Click here to switch to the map view.

The map label for this gene is flgJ [H]

Identifier: 73539455

GI number: 73539455

Start: 2451436

End: 2452482

Strand: Direct

Name: flgJ [H]

Synonym: Reut_B5633

Alternate gene names: 73539455

Gene position: 2451436-2452482 (Clockwise)

Preceding gene: 73539454

Following gene: 73539456

Centisome position: 89.92

GC content: 67.62

Gene sequence:

>1047_bases
ATGGACAGCGCTCTCTCCGCGCAGGCGGATGTATCGCAGCGCTTTGCCCTCGACACGCAAGGCTTCGAGGCGCTCAAGCA
AAGCGCGCGCAGCGGCGCTGACACAGGCACGCTGCGTGCCGCCGCGAAGCAGTTTGAGGCCGTCTTCACGCAGATGGTGC
TCAAGAGCATGCGCGATGCCACGCCGCAGGACGGTGTGTTCGACAGCGAGCAAGGCAAGCTCTACATGTCGATGCTGGAC
CAGCAGCTTGCACAGCAGATGTCTTCGCGCGGCATCGGCCTGGCCGAAGTGATGGTGCGTCAGCTTGCGCGCGCCACGGG
TGCGCAGGTTCCACCGGGCATGATCAGTGCCAACGCCGCGCCGGGTGCCACCGGCACCGCGGCAGCCGATGCCGAGATGG
CACGGCTGCTGGACGGCCGCGGCGCCGGCGCGGCGACTGCTCAGGATGGCGACGCACCGGCCATCGGCACCGTGGTGCCG
GGCGCGAGCTGGAACCCGACGGCGGGCCTGCGGCAATACCGCGAGCAGGACTATGCCTGGCAGGGACAAGGGCAGGGCGG
TAGCAATCTCGGGCCGTTGCCCGACGATGCACCGGCGCACGTGAGCGCGTTTGTATCGCGCATGTCCGCACCCGCCGAAG
CAGCTTCGCGCGTCAGCGGCGTGCCGGCGCGGCTGATCGTCGGTCAGGCGGCATTGGAATCGGGCTGGGGCCGCCGCGAA
ATTACGCATCCCGACGGCAGCACCACGTTCAATGTGTTCGGCATCAAGGCCGGCGCGAACTGGAAGGGCCGCGTGGCTGA
AGTCACCACGACCGAGTATGTCGATGGCCAGCCGCAGAAGGTGCGCGCGCGCTTCCGCGCCTATGGCTCGTATGACGAGG
CGTGTGCCGACTACGCACGGCTGCTGACCACCAATCCGCGTTACGCGGCCGTGGTGTCGGCAGGCTCTGCCGACGAAGCA
GCGCATGGCCTGCAACGCGCGGGTTATGCCACCGATCCTGCCTACGGGCAGAAGCTGGTGCAGATCATGAAGAAGGTGTC
GGCGTAA

Upstream 100 bases:

>100_bases
AGGGTCTGAATGCACTGGGCGCAACGCCGGCGGACCTGCAGACCATCCTGGAAGCGATGCGCGCGGCCGGGGCGCTGCGC
GCCGAGCTGGAGATTATCTG

Downstream 100 bases:

>100_bases
GCGCTCGTCGACGAGTCCCTCCCTCTCTCGCTCTCGGGAGAGGGGCCGGAGTTGAAGGTTGGCGCACGCAGTTGCGACAC
CGCTCGTCCAGCGCGGATGA

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MDSALSAQADVSQRFALDTQGFEALKQSARSGADTGTLRAAAKQFEAVFTQMVLKSMRDATPQDGVFDSEQGKLYMSMLD
QQLAQQMSSRGIGLAEVMVRQLARATGAQVPPGMISANAAPGATGTAAADAEMARLLDGRGAGAATAQDGDAPAIGTVVP
GASWNPTAGLRQYREQDYAWQGQGQGGSNLGPLPDDAPAHVSAFVSRMSAPAEAASRVSGVPARLIVGQAALESGWGRRE
ITHPDGSTTFNVFGIKAGANWKGRVAEVTTTEYVDGQPQKVRARFRAYGSYDEACADYARLLTTNPRYAAVVSAGSADEA
AHGLQRAGYATDPAYGQKLVQIMKKVSA

Sequences:

>Translated_348_residues
MDSALSAQADVSQRFALDTQGFEALKQSARSGADTGTLRAAAKQFEAVFTQMVLKSMRDATPQDGVFDSEQGKLYMSMLD
QQLAQQMSSRGIGLAEVMVRQLARATGAQVPPGMISANAAPGATGTAAADAEMARLLDGRGAGAATAQDGDAPAIGTVVP
GASWNPTAGLRQYREQDYAWQGQGQGGSNLGPLPDDAPAHVSAFVSRMSAPAEAASRVSGVPARLIVGQAALESGWGRRE
ITHPDGSTTFNVFGIKAGANWKGRVAEVTTTEYVDGQPQKVRARFRAYGSYDEACADYARLLTTNPRYAAVVSAGSADEA
AHGLQRAGYATDPAYGQKLVQIMKKVSA
>Mature_348_residues
MDSALSAQADVSQRFALDTQGFEALKQSARSGADTGTLRAAAKQFEAVFTQMVLKSMRDATPQDGVFDSEQGKLYMSMLD
QQLAQQMSSRGIGLAEVMVRQLARATGAQVPPGMISANAAPGATGTAAADAEMARLLDGRGAGAATAQDGDAPAIGTVVP
GASWNPTAGLRQYREQDYAWQGQGQGGSNLGPLPDDAPAHVSAFVSRMSAPAEAASRVSGVPARLIVGQAALESGWGRRE
ITHPDGSTTFNVFGIKAGANWKGRVAEVTTTEYVDGQPQKVRARFRAYGSYDEACADYARLLTTNPRYAAVVSAGSADEA
AHGLQRAGYATDPAYGQKLVQIMKKVSA

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=352, Percent_Identity=40.9090909090909, Blast_Score=231, Evalue=6e-62,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 36366; Mature: 36366

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSALSAQADVSQRFALDTQGFEALKQSARSGADTGTLRAAAKQFEAVFTQMVLKSMRDA
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
TPQDGVFDSEQGKLYMSMLDQQLAQQMSSRGIGLAEVMVRQLARATGAQVPPGMISANAA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEECCCC
PGATGTAAADAEMARLLDGRGAGAATAQDGDAPAIGTVVPGASWNPTAGLRQYREQDYAW
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCC
QGQGQGGSNLGPLPDDAPAHVSAFVSRMSAPAEAASRVSGVPARLIVGQAALESGWGRRE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
ITHPDGSTTFNVFGIKAGANWKGRVAEVTTTEYVDGQPQKVRARFRAYGSYDEACADYAR
CCCCCCCCEEEEEEEEECCCCCCCEEEEEEHHHCCCCHHHHHHHHHHCCCHHHHHHHHHH
LLTTNPRYAAVVSAGSADEAAHGLQRAGYATDPAYGQKLVQIMKKVSA
HHCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDSALSAQADVSQRFALDTQGFEALKQSARSGADTGTLRAAAKQFEAVFTQMVLKSMRDA
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
TPQDGVFDSEQGKLYMSMLDQQLAQQMSSRGIGLAEVMVRQLARATGAQVPPGMISANAA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEECCCC
PGATGTAAADAEMARLLDGRGAGAATAQDGDAPAIGTVVPGASWNPTAGLRQYREQDYAW
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCC
QGQGQGGSNLGPLPDDAPAHVSAFVSRMSAPAEAASRVSGVPARLIVGQAALESGWGRRE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
ITHPDGSTTFNVFGIKAGANWKGRVAEVTTTEYVDGQPQKVRARFRAYGSYDEACADYAR
CCCCCCCCEEEEEEEEECCCCCCCEEEEEEHHHCCCCHHHHHHHHHHCCCHHHHHHHHHH
LLTTNPRYAAVVSAGSADEAAHGLQRAGYATDPAYGQKLVQIMKKVSA
HHCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]