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The map label for this gene is dhbE [H]

Identifier: 73539306

GI number: 73539306

Start: 2280505

End: 2282202

Strand: Reverse

Name: dhbE [H]

Synonym: Reut_B5484

Alternate gene names: 73539306

Gene position: 2282202-2280505 (Counterclockwise)

Preceding gene: 73539307

Following gene: 73539305

Centisome position: 83.72

GC content: 65.19

Gene sequence:

>1698_bases
ATGACCGGCATTCATTTCCCGATTGAAGGCGTGGCGTACTGCCCGCCGGCAGACGCTGAACGGTACTTCAGAAGCGGTGC
ATGGATCGACGACACGCTCGGCGGCGCACTGCGCAAGACCGCACGGCGCGCGCCGGACCAGGCCGCGTTCATCAGCGACG
AGCGGACGATCACGTTTGCGGAACTCGATGCGCTCAGCGAGCGCCTCGGCGCCGCGCTGCACCAGCTCGGCCTGCTTCCG
GGCGACCGTGCCATCTTCCAGATGGGCACCACGATCGACTCGGTCATCGCGCTGATTGGCTGCTACAAGGCAGGCGTCGT
TCCCGTGTGCGCGGTGCCGCAATACCGTGAAGTCGAGATCGGCCAGCTTTCCGCGCAGTCGGAACCCCGCGCCTACTTCG
TGCAAGCCGATTTCAGTGCATTCGACCTGACCGGCTTTGCCCGCGGCATGGCGCAGCGCCACCCGTCGATCGAACACCTG
TTCGTCGCGCGGGGCGGCAGTCCTGCGGCACAGGGCCAGGTTGCCGAACATGGCATGGATGCGCTCATCGAGTCGATCCC
TTACGAACAGGCTGTCGAAGTGCTGGCCGGCGTTCCGATCGGCAGCGAGGACGTGCTCAGCTTCCAGCTTTCCGGCGGCA
CGACCGGCGTACCGAAGATCATTCCGCGCTTTCATGCCGAATATATCGGCCATGCGCTGGCCTGCATGCGCCAGCTCGGC
CAGACCGCGCAAAGCCGGCTGATCTGGTCGCTGCCGCTGCTGCACAACGCGGGACAGGTCTACGTGCTGGTGCCAGCGGT
GGCGGGTGGCATGACGTCGGTGCTGATGCCGCGCGTCGATATCGCGCGGATGCTGGAACTGATCGAACAGCATCGCGTCA
CGCATGGCATGTCGATTGGCCCGGTCGCGCCGCAGATGATCGCGTACCAGCACATCGCGCGGCACGACCTGTCGTCGCTG
GAGCTGTTCGGCACGATGACGCGCGCCGATGCGCTCGAAGCGCATCTCGGCGTGCCCTGCTTCAACCTGTTCGGCACCAC
CGAAGGATTGCTGCTCGGTGGCGGCGCGCGCTTCCCCTCCGCGATGCGACATCGCACCCAGGGCTTCTCTGGTTGCCCCG
ATGACGATCTCCGCCTGCTGGTGCCCGGCACGGACGAAGCTGCTGCACCGGGAACGCCGGGCGAACTCTGCTTCCGTGGC
CCGTCGAGCCTGCGCGGCTACTACAAGGCGCCGGAGGCCACCGCGCAGTCCCTGACCGCGGATGGGTTCGTTCGCTCCGG
CGACCTCATGACCGAGCACGTGATCGACGGCTACCGTTGCTTCGCATTTGAAGGCCGCCTGCGCGACAACGTGAACCGGG
GCGGCGAAAAGATCGGCTCTGAAGAAGTGGAGGCGTATGTCAGCCGGCATCCGGCAGTCGCGGATGCCAAGCTCGTCGCG
ATGCCAGATCCGCTGTACGGCGAGAAGGGTTGCATCTACGTGATCCTGCGCGCGGGCCACGTAGCGCCCGATGTGACCGA
ACTCGCAGCATTCCTCGTAGAACAGGGACTCGCGAAATTCAAATGTCCCGAGCGAATTGAAGTTGTCGCCGAGTTTCCGG
TGACGCGGGTCGGCAAGGTCGACAAGGCCGCCTTGCGCGCCATGGTCGCGCGCCAGCTTGAAAACGAAGCCACGGAATTG
GGAAGGAAGTCAGCATGA

