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The map label for this gene is yneJ [H]

Identifier: 73538952

GI number: 73538952

Start: 1868456

End: 1869343

Strand: Direct

Name: yneJ [H]

Synonym: Reut_B5127

Alternate gene names: 73538952

Gene position: 1868456-1869343 (Clockwise)

Preceding gene: 73538951

Following gene: 73538953

Centisome position: 68.54

GC content: 65.65

Gene sequence:

>888_bases
GTGGAACTCGCAGAACTGGAAATCTTTCGCGCCGTCGCGCGCGAACAGAGCATCACCCGGGCAGCAAAGGCCCTCGCGCG
CGTGCAGTCCAACGTCACGACGCGGGTCAAGCAGCTCGAGGAGAGCCTTGGCGTCACCCTGTTCCAGCGCGACAGCAGGA
AGATGATCCTGACGCCGGAAGGGCAGCGGCTGCTCGGCTACGCGGAACAGATGCTGACGCTGGCCGAGGAAGCGCGGCAA
TCCATGCGCCTCAGTGCGCCAAGCGGCACGCTGCGGCTCGGCTCGATGGAGAGTTCTGCCGCAACGTTGCTGCCTAAGCC
GCTGGCTCAGTTCCATGCCGCATGGCCTGAAGTTGAACTGAAGCTGACCACGGGAACCACGGGGTTTCTCGTCGATGCCG
TGCTGGGCCATCGGCTGGACTGTGCAGTGGTTGCGCATCCGGGCACCGGCACGCCACGCAATACGAGCATGGCGGCGCTT
GGCGAGGGACTGGAAGGCGTTTTCCTGCGGACCGAGGAGCTGGTGCTGATCCTGCCCGGCACGCACACAAGGGCGCGCGG
CCCGCGGGACGTGACGCTACGGCATCTCGCCGCGTTCTCGAAAGGCTGCACTTATCGCCGCTGCGCGGAAGACTGGCTCG
AGCAACGTGGCGAGGATCTCCGCCACCGGCTGTCGGTGATCGAAATGCCGTCGTATCACGCGATCCTGGCCTGCGTCACC
GCCGGATCGGCCGCGGCGATTGTGCCGCGCTCGCTGCTTGCCATGTACCCCGATGCCACCGATCTGCAGACCGTGCCGGT
GCGTTCCGCACACACCTTCCTGATCAGGCGTGCGGGCTTCACGACCTCGGCATACGAAGCGTTCGTGCAGGAACTGCGCC
ATACCTGA

Upstream 100 bases:

>100_bases
CTGACACCTGCTTCCTTGACACATTGTTCTCAATTTACAATACTCAATACACTTTCATTCTTAATTAGAGAACGCTTCGC
CAAGCGTCGCGCCCGGCCAC

Downstream 100 bases:

>100_bases
CACACGGGATGGCACAGCGACCGCAGGAATTTGAAACCACTACCCGACCTCAACAGGAATCACTGTCATGGACACCACCA
CGCGTTCCGGCGATGGCCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MELAELEIFRAVAREQSITRAAKALARVQSNVTTRVKQLEESLGVTLFQRDSRKMILTPEGQRLLGYAEQMLTLAEEARQ
SMRLSAPSGTLRLGSMESSAATLLPKPLAQFHAAWPEVELKLTTGTTGFLVDAVLGHRLDCAVVAHPGTGTPRNTSMAAL
GEGLEGVFLRTEELVLILPGTHTRARGPRDVTLRHLAAFSKGCTYRRCAEDWLEQRGEDLRHRLSVIEMPSYHAILACVT
AGSAAAIVPRSLLAMYPDATDLQTVPVRSAHTFLIRRAGFTTSAYEAFVQELRHT

Sequences:

>Translated_295_residues
MELAELEIFRAVAREQSITRAAKALARVQSNVTTRVKQLEESLGVTLFQRDSRKMILTPEGQRLLGYAEQMLTLAEEARQ
SMRLSAPSGTLRLGSMESSAATLLPKPLAQFHAAWPEVELKLTTGTTGFLVDAVLGHRLDCAVVAHPGTGTPRNTSMAAL
GEGLEGVFLRTEELVLILPGTHTRARGPRDVTLRHLAAFSKGCTYRRCAEDWLEQRGEDLRHRLSVIEMPSYHAILACVT
AGSAAAIVPRSLLAMYPDATDLQTVPVRSAHTFLIRRAGFTTSAYEAFVQELRHT
>Mature_295_residues
MELAELEIFRAVAREQSITRAAKALARVQSNVTTRVKQLEESLGVTLFQRDSRKMILTPEGQRLLGYAEQMLTLAEEARQ
SMRLSAPSGTLRLGSMESSAATLLPKPLAQFHAAWPEVELKLTTGTTGFLVDAVLGHRLDCAVVAHPGTGTPRNTSMAAL
GEGLEGVFLRTEELVLILPGTHTRARGPRDVTLRHLAAFSKGCTYRRCAEDWLEQRGEDLRHRLSVIEMPSYHAILACVT
AGSAAAIVPRSLLAMYPDATDLQTVPVRSAHTFLIRRAGFTTSAYEAFVQELRHT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=296, Percent_Identity=36.8243243243243, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI87082132, Length=281, Percent_Identity=27.4021352313167, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788626, Length=146, Percent_Identity=32.1917808219178, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790208, Length=266, Percent_Identity=27.4436090225564, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788481, Length=196, Percent_Identity=30.6122448979592, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787589, Length=144, Percent_Identity=34.0277777777778, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788748, Length=151, Percent_Identity=30.4635761589404, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI157672245, Length=293, Percent_Identity=25.938566552901, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788887, Length=186, Percent_Identity=30.1075268817204, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI145693105, Length=152, Percent_Identity=28.2894736842105, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788706, Length=128, Percent_Identity=34.375, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1789173, Length=120, Percent_Identity=32.5, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1790399, Length=149, Percent_Identity=30.2013422818792, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787879, Length=263, Percent_Identity=28.5171102661597, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787128, Length=153, Percent_Identity=32.0261437908497, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788296, Length=130, Percent_Identity=32.3076923076923, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790262, Length=128, Percent_Identity=32.8125, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786401, Length=157, Percent_Identity=31.2101910828025, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32374; Mature: 32374

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELAELEIFRAVAREQSITRAAKALARVQSNVTTRVKQLEESLGVTLFQRDSRKMILTPE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCEEEECCC
GQRLLGYAEQMLTLAEEARQSMRLSAPSGTLRLGSMESSAATLLPKPLAQFHAAWPEVEL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHCCHHHHHHHCCCCCEEE
KLTTGTTGFLVDAVLGHRLDCAVVAHPGTGTPRNTSMAALGEGLEGVFLRTEELVLILPG
EEECCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHCCCCEEEEEECCEEEEECC
THTRARGPRDVTLRHLAAFSKGCTYRRCAEDWLEQRGEDLRHRLSVIEMPSYHAILACVT
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
AGSAAAIVPRSLLAMYPDATDLQTVPVRSAHTFLIRRAGFTTSAYEAFVQELRHT
CCCHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MELAELEIFRAVAREQSITRAAKALARVQSNVTTRVKQLEESLGVTLFQRDSRKMILTPE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCEEEECCC
GQRLLGYAEQMLTLAEEARQSMRLSAPSGTLRLGSMESSAATLLPKPLAQFHAAWPEVEL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHCCHHHHHHHCCCCCEEE
KLTTGTTGFLVDAVLGHRLDCAVVAHPGTGTPRNTSMAALGEGLEGVFLRTEELVLILPG
EEECCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHCCCCEEEEEECCEEEEECC
THTRARGPRDVTLRHLAAFSKGCTYRRCAEDWLEQRGEDLRHRLSVIEMPSYHAILACVT
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
AGSAAAIVPRSLLAMYPDATDLQTVPVRSAHTFLIRRAGFTTSAYEAFVQELRHT
CCCHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]