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The map label for this gene is phbI [H]

Identifier: 73540003

GI number: 73540003

Start: 329153

End: 330913

Strand: Direct

Name: phbI [H]

Synonym: Reut_A0297

Alternate gene names: 73540003

Gene position: 329153-330913 (Clockwise)

Preceding gene: 73540002

Following gene: 73540004

Centisome position: 8.65

GC content: 66.33

Gene sequence:

>1761_bases
ATGTCATTTGCCCTGCACGGCATCCCGGTCTCGCGCGGCGTCGCCATCGGCCGCGCGCATCTTCTTGCTCCAGCCGCGCT
GGACGTCTCGCACTACCTGGTCGATGAAGACCGGCTCGACGACGAAGTCGAACGACTTCGATCCGCACGTGCCGCAGTAC
GTGCGGAACTCATCACGCTGAAGCGCGACCTCCCGCGTGACGCCCCCGAGGAACTCGGCGCGTTCCTTGACGTGCATGCC
ATGATCCTCGACGACGAGGCGCTGTCGCGCGAGCCCGAGGCACTGATTCGCGGACGCCGCTACAACGCGGAGTGGGCGCT
GACCACGCGGCTCGAAGAGCTGATGCGCCAGTTCGACGAGATCGAAGATGAATACCTGCGCGAGCGCAAGACCGATATCC
ACCAAGTCGTCGAACGGATCCTGAAGGTGCTGGCCGGTGCGCCGGTGCTGGCGCCGGCCCCGGTTCCCGCGCTGGCTGCG
GACGGCGAGCCGGCGCCGGGCGTGATCGTCGTCGCGCACGACATTGCGCCGGCCGACATGCTGCAGTTCCGGCACACGGT
CTTCCACGGGTTCGTCACCGACATGGGAGGGAAGACGTCGCATACCGCCATCGTCGCGCGTAGCCTGGACATTCCGGCCG
CGGTCGGTGTGCAAAGCGCCAGCGAGCTGATCCGCCAGGACGACTGGATCATCGTCGACGGCGATGCCGGTCTCGTGATC
GTCGATCCGTCGGCCATCATCCTCGAGGAATACCGCCACCGCCAGAGCGAGCGCGCGCTGGAAAAGAAGCGCCTGCAGCG
CCTTCGCCATACGCCGGCGGTCACGCTGGACGGCCAGGAAATCGACCTGCTGGCTAATATCGAGATGGCCGAGGACGCGG
GCGTAGCGCTGTCGGCAGGCGCCGTGGGCGTGGGCCTGTTCCGTTCGGAATTCCTGTTCATGAACCGTCGCGGCGAACTG
CCGGACGAGGATGAGCAATTCGCGGCCTATCGCGGTGCGGTCGAAGCCATGCACGGCTTGCCGGTGACGATCCGCACGAT
CGACATCGGCGCCGACAAGCCGCTGGATGGCCGCGGCGACGATTTTGAGACCGCACTGAATCCTGCGCTCGGACTACGTG
CGATCCGCTGGTCGCTGTCCGAGCCGGCCATGTTCCTGACCCAGTTGCGTGCGCTGCTGCGCGCGTCGGCGTTCGGGCCG
GTACGGCTGCTCGTGCCAATGCTCTCGCATGCGCGTGAGATCGACCAGACACTGGAACTGCTCGCACAGGCCAAGCGCCA
GCTCGACGAGCGCGGCGTGCCTTACGATCCCGGCCTGAAGGTCGGCGCCATGATCGAGATTCCGGCCGCTGTGCTGCTGT
TGCCGCTGTTCCTGCGCCGGATGGATTTCCTGTCGATCGGCACCAACGACCTCACGCAGTACACGCTGGCCATCGACCGC
GCCGATAACGCCGTGGCGCATCTGTTCGATCCGATGCACCCGGCCGTGCTGCAGCTCATTGCGCGCACCATCCGCGAAGC
CAATCGTGCCGGCGTACCGGTGGCAGTGTGCGGCGAGATGGCCGGGGATCCGAGCATGACGCGGCTGCTTCTGGGCATGG
GCCTGCGTGAGTTCTCGATGCATCCGGCCCAACTGCTGCGGGTCAAGCAAGAAGTCCTGCACGCCGATTGCGCGCGGCTG
GAGCCGCTCGTGGACCAGGTCCTCAACGCCTATGACCCCCTGGAACAGACGGCGGCGCTGGAACAGCTCGCTAAGCCCTA
A

Upstream 100 bases:

>100_bases
ATGGATGCGCTGCTGGCGCTGATCGCCAACCGCTTTGGCGAGGGCGAGTGAGGCCCGCCGCGCTGCCATGGTTTCCTGAA
CGTTCCTGACGGAGCGGCAC

Downstream 100 bases:

>100_bases
AATCTGAAGGGTTTTTTTGCTCGCCACCGGCGCATAAACCATCACTTTGTTGATGGCGATGTGCGCCGGAATCGGCTTCT
CCCTTCTCCTTGAGTGACGC

Product: phosphoenolpyruvate--protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I; Protein I [H]

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MSFALHGIPVSRGVAIGRAHLLAPAALDVSHYLVDEDRLDDEVERLRSARAAVRAELITLKRDLPRDAPEELGAFLDVHA
MILDDEALSREPEALIRGRRYNAEWALTTRLEELMRQFDEIEDEYLRERKTDIHQVVERILKVLAGAPVLAPAPVPALAA
DGEPAPGVIVVAHDIAPADMLQFRHTVFHGFVTDMGGKTSHTAIVARSLDIPAAVGVQSASELIRQDDWIIVDGDAGLVI
VDPSAIILEEYRHRQSERALEKKRLQRLRHTPAVTLDGQEIDLLANIEMAEDAGVALSAGAVGVGLFRSEFLFMNRRGEL
PDEDEQFAAYRGAVEAMHGLPVTIRTIDIGADKPLDGRGDDFETALNPALGLRAIRWSLSEPAMFLTQLRALLRASAFGP
VRLLVPMLSHAREIDQTLELLAQAKRQLDERGVPYDPGLKVGAMIEIPAAVLLLPLFLRRMDFLSIGTNDLTQYTLAIDR
ADNAVAHLFDPMHPAVLQLIARTIREANRAGVPVAVCGEMAGDPSMTRLLLGMGLREFSMHPAQLLRVKQEVLHADCARL
EPLVDQVLNAYDPLEQTAALEQLAKP

Sequences:

>Translated_586_residues
MSFALHGIPVSRGVAIGRAHLLAPAALDVSHYLVDEDRLDDEVERLRSARAAVRAELITLKRDLPRDAPEELGAFLDVHA
MILDDEALSREPEALIRGRRYNAEWALTTRLEELMRQFDEIEDEYLRERKTDIHQVVERILKVLAGAPVLAPAPVPALAA
DGEPAPGVIVVAHDIAPADMLQFRHTVFHGFVTDMGGKTSHTAIVARSLDIPAAVGVQSASELIRQDDWIIVDGDAGLVI
VDPSAIILEEYRHRQSERALEKKRLQRLRHTPAVTLDGQEIDLLANIEMAEDAGVALSAGAVGVGLFRSEFLFMNRRGEL
PDEDEQFAAYRGAVEAMHGLPVTIRTIDIGADKPLDGRGDDFETALNPALGLRAIRWSLSEPAMFLTQLRALLRASAFGP
VRLLVPMLSHAREIDQTLELLAQAKRQLDERGVPYDPGLKVGAMIEIPAAVLLLPLFLRRMDFLSIGTNDLTQYTLAIDR
ADNAVAHLFDPMHPAVLQLIARTIREANRAGVPVAVCGEMAGDPSMTRLLLGMGLREFSMHPAQLLRVKQEVLHADCARL
EPLVDQVLNAYDPLEQTAALEQLAKP
>Mature_585_residues
SFALHGIPVSRGVAIGRAHLLAPAALDVSHYLVDEDRLDDEVERLRSARAAVRAELITLKRDLPRDAPEELGAFLDVHAM
ILDDEALSREPEALIRGRRYNAEWALTTRLEELMRQFDEIEDEYLRERKTDIHQVVERILKVLAGAPVLAPAPVPALAAD
GEPAPGVIVVAHDIAPADMLQFRHTVFHGFVTDMGGKTSHTAIVARSLDIPAAVGVQSASELIRQDDWIIVDGDAGLVIV
DPSAIILEEYRHRQSERALEKKRLQRLRHTPAVTLDGQEIDLLANIEMAEDAGVALSAGAVGVGLFRSEFLFMNRRGELP
DEDEQFAAYRGAVEAMHGLPVTIRTIDIGADKPLDGRGDDFETALNPALGLRAIRWSLSEPAMFLTQLRALLRASAFGPV
RLLVPMLSHAREIDQTLELLAQAKRQLDERGVPYDPGLKVGAMIEIPAAVLLLPLFLRRMDFLSIGTNDLTQYTLAIDRA
DNAVAHLFDPMHPAVLQLIARTIREANRAGVPVAVCGEMAGDPSMTRLLLGMGLREFSMHPAQLLRVKQEVLHADCARLE
PLVDQVLNAYDPLEQTAALEQLAKP

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=564, Percent_Identity=38.8297872340425, Blast_Score=380, Evalue=1e-106,
Organism=Escherichia coli, GI1789193, Length=570, Percent_Identity=34.0350877192982, Blast_Score=286, Evalue=4e-78,
Organism=Escherichia coli, GI48994992, Length=519, Percent_Identity=32.7552986512524, Blast_Score=261, Evalue=8e-71,
Organism=Escherichia coli, GI1788726, Length=579, Percent_Identity=30.3972366148532, Blast_Score=224, Evalue=9e-60,
Organism=Escherichia coli, GI1787994, Length=399, Percent_Identity=27.0676691729323, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI226510935, Length=173, Percent_Identity=27.7456647398844, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 64452; Mature: 64321

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFALHGIPVSRGVAIGRAHLLAPAALDVSHYLVDEDRLDDEVERLRSARAAVRAELITL
CCCEECCCCHHCCCHHHHHHHHCHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHH
KRDLPRDAPEELGAFLDVHAMILDDEALSREPEALIRGRRYNAEWALTTRLEELMRQFDE
HHHCCCCCHHHHHHHHHHHHHEECCHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHH
IEDEYLRERKTDIHQVVERILKVLAGAPVLAPAPVPALAADGEPAPGVIVVAHDIAPADM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHH
LQFRHTVFHGFVTDMGGKTSHTAIVARSLDIPAAVGVQSASELIRQDDWIIVDGDAGLVI
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEE
VDPSAIILEEYRHRQSERALEKKRLQRLRHTPAVTLDGQEIDLLANIEMAEDAGVALSAG
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCEECCCCCCEEECC
AVGVGLFRSEFLFMNRRGELPDEDEQFAAYRGAVEAMHGLPVTIRTIDIGADKPLDGRGD
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCC
DFETALNPALGLRAIRWSLSEPAMFLTQLRALLRASAFGPVRLLVPMLSHAREIDQTLEL
CHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LAQAKRQLDERGVPYDPGLKVGAMIEIPAAVLLLPLFLRRMDFLSIGTNDLTQYTLAIDR
HHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEEC
ADNAVAHLFDPMHPAVLQLIARTIREANRAGVPVAVCGEMAGDPSMTRLLLGMGLREFSM
CCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHCCHHHHCC
HPAQLLRVKQEVLHADCARLEPLVDQVLNAYDPLEQTAALEQLAKP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SFALHGIPVSRGVAIGRAHLLAPAALDVSHYLVDEDRLDDEVERLRSARAAVRAELITL
CCEECCCCHHCCCHHHHHHHHCHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHH
KRDLPRDAPEELGAFLDVHAMILDDEALSREPEALIRGRRYNAEWALTTRLEELMRQFDE
HHHCCCCCHHHHHHHHHHHHHEECCHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHH
IEDEYLRERKTDIHQVVERILKVLAGAPVLAPAPVPALAADGEPAPGVIVVAHDIAPADM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHH
LQFRHTVFHGFVTDMGGKTSHTAIVARSLDIPAAVGVQSASELIRQDDWIIVDGDAGLVI
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEE
VDPSAIILEEYRHRQSERALEKKRLQRLRHTPAVTLDGQEIDLLANIEMAEDAGVALSAG
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCEECCCCCCEEECC
AVGVGLFRSEFLFMNRRGELPDEDEQFAAYRGAVEAMHGLPVTIRTIDIGADKPLDGRGD
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCC
DFETALNPALGLRAIRWSLSEPAMFLTQLRALLRASAFGPVRLLVPMLSHAREIDQTLEL
CHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LAQAKRQLDERGVPYDPGLKVGAMIEIPAAVLLLPLFLRRMDFLSIGTNDLTQYTLAIDR
HHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEEC
ADNAVAHLFDPMHPAVLQLIARTIREANRAGVPVAVCGEMAGDPSMTRLLLGMGLREFSM
CCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHCCHHHHCC
HPAQLLRVKQEVLHADCARLEPLVDQVLNAYDPLEQTAALEQLAKP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653223 [H]