Upstream 100 bases:

>100_bases
CTTTACCGGCGGCCTCGACGGCAGGATAGAGCGATTCGACCGCGATATCGTGCGCATCGTGCCGCGCGATGCCGCGGCGC
AATGAGTTCCGGAGGCCATC

Downstream 100 bases:

>100_bases
AGAGCCGTACTTACCGAATCGGGCAGATCGTGCCCAGCTCCAACACCACGATGGAGACCGAGATTCCCGCCATGCTGCGC
GCACGGGAACAGATCCTGCC

Product: AMP-dependent synthetase and ligase

Products: (2,3-Dihydroxybenzoyl)adenylate; Diphosphate [C]

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 565; Mature: 564

Protein sequence:

>565_residues
MTGIHFPIEGVAYCPPADAERYFRSGAWIDDTLGGALRKTARRAPDQAAFISDERTITFAELDALSERLGAALHQLGLLP
GDRAIFQMGTTIDSVIALIGCYKAGVVPVCAVPQYREVEIGQLSAQSEPRAYFVQADFSAFDLTGFARGMAQRHPSIEHL
FVARGGSPAAQGQVAEHGMDALIESIPYEQAVEVLAGVPIGSEDVLSFQLSGGTTGVPKIIPRFHAEYIGHALACMRQLG
QTAQSRLIWSLPLLHNAGQVYVLVPAVAGGMTSVLMPRVDIARMLELIEQHRVTHGMSIGPVAPQMIAYQHIARHDLSSL
ELFGTMTRADALEAHLGVPCFNLFGTTEGLLLGGGARFPSAMRHRTQGFSGCPDDDLRLLVPGTDEAAAPGTPGELCFRG
PSSLRGYYKAPEATAQSLTADGFVRSGDLMTEHVIDGYRCFAFEGRLRDNVNRGGEKIGSEEVEAYVSRHPAVADAKLVA
MPDPLYGEKGCIYVILRAGHVAPDVTELAAFLVEQGLAKFKCPERIEVVAEFPVTRVGKVDKAALRAMVARQLENEATEL
GRKSA

Sequences:

>Translated_565_residues
MTGIHFPIEGVAYCPPADAERYFRSGAWIDDTLGGALRKTARRAPDQAAFISDERTITFAELDALSERLGAALHQLGLLP
GDRAIFQMGTTIDSVIALIGCYKAGVVPVCAVPQYREVEIGQLSAQSEPRAYFVQADFSAFDLTGFARGMAQRHPSIEHL
FVARGGSPAAQGQVAEHGMDALIESIPYEQAVEVLAGVPIGSEDVLSFQLSGGTTGVPKIIPRFHAEYIGHALACMRQLG
QTAQSRLIWSLPLLHNAGQVYVLVPAVAGGMTSVLMPRVDIARMLELIEQHRVTHGMSIGPVAPQMIAYQHIARHDLSSL
ELFGTMTRADALEAHLGVPCFNLFGTTEGLLLGGGARFPSAMRHRTQGFSGCPDDDLRLLVPGTDEAAAPGTPGELCFRG
PSSLRGYYKAPEATAQSLTADGFVRSGDLMTEHVIDGYRCFAFEGRLRDNVNRGGEKIGSEEVEAYVSRHPAVADAKLVA
MPDPLYGEKGCIYVILRAGHVAPDVTELAAFLVEQGLAKFKCPERIEVVAEFPVTRVGKVDKAALRAMVARQLENEATEL
GRKSA
>Mature_564_residues
TGIHFPIEGVAYCPPADAERYFRSGAWIDDTLGGALRKTARRAPDQAAFISDERTITFAELDALSERLGAALHQLGLLPG
DRAIFQMGTTIDSVIALIGCYKAGVVPVCAVPQYREVEIGQLSAQSEPRAYFVQADFSAFDLTGFARGMAQRHPSIEHLF
VARGGSPAAQGQVAEHGMDALIESIPYEQAVEVLAGVPIGSEDVLSFQLSGGTTGVPKIIPRFHAEYIGHALACMRQLGQ
TAQSRLIWSLPLLHNAGQVYVLVPAVAGGMTSVLMPRVDIARMLELIEQHRVTHGMSIGPVAPQMIAYQHIARHDLSSLE
LFGTMTRADALEAHLGVPCFNLFGTTEGLLLGGGARFPSAMRHRTQGFSGCPDDDLRLLVPGTDEAAAPGTPGELCFRGP
SSLRGYYKAPEATAQSLTADGFVRSGDLMTEHVIDGYRCFAFEGRLRDNVNRGGEKIGSEEVEAYVSRHPAVADAKLVAM
PDPLYGEKGCIYVILRAGHVAPDVTELAAFLVEQGLAKFKCPERIEVVAEFPVTRVGKVDKAALRAMVARQLENEATELG
RKSA

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=590, Percent_Identity=23.728813559322, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI38505220, Length=541, Percent_Identity=23.6598890942699, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI187761345, Length=552, Percent_Identity=25.9057971014493, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI187761343, Length=552, Percent_Identity=25.9057971014493, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI115511026, Length=554, Percent_Identity=23.826714801444, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI58082049, Length=551, Percent_Identity=24.5009074410163, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI157311624, Length=545, Percent_Identity=24.2201834862385, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI157311622, Length=545, Percent_Identity=24.2201834862385, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI28416953, Length=577, Percent_Identity=25.1299826689775, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI42544132, Length=539, Percent_Identity=23.0055658627087, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI122937307, Length=539, Percent_Identity=23.5621521335807, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1786810, Length=555, Percent_Identity=33.1531531531532, Blast_Score=251, Evalue=6e-68,
Organism=Escherichia coli, GI145693145, Length=554, Percent_Identity=27.4368231046931, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1788107, Length=568, Percent_Identity=25.3521126760563, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI221142682, Length=518, Percent_Identity=22.7799227799228, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=559, Percent_Identity=25.4025044722719, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71994703, Length=553, Percent_Identity=23.869801084991, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71994694, Length=553, Percent_Identity=23.869801084991, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71994690, Length=553, Percent_Identity=23.869801084991, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17559526, Length=570, Percent_Identity=23.859649122807, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17538037, Length=551, Percent_Identity=23.956442831216, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI32563687, Length=518, Percent_Identity=20.8494208494208, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17558820, Length=508, Percent_Identity=21.4566929133858, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17531443, Length=536, Percent_Identity=21.455223880597, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17557194, Length=548, Percent_Identity=22.0802919708029, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=408, Percent_Identity=23.0392156862745, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=365, Percent_Identity=24.6575342465753, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323182, Length=618, Percent_Identity=23.9482200647249, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24581924, Length=562, Percent_Identity=23.4875444839858, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21355181, Length=497, Percent_Identity=20.9255533199195, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21358303, Length=485, Percent_Identity=22.8865979381443, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI18859661, Length=528, Percent_Identity=22.9166666666667, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 2.7.7.58; 2.3.1.- [C]

Molecular weight: Translated: 60762; Mature: 60631

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGIHFPIEGVAYCPPADAERYFRSGAWIDDTLGGALRKTARRAPDQAAFISDERTITFA
CCCCCCCCCCEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCEEEHH
ELDALSERLGAALHQLGLLPGDRAIFQMGTTIDSVIALIGCYKAGVVPVCAVPQYREVEI
HHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCEECCCCCCCCCC
GQLSAQSEPRAYFVQADFSAFDLTGFARGMAQRHPSIEHLFVARGGSPAAQGQVAEHGMD
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHCCHHHHHHHH
ALIESIPYEQAVEVLAGVPIGSEDVLSFQLSGGTTGVPKIIPRFHAEYIGHALACMRQLG
HHHHHCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QTAQSRLIWSLPLLHNAGQVYVLVPAVAGGMTSVLMPRVDIARMLELIEQHRVTHGMSIG
HHHHHHEEEEEHHHCCCCEEEEEECCHHCCHHHHHHCHHHHHHHHHHHHHHHHHCCCCCC
PVAPQMIAYQHIARHDLSSLELFGTMTRADALEAHLGVPCFNLFGTTEGLLLGGGARFPS
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEECCCCCCH
AMRHRTQGFSGCPDDDLRLLVPGTDEAAAPGTPGELCFRGPSSLRGYYKAPEATAQSLTA
HHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEECCHHHHCCCCCCCHHHHHHHHH
DGFVRSGDLMTEHVIDGYRCFAFEGRLRDNVNRGGEKIGSEEVEAYVSRHPAVADAKLVA
CCCEECCCHHHHHHHCCEEEEEECCHHHHHHHHCHHHCCHHHHHHHHHCCCCCCCCEEEE
MPDPLYGEKGCIYVILRAGHVAPDVTELAAFLVEQGLAKFKCPERIEVVAEFPVTRVGKV
CCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHCCCH
DKAALRAMVARQLENEATELGRKSA
HHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TGIHFPIEGVAYCPPADAERYFRSGAWIDDTLGGALRKTARRAPDQAAFISDERTITFA
CCCCCCCCCEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCEEEHH
ELDALSERLGAALHQLGLLPGDRAIFQMGTTIDSVIALIGCYKAGVVPVCAVPQYREVEI
HHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCEECCCCCCCCCC
GQLSAQSEPRAYFVQADFSAFDLTGFARGMAQRHPSIEHLFVARGGSPAAQGQVAEHGMD
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHCCHHHHHHHH
ALIESIPYEQAVEVLAGVPIGSEDVLSFQLSGGTTGVPKIIPRFHAEYIGHALACMRQLG
HHHHHCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QTAQSRLIWSLPLLHNAGQVYVLVPAVAGGMTSVLMPRVDIARMLELIEQHRVTHGMSIG
HHHHHHEEEEEHHHCCCCEEEEEECCHHCCHHHHHHCHHHHHHHHHHHHHHHHHCCCCCC
PVAPQMIAYQHIARHDLSSLELFGTMTRADALEAHLGVPCFNLFGTTEGLLLGGGARFPS
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEECCCCCCH
AMRHRTQGFSGCPDDDLRLLVPGTDEAAAPGTPGELCFRGPSSLRGYYKAPEATAQSLTA
HHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEECCHHHHCCCCCCCHHHHHHHHH
DGFVRSGDLMTEHVIDGYRCFAFEGRLRDNVNRGGEKIGSEEVEAYVSRHPAVADAKLVA
CCCEECCCHHHHHHHCCEEEEEECCHHHHHHHHCHHHCCHHHHHHHHHCCCCCCCCEEEE
MPDPLYGEKGCIYVILRAGHVAPDVTELAAFLVEQGLAKFKCPERIEVVAEFPVTRVGKV
CCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHCCCH
DKAALRAMVARQLENEATELGRKSA
HHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,3-Dihydroxybenzoate; ATP [C]

Specific reaction: 2,3-Dihydroxybenzoate + ATP <==> (2,3-Dihydroxybenzoyl)adenylate + Diphosphate [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]