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The map label for this gene is gatA [H]

Identifier: 73539780

GI number: 73539780

Start: 92182

End: 93687

Strand: Reverse

Name: gatA [H]

Synonym: Reut_A0074

Alternate gene names: 73539780

Gene position: 93687-92182 (Counterclockwise)

Preceding gene: 161611267

Following gene: 73539779

Centisome position: 2.46

GC content: 68.53

Gene sequence:

>1506_bases
ATGCCCTTTTCCGCTGATTCCGTGACTTCCCTGCGCCAGCTTGCCGACGCCCTGGCCGCGCGCTCCGTTTCCGCCGAGGA
ACTCGCGCGCACCTACCTGGCCCGCATCGAGCAGGCCGGCGCGCTGAACGCCTTCACCCATGTCGACGCCGAACGCACGC
TAGCCCAGGCCCGCGAAGCCGACGCACGCCGCGCGCGCGGCGAGGCCACGCTGCTCACCGGCGTGCCGGTGGCCCATAAG
GACGTGTTCGTGACGCGTGGCTGGCGCGCCACGGCCGGCTCGAAGATGCTGGCCAATTACGAAAGCCCGTTCGACGCCAC
CGTAGTCGAACGCATGGCCGCCGCCGGCATGGTGACGCTGGGCAAGACCAATATGGACGAGTTCGCGATGGGCTCGTCCA
ACGAAAACTCGTTCTTCGGCGCGGTGCGCAACCCGTGGGACACCGACCGTGTGCCGGGCGGCTCCTCGGGCGGCTCGGCC
GCCGCGGTGGCCGCGGGACTGGCGCCTGCCGCGACCGGCACCGACACCGGCGGTTCGATCCGCCAGCCGTCGTCGTTCTC
GGGCATCACCGGCATCAAGCCGACCTACGGCCGCGTATCGCGCTACGGCATGATCGCGTTCGCGTCGTCGCTGGACCAGG
GTGGCCCGATGGCCCACACCGCCGAAGACTGTGCGCTGCTGCTCAACGCCATGGCCGGTTTCGATGCGAAGGACTCGACC
AGCATCCCGCCCGAGCAAGGCGGCGTCGATGAAGACTTCGCGCGCTACCTGGGCCAGCCGCGCGCCGGCGCTTCCGAAAG
CCAGCCGCTGGCAGGCCTGCGCATCGGCCTGCCGCGCGAGTACTTCGGCAAGGGCCTGTCGCCCGATGTGGAAGAGACGG
TGCGCGCCGCGCTGCGCCAGTACGAACAACTCGGCGCCACGCTGGTCGAGGTCTCGCTGCCGAAGACCGAACTGTCGATT
CCCGTGTACTACGTAATCGCCCCGGCCGAAGCTTCATCGAACCTGTCACGCTTTGACGGCGTGCGCTACGGCCACCGCGC
GGCCGAGTACCGCGACCTGCTCGACATGTACAAGAAGTCGCGCGCCGAAGGCTTCGGCGCCGAGGTCAAGCGCCGCATCA
TGGTCGGCACCTACGTGCTGTCGCACGGCTACTACGACGCCTATTACCTGCAGGCGCAGAAGATCCGCCGCATCATCGCC
GACGACTTCCAGCGCGCGTTCGCCCAGTGCGACGTGATCATGGGCCCGGTCGCGCCCACGGTTGCATGGAAGCTCGGCGA
GAAGACCTCGGACCCGGTGCAGATGTACCTGGCCGACATCTTCACGCTGTCGACCAGCCTGGCGGGACTGCCCGGCATGA
GCGTGCCGTGCGGCTTCGGCGACGGCAATATGCCGGTCGGCCTGCAGCTGATCGGCAACTACTTCGACGAAGCGCGCCTG
CTGCAAACCGCGCACGCATTCCAGCAGGCGACCGACTGGCACCTGCGCCGTCCCGCCAAGGCATGA

Upstream 100 bases:

>100_bases
CAGAATGGCCTCTACCTGGTGCCCAAGGTCATCGAATGACCCCGTGCTGCCTCATTGCGCCCGCACCCAAGCACGCCACC
CCTGATGCCAACCGACTGTC

Downstream 100 bases:

