING 118-118 BINDING 118-118 BINDING 156-156 BINDING 271-271 BINDING 297-297

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRSTVRAAMKLTFLGAGAWGTALASHAAANHDVVLWGRDPAQLAAIAATGSNEAYLPGV
CCCHHHHHHHHHHEEECCHHHHHHHHHHCCCCCEEEECCCHHHEEEEEECCCCCCCCCCC
PLSPRLRVEADFEQAVGHAADDPDGLVVVATPVSGLREMTRRLAGRGNGHVRMLWLCKGF
CCCCCEEEEECHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEEEECCC
EAGTHALPHQMVREELDAAGRTSGFDYGVLTGPSFAREVALGLPCALTVAGSVPTLADCA
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHH
QAAFHHHAMRIYGSDDLTGVEVGGAVKNVLAIATGASDGLGLGLNARAALVTRGLAEMTR
HHHHHHHHEEEECCCCCCCEEHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHH
LGLALGGRAETFMGLAGVGDLILTATGDLSRNRKVGQQLAAGQSLDQILASLGHVAEGVR
HHHHCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
CAQAVAALAEACKIEMPITRAVCAVLFEGLSAADAVAQLLQRDARDE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRRSTVRAAMKLTFLGAGAWGTALASHAAANHDVVLWGRDPAQLAAIAATGSNEAYLPGV
CCCHHHHHHHHHHEEECCHHHHHHHHHHCCCCCEEEECCCHHHEEEEEECCCCCCCCCCC
PLSPRLRVEADFEQAVGHAADDPDGLVVVATPVSGLREMTRRLAGRGNGHVRMLWLCKGF
CCCCCEEEEECHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEEEECCC
EAGTHALPHQMVREELDAAGRTSGFDYGVLTGPSFAREVALGLPCALTVAGSVPTLADCA
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHH
QAAFHHHAMRIYGSDDLTGVEVGGAVKNVLAIATGASDGLGLGLNARAALVTRGLAEMTR
HHHHHHHHEEEECCCCCCCEEHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHH
LGLALGGRAETFMGLAGVGDLILTATGDLSRNRKVGQQLAAGQSLDQILASLGHVAEGVR
HHHHCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
CAQAVAALAEACKIEMPITRAVCAVLFEGLSAADAVAQLLQRDARDE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA