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The map label for this gene is phaZ1 [H]

Identifier: 73538938

GI number: 73538938

Start: 1854465

End: 1855577

Strand: Reverse

Name: phaZ1 [H]

Synonym: Reut_B5113

Alternate gene names: 73538938

Gene position: 1855577-1854465 (Counterclockwise)

Preceding gene: 73538939

Following gene: 73538935

Centisome position: 68.07

GC content: 69.54

Gene sequence:

>1113_bases
ATGGGACGCCGCGGCGGCAGCACGGCACGGCTATGGACAGCGTTGGGCAAGAGCGCGACGCGCCAGGCCCGGCGCATGGG
GCGCGAGATGACCCGCGCGATCAACAAGTCGGTGACCAAGCCGATGACCGAGGCCATCGTGCGCAACGCGGTGAAGCAGT
CATCGGCCGTCACCAAGGCCACGCAACGCGCGCTGTCCGGGGTGGCCTCGCCCGTGCCGCCGCCACAAAGCCGCGGCAGC
GGACGTTGGGAAGAAGGCGTTGGCGGACTCGGCCCGCTCGCGCAGCGCAACTACCGCATCTTCATACCGGCCGGCGTAAC
GGCCCGGCGGCGTGCGCCGATGCTCGTGCTCTTGCACGGCTGCGGCCAGGACGCCGCGAGCTTTGCCACGGTCACGCGCG
CTGTAACCGTGGCGCGGGAGGCGGGCTGCGTCGTGCTGCTGCCGGAGCAGTCGTCGCAGGGCAACGCACAACGGTGCTGG
AACTGGTTTCGTCCCGCTACGCGCGGGAGTGCCGAGGCCGCGTTGCTGATGGCGATGATCGACCATGTGTGTGGCCGCTA
TCCGGTGCGGCCTGACCGCGTCAGCGTGATGGGGCTATCGGCCGGCGGTGCGATGGCGATGATGCTGGGATTGCGTTATC
CGGATCGCTTTATCGGAGTTAGCTCGCATGCGGGGGCGGTGCCTTACAGCGCTGGCAACACGGCGCAGGCCGGGGCGGCC
ATGCGGGGCAGCCGGGTGCCGGATGCTGCAGCCCGGCAGGCACTGCGGTTATGGTTGGCGGGGCGGCCTTTGCCGCCGTT
GCTGCTGCTGCATGGCGATAGGGATTCGGTGGTTTCGGTGGAGAACGCGATGACGGCTGCGCGGCTTTGGCTGGACTTGC
TGCCGGATCCGTCGGTGCGGCTGGTCGTGCCTGGCGCGCGTCGCGTGCAGCGTGGGCAAAGGTTGCCGGCGGAGGTGTCT
GACTGGCTGGTCGACGGGGAAATCTTCGTGCGGCTCGTCAGGATCGAGGGCCTCGGGCATGCGTGGAGCGGGGGGCCGGC
GGCGCAGGCCTTTTCCGATCCGCGGGGACCGGATGCGTTGCGGATCGCGTTGCGGTTTTTCGGGGACAGGTGA

Upstream 100 bases:

>100_bases
CCGCGCAACGCGCTGAGCCGCCGTGGCTCGCGCATACGCCTGACAAGAAAGCCTGACACAATCGATCCCGTCAACGGAAT
CCGGACGGGAGGCCAGTCAC

Downstream 100 bases:

>100_bases
GGGCAAGCCTACTCGAGCTTGCCCGGCTCCATCACGACATCGCTGACGGGACACGCCACACAAGGCAGGATCCACCTGTC
ATCCTTCTCATCCGGGCTGA

Product: esterase, PHB depolymerase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MGRRGGSTARLWTALGKSATRQARRMGREMTRAINKSVTKPMTEAIVRNAVKQSSAVTKATQRALSGVASPVPPPQSRGS
GRWEEGVGGLGPLAQRNYRIFIPAGVTARRRAPMLVLLHGCGQDAASFATVTRAVTVAREAGCVVLLPEQSSQGNAQRCW
NWFRPATRGSAEAALLMAMIDHVCGRYPVRPDRVSVMGLSAGGAMAMMLGLRYPDRFIGVSSHAGAVPYSAGNTAQAGAA
MRGSRVPDAAARQALRLWLAGRPLPPLLLLHGDRDSVVSVENAMTAARLWLDLLPDPSVRLVVPGARRVQRGQRLPAEVS
DWLVDGEIFVRLVRIEGLGHAWSGGPAAQAFSDPRGPDALRIALRFFGDR

Sequences:

>Translated_370_residues
MGRRGGSTARLWTALGKSATRQARRMGREMTRAINKSVTKPMTEAIVRNAVKQSSAVTKATQRALSGVASPVPPPQSRGS
GRWEEGVGGLGPLAQRNYRIFIPAGVTARRRAPMLVLLHGCGQDAASFATVTRAVTVAREAGCVVLLPEQSSQGNAQRCW
NWFRPATRGSAEAALLMAMIDHVCGRYPVRPDRVSVMGLSAGGAMAMMLGLRYPDRFIGVSSHAGAVPYSAGNTAQAGAA
MRGSRVPDAAARQALRLWLAGRPLPPLLLLHGDRDSVVSVENAMTAARLWLDLLPDPSVRLVVPGARRVQRGQRLPAEVS
DWLVDGEIFVRLVRIEGLGHAWSGGPAAQAFSDPRGPDALRIALRFFGDR
>Mature_369_residues
GRRGGSTARLWTALGKSATRQARRMGREMTRAINKSVTKPMTEAIVRNAVKQSSAVTKATQRALSGVASPVPPPQSRGSG
RWEEGVGGLGPLAQRNYRIFIPAGVTARRRAPMLVLLHGCGQDAASFATVTRAVTVAREAGCVVLLPEQSSQGNAQRCWN
WFRPATRGSAEAALLMAMIDHVCGRYPVRPDRVSVMGLSAGGAMAMMLGLRYPDRFIGVSSHAGAVPYSAGNTAQAGAAM
RGSRVPDAAARQALRLWLAGRPLPPLLLLHGDRDSVVSVENAMTAARLWLDLLPDPSVRLVVPGARRVQRGQRLPAEVSD
WLVDGEIFVRLVRIEGLGHAWSGGPAAQAFSDPRGPDALRIALRFFGDR

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 39587; Mature: 39456

Theoretical pI: Translated: 12.03; Mature: 12.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRRGGSTARLWTALGKSATRQARRMGREMTRAINKSVTKPMTEAIVRNAVKQSSAVTKA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQRALSGVASPVPPPQSRGSGRWEEGVGGLGPLAQRNYRIFIPAGVTARRRAPMLVLLHG
HHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCHHHCCEEEEEECCCCCCCCCCEEEEEEC
CGQDAASFATVTRAVTVAREAGCVVLLPEQSSQGNAQRCWNWFRPATRGSAEAALLMAMI
CCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHH
DHVCGRYPVRPDRVSVMGLSAGGAMAMMLGLRYPDRFIGVSSHAGAVPYSAGNTAQAGAA
HHHHCCCCCCCCCEEEEEECCCCHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCHHCCHH
MRGSRVPDAAARQALRLWLAGRPLPPLLLLHGDRDSVVSVENAMTAARLWLDLLPDPSVR
HCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCCEE
LVVPGARRVQRGQRLPAEVSDWLVDGEIFVRLVRIEGLGHAWSGGPAAQAFSDPRGPDAL
EECCCHHHHHHCCCCCHHHHHHEECHHHHEEEEEECCCCCCCCCCCHHHHCCCCCCCHHH
RIALRFFGDR
HHHHHHHCCC
>Mature Secondary Structure 
GRRGGSTARLWTALGKSATRQARRMGREMTRAINKSVTKPMTEAIVRNAVKQSSAVTKA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQRALSGVASPVPPPQSRGSGRWEEGVGGLGPLAQRNYRIFIPAGVTARRRAPMLVLLHG
HHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCHHHCCEEEEEECCCCCCCCCCEEEEEEC
CGQDAASFATVTRAVTVAREAGCVVLLPEQSSQGNAQRCWNWFRPATRGSAEAALLMAMI
CCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHH
DHVCGRYPVRPDRVSVMGLSAGGAMAMMLGLRYPDRFIGVSSHAGAVPYSAGNTAQAGAA
HHHHCCCCCCCCCEEEEEECCCCHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCHHCCHH
MRGSRVPDAAARQALRLWLAGRPLPPLLLLHGDRDSVVSVENAMTAARLWLDLLPDPSVR
HCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCCEE
LVVPGARRVQRGQRLPAEVSDWLVDGEIFVRLVRIEGLGHAWSGGPAAQAFSDPRGPDAL
EECCCHHHHHHCCCCCHHHHHHEECHHHHEEEEEECCCCCCCCCCCHHHHCCCCCCCHHH
RIALRFFGDR
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]