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The map label for this gene is cspA [H]

Identifier: 73539164

GI number: 73539164

Start: 2112805

End: 2113008

Strand: Reverse

Name: cspA [H]

Synonym: Reut_B5342

Alternate gene names: 73539164

Gene position: 2113008-2112805 (Counterclockwise)

Preceding gene: 73539167

Following gene: 73539161

Centisome position: 77.51

GC content: 60.29

Gene sequence:

>204_bases
ATGGCAACCGGTACGGTCAAGTGGTTTAACGAGACTAAGGGCTTTGGCTTCATCACCCCGGACGACGGCGGTGCGGATCT
GTTCGCGCACTTCTCCGAAATCCAGGGTTCGGGCTTCAAGACGCTGAAGGACGGCCAACGCGTGACCTTCGAGGTCAAGC
AAGGTCCGAAGGGCCTGCAGGCATCGGCGATCAAGCCCGCCTGA

Upstream 100 bases:

>100_bases
GCCAACCTCGTCGGCTTCCGTTCGGTATCTACGAGAACTCAACGTGCTTGAACGGTTGTTTACGTGGTGTAAGAGAACTT
GCTTCGAAAAGGAAATTCCT

Downstream 100 bases:

>100_bases
TCTTGCGGCAGCCACGCAGTTGATGCGCGGCTGTCGTTTGAAAGCAAAAAAGGCAAGCTGCGTGCTTGCCTTTTTTGTTG
ACCCTGGCGGGTATCAGCCG

Product: cold-shock DNA-binding protein family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 67; Mature: 66

Protein sequence:

>67_residues
MATGTVKWFNETKGFGFITPDDGGADLFAHFSEIQGSGFKTLKDGQRVTFEVKQGPKGLQASAIKPA

Sequences:

>Translated_67_residues
MATGTVKWFNETKGFGFITPDDGGADLFAHFSEIQGSGFKTLKDGQRVTFEVKQGPKGLQASAIKPA
>Mature_66_residues
ATGTVKWFNETKGFGFITPDDGGADLFAHFSEIQGSGFKTLKDGQRVTFEVKQGPKGLQASAIKPA

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=62.6865671641791, Blast_Score=91, Evalue=1e-20,
Organism=Escherichia coli, GI1786841, Length=61, Percent_Identity=63.9344262295082, Blast_Score=87, Evalue=2e-19,
Organism=Escherichia coli, GI1788126, Length=61, Percent_Identity=63.9344262295082, Blast_Score=84, Evalue=2e-18,
Organism=Escherichia coli, GI1789979, Length=62, Percent_Identity=58.0645161290323, Blast_Score=80, Evalue=2e-17,
Organism=Escherichia coli, GI2367114, Length=62, Percent_Identity=54.8387096774194, Blast_Score=79, Evalue=6e-17,
Organism=Escherichia coli, GI1787834, Length=62, Percent_Identity=58.0645161290323, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=54.8387096774194, Blast_Score=76, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24658520, Length=61, Percent_Identity=47.5409836065574, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7163; Mature: 7032

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGTVKWFNETKGFGFITPDDGGADLFAHFSEIQGSGFKTLKDGQRVTFEVKQGPKGLQ
CCCCCEEECCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCC
ASAIKPA
CCCCCCC
>Mature Secondary Structure 
ATGTVKWFNETKGFGFITPDDGGADLFAHFSEIQGSGFKTLKDGQRVTFEVKQGPKGLQ
CCCCEEECCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCC
ASAIKPA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10483731 [H]