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The map label for this gene is ynfL [H]

Identifier: 73539258

GI number: 73539258

Start: 2226290

End: 2227189

Strand: Reverse

Name: ynfL [H]

Synonym: Reut_B5436

Alternate gene names: 73539258

Gene position: 2227189-2226290 (Counterclockwise)

Preceding gene: 73539262

Following gene: 73539253

Centisome position: 81.7

GC content: 68.56

Gene sequence:

>900_bases
ATGGAATTCCGACACCTGCGCTATTTCCTGGTCCTGGCCGAGGAACTGCACTTCGGCCGCGCCGCGAAGCGGCTGTCGAT
CTCCCAGCCGCCGCTGTCGCTCAATATCCAGCAGCTGGAGGCTTCGGTCGGGGCAAAGCTGTTCGAGCGTGACAGCCGCG
GGGTGCGGCTGACAGCAGCCGGCCGGGCATTCCGCGAATCGGCCAACGCGCTGCTGGCGCAGGCCGAAGCGGCGCGCGTG
CTGGCGCGCGAGATCGAAGCCGGGGCGGTCGGGCGGCTGCGCGTGGGCTTTGTCGGGTCGATGTTGTACCGGGGGCTGCC
ACAGCACCTGCGCGACTTCCAGGCGGGCTATCCGGGCATCCACGTGGCGCTGACCGAACTCAACTCGCAGGAGCAGATCG
ACGCGCTGCTGCACAACGAACTGGACGCCGGGTTCGTCCATACGGGCCGCCTGCCCGATGAACTGAGCGCCGTACTGATC
CATAGCGAGCCCTTCGTCTGCTGCGTGCCGCAGGACCATGCGCTGGCAGCACAGGCTGAGGTCGCGCTGGTGGACCTGCG
CGCCGAGCCGTTCGTGCTGTTCTCGCGCAAGGCGTCACCCGATTACTACAGCCGGATCTTCGATATGTGCGCGGCGCAGG
GCTTCTATCCGCAGATCCGGCACGAGGTGCGGCACTGGCTGTCGGTGGTGTCGCTGGTGTCGCAGGGGATGGGGGTGGCG
GTGGTACCGGCGGCCCTTGCGCGCTCGGGCATGGCTGGAGCGGCGTTCCGGCCACTGGCCGATGCCACGGTGCCGTCCGA
GGTTTACTGCGCGTGGAAAGCCGCGCCCGACCATCCGGCGCGCGATCATTTCGTGGCGATGGTCCGGGCAGCCACGGCAG
CACAACCGGCCGACGCCTGA

Upstream 100 bases:

>100_bases
TGGCTTGCCGGCGCGCGGACCTGTTTGCACCATTATTGGATTCCCATGGATTCGCGTCTAATTCTAAAATTGGCTTGATC
CATATAATTTTTGTATCGCC

Downstream 100 bases:

>100_bases
ACGGATTCCGGACCCCAAAGAAAAAGCCACCGGCCCAAAGGCTGGTGGCTTATGAAGTACGCGTGGAACGGGGTGTTCAG
CGTGAGTGCAGCTTAACTCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MEFRHLRYFLVLAEELHFGRAAKRLSISQPPLSLNIQQLEASVGAKLFERDSRGVRLTAAGRAFRESANALLAQAEAARV
LAREIEAGAVGRLRVGFVGSMLYRGLPQHLRDFQAGYPGIHVALTELNSQEQIDALLHNELDAGFVHTGRLPDELSAVLI
HSEPFVCCVPQDHALAAQAEVALVDLRAEPFVLFSRKASPDYYSRIFDMCAAQGFYPQIRHEVRHWLSVVSLVSQGMGVA
VVPAALARSGMAGAAFRPLADATVPSEVYCAWKAAPDHPARDHFVAMVRAATAAQPADA

Sequences:

>Translated_299_residues
MEFRHLRYFLVLAEELHFGRAAKRLSISQPPLSLNIQQLEASVGAKLFERDSRGVRLTAAGRAFRESANALLAQAEAARV
LAREIEAGAVGRLRVGFVGSMLYRGLPQHLRDFQAGYPGIHVALTELNSQEQIDALLHNELDAGFVHTGRLPDELSAVLI
HSEPFVCCVPQDHALAAQAEVALVDLRAEPFVLFSRKASPDYYSRIFDMCAAQGFYPQIRHEVRHWLSVVSLVSQGMGVA
VVPAALARSGMAGAAFRPLADATVPSEVYCAWKAAPDHPARDHFVAMVRAATAAQPADA
>Mature_299_residues
MEFRHLRYFLVLAEELHFGRAAKRLSISQPPLSLNIQQLEASVGAKLFERDSRGVRLTAAGRAFRESANALLAQAEAARV
LAREIEAGAVGRLRVGFVGSMLYRGLPQHLRDFQAGYPGIHVALTELNSQEQIDALLHNELDAGFVHTGRLPDELSAVLI
HSEPFVCCVPQDHALAAQAEVALVDLRAEPFVLFSRKASPDYYSRIFDMCAAQGFYPQIRHEVRHWLSVVSLVSQGMGVA
VVPAALARSGMAGAAFRPLADATVPSEVYCAWKAAPDHPARDHFVAMVRAATAAQPADA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=279, Percent_Identity=38.7096774193548, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI2367136, Length=287, Percent_Identity=33.7979094076655, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1788887, Length=299, Percent_Identity=34.7826086956522, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI157672245, Length=264, Percent_Identity=32.5757575757576, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI145693105, Length=243, Percent_Identity=27.1604938271605, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1790399, Length=244, Percent_Identity=25, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790208, Length=300, Percent_Identity=27.6666666666667, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788748, Length=246, Percent_Identity=26.4227642276423, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787601, Length=259, Percent_Identity=24.7104247104247, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787806, Length=297, Percent_Identity=22.2222222222222, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788297, Length=274, Percent_Identity=27.007299270073, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87082132, Length=147, Percent_Identity=30.6122448979592, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081904, Length=210, Percent_Identity=29.5238095238095, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788481, Length=242, Percent_Identity=28.9256198347107, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789173, Length=148, Percent_Identity=33.1081081081081, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32570; Mature: 32570

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFRHLRYFLVLAEELHFGRAAKRLSISQPPLSLNIQQLEASVGAKLFERDSRGVRLTAA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEC
GRAFRESANALLAQAEAARVLAREIEAGAVGRLRVGFVGSMLYRGLPQHLRDFQAGYPGI
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCHHHHHHHHCCCCCE
HVALTELNSQEQIDALLHNELDAGFVHTGRLPDELSAVLIHSEPFVCCVPQDHALAAQAE
EEEEECCCCHHHHHHHHHCCCCCCEEECCCCCHHHHEEEEECCCEEEECCCCCCHHHHHE
VALVDLRAEPFVLFSRKASPDYYSRIFDMCAAQGFYPQIRHEVRHWLSVVSLVSQGMGVA
EEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
VVPAALARSGMAGAAFRPLADATVPSEVYCAWKAAPDHPARDHFVAMVRAATAAQPADA
EHHHHHHHCCCCCCHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEFRHLRYFLVLAEELHFGRAAKRLSISQPPLSLNIQQLEASVGAKLFERDSRGVRLTAA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEC
GRAFRESANALLAQAEAARVLAREIEAGAVGRLRVGFVGSMLYRGLPQHLRDFQAGYPGI
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCHHHHHHHHCCCCCE
HVALTELNSQEQIDALLHNELDAGFVHTGRLPDELSAVLIHSEPFVCCVPQDHALAAQAE
EEEEECCCCHHHHHHHHHCCCCCCEEECCCCCHHHHEEEEECCCEEEECCCCCCHHHHHE
VALVDLRAEPFVLFSRKASPDYYSRIFDMCAAQGFYPQIRHEVRHWLSVVSLVSQGMGVA
EEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
VVPAALARSGMAGAAFRPLADATVPSEVYCAWKAAPDHPARDHFVAMVRAATAAQPADA
EHHHHHHHCCCCCCHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]