BacMap

Definition	Ralstonia eutropha JMP134 chromosome chromosome 2, complete sequence.
Accession	NC_007348
Length	2,726,152

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The map label for this gene is dkgB [H]

Identifier: 73538941

GI number: 73538941

Start: 1857331

End: 1858134

Strand: Reverse

Name: dkgB [H]

Synonym: Reut_B5116

Alternate gene names: 73538941

Gene position: 1858134-1857331 (Counterclockwise)

Preceding gene: 73538944

Following gene: 73538940

Centisome position: 68.16

GC content: 65.67

Gene sequence:

>804_bases
ATGAGCATTCCTTCTTTCGGCGTCGGTACGTTCCGCCTGACCGGCCAAGCCGCCGTCGACTCCGTATACAACGCGCTGGA
CCTCGGCTACCGCGCCATCGACACTGCGCAGATCTACGGCAACGAGGCCGATGTCGGCCAGGCCATCGCCGAAAGCGGCG
TGAAACGCGACGAGCTGTTCCTCACCACCAAGATCTGGACCGAGCACTACGCCGCCGACAAGCTCGTGCCGAGCCTGCGC
GAAAGCCTGGACAAGCTGCGCACCGACTATGTCGACCTGACGCTGATCCACTGGCCCGCGCCGGGCAACGGCGTGCCGCT
GCGCGAGTTCATGGAGGCGCTCGCCGCGGCGAAGTCGCTGGGGCTGACGCGCCAGATCGGCGTCTCGAACTTCAATATCG
CGCTGACGCACCAGGCCATCGACGTGGTCGGCAAGGGAGAGATCGCGACCAACCAGATCGAACTGAGCCCCTACCTGCAG
AGCCGCAAGCTGACGGCGTTCCTGACGGAGCAGGGCATCGCAGTGACGTCGTACATGACGCTGGCTTACGGCAAGGTGCT
GACGGACCCTGTGCTCGCACAGATCGCCGCAAAGCATGGCGCTACGGTGGCACAGGTTGCCCTCGCCTGGGCGCTGCAGC
TCGGCTATGCCGTGATCCCGTCGTCGACGAAGCGCGAGAACCTCGCAAGCAACCTGCTGGCGCGCGACCTGAAGCTCGAC
GCCGGCGACATGGCGCAGATCACCGCGCTGGAACGCAACGGCCGCGAAGTCAACCCCGAGGGTCTGGCGCCGGAATGGGA
CTGA

Upstream 100 bases:

>100_bases
TCAAATGAATTTGACTTATACACGGCTTAATTTGCAAATGTAAAGCCGGCAAACTGGCTCCATTCCAAGTTATCTCTTGC
TGCCACACAGGAGTCCCCTC

Downstream 100 bases:

>100_bases
GGCCAGGCTGCTGCATTCGACATTTCATGTCGCCACGAAGCTTCGGGGCAGTGGACAGTGCAAAGATTCGTCTGACGCTG
TAGCGCCGCGAGCATTCCTG

Product: 2,5-diketo-D-gluconate reductase B

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSIPSFGVGTFRLTGQAAVDSVYNALDLGYRAIDTAQIYGNEADVGQAIAESGVKRDELFLTTKIWTEHYAADKLVPSLR
ESLDKLRTDYVDLTLIHWPAPGNGVPLREFMEALAAAKSLGLTRQIGVSNFNIALTHQAIDVVGKGEIATNQIELSPYLQ
SRKLTAFLTEQGIAVTSYMTLAYGKVLTDPVLAQIAAKHGATVAQVALAWALQLGYAVIPSSTKRENLASNLLARDLKLD
AGDMAQITALERNGREVNPEGLAPEWD

Sequences:

>Translated_267_residues
MSIPSFGVGTFRLTGQAAVDSVYNALDLGYRAIDTAQIYGNEADVGQAIAESGVKRDELFLTTKIWTEHYAADKLVPSLR
ESLDKLRTDYVDLTLIHWPAPGNGVPLREFMEALAAAKSLGLTRQIGVSNFNIALTHQAIDVVGKGEIATNQIELSPYLQ
SRKLTAFLTEQGIAVTSYMTLAYGKVLTDPVLAQIAAKHGATVAQVALAWALQLGYAVIPSSTKRENLASNLLARDLKLD
AGDMAQITALERNGREVNPEGLAPEWD
>Mature_266_residues
SIPSFGVGTFRLTGQAAVDSVYNALDLGYRAIDTAQIYGNEADVGQAIAESGVKRDELFLTTKIWTEHYAADKLVPSLRE
SLDKLRTDYVDLTLIHWPAPGNGVPLREFMEALAAAKSLGLTRQIGVSNFNIALTHQAIDVVGKGEIATNQIELSPYLQS
RKLTAFLTEQGIAVTSYMTLAYGKVLTDPVLAQIAAKHGATVAQVALAWALQLGYAVIPSSTKRENLASNLLARDLKLDA
GDMAQITALERNGREVNPEGLAPEWD

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI310109922, Length=272, Percent_Identity=33.4558823529412, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI310109920, Length=289, Percent_Identity=33.9100346020761, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI45446745, Length=289, Percent_Identity=33.9100346020761, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI4503285, Length=289, Percent_Identity=33.9100346020761, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI223468663, Length=289, Percent_Identity=33.2179930795848, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI5453543, Length=289, Percent_Identity=33.5640138408304, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI24497585, Length=291, Percent_Identity=33.3333333333333, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI24497577, Length=292, Percent_Identity=31.5068493150685, Blast_Score=147, Evalue=6e-36,
Organism=Homo sapiens, GI5174391, Length=292, Percent_Identity=31.5068493150685, Blast_Score=147, Evalue=6e-36,
Organism=Homo sapiens, GI24497583, Length=297, Percent_Identity=33.3333333333333, Blast_Score=147, Evalue=8e-36,
Organism=Homo sapiens, GI5174695, Length=293, Percent_Identity=32.7645051194539, Blast_Score=144, Evalue=7e-35,
Organism=Homo sapiens, GI93277124, Length=298, Percent_Identity=30.5369127516779, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI4502049, Length=286, Percent_Identity=35.6643356643357, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI291291012, Length=268, Percent_Identity=33.2089552238806, Blast_Score=138, Evalue=5e-33,
Organism=Homo sapiens, GI300116273, Length=268, Percent_Identity=32.4626865671642, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI300116271, Length=279, Percent_Identity=30.4659498207885, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI310109924, Length=154, Percent_Identity=32.4675324675325, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI207028673, Length=104, Percent_Identity=38.4615384615385, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI310109926, Length=104, Percent_Identity=38.4615384615385, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=66.6666666666667, Blast_Score=363, Evalue=1e-102,
Organism=Escherichia coli, GI87082198, Length=263, Percent_Identity=35.7414448669202, Blast_Score=167, Evalue=6e-43,
Organism=Escherichia coli, GI1788081, Length=268, Percent_Identity=29.4776119402985, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI48994888, Length=270, Percent_Identity=29.6296296296296, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787674, Length=281, Percent_Identity=26.3345195729537, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87081735, Length=292, Percent_Identity=26.7123287671233, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17537077, Length=292, Percent_Identity=34.9315068493151, Blast_Score=147, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17561298, Length=275, Percent_Identity=31.6363636363636, Blast_Score=144, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17537075, Length=293, Percent_Identity=34.8122866894198, Blast_Score=144, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI71998625, Length=262, Percent_Identity=32.4427480916031, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17550248, Length=285, Percent_Identity=33.6842105263158, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17537079, Length=297, Percent_Identity=31.6498316498317, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17566692, Length=286, Percent_Identity=31.8181818181818, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17552492, Length=268, Percent_Identity=29.8507462686567, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17564128, Length=292, Percent_Identity=32.1917808219178, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17561300, Length=273, Percent_Identity=28.2051282051282, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17538386, Length=267, Percent_Identity=30.3370786516854, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17562292, Length=271, Percent_Identity=29.520295202952, Blast_Score=107, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6320576, Length=280, Percent_Identity=36.4285714285714, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324694, Length=261, Percent_Identity=36.0153256704981, Blast_Score=145, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6322556, Length=252, Percent_Identity=30.952380952381, Blast_Score=135, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6321896, Length=306, Percent_Identity=30.3921568627451, Blast_Score=134, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319625, Length=269, Percent_Identity=29.7397769516729, Blast_Score=96, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6320079, Length=248, Percent_Identity=31.4516129032258, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24662789, Length=288, Percent_Identity=33.6805555555556, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24657054, Length=287, Percent_Identity=34.1463414634146, Blast_Score=148, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24663317, Length=288, Percent_Identity=33.6805555555556, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24644950, Length=295, Percent_Identity=33.5593220338983, Blast_Score=140, Evalue=8e-34,
Organism=Drosophila melanogaster, GI20129731, Length=286, Percent_Identity=32.1678321678322, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI221378297, Length=315, Percent_Identity=32.3809523809524, Blast_Score=134, Evalue=4e-32,
Organism=Drosophila melanogaster, GI21356425, Length=275, Percent_Identity=31.6363636363636, Blast_Score=130, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24662785, Length=290, Percent_Identity=33.448275862069, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI45553081, Length=287, Percent_Identity=29.616724738676, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24662781, Length=290, Percent_Identity=32.7586206896552, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI281366140, Length=261, Percent_Identity=29.5019157088123, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 28841; Mature: 28710

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPSFGVGTFRLTGQAAVDSVYNALDLGYRAIDTAQIYGNEADVGQAIAESGVKRDELF
CCCCCCCCCEEEEECHHHHHHHHHHHHHCHHHEEHHHHCCCCHHHHHHHHHCCCCCHHEE
LTTKIWTEHYAADKLVPSLRESLDKLRTDYVDLTLIHWPAPGNGVPLREFMEALAAAKSL
EEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHC
GLTRQIGVSNFNIALTHQAIDVVGKGEIATNQIELSPYLQSRKLTAFLTEQGIAVTSYMT
CCHHCCCCCCCEEEEEEHHHHHEECCCEECCEEEECHHHHCCCCEEEECCCCCCHHHHHH
LAYGKVLTDPVLAQIAAKHGATVAQVALAWALQLGYAVIPSSTKRENLASNLLARDLKLD
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHCCC
AGDMAQITALERNGREVNPEGLAPEWD
CCCHHHEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SIPSFGVGTFRLTGQAAVDSVYNALDLGYRAIDTAQIYGNEADVGQAIAESGVKRDELF
CCCCCCCCEEEEECHHHHHHHHHHHHHCHHHEEHHHHCCCCHHHHHHHHHCCCCCHHEE
LTTKIWTEHYAADKLVPSLRESLDKLRTDYVDLTLIHWPAPGNGVPLREFMEALAAAKSL
EEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHC
GLTRQIGVSNFNIALTHQAIDVVGKGEIATNQIELSPYLQSRKLTAFLTEQGIAVTSYMT
CCHHCCCCCCCEEEEEEHHHHHEECCCEECCEEEECHHHHCCCCEEEECCCCCCHHHHHH
LAYGKVLTDPVLAQIAAKHGATVAQVALAWALQLGYAVIPSSTKRENLASNLLARDLKLD
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHCCC
AGDMAQITALERNGREVNPEGLAPEWD
CCCHHHEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]