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The map label for this gene is citH [H]

Identifier: 73539127

GI number: 73539127

Start: 2067043

End: 2068380

Strand: Direct

Name: citH [H]

Synonym: Reut_B5304

Alternate gene names: 73539127

Gene position: 2067043-2068380 (Clockwise)

Preceding gene: 73539126

Following gene: 73539128

Centisome position: 75.82

GC content: 67.41

Gene sequence:

>1338_bases
ATGCCTCTCGACCCGACCACCATGGTGGCGCCCGCGCACCCGCCGGTATCCGGCCAGCAAGCGGACCACCCGGACCAGAA
GAAACCGATGTCGAAGGCGTCGGCCATCACGGCGATCACCATCGGCGGCGCGCTGGAACTGTATGACTCCGGCGTGTACA
ACTTCTTTGCCACGCTGATCATTCCGCTCTACTTTCCGGTGGGCAATCCGCTGGGCCAGCTGCTGCTGTCCTTCGGCACG
TTTGGCGCGGGCTACCTGATGCGCCCGCTCGGCGGCCTCGTGATCGGCGCCTACGCCGACCGCTATGGCCGCAAGCCGGC
GGTGCTGCTGTCGATGTGGCTGATGGGGCTCAGCGCGCTGATCCTTGTCGTGACGCCGCCCTATGCGCAGTTCGGCATCA
TGGCGCCGATCCTGATGATCGTCGCGCGCCTCGTCCAGGGCTTCGCGATCGGCGGAGAAATGGGCTCCGCCACCGCAATG
CTGATGGAGTATGCGGACGAACGTTCGCGCGGCTTCTACACCAGCTGGCAGACCGCCAGCCAGGGCATTGCCGCGGTCTT
CGCCGCGCTGGTTGCGCTCGCGCTCAGCCATACGCTGAGTCCGGACGCCCTGGAACACTGGGGCTGGCGCGTGGCCTTCT
TGATCGGCATCCTCGTCATTCCTGTCGGCCAGGTCATCCGCCGCCGGCTGGAAGAAACGCTAGAGGCCCCGGCTCGCAAG
AAGGCCGCGGCCGGCGGCTGGCAGCTGATGCGCGCGCACTGGCGCGAACTCGTGGCCAGCGTGCTGCTGATGACCGGCCT
GGCTGCCGCCGTGCACCTGATTGCGTACTACCTGCCCAACTACGCTTCGTTCCAGCTCAACATCCCGCGCGGCGACGCGG
TGTGGGCCGGCTTTGTCGCGGCAGGCATGATGGTGATCTTCGGGCCCATTGCAGGCTTGCTGAGCGACCGGGTCGGGCGC
CGCACCATGGTGTGGTGGTCGCGCATCGCGCTGCTGGTGATGGCCTATCCGGCCTTCGTGCTGATCAACACCTTCCCGTC
GCTGACTTGCCTGCTGGTAGTGGTCGGGTGCCTGGCCATCCCGATGGCGATGACGTCGCCCGCCACGCTGGTGCTGGTCA
GCGAGGTGCTGCCCCAGCGCCTGCGCGCGACCGGCATGGCGGTGACCTACTACGTGGCCATCGCGGTGTTCGGCGGCTTT
GCACAGCTGTTCTCGACCGTGCTGATCCACGTGACGGGCAGCCCCAATGCGCCGGCGTTCTACCTGATCGGCTGCGGGCT
GGTGTCGCTGATCGGGCTGCGGATGGTGCCGGAGACGCTGGGCCGGCGGCTCTCGTAG

Upstream 100 bases:

>100_bases
GCGCAGGCGCCGCCTGAGGCGGCGTCGCATTCAGATTCAGACGTCAACAACGCGGCAGCCACACATGGCTAACCGGTCTG
CCGCGCCCAGGAGACTTGCA

Downstream 100 bases:

>100_bases
TCGCATGCCGGCGCCCTTCACCGGGGCGCCGGTAAAATCGCGGCTTTGAACGCCGGGCAGCCTGCCCGGTACGCGCCGGA
GCCGTACGTGCCCTTTATCC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MPLDPTTMVAPAHPPVSGQQADHPDQKKPMSKASAITAITIGGALELYDSGVYNFFATLIIPLYFPVGNPLGQLLLSFGT
FGAGYLMRPLGGLVIGAYADRYGRKPAVLLSMWLMGLSALILVVTPPYAQFGIMAPILMIVARLVQGFAIGGEMGSATAM
LMEYADERSRGFYTSWQTASQGIAAVFAALVALALSHTLSPDALEHWGWRVAFLIGILVIPVGQVIRRRLEETLEAPARK
KAAAGGWQLMRAHWRELVASVLLMTGLAAAVHLIAYYLPNYASFQLNIPRGDAVWAGFVAAGMMVIFGPIAGLLSDRVGR
RTMVWWSRIALLVMAYPAFVLINTFPSLTCLLVVVGCLAIPMAMTSPATLVLVSEVLPQRLRATGMAVTYYVAIAVFGGF
AQLFSTVLIHVTGSPNAPAFYLIGCGLVSLIGLRMVPETLGRRLS

Sequences:

>Translated_445_residues
MPLDPTTMVAPAHPPVSGQQADHPDQKKPMSKASAITAITIGGALELYDSGVYNFFATLIIPLYFPVGNPLGQLLLSFGT
FGAGYLMRPLGGLVIGAYADRYGRKPAVLLSMWLMGLSALILVVTPPYAQFGIMAPILMIVARLVQGFAIGGEMGSATAM
LMEYADERSRGFYTSWQTASQGIAAVFAALVALALSHTLSPDALEHWGWRVAFLIGILVIPVGQVIRRRLEETLEAPARK
KAAAGGWQLMRAHWRELVASVLLMTGLAAAVHLIAYYLPNYASFQLNIPRGDAVWAGFVAAGMMVIFGPIAGLLSDRVGR
RTMVWWSRIALLVMAYPAFVLINTFPSLTCLLVVVGCLAIPMAMTSPATLVLVSEVLPQRLRATGMAVTYYVAIAVFGGF
AQLFSTVLIHVTGSPNAPAFYLIGCGLVSLIGLRMVPETLGRRLS
>Mature_444_residues
PLDPTTMVAPAHPPVSGQQADHPDQKKPMSKASAITAITIGGALELYDSGVYNFFATLIIPLYFPVGNPLGQLLLSFGTF
GAGYLMRPLGGLVIGAYADRYGRKPAVLLSMWLMGLSALILVVTPPYAQFGIMAPILMIVARLVQGFAIGGEMGSATAML
MEYADERSRGFYTSWQTASQGIAAVFAALVALALSHTLSPDALEHWGWRVAFLIGILVIPVGQVIRRRLEETLEAPARKK
AAAGGWQLMRAHWRELVASVLLMTGLAAAVHLIAYYLPNYASFQLNIPRGDAVWAGFVAAGMMVIFGPIAGLLSDRVGRR
TMVWWSRIALLVMAYPAFVLINTFPSLTCLLVVVGCLAIPMAMTSPATLVLVSEVLPQRLRATGMAVTYYVAIAVFGGFA
QLFSTVLIHVTGSPNAPAFYLIGCGLVSLIGLRMVPETLGRRLS

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=431, Percent_Identity=31.322505800464, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1788942, Length=385, Percent_Identity=33.7662337662338, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1788292, Length=427, Percent_Identity=26.6978922716628, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1789941, Length=440, Percent_Identity=28.6363636363636, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI87082231, Length=162, Percent_Identity=30.8641975308642, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47713; Mature: 47582

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLDPTTMVAPAHPPVSGQQADHPDQKKPMSKASAITAITIGGALELYDSGVYNFFATLI
CCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHEEEEECCHHHHHHCHHHHHHHHHH
IPLYFPVGNPLGQLLLSFGTFGAGYLMRPLGGLVIGAYADRYGRKPAVLLSMWLMGLSAL
HHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ILVVTPPYAQFGIMAPILMIVARLVQGFAIGGEMGSATAMLMEYADERSRGFYTSWQTAS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHH
QGIAAVFAALVALALSHTLSPDALEHWGWRVAFLIGILVIPVGQVIRRRLEETLEAPARK
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
KAAAGGWQLMRAHWRELVASVLLMTGLAAAVHLIAYYLPNYASFQLNIPRGDAVWAGFVA
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
AGMMVIFGPIAGLLSDRVGRRTMVWWSRIALLVMAYPAFVLINTFPSLTCLLVVVGCLAI
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PMAMTSPATLVLVSEVLPQRLRATGMAVTYYVAIAVFGGFAQLFSTVLIHVTGSPNAPAF
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEE
YLIGCGLVSLIGLRMVPETLGRRLS
HHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PLDPTTMVAPAHPPVSGQQADHPDQKKPMSKASAITAITIGGALELYDSGVYNFFATLI
CCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHEEEEECCHHHHHHCHHHHHHHHHH
IPLYFPVGNPLGQLLLSFGTFGAGYLMRPLGGLVIGAYADRYGRKPAVLLSMWLMGLSAL
HHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ILVVTPPYAQFGIMAPILMIVARLVQGFAIGGEMGSATAMLMEYADERSRGFYTSWQTAS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHH
QGIAAVFAALVALALSHTLSPDALEHWGWRVAFLIGILVIPVGQVIRRRLEETLEAPARK
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
KAAAGGWQLMRAHWRELVASVLLMTGLAAAVHLIAYYLPNYASFQLNIPRGDAVWAGFVA
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
AGMMVIFGPIAGLLSDRVGRRTMVWWSRIALLVMAYPAFVLINTFPSLTCLLVVVGCLAI
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PMAMTSPATLVLVSEVLPQRLRATGMAVTYYVAIAVFGGFAQLFSTVLIHVTGSPNAPAF
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEE
YLIGCGLVSLIGLRMVPETLGRRLS
HHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]