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The map label for this gene is dmpN [H]

Identifier: 73539505

GI number: 73539505

Start: 2514572

End: 2516116

Strand: Direct

Name: dmpN [H]

Synonym: Reut_B5683

Alternate gene names: 73539505

Gene position: 2514572-2516116 (Clockwise)

Preceding gene: 73539504

Following gene: 73539506

Centisome position: 92.24

GC content: 60.84

Gene sequence:

>1545_bases
ATGGATACCCGCGTTGCCAAGAAGAAGCTTGGCCTGAAGGAACGGTACGCCGCCATGACCCGCGGCCTGGGCTGGGAGAC
CACGTATCAGCCGATGGACAAGGTCTTTCCCTACGACACCTACGAAGGCATCAAGATCCACGACTGGGACAAGTGGCAGG
ACCCGTTCCGCCTGACGATGGACGCCTACTGGAAGTACCAGGGGGAGAAGGAAAAGAAGCTGTATGCGGTGATCGAAGCC
TTCGCGCAGAACAACGGCCAGCTTGGCGTCAGCGACGCCCGGTACATCAATGCACTGAAGCTGTTCCTTCAGGGCGTGAC
GCCGCTTGAATACTGCGCTCACCGTGGCTTTGCACATGTTGGCCGTCAGTTCACCGGCGAGGGCGCGCGCGTGGCCGCGC
AGATGCAGTCCATCGACGAGCTGCGTCACTACCAGACCGAGACGCACGCGATCTCGCACTACAACAAGTACTTCAACGGG
CTGCACCGGGCGCACCATTGGTTCGATCGTGTCTGGTACCTGTCGGTGCCGAAGTCGTTCTTCGAGGATGCGTGCACCGC
CGGTCCGTTCGAGTTCCTGACGGCCGTGAGCTTTTCGTTCGAGTACGTGCTGACCAACCTGCTGTTCGTGCCCTTCATGT
CCGGCGCGGCGCACAACGGCGACATGAGCACCGTCACCTTCGGTTTCTCGGCGCAGTCCGACGAGTCGCGGCACATGACG
CTGGGTATCGAGTGCATCAAGTTCATGCTCGAGCAGGATTCCGGCAACGTGCCCATCGTGCAGCGCTGGATCGACAAGTG
GTTCTGGCGCGGTTACCGCCTGCTGACCATCGTGGCGATGATGCAGGACTACATGCTGCCCAAGCGCGTGATGAGCTGGA
AGGAGGCATGGGAAATGTACGCCGAGGGCAGCGGTGGTGCGCTGTTCAAGGACCTCGCGCGCTACGGCATTCGCGAACCA
CGCGGCTGGAAGGACGCCTGCGAGGGCAAGGACCATATCAGCCACCAGGCGTGGGCCACCTTCTACAACTACGGGGCCGC
GGCGCCTTTCCATACGTGGCTGCCGCAGGAAGAGGAAATGCAGTGGCTTTCGCAGAAGTATCCCGAGAGCTTCGACAAGC
TGTATCGCCCGCGCTTCGAGTACTGGCGCGAGCGCGCGGACGAAGGCCGCCGCTTCTACAACAAGACGCTGCCGATGCTG
TGTACCACCTGCCAGGTCCCGATGTTCTTCACGGAGCCCGGTGATGCCACGAAGATCTGCTACCGCGAGACCAGCTACTT
TGGCAACAAGTATCACTTCTGCTCCGACCACTGCCAGCACATCTTCGAGGATGAGCCCGAGAAGTACGTGCAGAGCTGGC
TGCCGGTGCACCAAATCTACCAGGGGCACTGCTTCCCCGAAGGCACGGACCCGACGGCGGAAGGCTTCGATCCGCTGACA
GCCGTCCTGCAGTACTACGAAATGAACATCGGACGGGACAACTTCGACTTCGAGGGATCGGAGGACCAGAAGAACTTTGC
CGCCTGGACAGGAGAGAAAGCATGA

Upstream 100 bases:

>100_bases
GGCCACATCGACGAAGACGACGACCAGCTCACGTTGAGCTGGAACCACTGAACGCTGCTGGACGCACAGCACCGCACATA
CCGACAAGAGGAGACACACC

Downstream 100 bases:

