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The map label for this gene is cspA [H]

Identifier: 73539162

GI number: 73539162

Start: 2109822

End: 2110073

Strand: Direct

Name: cspA [H]

Synonym: Reut_B5340

Alternate gene names: 73539162

Gene position: 2109822-2110073 (Clockwise)

Preceding gene: 73539158

Following gene: 73539163

Centisome position: 77.39

GC content: 53.97

Gene sequence:

>252_bases
ATGGCGGTTTCGCCATTCGCTTTTTTTATTTTTTCTGGAAAGCAAATCATGCAAACCGGTATCGTCAAATGGTTCAACGA
CGCCAAGGGCTTCGGCTTCATCAAGCCGGACGCAGGTGGTGACGACCTCTTCGCCCACTTCTCCGAAATCCGCTCGGAAG
GCTTCAAGTCGCTGCAAGAGAACCAGCGCGTGTCGTTCGAAGTCAAGAACGGCCCGAAGGGTCTGCAAGCCGCGAACATC
ACCCCGCTGTAA

Upstream 100 bases:

>100_bases
AAATCGGCCTAGATTCAAGCGACGAGGCAACAGTTCATTCGTTAGCCACCGTCTGGTACTTCGGTTGTCCATGGTGTCCT
TTCCTTGAGTTCTATGTAGG

Downstream 100 bases:

>100_bases
GCGAGCGCGCGGCGCGCCTGGCGCGCTACGACAAGGAACCCGCCCACTGAGGCGGGTTTTTTTACGCCTGTTGGGGTCGC
GACTGCCGCTGTCGCGCCTT

Product: cold-shock DNA-binding protein family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 83; Mature: 82

Protein sequence:

>83_residues
MAVSPFAFFIFSGKQIMQTGIVKWFNDAKGFGFIKPDAGGDDLFAHFSEIRSEGFKSLQENQRVSFEVKNGPKGLQAANI
TPL

Sequences:

>Translated_83_residues
MAVSPFAFFIFSGKQIMQTGIVKWFNDAKGFGFIKPDAGGDDLFAHFSEIRSEGFKSLQENQRVSFEVKNGPKGLQAANI
TPL
>Mature_82_residues
AVSPFAFFIFSGKQIMQTGIVKWFNDAKGFGFIKPDAGGDDLFAHFSEIRSEGFKSLQENQRVSFEVKNGPKGLQAANIT
PL

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=53.7313432835821, Blast_Score=86, Evalue=5e-19,
Organism=Escherichia coli, GI1789979, Length=65, Percent_Identity=60, Blast_Score=85, Evalue=8e-19,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=59.375, Blast_Score=84, Evalue=2e-18,
Organism=Escherichia coli, GI1787834, Length=65, Percent_Identity=56.9230769230769, Blast_Score=80, Evalue=2e-17,
Organism=Escherichia coli, GI2367114, Length=65, Percent_Identity=53.8461538461538, Blast_Score=80, Evalue=4e-17,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=54.6875, Blast_Score=77, Evalue=3e-16,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=51.6129032258064, Blast_Score=73, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24658520, Length=66, Percent_Identity=42.4242424242424, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 9159; Mature: 9027

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSPFAFFIFSGKQIMQTGIVKWFNDAKGFGFIKPDAGGDDLFAHFSEIRSEGFKSLQE
CCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHC
NQRVSFEVKNGPKGLQAANITPL
CCCEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
AVSPFAFFIFSGKQIMQTGIVKWFNDAKGFGFIKPDAGGDDLFAHFSEIRSEGFKSLQE
CCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHC
NQRVSFEVKNGPKGLQAANITPL
CCCEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]