>100_bases
AACGGCTTGCGCAAGGCTTCCGGCTGATCCTCGCTGCGGCGCTCGCGGCGGTGGTGGCCGGCTGCATCCAGTTGCCGATC
ATCGGCAAGCCGACGCCGAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MPFSADSVTSLRQLADALAARSVSAEELARTYLARIEQAGALNAFTHVDAERTLAQAREADARRARGEATLLTGVPVAHK
DVFVTRGWRATAGSKMLANYESPFDATVVERMAAAGMVTLGKTNMDEFAMGSSNENSFFGAVRNPWDTDRVPGGSSGGSA
AAVAAGLAPAATGTDTGGSIRQPSSFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMAHTAEDCALLLNAMAGFDAKDST
SIPPEQGGVDEDFARYLGQPRAGASESQPLAGLRIGLPREYFGKGLSPDVEETVRAALRQYEQLGATLVEVSLPKTELSI
PVYYVIAPAEASSNLSRFDGVRYGHRAAEYRDLLDMYKKSRAEGFGAEVKRRIMVGTYVLSHGYYDAYYLQAQKIRRIIA
DDFQRAFAQCDVIMGPVAPTVAWKLGEKTSDPVQMYLADIFTLSTSLAGLPGMSVPCGFGDGNMPVGLQLIGNYFDEARL
LQTAHAFQQATDWHLRRPAKA

Sequences:

>Translated_501_residues
MPFSADSVTSLRQLADALAARSVSAEELARTYLARIEQAGALNAFTHVDAERTLAQAREADARRARGEATLLTGVPVAHK
DVFVTRGWRATAGSKMLANYESPFDATVVERMAAAGMVTLGKTNMDEFAMGSSNENSFFGAVRNPWDTDRVPGGSSGGSA
AAVAAGLAPAATGTDTGGSIRQPSSFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMAHTAEDCALLLNAMAGFDAKDST
SIPPEQGGVDEDFARYLGQPRAGASESQPLAGLRIGLPREYFGKGLSPDVEETVRAALRQYEQLGATLVEVSLPKTELSI
PVYYVIAPAEASSNLSRFDGVRYGHRAAEYRDLLDMYKKSRAEGFGAEVKRRIMVGTYVLSHGYYDAYYLQAQKIRRIIA
DDFQRAFAQCDVIMGPVAPTVAWKLGEKTSDPVQMYLADIFTLSTSLAGLPGMSVPCGFGDGNMPVGLQLIGNYFDEARL
LQTAHAFQQATDWHLRRPAKA
>Mature_500_residues
PFSADSVTSLRQLADALAARSVSAEELARTYLARIEQAGALNAFTHVDAERTLAQAREADARRARGEATLLTGVPVAHKD
VFVTRGWRATAGSKMLANYESPFDATVVERMAAAGMVTLGKTNMDEFAMGSSNENSFFGAVRNPWDTDRVPGGSSGGSAA
AVAAGLAPAATGTDTGGSIRQPSSFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMAHTAEDCALLLNAMAGFDAKDSTS
IPPEQGGVDEDFARYLGQPRAGASESQPLAGLRIGLPREYFGKGLSPDVEETVRAALRQYEQLGATLVEVSLPKTELSIP
VYYVIAPAEASSNLSRFDGVRYGHRAAEYRDLLDMYKKSRAEGFGAEVKRRIMVGTYVLSHGYYDAYYLQAQKIRRIIAD
DFQRAFAQCDVIMGPVAPTVAWKLGEKTSDPVQMYLADIFTLSTSLAGLPGMSVPCGFGDGNMPVGLQLIGNYFDEARLL
QTAHAFQQATDWHLRRPAKA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=508, Percent_Identity=36.6141732283465, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI195972892, Length=500, Percent_Identity=25.2, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI166795287, Length=215, Percent_Identity=35.3488372093023, Blast_Score=84, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=423, Percent_Identity=39.4799054373522, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI17537465, Length=254, Percent_Identity=31.1023622047244, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17538252, Length=199, Percent_Identity=33.6683417085427, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=192, Percent_Identity=31.7708333333333, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=161, Percent_Identity=35.4037267080745, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=191, Percent_Identity=30.8900523560209, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=188, Percent_Identity=30.8510638297872, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17538254, Length=196, Percent_Identity=28.0612244897959, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=438, Percent_Identity=35.1598173515982, Blast_Score=246, Evalue=6e-66,
Organism=Saccharomyces cerevisiae, GI6319685, Length=499, Percent_Identity=27.0541082164329, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=39.5918367346939, Blast_Score=311, Evalue=6e-85,
Organism=Drosophila melanogaster, GI24652985, Length=501, Percent_Identity=25.5489021956088, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI19922090, Length=501, Percent_Identity=25.5489021956088, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24652981, Length=501, Percent_Identity=25.5489021956088, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24652983, Length=501, Percent_Identity=25.5489021956088, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI45550774, Length=489, Percent_Identity=26.1758691206544, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648435, Length=489, Percent_Identity=26.1758691206544, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648437, Length=489, Percent_Identity=26.1758691206544, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24644968, Length=499, Percent_Identity=24.248496993988, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI161078093, Length=524, Percent_Identity=25.381679389313, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356731, Length=504, Percent_Identity=24.2063492063492, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648441, Length=426, Percent_Identity=25.5868544600939, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648439, Length=426, Percent_Identity=25.5868544600939, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53677; Mature: 53546

