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The map label for this gene is ycdJ [C]

Identifier: 73539871

GI number: 73539871

Start: 192415

End: 193314

Strand: Direct

Name: ycdJ [C]

Synonym: Reut_A0165

Alternate gene names: 73539871

Gene position: 192415-193314 (Clockwise)

Preceding gene: 73539864

Following gene: 73539872

Centisome position: 5.05

GC content: 67.0

Gene sequence:

>900_bases
ATGCTGAACGATGCAAGCGCGCTGCTGTTCCCCAACTTCGAATCCTTCAGGCGCCGCGTGGGCGACATCGAGATCGCCGG
CGTGCAGGGCGGTTCCGGCCCGCCGCTGCTGCTCCTGCACGGGCATCCGCAGAGCCACCTGATCTGGCACAAGGTGGCGC
CCGTGCTGGCCGACCACTTCACCATCATCGCGACCGACCTGCGCGGTTATGGCAGCAGTTCGGCGCCGGCCGGCAACGCC
GGACACGAGAACTACAGCAAGCGCGCCATGGCGCAGGACCAGGTCGATGTCATGGCCGCATTTGGCTTCCATCGCTTTGC
GTTGTGCGGCCACGACCGCGGCGCGCGCGTGGCGCACCGCCTTGCCATGGACCATCCGCAAGCGGTCGAGCGGCTGATGC
TGCTCGATATCGCGCCGACGCTGGACATGTACGAGCGCACCAGCATGGCGTTTGCCGCGGCTTACTGGCACTGGTTCTTC
CTGATCCAGCCCACCCCCTTCCCCGAGACGCTGATCAACGCCGAGCCTGATTTCTATCTCAGCAAGCTGATGGGCCTGCG
CCACGCGGGCCTGACGCCGTTCTGTCCCGATGCCATGGCGGCCTATGGCGCGGCCATGCGTGAACCAGCGCGCGTGCACG
CCATGTGCGAGGACTATCGCGCGGCAGCTACCATCGATCTCGAACACGATCGCGCCGACCGCGATGCCGGGCGCAGGCTG
GAAATGCCGGTGCGCGTGCTGTGGGGCGAGCACGGCGTGGTGGCGCGCTGCTTCGAGCCGCTGGCGCTGTGGGAAGCCGT
CGGCCATAGCGTCAGCGGCAAGGCGCTGGACTGCGGACACTACATTCCGGAGGAATCGCCGCAGCCCCTGGCGCAAGAGA
TGCTCGCCTTCTTCCTCTAG

Upstream 100 bases:

>100_bases
GTCGCCCGCACAGCACACCGAGTGCGCGGTTTGCGCCTATGATCGGCAAGGAAGTACGTCGCCACCCACTCCTTCCAACG
GCCCAGACCGGAGACACGCG

Downstream 100 bases:

>100_bases
CCCTCCTGCCGTCAGGCCGCTTCCGTCGCATTACACCCCGATGCGCGCGGGTTAGCCCTGATTCCGTCTGGATGTCTGCA
ATTGCTAACATCAACGCCGC

Product: Alpha/beta hydrolase fold

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MLNDASALLFPNFESFRRRVGDIEIAGVQGGSGPPLLLLHGHPQSHLIWHKVAPVLADHFTIIATDLRGYGSSSAPAGNA
GHENYSKRAMAQDQVDVMAAFGFHRFALCGHDRGARVAHRLAMDHPQAVERLMLLDIAPTLDMYERTSMAFAAAYWHWFF
LIQPTPFPETLINAEPDFYLSKLMGLRHAGLTPFCPDAMAAYGAAMREPARVHAMCEDYRAAATIDLEHDRADRDAGRRL
EMPVRVLWGEHGVVARCFEPLALWEAVGHSVSGKALDCGHYIPEESPQPLAQEMLAFFL

Sequences:

>Translated_299_residues
MLNDASALLFPNFESFRRRVGDIEIAGVQGGSGPPLLLLHGHPQSHLIWHKVAPVLADHFTIIATDLRGYGSSSAPAGNA
GHENYSKRAMAQDQVDVMAAFGFHRFALCGHDRGARVAHRLAMDHPQAVERLMLLDIAPTLDMYERTSMAFAAAYWHWFF
LIQPTPFPETLINAEPDFYLSKLMGLRHAGLTPFCPDAMAAYGAAMREPARVHAMCEDYRAAATIDLEHDRADRDAGRRL
EMPVRVLWGEHGVVARCFEPLALWEAVGHSVSGKALDCGHYIPEESPQPLAQEMLAFFL
>Mature_299_residues
MLNDASALLFPNFESFRRRVGDIEIAGVQGGSGPPLLLLHGHPQSHLIWHKVAPVLADHFTIIATDLRGYGSSSAPAGNA
GHENYSKRAMAQDQVDVMAAFGFHRFALCGHDRGARVAHRLAMDHPQAVERLMLLDIAPTLDMYERTSMAFAAAYWHWFF
LIQPTPFPETLINAEPDFYLSKLMGLRHAGLTPFCPDAMAAYGAAMREPARVHAMCEDYRAAATIDLEHDRADRDAGRRL
EMPVRVLWGEHGVVARCFEPLALWEAVGHSVSGKALDCGHYIPEESPQPLAQEMLAFFL

Specific function: Catalyzes the hydrolytic defluorination of fluoroacetate to produce glycolate. Has low activity towards chloroacetate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI218777840, Length=276, Percent_Identity=27.8985507246377, Blast_Score=104, Evalue=9e-23,
Organism=Homo sapiens, GI13376164, Length=276, Percent_Identity=27.8985507246377, Blast_Score=104, Evalue=9e-23,
Organism=Homo sapiens, GI218777837, Length=232, Percent_Identity=24.5689655172414, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554012, Length=292, Percent_Identity=21.9178082191781, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.8.1.3 [H]

Molecular weight: Translated: 33138; Mature: 33138

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNDASALLFPNFESFRRRVGDIEIAGVQGGSGPPLLLLHGHPQSHLIWHKVAPVLADHF
CCCCCCEEECCCHHHHHHHHCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHCCC
TIIATDLRGYGSSSAPAGNAGHENYSKRAMAQDQVDVMAAFGFHRFALCGHDRGARVAHR
EEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHH
LAMDHPQAVERLMLLDIAPTLDMYERTSMAFAAAYWHWFFLIQPTPFPETLINAEPDFYL
HHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCHHHHCCCCHHHH
SKLMGLRHAGLTPFCPDAMAAYGAAMREPARVHAMCEDYRAAATIDLEHDRADRDAGRRL
HHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCEE
EMPVRVLWGEHGVVARCFEPLALWEAVGHSVSGKALDCGHYIPEESPQPLAQEMLAFFL
CCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHC
>Mature Secondary Structure
MLNDASALLFPNFESFRRRVGDIEIAGVQGGSGPPLLLLHGHPQSHLIWHKVAPVLADHF
CCCCCCEEECCCHHHHHHHHCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHCCC
TIIATDLRGYGSSSAPAGNAGHENYSKRAMAQDQVDVMAAFGFHRFALCGHDRGARVAHR
EEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHH
LAMDHPQAVERLMLLDIAPTLDMYERTSMAFAAAYWHWFFLIQPTPFPETLINAEPDFYL
HHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCHHHHCCCCHHHH
SKLMGLRHAGLTPFCPDAMAAYGAAMREPARVHAMCEDYRAAATIDLEHDRADRDAGRRL
HHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCEE
EMPVRVLWGEHGVVARCFEPLALWEAVGHSVSGKALDCGHYIPEESPQPLAQEMLAFFL
CCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA