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The map label for this gene is cobB [H]

Identifier: 73539077

GI number: 73539077

Start: 2004256

End: 2005080

Strand: Reverse

Name: cobB [H]

Synonym: Reut_B5254

Alternate gene names: 73539077

Gene position: 2005080-2004256 (Counterclockwise)

Preceding gene: 73539078

Following gene: 73539076

Centisome position: 73.55

GC content: 67.27

Gene sequence:

>825_bases
TTGAGCGCGCTCGGCGATTTCGTGCGGCGCCATCCGCGCCTGTTCGTGCTGACCGGCGCGGGCATCAGCACCGATTCCGG
CATTCCCGGCTACCGCGATGCAAACGGCCAGTGGCAGCGTTCGCAGCCGATCACGCTGCAGGCCTTCCTGGGCTCGCATG
CGGGGCGCCAGCGCTATTGGGCGCGCAGCATGATCGGCTGGCCCGTGGCGGCCGGGGCGGAGCCAAATGCCGGCCATCAC
GCATTGGTGACGCTGGCGCGGCAAGGGCATGTGATGCGTCTGGTCACGCAGAACGTGGACGGGCTGCACCAGCGTGCCGG
CAGTGAGGATGTGATCGAGCTGCACGGCAGCATCGGCAGTGCAATCTGCCTGTCGTGCGGCACGCGCCATGACCGCGCCG
GCTTGCAGCGCTGGCTCACCGAGCAGAACGGCGAATTGCGCGATGTGATTGCCGAACCTGCGGCCGATGGCGATGCCCAC
TTCGAATCACCGCTGTTCGCGCATTTCCGTGTGCCGTCGTGCGAGCGGTGCGACGGTCTGCTCAAGCCCGACGTGGTGTT
CTTCGGCGAATCCGTACCGCGCGAGCGCGTCGATGGCGCACGCGAAGCCCTGGTGCAGTCGGACGCGGTGCTCGTGGTCG
GCTCGTCGCTGACGGTGTATTCGGGCTATCGCTTCTGCGTGTGGGCCGAGCAGATGGGCAAGCCCGTGGCCGTGGTCAAC
CTCGGCGTGACGCGAGCCGATCCGATGCTCACGCTCAAGGTCGAGCAGCCATGCGCGCAGGCGTTGTCCGAACTGACGAC
ATGGCTCGCTCAGCCCGGTGATTGA

Upstream 100 bases:

>100_bases
TCGATTACTACTATCAGGAAATGCAGCGATACCGCGATGAACTGAAGGCCTGGGAAGCGCGCCAGGCCGCACGCCAGTCC
GCCGCCGAGGATTCGCCCAC

Downstream 100 bases:

>100_bases
CCGGCACGCATTCGCCCGTTAGCATCGCTTCATTCGTCTTATCGTTTTCCACGCTTCCGACATCATGAACGCAGCCCGCC
ATCGCCACGCCCAGCCAGCC

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSALGDFVRRHPRLFVLTGAGISTDSGIPGYRDANGQWQRSQPITLQAFLGSHAGRQRYWARSMIGWPVAAGAEPNAGHH
ALVTLARQGHVMRLVTQNVDGLHQRAGSEDVIELHGSIGSAICLSCGTRHDRAGLQRWLTEQNGELRDVIAEPAADGDAH
FESPLFAHFRVPSCERCDGLLKPDVVFFGESVPRERVDGAREALVQSDAVLVVGSSLTVYSGYRFCVWAEQMGKPVAVVN
LGVTRADPMLTLKVEQPCAQALSELTTWLAQPGD

Sequences:

>Translated_274_residues
MSALGDFVRRHPRLFVLTGAGISTDSGIPGYRDANGQWQRSQPITLQAFLGSHAGRQRYWARSMIGWPVAAGAEPNAGHH
ALVTLARQGHVMRLVTQNVDGLHQRAGSEDVIELHGSIGSAICLSCGTRHDRAGLQRWLTEQNGELRDVIAEPAADGDAH
FESPLFAHFRVPSCERCDGLLKPDVVFFGESVPRERVDGAREALVQSDAVLVVGSSLTVYSGYRFCVWAEQMGKPVAVVN
LGVTRADPMLTLKVEQPCAQALSELTTWLAQPGD
>Mature_273_residues
SALGDFVRRHPRLFVLTGAGISTDSGIPGYRDANGQWQRSQPITLQAFLGSHAGRQRYWARSMIGWPVAAGAEPNAGHHA
LVTLARQGHVMRLVTQNVDGLHQRAGSEDVIELHGSIGSAICLSCGTRHDRAGLQRWLTEQNGELRDVIAEPAADGDAHF
ESPLFAHFRVPSCERCDGLLKPDVVFFGESVPRERVDGAREALVQSDAVLVVGSSLTVYSGYRFCVWAEQMGKPVAVVNL
GVTRADPMLTLKVEQPCAQALSELTTWLAQPGD

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI6912662, Length=265, Percent_Identity=43.0188679245283, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI300795542, Length=270, Percent_Identity=30.7407407407407, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI6912664, Length=273, Percent_Identity=30.7692307692308, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI13787215, Length=255, Percent_Identity=31.3725490196078, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI13775602, Length=216, Percent_Identity=29.6296296296296, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI13775600, Length=216, Percent_Identity=29.6296296296296, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI300797705, Length=217, Percent_Identity=29.4930875576037, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI6912660, Length=216, Percent_Identity=30.0925925925926, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI63054862, Length=212, Percent_Identity=30.6603773584906, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI7706712, Length=274, Percent_Identity=29.5620437956204, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI308199517, Length=274, Percent_Identity=28.8321167883212, Blast_Score=86, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71990482, Length=259, Percent_Identity=40.1544401544402, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17567771, Length=259, Percent_Identity=38.6100386100386, Blast_Score=187, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI71990487, Length=261, Percent_Identity=39.8467432950192, Blast_Score=187, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17541892, Length=261, Percent_Identity=26.0536398467433, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17505510, Length=121, Percent_Identity=35.5371900826446, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28571445, Length=271, Percent_Identity=43.9114391143911, Blast_Score=218, Evalue=3e-57,
Organism=Drosophila melanogaster, GI28571443, Length=220, Percent_Identity=43.6363636363636, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI28571441, Length=220, Percent_Identity=43.6363636363636, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24648389, Length=216, Percent_Identity=26.3888888888889, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24650933, Length=286, Percent_Identity=28.3216783216783, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 29731; Mature: 29600

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALGDFVRRHPRLFVLTGAGISTDSGIPGYRDANGQWQRSQPITLQAFLGSHAGRQRYW
CCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHCCCCCHHHHH
ARSMIGWPVAAGAEPNAGHHALVTLARQGHVMRLVTQNVDGLHQRAGSEDVIELHGSIGS
HHHHCCCCEECCCCCCCCCCEEEEECCCCCEEEEEHHCHHHHHHHCCCCCCEEECCCCCH
AICLSCGTRHDRAGLQRWLTEQNGELRDVIAEPAADGDAHFESPLFAHFRVPSCERCDGL
HHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHCCC
LKPDVVFFGESVPRERVDGAREALVQSDAVLVVGSSLTVYSGYRFCVWAEQMGKPVAVVN
CCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCEEEECCCEEEEEHHHHCCCEEEEE
LGVTRADPMLTLKVEQPCAQALSELTTWLAQPGD
ECCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SALGDFVRRHPRLFVLTGAGISTDSGIPGYRDANGQWQRSQPITLQAFLGSHAGRQRYW
CHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHCCCCCHHHHH
ARSMIGWPVAAGAEPNAGHHALVTLARQGHVMRLVTQNVDGLHQRAGSEDVIELHGSIGS
HHHHCCCCEECCCCCCCCCCEEEEECCCCCEEEEEHHCHHHHHHHCCCCCCEEECCCCCH
AICLSCGTRHDRAGLQRWLTEQNGELRDVIAEPAADGDAHFESPLFAHFRVPSCERCDGL
HHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHCCC
LKPDVVFFGESVPRERVDGAREALVQSDAVLVVGSSLTVYSGYRFCVWAEQMGKPVAVVN
CCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCEEEECCCEEEEEHHHHCCCEEEEE
LGVTRADPMLTLKVEQPCAQALSELTTWLAQPGD
ECCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA