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The map label for this gene is amnF [H]

Identifier: 73539043

GI number: 73539043

Start: 1967663

End: 1968457

Strand: Reverse

Name: amnF [H]

Synonym: Reut_B5220

Alternate gene names: 73539043

Gene position: 1968457-1967663 (Counterclockwise)

Preceding gene: 73539044

Following gene: 73539041

Centisome position: 72.21

GC content: 65.41

Gene sequence:

>795_bases
ATGAAGATGGACACCACGATCCTGCAGACACTGGCTGTATCGCTGCGCCATGCCGCGCAGCAGAACCAGCCGATTCCGCC
CGTGCGGGACGCCCTGTCCGCCGTGGGCGTGCAAGGCGCCTATACGGTACAGGACATCAATACCGCGTACTACCTCGACA
CCGGCCGCCGGCTGACGGGCCGCAAGATCGGCCTGACGTCGAAGTCCGTGCAGCAGCAGCTTGGCGTGGATGCGCCCGAT
TTCGGCATGCTGTTCGCGGACATGGAACTCGCTCCAGACGAAGAGGTTGCGCTCGACCGCGTGTTGCAACCGAAGGTCGA
GGCGGAAGTGGCCGTCGTGCTGGAGCGCGACCTGAAGCACGAACAGGTCACGCTGTCGCAGTTGATCTCCGCGATTGCTT
ATGTGTTGCCGGCCGTGGAGATTGTCGGCAGCCGCATCGCAAATTGGGACATCCGCCTGCTGGACACCGTGGCGGACAAC
GCGTCGTCTGGCCTGTATGCGCTCGGGACGCAGCCGCGCAGGCTGTCGGAGGTGGATCTGCGCATGTGCGGCATGCTCAT
GGAGCGCAAGGGTGAGGTGGTATCGCTCGGCGTGGGCGCCGCGTGCCTGAGCCATCCGCTCAACGCTGCGCTGTGGCTGG
CTCGCAAGATGGCGGAAGTGGGAAGGCCGTTGCTTGCGGGCGACGTCATCATGACGGGCGCGCTCGGGCCCATGGTGACC
GTCGCGCCGGGCGACATCATCGACACCACCATCGCGGGTCTTGGCTCGGTCCGCACTGCGTTTGCCGCTGACTGA

Upstream 100 bases:

>100_bases
CCGTTCGCGAAAGCGTCGCTGTTCCGAAGCGTTGTCATGAAGCCCTGGAAGCTCGTGTTCGCGAGCCAGGACAACCTGAA
TCTCCCGCTGTGAGGCGCAT

Downstream 100 bases:

>100_bases
ATCGAGCGATTGAGATTAACGCGAAAGCCCGTGCGGGCTCAGGCCCGTGTCAGCTTTCGGATCAGGTCGATGACGGTCCG
GCTCGCATACGTCAGCGGCC

Product: 4-oxalocrotonate decarboxylase

Products: NA

Alternate protein names: 2-keto-4-pentenoate hydratase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKMDTTILQTLAVSLRHAAQQNQPIPPVRDALSAVGVQGAYTVQDINTAYYLDTGRRLTGRKIGLTSKSVQQQLGVDAPD
FGMLFADMELAPDEEVALDRVLQPKVEAEVAVVLERDLKHEQVTLSQLISAIAYVLPAVEIVGSRIANWDIRLLDTVADN
ASSGLYALGTQPRRLSEVDLRMCGMLMERKGEVVSLGVGAACLSHPLNAALWLARKMAEVGRPLLAGDVIMTGALGPMVT
VAPGDIIDTTIAGLGSVRTAFAAD

Sequences:

>Translated_264_residues
MKMDTTILQTLAVSLRHAAQQNQPIPPVRDALSAVGVQGAYTVQDINTAYYLDTGRRLTGRKIGLTSKSVQQQLGVDAPD
FGMLFADMELAPDEEVALDRVLQPKVEAEVAVVLERDLKHEQVTLSQLISAIAYVLPAVEIVGSRIANWDIRLLDTVADN
ASSGLYALGTQPRRLSEVDLRMCGMLMERKGEVVSLGVGAACLSHPLNAALWLARKMAEVGRPLLAGDVIMTGALGPMVT
VAPGDIIDTTIAGLGSVRTAFAAD
>Mature_264_residues
MKMDTTILQTLAVSLRHAAQQNQPIPPVRDALSAVGVQGAYTVQDINTAYYLDTGRRLTGRKIGLTSKSVQQQLGVDAPD
FGMLFADMELAPDEEVALDRVLQPKVEAEVAVVLERDLKHEQVTLSQLISAIAYVLPAVEIVGSRIANWDIRLLDTVADN
ASSGLYALGTQPRRLSEVDLRMCGMLMERKGEVVSLGVGAACLSHPLNAALWLARKMAEVGRPLLAGDVIMTGALGPMVT
VAPGDIIDTTIAGLGSVRTAFAAD

Specific function: Involved in the modified meta-cleavage pathway for the 2-aminophenol catabolism [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=261, Percent_Identity=52.1072796934866, Blast_Score=280, Evalue=8e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =4.2.1.80 [H]

Molecular weight: Translated: 28172; Mature: 28172

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMDTTILQTLAVSLRHAAQQNQPIPPVRDALSAVGVQGAYTVQDINTAYYLDTGRRLTG
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCEEEEEECCCEECC
RKIGLTSKSVQQQLGVDAPDFGMLFADMELAPDEEVALDRVLQPKVEAEVAVVLERDLKH
CEECCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCH
EQVTLSQLISAIAYVLPAVEIVGSRIANWDIRLLDTVADNASSGLYALGTQPRRLSEVDL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCEEEECCCCCHHHHHHH
RMCGMLMERKGEVVSLGVGAACLSHPLNAALWLARKMAEVGRPLLAGDVIMTGALGPMVT
HHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHHCCCCCEEE
VAPGDIIDTTIAGLGSVRTAFAAD
ECCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKMDTTILQTLAVSLRHAAQQNQPIPPVRDALSAVGVQGAYTVQDINTAYYLDTGRRLTG
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCEEEEEECCCEECC
RKIGLTSKSVQQQLGVDAPDFGMLFADMELAPDEEVALDRVLQPKVEAEVAVVLERDLKH
CEECCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCH
EQVTLSQLISAIAYVLPAVEIVGSRIANWDIRLLDTVADNASSGLYALGTQPRRLSEVDL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCEEEECCCCCHHHHHHH
RMCGMLMERKGEVVSLGVGAACLSHPLNAALWLARKMAEVGRPLLAGDVIMTGALGPMVT
HHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHHCCCCCEEE
VAPGDIIDTTIAGLGSVRTAFAAD
ECCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9169437; 11081795 [H]