Click here to switch to the map view.

The map label for this gene is cca

Identifier: 73539928

GI number: 73539928

Start: 248266

End: 249513

Strand: Direct

Name: cca

Synonym: Reut_A0222

Alternate gene names: 73539928

Gene position: 248266-249513 (Clockwise)

Preceding gene: 73539927

Following gene: 73539931

Centisome position: 6.52

GC content: 68.19

Gene sequence:

>1248_bases
ATGCAGGTGTACGCCGTCGGCGGCGCGATCCGCGACGAACTGCTGGGCAAGCCCAGCCAGGACCGCGACTATGTCGTGGT
GGGGGCTACCCCGGCCGACATGGAGGCCGCCGGCTACAAGCCGGTCGGCAAGGACTTTCCGGTCTTCCTGCATCCGCGCA
CGAAAGAGGAATACGCGCTGGCGCGTACCGAGCGCAAGACCGCGATGGGCTACAAGGGTTTCGCGTTCTATTGCGAGCCT
GATGTCACGCTCGACGACGACCTGGTCCGGCGCGACCTGACCATCAACGCGATGGCGCGCGCCGTCGACGATGACGGCAA
GCTGGTTGGCCCCGTTATCGACCCGCACGGCGGCCAGCGCGACCTGGCGGCCCGGCAATTCCGGCATGTCTCCGAAGCGT
TCGCCGAAGATCCGGTGCGCATCCTGCGCGTTGCGCGTTTTGCCGCGCGCTTTCACGACTTCACCGTCGCGCCCGAGACC
GTTGCGCTGATGCGCAGGATGGTGGACGCCGGCGAAGTCGACGCGCTCGTGCCCGAGCGTGTCTGGCAGGAGCTTGCGCG
CGGCTTGATGGAGGCACGCCCGGCGCGCATGTTCGAGGTGCTACGCCAGTGCGGCGCGCTCGCGCGCCTGCTGCCCGAAC
TGGACCGGCTGTGGGGCGTGCCGCAGCGCGCGGACTACCACCCCGAAGTCGATACGGGCGTCCATGTGATGATGGTGATC
GATTGCGCCGCTGCGATGGGCACGACGCTCGCGGTGCGCTTCGCCGCGCTGGTCCATGACCTTGGCAAGGGCACCACGCC
CGAGCACGTGCTGCCGCGCCACCTGGGCCATGAAACGCGCAGCGTCGAACTGCTCGAAGACGTTTGCAAGCGCCTGCGCG
TGCCCAATGACTGCCGCGACCTGGCCGTGGTGGTGGCGCGCGAGCACGGCAACATCCATCGATCGATGGAATTCGGCGCG
GCCGCGATCACGCGCCTGCTCGAGCGCTGCGACGCGCTGCGCAAGCCGGGCCGCTTTGCCGACGCACTGCTCGCGTGCGA
GGCGGACAAGCGCGGGCGCAAGGGATTCGAGTCGGACACCTATCCGCAAAAGGCACGGCTGCTGGCAGCCCTGGATGCCG
CAGCCAGCGTGGACGCCGGCGCCATTGCCAAGGCGTTGGCCGACGATGTCAGCAAGATCAGGGAACGCGTGCACGAGGCA
CGCGTGGCAGCGGTGGATGCCCGATTAAAGCAGCTTGCCGATCACTAG

Upstream 100 bases:

>100_bases
GCTGACGACGAGCCGTATCGCCTGGCCCCGGACCGCGCCGATGCGATAATCATCAACCACTGAGCGCCGCGTCAGCGGCG
GAACACAGCGAGACAAGACA

Downstream 100 bases:

>100_bases
CGCGATCAGCGACAGCAGGATCGTCGACGCGAATGGCAGGGTGTAGTCGCGCCCGAACAGCCGGAAGCGCACGTCGCCGG
GCAACCGGCCGATGCCGAGC

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MQVYAVGGAIRDELLGKPSQDRDYVVVGATPADMEAAGYKPVGKDFPVFLHPRTKEEYALARTERKTAMGYKGFAFYCEP
DVTLDDDLVRRDLTINAMARAVDDDGKLVGPVIDPHGGQRDLAARQFRHVSEAFAEDPVRILRVARFAARFHDFTVAPET
VALMRRMVDAGEVDALVPERVWQELARGLMEARPARMFEVLRQCGALARLLPELDRLWGVPQRADYHPEVDTGVHVMMVI
DCAAAMGTTLAVRFAALVHDLGKGTTPEHVLPRHLGHETRSVELLEDVCKRLRVPNDCRDLAVVVAREHGNIHRSMEFGA
AAITRLLERCDALRKPGRFADALLACEADKRGRKGFESDTYPQKARLLAALDAAASVDAGAIAKALADDVSKIRERVHEA
RVAAVDARLKQLADH

Sequences:

>Translated_415_residues
MQVYAVGGAIRDELLGKPSQDRDYVVVGATPADMEAAGYKPVGKDFPVFLHPRTKEEYALARTERKTAMGYKGFAFYCEP
DVTLDDDLVRRDLTINAMARAVDDDGKLVGPVIDPHGGQRDLAARQFRHVSEAFAEDPVRILRVARFAARFHDFTVAPET
VALMRRMVDAGEVDALVPERVWQELARGLMEARPARMFEVLRQCGALARLLPELDRLWGVPQRADYHPEVDTGVHVMMVI
DCAAAMGTTLAVRFAALVHDLGKGTTPEHVLPRHLGHETRSVELLEDVCKRLRVPNDCRDLAVVVAREHGNIHRSMEFGA
AAITRLLERCDALRKPGRFADALLACEADKRGRKGFESDTYPQKARLLAALDAAASVDAGAIAKALADDVSKIRERVHEA
RVAAVDARLKQLADH
>Mature_415_residues
MQVYAVGGAIRDELLGKPSQDRDYVVVGATPADMEAAGYKPVGKDFPVFLHPRTKEEYALARTERKTAMGYKGFAFYCEP
DVTLDDDLVRRDLTINAMARAVDDDGKLVGPVIDPHGGQRDLAARQFRHVSEAFAEDPVRILRVARFAARFHDFTVAPET
VALMRRMVDAGEVDALVPERVWQELARGLMEARPARMFEVLRQCGALARLLPELDRLWGVPQRADYHPEVDTGVHVMMVI
DCAAAMGTTLAVRFAALVHDLGKGTTPEHVLPRHLGHETRSVELLEDVCKRLRVPNDCRDLAVVVAREHGNIHRSMEFGA
AAITRLLERCDALRKPGRFADALLACEADKRGRKGFESDTYPQKARLLAALDAAASVDAGAIAKALADDVSKIRERVHEA
RVAAVDARLKQLADH

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789436, Length=413, Percent_Identity=53.5108958837772, Blast_Score=417, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_CUPPJ (Q476S9)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294448.1
- ProteinModelPortal:   Q476S9
- SMR:   Q476S9
- GeneID:   3610342
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0222
- NMPDR:   fig|264198.3.peg.734
- HOGENOM:   HBG635063
- OMA:   RWACLLH
- ProtClustDB:   PRK10885
- BioCyc:   REUT264198:REUT_A0222-MONOMER
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 45862; Mature: 45862

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 143-143 BINDING 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVYAVGGAIRDELLGKPSQDRDYVVVGATPADMEAAGYKPVGKDFPVFLHPRTKEEYAL
CEEEEECCHHHHHHCCCCCCCCCEEEEECCCCCCHHCCCCCCCCCCCEEECCCCCHHHHH
ARTERKTAMGYKGFAFYCEPDVTLDDDLVRRDLTINAMARAVDDDGKLVGPVIDPHGGQR
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHCCHHHHHHHHHCCCCCEECCCCCCCCCCH
DLAARQFRHVSEAFAEDPVRILRVARFAARFHDFTVAPETVALMRRMVDAGEVDALVPER
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHH
VWQELARGLMEARPARMFEVLRQCGALARLLPELDRLWGVPQRADYHPEVDTGVHVMMVI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
DCAAAMGTTLAVRFAALVHDLGKGTTPEHVLPRHLGHETRSVELLEDVCKRLRVPNDCRD
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHH
LAVVVAREHGNIHRSMEFGAAAITRLLERCDALRKPGRFADALLACEADKRGRKGFESDT
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCCCCCCC
YPQKARLLAALDAAASVDAGAIAKALADDVSKIRERVHEARVAAVDARLKQLADH
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQVYAVGGAIRDELLGKPSQDRDYVVVGATPADMEAAGYKPVGKDFPVFLHPRTKEEYAL
CEEEEECCHHHHHHCCCCCCCCCEEEEECCCCCCHHCCCCCCCCCCCEEECCCCCHHHHH
ARTERKTAMGYKGFAFYCEPDVTLDDDLVRRDLTINAMARAVDDDGKLVGPVIDPHGGQR
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHCCHHHHHHHHHCCCCCEECCCCCCCCCCH
DLAARQFRHVSEAFAEDPVRILRVARFAARFHDFTVAPETVALMRRMVDAGEVDALVPER
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHH
VWQELARGLMEARPARMFEVLRQCGALARLLPELDRLWGVPQRADYHPEVDTGVHVMMVI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
DCAAAMGTTLAVRFAALVHDLGKGTTPEHVLPRHLGHETRSVELLEDVCKRLRVPNDCRD
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHH
LAVVVAREHGNIHRSMEFGAAAITRLLERCDALRKPGRFADALLACEADKRGRKGFESDT
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCCCCCCC
YPQKARLLAALDAAASVDAGAIAKALADDVSKIRERVHEARVAAVDARLKQLADH
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA