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The map label for this gene is potD [C]

Identifier: 73539347

GI number: 73539347

Start: 2325722

End: 2326744

Strand: Direct

Name: potD [C]

Synonym: Reut_B5525

Alternate gene names: 73539347

Gene position: 2325722-2326744 (Clockwise)

Preceding gene: 73539346

Following gene: 73539350

Centisome position: 85.31

GC content: 63.15

Gene sequence:

>1023_bases
ATGTCCCGCATCACCCGCACCGCCACCGCACTGGCAGCGTTCGCGCTGCTGGCCGCCGCCCAGGCTCACGCCAACACCGT
GCTGACGGTCTACACGGCGCTCGAAGCCGACCAGATCGCCGCCTACAAGGCCGCGTTCGAGAAGGTGAACCCCGATATCG
AGATCAAGTGGGTACGTGACTCCACTGGCATCGTCACCGCCAAGCTGCTGGCCGAAAAGGCCGCGCCGCGCGCCGACGCC
GTGTGGGGTCTCGCTGGCTCGAGCCTCGCCATCCTCGACAAGGAAGGCATGCTGCAGCCGTACGCGCCGAAGAACCTCGC
CGCGATCGACGCGCAGTACCGCAGCGCGGCCAATCCGCCGGCATGGGTCGGCATGGACGTGTGGGGTGCGGCGATCTGCT
TCAACACCGTCGAAGCGCAGAAGCAGGGCCTGCCCAAGCCGACCTCGTGGGCGGACCTGACCAAGCCGGTGTACGCGGGC
AAGATCGTGATGCCGCATCCGGCGTCGTCGGGCACCGGCTTCCTGGATGTCAGCGCGTGGCTGCAGATGATGGGCGACAA
GAAGGGCTGGGACTACATGGACGCCTTGCACAAGAACATCGGCCTGTACACGCATTCCGGCTCCAAGCCGTGCAAGATGG
CCGCTCAGGGTGAGTTCCCGATCGGCATCGCGTTTGAATATCGTGCGATGAAGTCGAAGAAGGAAGGCGCGCCGATCGAT
ATCGTGCTGCCGAGCGAAGGCCTGGGCTGGGATATCGAGGCCACGGCGATTATCAAGGGCACGAAGAATCTCGAGGCTGC
GCGCAAGCTGGCTGACTTCTCGGCCTCTCGTGAGGCCATGGGCTTGTATGAGAAGAACTTCGCGGTGCTGGCGGTACCTG
GGGTTGCCAAGCCGGATGCCTTGCTGCCGGCGGATTATGAGAAGCGCTTGATCAAGAATGATTTTGTGTGGGCGAGTGCG
AATCGTGACCGCATCCTGACGGAATGGAGCAAGCGGTATGAAGGGAAGGCGGAAAAAAAGTAA

Upstream 100 bases:

>100_bases
TGCTGCACGCCGCCTCCGCCGGCTGGCTGCGCCGCACACAGGCCTGGCGCCGGCCGGCTTGAACCAATCGCGTACCCAAG
GCTTACCCAAAGGAGAAACC

Downstream 100 bases:

>100_bases
GGGGTGTTGAGGATGGGGCTGGCCGGGGGTGATCGCTCCTGGCCAGCGAAACAAGGCGGCCAGGAGCGATCACCCCCGCA
ATTCGGCAAGACCCAAAAAA

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MSRITRTATALAAFALLAAAQAHANTVLTVYTALEADQIAAYKAAFEKVNPDIEIKWVRDSTGIVTAKLLAEKAAPRADA
VWGLAGSSLAILDKEGMLQPYAPKNLAAIDAQYRSAANPPAWVGMDVWGAAICFNTVEAQKQGLPKPTSWADLTKPVYAG
KIVMPHPASSGTGFLDVSAWLQMMGDKKGWDYMDALHKNIGLYTHSGSKPCKMAAQGEFPIGIAFEYRAMKSKKEGAPID
IVLPSEGLGWDIEATAIIKGTKNLEAARKLADFSASREAMGLYEKNFAVLAVPGVAKPDALLPADYEKRLIKNDFVWASA
NRDRILTEWSKRYEGKAEKK

Sequences:

>Translated_340_residues
MSRITRTATALAAFALLAAAQAHANTVLTVYTALEADQIAAYKAAFEKVNPDIEIKWVRDSTGIVTAKLLAEKAAPRADA
VWGLAGSSLAILDKEGMLQPYAPKNLAAIDAQYRSAANPPAWVGMDVWGAAICFNTVEAQKQGLPKPTSWADLTKPVYAG
KIVMPHPASSGTGFLDVSAWLQMMGDKKGWDYMDALHKNIGLYTHSGSKPCKMAAQGEFPIGIAFEYRAMKSKKEGAPID
IVLPSEGLGWDIEATAIIKGTKNLEAARKLADFSASREAMGLYEKNFAVLAVPGVAKPDALLPADYEKRLIKNDFVWASA
NRDRILTEWSKRYEGKAEKK
>Mature_339_residues
SRITRTATALAAFALLAAAQAHANTVLTVYTALEADQIAAYKAAFEKVNPDIEIKWVRDSTGIVTAKLLAEKAAPRADAV
WGLAGSSLAILDKEGMLQPYAPKNLAAIDAQYRSAANPPAWVGMDVWGAAICFNTVEAQKQGLPKPTSWADLTKPVYAGK
IVMPHPASSGTGFLDVSAWLQMMGDKKGWDYMDALHKNIGLYTHSGSKPCKMAAQGEFPIGIAFEYRAMKSKKEGAPIDI
VLPSEGLGWDIEATAIIKGTKNLEAARKLADFSASREAMGLYEKNFAVLAVPGVAKPDALLPADYEKRLIKNDFVWASAN
RDRILTEWSKRYEGKAEKK

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG1840

COG function: function code P; ABC-type Fe3+ transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36819; Mature: 36687

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRITRTATALAAFALLAAAQAHANTVLTVYTALEADQIAAYKAAFEKVNPDIEIKWVRD
CCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHCHHHHHHHHHHHHHCCCCEEEEEEEC
STGIVTAKLLAEKAAPRADAVWGLAGSSLAILDKEGMLQPYAPKNLAAIDAQYRSAANPP
CCCEEHHHHHHHHCCCCCHHEEECCCCCEEEEECCCCCCCCCCCCCEEHHHHHHCCCCCC
AWVGMDVWGAAICFNTVEAQKQGLPKPTSWADLTKPVYAGKIVMPHPASSGTGFLDVSAW
CEECHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHCCEEEECCCCCCCCCEEEHHHH
LQMMGDKKGWDYMDALHKNIGLYTHSGSKPCKMAAQGEFPIGIAFEYRAMKSKKEGAPID
HHHHCCCCCCHHHHHHHHHCCEEECCCCCCCEEEECCCCCEEEEEEHHHHHCCCCCCCEE
IVLPSEGLGWDIEATAIIKGTKNLEAARKLADFSASREAMGLYEKNFAVLAVPGVAKPDA
EEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHCCEEEEEECCCCCCCC
LLPADYEKRLIKNDFVWASANRDRILTEWSKRYEGKAEKK
CCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SRITRTATALAAFALLAAAQAHANTVLTVYTALEADQIAAYKAAFEKVNPDIEIKWVRD
CCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHCHHHHHHHHHHHHHCCCCEEEEEEEC
STGIVTAKLLAEKAAPRADAVWGLAGSSLAILDKEGMLQPYAPKNLAAIDAQYRSAANPP
CCCEEHHHHHHHHCCCCCHHEEECCCCCEEEEECCCCCCCCCCCCCEEHHHHHHCCCCCC
AWVGMDVWGAAICFNTVEAQKQGLPKPTSWADLTKPVYAGKIVMPHPASSGTGFLDVSAW
CEECHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHCCEEEECCCCCCCCCEEEHHHH
LQMMGDKKGWDYMDALHKNIGLYTHSGSKPCKMAAQGEFPIGIAFEYRAMKSKKEGAPID
HHHHCCCCCCHHHHHHHHHCCEEECCCCCCCEEEECCCCCEEEEEEHHHHHCCCCCCCEE
IVLPSEGLGWDIEATAIIKGTKNLEAARKLADFSASREAMGLYEKNFAVLAVPGVAKPDA
EEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHCCEEEEEECCCCCCCC
LLPADYEKRLIKNDFVWASANRDRILTEWSKRYEGKAEKK
CCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]