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFSADSVTSLRQLADALAARSVSAEELARTYLARIEQAGALNAFTHVDAERTLAQAREA
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHH
DARRARGEATLLTGVPVAHKDVFVTRGWRATAGSKMLANYESPFDATVVERMAAAGMVTL
HHHHHCCCEEEEECCCCCCCCEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCEEEE
GKTNMDEFAMGSSNENSFFGAVRNPWDTDRVPGGSSGGSAAAVAAGLAPAATGTDTGGSI
CCCCHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCC
RQPSSFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMAHTAEDCALLLNAMAGFDAKDST
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
SIPPEQGGVDEDFARYLGQPRAGASESQPLAGLRIGLPREYFGKGLSPDVEETVRAALRQ
CCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEECCCHHHHCCCCCCCHHHHHHHHHHH
YEQLGATLVEVSLPKTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAAEYRDLLDMYKKS
HHHHCCEEEEEECCCCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
RAEGFGAEVKRRIMVGTYVLSHGYYDAYYLQAQKIRRIIADDFQRAFAQCDVIMGPVAPT
HCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VAWKLGEKTSDPVQMYLADIFTLSTSLAGLPGMSVPCGFGDGNMPVGLQLIGNYFDEARL
HHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LQTAHAFQQATDWHLRRPAKA
HHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PFSADSVTSLRQLADALAARSVSAEELARTYLARIEQAGALNAFTHVDAERTLAQAREA
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHH
DARRARGEATLLTGVPVAHKDVFVTRGWRATAGSKMLANYESPFDATVVERMAAAGMVTL
HHHHHCCCEEEEECCCCCCCCEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCEEEE
GKTNMDEFAMGSSNENSFFGAVRNPWDTDRVPGGSSGGSAAAVAAGLAPAATGTDTGGSI
CCCCHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCC
RQPSSFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMAHTAEDCALLLNAMAGFDAKDST
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
SIPPEQGGVDEDFARYLGQPRAGASESQPLAGLRIGLPREYFGKGLSPDVEETVRAALRQ
CCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEECCCHHHHCCCCCCCHHHHHHHHHHH
YEQLGATLVEVSLPKTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAAEYRDLLDMYKKS
HHHHCCEEEEEECCCCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
RAEGFGAEVKRRIMVGTYVLSHGYYDAYYLQAQKIRRIIADDFQRAFAQCDVIMGPVAPT
HCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VAWKLGEKTSDPVQMYLADIFTLSTSLAGLPGMSVPCGFGDGNMPVGLQLIGNYFDEARL
HHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LQTAHAFQQATDWHLRRPAKA
HHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA