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The map label for this gene is thcB [H]

Identifier: 73539101

GI number: 73539101

Start: 2036393

End: 2038744

Strand: Direct

Name: thcB [H]

Synonym: Reut_B5278

Alternate gene names: 73539101

Gene position: 2036393-2038744 (Clockwise)

Preceding gene: 73539100

Following gene: 73539102

Centisome position: 74.7

GC content: 66.84

Gene sequence:

>2352_bases
ATGACCGCAGCCACCCCGGAAACCACCACGCAGAAGTGTCCGTTCAGCCATGGCGCGCAGCCCGCTGCAGCCGCGCCGAA
CGGCTGTCCGGTCAGCGCGGAGGCTGCGGCCTTCGATCCGTTTGGCGATGGCTACCAGCAGGACCCGCCCGAGTACGTGC
GCTGGTCGCGCGAGCAGGAGCCGGTGTTCTACAGCCCACAGCTCGGCTACTGGGTGGTGACGCGCTATGACGACATCAAG
GCGATTTTCCGCGACAACCTGACCTTCAGCCCCGCCATCGCGCTGGAGAAGATCACGCCGACCGGCCCAGAGGCCAACGC
CGTGCTGGCCTCGTACGGCTACGCGATGAACCGCACGCTGGTCAACGAGGACGAGCCCGCGCACATGCCGCGCCGCCGCG
CGCTGATGGAGCCGTTCACGCCCGAGCAGCTCAAGCACCATGAACCGCTGGTGCGCAAGCTGGCGCGCGAGTATGTGGAC
CGCTTCATCGACGACGGCAAGGCCGACCTCGTCGACCAGATGCTGTGGGAAGTCCCGCTGACGGTCGCCCTGCATTTCCT
CGGCGTGCCCGAGGAAGACATGGACCTGCTGCGCCAGTATTCGATCGCGCATACGGTCAACACCTGGGGCCGGCCGAAGC
CCGAAGAGCAGGTCGCGGTCGCTCATGCGGTCGGCAACTTCTGGCAGCTCGCCGGTCGCATCCTCGACAAGATGCGCGCA
GACCCGTCCGCGCCAGGCTGGATGCAATACGGCATCCAAAAGCAGAAGGAACTGCCCGAAGTCGTCACCGATTCCTACCT
GCATTCGATGATGATGGCCGGCATTGTCGCCGCGCACGAAACCACCGCGAATGCCAGCGCCAACGCCATCAAGCTGCTGC
TGCAGCATCCGGACGTGTGGCGCGAGATCTGCGAGGACCCCGCGCTGATCCCCAACGCGGTCGAGGAATGCCTGCGCCAC
AACGGCTCGGTGGCGGCATGGCGGCGCGTGGTCACGCGCGATACCGAAGTCGGCGGCATCCCCATCGCGGCCGGTTCGAA
ACTGCTGATCGTGACCTCGTCGGCGAACCATGACGAGGCGCACTTCGCCGATGCCGACCTCTTTGACATCCGCCGCGAGA
ACGCCGCCGACCAGCTCACCTTCGGCTACGGCTCGCACCAGTGCATGGGCAAGAACCTGGCCCGCATGGAGATGCAGATC
TTCCTGGAGGAACTGACGCGCCGGCTGCCGCATATGCGGCTCGCGCAGCAGTCATTCACGTATCTACCCAACACTTCGTT
CCGCGGCCCCGAGCATCTGTGGGTCGAATGGGACCCGGCGCAGAACCCGGAGCGCACGCAACCATCGTTGCTCGCGCAGC
AGACGCCGGTGCGCATTGGTGAGCCCTCAGGGCAAGCCATCAGCCGGCCCGTGGTCGTACAGAGCGTCACGCAGGCAGCG
GACGGCATCGTGCGGCTGCGGCTCGCCTCGCCCGATGGCAAGGCGATGCCGCGCTGGTCGCCGGGCTCGCATATCGATGT
CGATTGCGGCGAGACGGGCCTGTCGCGCCAGTATTCGCTGTGCGGCGACCCTGCCGAAACCGGCGTGCTCGAGATTGCCG
TGCTGCGCGAGCCGGAAAGCCGTGGCGGCTCGGCCTGGATCCACGATCACGTGAAGCCCGGCGACCGCCTGAAGATCCGC
GGCCCGCGCAATCACTTCCGCATGGATGAGGGATGCCGCAAGGCGATCTTCATTGCAGGGGGCATCGGCATCACGCCGGT
CAGCGCGATGGCGCGGCGCGCGCTGGCGCTGGGGATCGACTACGAGATCCACTACAGCGGGCGCACGCGGACCGGCATGG
CGATGATCGACGAGCTGCGCGCGCTGCATGGCGAGCGCCTGCACCTGTACATCAAGGAAGAAGGCGGCCGCAACGACCTC
GACGCGCTGCTGGCACGCACCGACCGCAACGCGCAAATCTACGCCTGCGGCCCGGCGCGCATGGTGGAAGCGCTGGAACA
GGCCTGCACCGGCTGGCCGGATGACGCGCTGCGCGTCGAACATTTCGCGACGACGCTGGGCACGCTCGATCCGTCGAAAG
AGCAGCCGTTCGAGGTAGAGCTGAAGGACTCGGGACTGACCGTCACCGTGCCCGCTGACCAGACCCTGCTGACCACGCTG
CGCTGCGCCGGCATCGACATCCAGAGCGATTGCGAGGAAGGCCTGTGCGGCTCATGCGAGGTGCGCGTGCTTTCCGGCAG
CGTCGATCATCGCGACGTCGTCCTCACGCGCGGCGAGCGCGAGGCGAACACGAAGATGATGACGTGCTGCTCGCGCGCGT
TGCCGGGTAGCCGGCTCATTCTGGAACTCTGA

Upstream 100 bases:

>100_bases
GAAACCGCGCGCTGGCAGAAACTCATCCGCGACCTGAAGATCACGCTGGACTGAGACTGGCGCCAGCCGTTGTCCACATT
GCCTTGTTGGAGCCATACCC

Downstream 100 bases:

>100_bases
GCGTGGCGGGCCCGTGTTCCGGCCTGTCCGTCCGCTTCCCCCGCTGAAACAGCAGGCCATCACCTGGCCACATGACCGCC
TGTGGGTCATGCGCCGCTTC

Product: cytochrome P450:oxidoreductase FAD/NAD(P)-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 783; Mature: 782

Protein sequence:

>783_residues
MTAATPETTTQKCPFSHGAQPAAAAPNGCPVSAEAAAFDPFGDGYQQDPPEYVRWSREQEPVFYSPQLGYWVVTRYDDIK
AIFRDNLTFSPAIALEKITPTGPEANAVLASYGYAMNRTLVNEDEPAHMPRRRALMEPFTPEQLKHHEPLVRKLAREYVD
RFIDDGKADLVDQMLWEVPLTVALHFLGVPEEDMDLLRQYSIAHTVNTWGRPKPEEQVAVAHAVGNFWQLAGRILDKMRA
DPSAPGWMQYGIQKQKELPEVVTDSYLHSMMMAGIVAAHETTANASANAIKLLLQHPDVWREICEDPALIPNAVEECLRH
NGSVAAWRRVVTRDTEVGGIPIAAGSKLLIVTSSANHDEAHFADADLFDIRRENAADQLTFGYGSHQCMGKNLARMEMQI
FLEELTRRLPHMRLAQQSFTYLPNTSFRGPEHLWVEWDPAQNPERTQPSLLAQQTPVRIGEPSGQAISRPVVVQSVTQAA
DGIVRLRLASPDGKAMPRWSPGSHIDVDCGETGLSRQYSLCGDPAETGVLEIAVLREPESRGGSAWIHDHVKPGDRLKIR
GPRNHFRMDEGCRKAIFIAGGIGITPVSAMARRALALGIDYEIHYSGRTRTGMAMIDELRALHGERLHLYIKEEGGRNDL
DALLARTDRNAQIYACGPARMVEALEQACTGWPDDALRVEHFATTLGTLDPSKEQPFEVELKDSGLTVTVPADQTLLTTL
RCAGIDIQSDCEEGLCGSCEVRVLSGSVDHRDVVLTRGEREANTKMMTCCSRALPGSRLILEL

Sequences:

>Translated_783_residues
MTAATPETTTQKCPFSHGAQPAAAAPNGCPVSAEAAAFDPFGDGYQQDPPEYVRWSREQEPVFYSPQLGYWVVTRYDDIK
AIFRDNLTFSPAIALEKITPTGPEANAVLASYGYAMNRTLVNEDEPAHMPRRRALMEPFTPEQLKHHEPLVRKLAREYVD
RFIDDGKADLVDQMLWEVPLTVALHFLGVPEEDMDLLRQYSIAHTVNTWGRPKPEEQVAVAHAVGNFWQLAGRILDKMRA
DPSAPGWMQYGIQKQKELPEVVTDSYLHSMMMAGIVAAHETTANASANAIKLLLQHPDVWREICEDPALIPNAVEECLRH
NGSVAAWRRVVTRDTEVGGIPIAAGSKLLIVTSSANHDEAHFADADLFDIRRENAADQLTFGYGSHQCMGKNLARMEMQI
FLEELTRRLPHMRLAQQSFTYLPNTSFRGPEHLWVEWDPAQNPERTQPSLLAQQTPVRIGEPSGQAISRPVVVQSVTQAA
DGIVRLRLASPDGKAMPRWSPGSHIDVDCGETGLSRQYSLCGDPAETGVLEIAVLREPESRGGSAWIHDHVKPGDRLKIR
GPRNHFRMDEGCRKAIFIAGGIGITPVSAMARRALALGIDYEIHYSGRTRTGMAMIDELRALHGERLHLYIKEEGGRNDL
DALLARTDRNAQIYACGPARMVEALEQACTGWPDDALRVEHFATTLGTLDPSKEQPFEVELKDSGLTVTVPADQTLLTTL
RCAGIDIQSDCEEGLCGSCEVRVLSGSVDHRDVVLTRGEREANTKMMTCCSRALPGSRLILEL
>Mature_782_residues
TAATPETTTQKCPFSHGAQPAAAAPNGCPVSAEAAAFDPFGDGYQQDPPEYVRWSREQEPVFYSPQLGYWVVTRYDDIKA
IFRDNLTFSPAIALEKITPTGPEANAVLASYGYAMNRTLVNEDEPAHMPRRRALMEPFTPEQLKHHEPLVRKLAREYVDR
FIDDGKADLVDQMLWEVPLTVALHFLGVPEEDMDLLRQYSIAHTVNTWGRPKPEEQVAVAHAVGNFWQLAGRILDKMRAD
PSAPGWMQYGIQKQKELPEVVTDSYLHSMMMAGIVAAHETTANASANAIKLLLQHPDVWREICEDPALIPNAVEECLRHN
GSVAAWRRVVTRDTEVGGIPIAAGSKLLIVTSSANHDEAHFADADLFDIRRENAADQLTFGYGSHQCMGKNLARMEMQIF
LEELTRRLPHMRLAQQSFTYLPNTSFRGPEHLWVEWDPAQNPERTQPSLLAQQTPVRIGEPSGQAISRPVVVQSVTQAAD
GIVRLRLASPDGKAMPRWSPGSHIDVDCGETGLSRQYSLCGDPAETGVLEIAVLREPESRGGSAWIHDHVKPGDRLKIRG
PRNHFRMDEGCRKAIFIAGGIGITPVSAMARRALALGIDYEIHYSGRTRTGMAMIDELRALHGERLHLYIKEEGGRNDLD
ALLARTDRNAQIYACGPARMVEALEQACTGWPDDALRVEHFATTLGTLDPSKEQPFEVELKDSGLTVTVPADQTLLTTLR
CAGIDIQSDCEEGLCGSCEVRVLSGSVDHRDVVLTRGEREANTKMMTCCSRALPGSRLILEL

Specific function: Degradation of thiocarbamate herbicides [H]

COG id: COG2124

COG function: function code Q; Cytochrome P450

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome P450 family [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=319, Percent_Identity=36.9905956112853, Blast_Score=191, Evalue=2e-49,
Organism=Escherichia coli, GI1787658, Length=292, Percent_Identity=26.7123287671233, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001128
- InterPro:   IPR002397
- InterPro:   IPR017972 [H]

Pfam domain/function: PF00067 p450 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 86718; Mature: 86587

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00086 CYTOCHROME_P450 ; PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAATPETTTQKCPFSHGAQPAAAAPNGCPVSAEAAAFDPFGDGYQQDPPEYVRWSREQE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCC
PVFYSPQLGYWVVTRYDDIKAIFRDNLTFSPAIALEKITPTGPEANAVLASYGYAMNRTL
CEEECCCCCEEEEEECHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHCHHHCCCC
VNEDEPAHMPRRRALMEPFTPEQLKHHEPLVRKLAREYVDRFIDDGKADLVDQMLWEVPL
CCCCCCCCCCHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TVALHFLGVPEEDMDLLRQYSIAHTVNTWGRPKPEEQVAVAHAVGNFWQLAGRILDKMRA
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
DPSAPGWMQYGIQKQKELPEVVTDSYLHSMMMAGIVAAHETTANASANAIKLLLQHPDVW
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHH
REICEDPALIPNAVEECLRHNGSVAAWRRVVTRDTEVGGIPIAAGSKLLIVTSSANHDEA
HHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCCCCC
HFADADLFDIRRENAADQLTFGYGSHQCMGKNLARMEMQIFLEELTRRLPHMRLAQQSFT
CCCCCHHHHHHHCCCCCEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
YLPNTSFRGPEHLWVEWDPAQNPERTQPSLLAQQTPVRIGEPSGQAISRPVVVQSVTQAA
CCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCEECCCCCCHHCCCHHHHHHHHHC
DGIVRLRLASPDGKAMPRWSPGSHIDVDCGETGLSRQYSLCGDPAETGVLEIAVLREPES
CCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCHHCCCCCCCCCEEEEEEEECCCC
RGGSAWIHDHVKPGDRLKIRGPRNHFRMDEGCRKAIFIAGGIGITPVSAMARRALALGID
CCCCEEEECCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHEECC
YEIHYSGRTRTGMAMIDELRALHGERLHLYIKEEGGRNDLDALLARTDRNAQIYACGPAR
EEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEEECHHH
MVEALEQACTGWPDDALRVEHFATTLGTLDPSKEQPFEVELKDSGLTVTVPADQTLLTTL
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEEECCCHHHHHHH
RCAGIDIQSDCEEGLCGSCEVRVLSGSVDHRDVVLTRGEREANTKMMTCCSRALPGSRLI
HHCCCCCHHHHCCCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEE
LEL
EEC
>Mature Secondary Structure 
TAATPETTTQKCPFSHGAQPAAAAPNGCPVSAEAAAFDPFGDGYQQDPPEYVRWSREQE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCC
PVFYSPQLGYWVVTRYDDIKAIFRDNLTFSPAIALEKITPTGPEANAVLASYGYAMNRTL
CEEECCCCCEEEEEECHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHCHHHCCCC
VNEDEPAHMPRRRALMEPFTPEQLKHHEPLVRKLAREYVDRFIDDGKADLVDQMLWEVPL
CCCCCCCCCCHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TVALHFLGVPEEDMDLLRQYSIAHTVNTWGRPKPEEQVAVAHAVGNFWQLAGRILDKMRA
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
DPSAPGWMQYGIQKQKELPEVVTDSYLHSMMMAGIVAAHETTANASANAIKLLLQHPDVW
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHH
REICEDPALIPNAVEECLRHNGSVAAWRRVVTRDTEVGGIPIAAGSKLLIVTSSANHDEA
HHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCCCCC
HFADADLFDIRRENAADQLTFGYGSHQCMGKNLARMEMQIFLEELTRRLPHMRLAQQSFT
CCCCCHHHHHHHCCCCCEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
YLPNTSFRGPEHLWVEWDPAQNPERTQPSLLAQQTPVRIGEPSGQAISRPVVVQSVTQAA
CCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCEECCCCCCHHCCCHHHHHHHHHC
DGIVRLRLASPDGKAMPRWSPGSHIDVDCGETGLSRQYSLCGDPAETGVLEIAVLREPES
CCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCHHCCCCCCCCCEEEEEEEECCCC
RGGSAWIHDHVKPGDRLKIRGPRNHFRMDEGCRKAIFIAGGIGITPVSAMARRALALGID
CCCCEEEECCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHEECC
YEIHYSGRTRTGMAMIDELRALHGERLHLYIKEEGGRNDLDALLARTDRNAQIYACGPAR
EEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEEECHHH
MVEALEQACTGWPDDALRVEHFATTLGTLDPSKEQPFEVELKDSGLTVTVPADQTLLTTL
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEEECCCHHHHHHH
RCAGIDIQSDCEEGLCGSCEVRVLSGSVDHRDVVLTRGEREANTKMMTCCSRALPGSRLI
HHCCCCCHHHHCCCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEE
LEL
EEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]