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The map label for this gene is pcaU [H]

Identifier: 73538859

GI number: 73538859

Start: 1768167

End: 1768934

Strand: Reverse

Name: pcaU [H]

Synonym: Reut_B5034

Alternate gene names: 73538859

Gene position: 1768934-1768167 (Counterclockwise)

Preceding gene: 73538862

Following gene: 73538857

Centisome position: 64.89

GC content: 66.67

Gene sequence:

>768_bases
ATGGAAAAAGAACCGCTGAACCGGCGTGACTGGATCGCAGGGCTGGAAAAGGGCCTGGCCATCCTGGAGGCCTTCGACAA
CGACCATCCGCGGCTGACGCCAAGCCAGGCGGCGCAGTTGACGGGCCTGACCCGCACCGCTGCGCGGCGCTACCTGCTCA
CACTCGAACACCTTGGCTACACCACCAGCGACGGTACGCTGTTCAGCCTGACGCCGCGGGTGCTCAAGGTCGGCTGGTCC
TACTTCGATTCGGCGCGACTGCCACGCTTGGTGCAGCCGTTCCTGCAGCAGATCACGGCCCAGTTGGGTGAGGCGGCTTA
CCTGAGCGTGCTAGATGGCTGGGAGCTGGTGTTCATCTCCCGCACCAGCACCAGCCGCGTGATGAGCACGGGCTTCGTGC
TGGGCGCGCGCGTTCCGGCGCCACTGGCCTCGGCGGGCGTGATGATGCTGGCGTCGCAGCCGGAGGACACGGTGCGGGAG
TGGCTGGACCAGTGCGTGCTGACGCCCTATACGCCATACACCATCCTCCAGCACGACCGATTGCTGCAGGAAATCCGCAA
GGCCGGCGCGCAGGGCTATGCCGTGGTCGAGCAGCAGCTGCAGATTGGCGTGCGTGGCGTCGCGGTGCCGCTGCGCGATC
GCCACGGCAAGGTGATCGCCGCGCTGTCGGTCAATATGCAGATCGGCGAGGAGAGCGCGGAAGCGGCATGCGCGCGCGTG
CTGCAGGTGCTGCAGGACACCGCGCTGTCGATCATGCGTACGCTGTAG

Upstream 100 bases:

>100_bases
AGGTTAGGAGCCAACTGTGGGCCAAACAATCGCAATTCGCGCGCCGACGAGCGATAATCGCGCGATGGCGCGCGACAGTC
GTGCATTACTCGGGTTAACC

Downstream 100 bases:

>100_bases
CGCCCATGCGCCTGCCGTGCTAAGCGCAGTGGCTTACTTGTGCAATGAACTCGCCAGCGCGAACGGCCCCTGCGACAGGC
GGTCCTTGAGAAATTCGATG

Product: regulatory protein IclR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MEKEPLNRRDWIAGLEKGLAILEAFDNDHPRLTPSQAAQLTGLTRTAARRYLLTLEHLGYTTSDGTLFSLTPRVLKVGWS
YFDSARLPRLVQPFLQQITAQLGEAAYLSVLDGWELVFISRTSTSRVMSTGFVLGARVPAPLASAGVMMLASQPEDTVRE
WLDQCVLTPYTPYTILQHDRLLQEIRKAGAQGYAVVEQQLQIGVRGVAVPLRDRHGKVIAALSVNMQIGEESAEAACARV
LQVLQDTALSIMRTL

Sequences:

>Translated_255_residues
MEKEPLNRRDWIAGLEKGLAILEAFDNDHPRLTPSQAAQLTGLTRTAARRYLLTLEHLGYTTSDGTLFSLTPRVLKVGWS
YFDSARLPRLVQPFLQQITAQLGEAAYLSVLDGWELVFISRTSTSRVMSTGFVLGARVPAPLASAGVMMLASQPEDTVRE
WLDQCVLTPYTPYTILQHDRLLQEIRKAGAQGYAVVEQQLQIGVRGVAVPLRDRHGKVIAALSVNMQIGEESAEAACARV
LQVLQDTALSIMRTL
>Mature_255_residues
MEKEPLNRRDWIAGLEKGLAILEAFDNDHPRLTPSQAAQLTGLTRTAARRYLLTLEHLGYTTSDGTLFSLTPRVLKVGWS
YFDSARLPRLVQPFLQQITAQLGEAAYLSVLDGWELVFISRTSTSRVMSTGFVLGARVPAPLASAGVMMLASQPEDTVRE
WLDQCVLTPYTPYTILQHDRLLQEIRKAGAQGYAVVEQQLQIGVRGVAVPLRDRHGKVIAALSVNMQIGEESAEAACARV
LQVLQDTALSIMRTL

Specific function: Activates transcription of the pca operon [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=246, Percent_Identity=25.609756097561, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI87082359, Length=218, Percent_Identity=25.2293577981651, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788131, Length=228, Percent_Identity=25, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1789999, Length=269, Percent_Identity=24.907063197026, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786715, Length=226, Percent_Identity=24.3362831858407, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI145693096, Length=225, Percent_Identity=29.3333333333333, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 28159; Mature: 28159

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKEPLNRRDWIAGLEKGLAILEAFDNDHPRLTPSQAAQLTGLTRTAARRYLLTLEHLGY
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCE
TTSDGTLFSLTPRVLKVGWSYFDSARLPRLVQPFLQQITAQLGEAAYLSVLDGWELVFIS
ECCCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEE
RTSTSRVMSTGFVLGARVPAPLASAGVMMLASQPEDTVREWLDQCVLTPYTPYTILQHDR
CCCHHHHHHHHHHEECCCCCCHHHCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHH
LLQEIRKAGAQGYAVVEQQLQIGVRGVAVPLRDRHGKVIAALSVNMQIGEESAEAACARV
HHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEEEEECCHHHHHHHHHH
LQVLQDTALSIMRTL
HHHHHHHHHHHHHHC
>Mature Secondary Structure
MEKEPLNRRDWIAGLEKGLAILEAFDNDHPRLTPSQAAQLTGLTRTAARRYLLTLEHLGY
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCE
TTSDGTLFSLTPRVLKVGWSYFDSARLPRLVQPFLQQITAQLGEAAYLSVLDGWELVFIS
ECCCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEE
RTSTSRVMSTGFVLGARVPAPLASAGVMMLASQPEDTVREWLDQCVLTPYTPYTILQHDR
CCCHHHHHHHHHHEECCCCCCHHHCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHH
LLQEIRKAGAQGYAVVEQQLQIGVRGVAVPLRDRHGKVIAALSVNMQIGEESAEAACARV
HHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEEEEECCHHHHHHHHHH
LQVLQDTALSIMRTL
HHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515921 [H]