>100_bases
GCGTCGTCGCCAATCCCGCTGCCGGGGTGCCTTACCAGTTCCCCATGAAGGACGTTCGTGAGAACTTCCCCGCGCCGCTG
CTGTACATCGGATGGGAAGA

Product: methane/phenol/toluene hydroxylase

Products: NA

Alternate protein names: Phenol 2-monooxygenase P3 component [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MDTRVAKKKLGLKERYAAMTRGLGWETTYQPMDKVFPYDTYEGIKIHDWDKWQDPFRLTMDAYWKYQGEKEKKLYAVIEA
FAQNNGQLGVSDARYINALKLFLQGVTPLEYCAHRGFAHVGRQFTGEGARVAAQMQSIDELRHYQTETHAISHYNKYFNG
LHRAHHWFDRVWYLSVPKSFFEDACTAGPFEFLTAVSFSFEYVLTNLLFVPFMSGAAHNGDMSTVTFGFSAQSDESRHMT
LGIECIKFMLEQDSGNVPIVQRWIDKWFWRGYRLLTIVAMMQDYMLPKRVMSWKEAWEMYAEGSGGALFKDLARYGIREP
RGWKDACEGKDHISHQAWATFYNYGAAAPFHTWLPQEEEMQWLSQKYPESFDKLYRPRFEYWRERADEGRRFYNKTLPML
CTTCQVPMFFTEPGDATKICYRETSYFGNKYHFCSDHCQHIFEDEPEKYVQSWLPVHQIYQGHCFPEGTDPTAEGFDPLT
AVLQYYEMNIGRDNFDFEGSEDQKNFAAWTGEKA

Sequences:

>Translated_514_residues
MDTRVAKKKLGLKERYAAMTRGLGWETTYQPMDKVFPYDTYEGIKIHDWDKWQDPFRLTMDAYWKYQGEKEKKLYAVIEA
FAQNNGQLGVSDARYINALKLFLQGVTPLEYCAHRGFAHVGRQFTGEGARVAAQMQSIDELRHYQTETHAISHYNKYFNG
LHRAHHWFDRVWYLSVPKSFFEDACTAGPFEFLTAVSFSFEYVLTNLLFVPFMSGAAHNGDMSTVTFGFSAQSDESRHMT
LGIECIKFMLEQDSGNVPIVQRWIDKWFWRGYRLLTIVAMMQDYMLPKRVMSWKEAWEMYAEGSGGALFKDLARYGIREP
RGWKDACEGKDHISHQAWATFYNYGAAAPFHTWLPQEEEMQWLSQKYPESFDKLYRPRFEYWRERADEGRRFYNKTLPML
CTTCQVPMFFTEPGDATKICYRETSYFGNKYHFCSDHCQHIFEDEPEKYVQSWLPVHQIYQGHCFPEGTDPTAEGFDPLT
AVLQYYEMNIGRDNFDFEGSEDQKNFAAWTGEKA
>Mature_514_residues
MDTRVAKKKLGLKERYAAMTRGLGWETTYQPMDKVFPYDTYEGIKIHDWDKWQDPFRLTMDAYWKYQGEKEKKLYAVIEA
FAQNNGQLGVSDARYINALKLFLQGVTPLEYCAHRGFAHVGRQFTGEGARVAAQMQSIDELRHYQTETHAISHYNKYFNG
LHRAHHWFDRVWYLSVPKSFFEDACTAGPFEFLTAVSFSFEYVLTNLLFVPFMSGAAHNGDMSTVTFGFSAQSDESRHMT
LGIECIKFMLEQDSGNVPIVQRWIDKWFWRGYRLLTIVAMMQDYMLPKRVMSWKEAWEMYAEGSGGALFKDLARYGIREP
RGWKDACEGKDHISHQAWATFYNYGAAAPFHTWLPQEEEMQWLSQKYPESFDKLYRPRFEYWRERADEGRRFYNKTLPML
CTTCQVPMFFTEPGDATKICYRETSYFGNKYHFCSDHCQHIFEDEPEKYVQSWLPVHQIYQGHCFPEGTDPTAEGFDPLT
AVLQYYEMNIGRDNFDFEGSEDQKNFAAWTGEKA

Specific function: Catabolizes phenol, and some of its methylated derivatives. P3 is required for growth on phenol, and for in vitro phenol hydroxylase activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR003430
- InterPro:   IPR012348
- InterPro:   IPR007029 [H]

Pfam domain/function: PF02332 Phenol_Hydrox; PF04945 YHS [H]

EC number: =1.14.13.7 [H]

Molecular weight: Translated: 60341; Mature: 60341

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure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HHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2254258 [H]