Click here to switch to the map view.

The map label for this gene is braE [H]

Identifier: 73539969

GI number: 73539969

Start: 292649

End: 293725

Strand: Direct

Name: braE [H]

Synonym: Reut_A0263

Alternate gene names: 73539969

Gene position: 292649-293725 (Clockwise)

Preceding gene: 73539968

Following gene: 73539970

Centisome position: 7.69

GC content: 62.86

Gene sequence:

>1077_bases
ATGTTCTATCGTGAAGCCGGCCAGTTCAAGACGAGCTACGTCTCGGACAGCCAGATCTTCCCCATCCGTCAGGACCGCAT
CGGCTTTGCGGTGCTGATGGCGGTGGCCTTCGGCGTGATTCCGTTCGTTGGGTCGGAATACTGGTTCTCGGCCATCCTGA
TTCCGTTCCTGATCTTCTCGCTCGCGGCGCTGGGGCTGAACATCCTCACGGGCTACGCAGGCCAACTGTCGCTTGGTACG
GCCGCTTTCATGGCGGTGGGTGCCTACGCCGCGTACAACTTCCAGCTTCGCATCGAGGGGATGCCGGTGCTGCTCACCTT
CATCCTCGCCGGCCTGTCGGCGGCGGTGGTGGGCGTGGCCTTCGGCTTGCCGTCGCTGCGCATCAAGGGCTTTTATCTCG
CGGTCGCCACGCTCGCCGCGCAGTTCTTCGTAGTGTGGGCGCTGACCAAGTTCCCCTGGTTCTCCAACAACAGCTCGTCG
GGCGTGATCACGGCGCAGCGGCTGGACCTGTTCGGCATCGCCATCGACACGCCGGTGAAGAAGTATCTGTTCGTGCTGGC
CCTCGTCACCGTACTGGCGTTCATCGCCAAGAACCTGGTGCGCTCGGCCACGGGCCGTGCATGGATGTCGGTGCGCGACA
TGGACGTGGCAGCGGAAGTCATCGGCATTCCGCTGATGCGCACCAAGCTGCTGGCCTTTGCCGTGAGCTCGTTCTACTGC
GGCGTGGCCGGCGCGCTGTATGCGTTCTGCTATCTCGGCTCGGTCGAGCCGGACGGCTTCTCGCTGGACCTGTCGTTCCG
CGTGTTGTTCATGATCATCATCGGCGGCGTGGGCAGCATCCTGGGTTCGTTCCTTGGCGCGGCGTTCATCCTGCTGCTGC
CGATCTTCCTGGACAACGTGCTGCCGCCGCTCGCATCGCTGCTGCACCTGCCGTTCACGAATGCCACGGTGTCGCATATC
CAGTTGATGGTGTTCGGTGGCCTGATCATCTTCTTCCTGATCGTCGAGCCGCACGGGCTTGCGCGGTTGTGGCAGATCTC
CAAGGAGAAACTCAGGCTGTGGCCGTTCCCGCACTGA

Upstream 100 bases:

>100_bases
ATGTGTTCGCGCTGCTCTTCCTGCTGGTCCGTCCGGAAGGGCTGTTCGGCGAGAAGCACATCGACCGCGTCTGAACTGAC
TACGCTCCAGGAGACTTTCC

Downstream 100 bases:

>100_bases
CCGTCGCACCGCTTATCGTTTTCTGACGTAGTTCGATCCAACGGGTACCCCATAACAAACGCACCTGCCAAGGTGAAGGA
GACTGTCATGACAACCCTGA

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MFYREAGQFKTSYVSDSQIFPIRQDRIGFAVLMAVAFGVIPFVGSEYWFSAILIPFLIFSLAALGLNILTGYAGQLSLGT
AAFMAVGAYAAYNFQLRIEGMPVLLTFILAGLSAAVVGVAFGLPSLRIKGFYLAVATLAAQFFVVWALTKFPWFSNNSSS
GVITAQRLDLFGIAIDTPVKKYLFVLALVTVLAFIAKNLVRSATGRAWMSVRDMDVAAEVIGIPLMRTKLLAFAVSSFYC
GVAGALYAFCYLGSVEPDGFSLDLSFRVLFMIIIGGVGSILGSFLGAAFILLLPIFLDNVLPPLASLLHLPFTNATVSHI
QLMVFGGLIIFFLIVEPHGLARLWQISKEKLRLWPFPH

Sequences:

>Translated_358_residues
MFYREAGQFKTSYVSDSQIFPIRQDRIGFAVLMAVAFGVIPFVGSEYWFSAILIPFLIFSLAALGLNILTGYAGQLSLGT
AAFMAVGAYAAYNFQLRIEGMPVLLTFILAGLSAAVVGVAFGLPSLRIKGFYLAVATLAAQFFVVWALTKFPWFSNNSSS
GVITAQRLDLFGIAIDTPVKKYLFVLALVTVLAFIAKNLVRSATGRAWMSVRDMDVAAEVIGIPLMRTKLLAFAVSSFYC
GVAGALYAFCYLGSVEPDGFSLDLSFRVLFMIIIGGVGSILGSFLGAAFILLLPIFLDNVLPPLASLLHLPFTNATVSHI
QLMVFGGLIIFFLIVEPHGLARLWQISKEKLRLWPFPH
>Mature_358_residues
MFYREAGQFKTSYVSDSQIFPIRQDRIGFAVLMAVAFGVIPFVGSEYWFSAILIPFLIFSLAALGLNILTGYAGQLSLGT
AAFMAVGAYAAYNFQLRIEGMPVLLTFILAGLSAAVVGVAFGLPSLRIKGFYLAVATLAAQFFVVWALTKFPWFSNNSSS
GVITAQRLDLFGIAIDTPVKKYLFVLALVTVLAFIAKNLVRSATGRAWMSVRDMDVAAEVIGIPLMRTKLLAFAVSSFYC
GVAGALYAFCYLGSVEPDGFSLDLSFRVLFMIIIGGVGSILGSFLGAAFILLLPIFLDNVLPPLASLLHLPFTNATVSHI
QLMVFGGLIIFFLIVEPHGLARLWQISKEKLRLWPFPH

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=312, Percent_Identity=27.2435897435897, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 38961; Mature: 38961

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYREAGQFKTSYVSDSQIFPIRQDRIGFAVLMAVAFGVIPFVGSEYWFSAILIPFLIFS
CCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LAALGLNILTGYAGQLSLGTAAFMAVGAYAAYNFQLRIEGMPVLLTFILAGLSAAVVGVA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHH
FGLPSLRIKGFYLAVATLAAQFFVVWALTKFPWFSNNSSSGVITAQRLDLFGIAIDTPVK
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHEEEEEECCHHH
KYLFVLALVTVLAFIAKNLVRSATGRAWMSVRDMDVAAEVIGIPLMRTKLLAFAVSSFYC
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GVAGALYAFCYLGSVEPDGFSLDLSFRVLFMIIIGGVGSILGSFLGAAFILLLPIFLDNV
HHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPPLASLLHLPFTNATVSHIQLMVFGGLIIFFLIVEPHGLARLWQISKEKLRLWPFPH
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFYREAGQFKTSYVSDSQIFPIRQDRIGFAVLMAVAFGVIPFVGSEYWFSAILIPFLIFS
CCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LAALGLNILTGYAGQLSLGTAAFMAVGAYAAYNFQLRIEGMPVLLTFILAGLSAAVVGVA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHH
FGLPSLRIKGFYLAVATLAAQFFVVWALTKFPWFSNNSSSGVITAQRLDLFGIAIDTPVK
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHEEEEEECCHHH
KYLFVLALVTVLAFIAKNLVRSATGRAWMSVRDMDVAAEVIGIPLMRTKLLAFAVSSFYC
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GVAGALYAFCYLGSVEPDGFSLDLSFRVLFMIIIGGVGSILGSFLGAAFILLLPIFLDNV
HHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPPLASLLHLPFTNATVSHIQLMVFGGLIIFFLIVEPHGLARLWQISKEKLRLWPFPH
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]