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The map label for this gene is gatA [H]

Identifier: 73538707

GI number: 73538707

Start: 1600949

End: 1602778

Strand: Reverse

Name: gatA [H]

Synonym: Reut_B4882

Alternate gene names: 73538707

Gene position: 1602778-1600949 (Counterclockwise)

Preceding gene: 73538708

Following gene: 73538704

Centisome position: 58.79

GC content: 68.58

Gene sequence:

>1830_bases
ATGAACACGCCCGCCCGCGCCGTGCCGTTTTCACTGGATATTGCCTCGCTGAAGGATGACTACCGCAGCGGCAGGCGTAC
GCCGCTCGATGTTGTGGCAGCGGTGATTGCCGCCACGGCCGACGATCCGCACCATGCCTGGATCCATCGGGTCGATGCAG
GCCGATTGCGCGAACGCGCCCGCGCACTGATGGCGCACGATCCTGCCACGCTGCCGCTCTATGGCATTCCGTTCGCGATC
AAGGACAACATCGACCTGGCCGGCTGCCCGACCACTGCGGCGTGCCCCGAGTACGCCTATGTCCCGCAACAGAGTGCCTT
CGTCGTGCAGCGGCTTATCGATGCCGGCGCGATTGCCATCGGCAAGACGAACCTCGACCAGTTCGCGACGGGGCTGAACG
GCACGCGCTCGCCTTATGGTGCGTGCCGCAATGCGTTCGATCCGGACTACGTATCGGGAGGCTCCAGTTCGGGGTCGGCG
GTGGCAGTGGCGCTGGGCCACGTGAGCTTCGCGCTCGGCACGGACACCGCGGGCTCGGGCCGCGTGCCGGCCGCGTTCAA
TAACCTCGTGGGACTCAAGCCCACCTGCGGGCTGCTGTCCGCGACGGGTATCGTGCCGGCGTGCCGCTCGCTCGATACCG
CGTCAATCTTTGCCTTAACCGCGGACGATGCGCACCGCGTCTTTGCCGTGGCACACGGGTTCGACGAAGCCGACGCGTTC
TCGCGAAAGGCCTCGCCACATGGCTTCGATTTCGGCAGTGCGCCGTCCTTCCGCGTGGGCGTGCCGGCGCTTCATGCGTT
GGGCAACCTGGCGCCTGATTATGCCGAGGCCTGGGGGCGGGCGCTTGATCATGCACGCAGCCTTGGCGCGGAGCTGGTGG
AGATCGACATCGCGCCGTTCCTGGAGACCGCACGCCTGCTCTACGAAGGGCCATGGGTGGCCGAGCGTTACCAGGCCATC
CGCGCATTCATTGACGCGAACCCGAAGGCCGTGTTCCCGGTCACGGGGCAGATCACGCTCGGCGGCGCCGCACCGTTGGC
CGCCGATGCCTTTGCCGCGACATATCGACTGCGCGACCTGCGGCGCCGGTGCGATGCGGTATGGGTGCAGGTGGATGCCA
TGCTGTTGCCGACGGTGCCGCAGCATCCCACCATCGCGGCCATGCTGGCGGACCCGATCGGGCTGAACAGTGAACTTGGC
CGCTTTACCAATTTCATGAACCTGCTCGACTACGGCGGCATTGCGGTGCCTGCAGGCTTGACCGCGCTCGGCCTGCCGTT
TGGCGTGACCGTAGTGGCGCCTGCGCAGCAGGATGTACCGCTGCTGCGGCTGGCACGCCGCTGGCAGCAGGCGCTGCAGG
GGCGAGCTGCGACGATGGGGGCGACGGGCAGGCCATTGCCGCAAGGTGGCGCAGTTCCCCGTGCCGTGCCGTCTGGCCAG
ATCCGCGTGGCCGTGTGCGGCGCGCACCTGCAAGGCCAGCCTTTGCACCACCAACTCGCCGACCGCGGCGCCCGACTTGT
CGCGCGCACGCGCACCGCGCCGTGCTACCGGTTCTATGCGTTGAGCGGCGAAGGCCCGCGCCGGCCGGGGCTGGTCCGCG
TGGCCGAGGGCGGCGCGGCGATCGAGGTGGAAGTCTGGGAGTTGCCGCAGACGCAGTTCGGCAGCTTCGTCGCGGGCATT
CCCGCGCCGCTGGGCATTGGCCGCACACTGCTGGCAGATGGTACCGAGGTGCCAGGCTTTATCTGCGAGCCGGCAGGTGT
GTCGGGCGCGACCGACATCACCCGCTGGGGCGGATGGCGCGCGTGGCTGGCGCGGGCCGAGGCGGCCTGA

Upstream 100 bases:

>100_bases
GCTGCGCCGAAGGGGCGCCGGTCGGCGCGGGACAGGAGATGGCTGTGGTCGTACCGGACGTCGTGCAGGTTTGCGCCGAT
ATGGCAAGCGAGGTGGCAGC

Downstream 100 bases:

>100_bases
GCTGCCTTCAGGTGCGCACCGATGCGCGCCACGCGCCGGGCGGTTGGCCGAACCGGCGTGTGAAACTGCGCGTGAAGGTG
GTCTGGTCGGGATAGCCGGA

Product: allophanate hydrolase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 609; Mature: 609

Protein sequence:

>609_residues
MNTPARAVPFSLDIASLKDDYRSGRRTPLDVVAAVIAATADDPHHAWIHRVDAGRLRERARALMAHDPATLPLYGIPFAI
KDNIDLAGCPTTAACPEYAYVPQQSAFVVQRLIDAGAIAIGKTNLDQFATGLNGTRSPYGACRNAFDPDYVSGGSSSGSA
VAVALGHVSFALGTDTAGSGRVPAAFNNLVGLKPTCGLLSATGIVPACRSLDTASIFALTADDAHRVFAVAHGFDEADAF
SRKASPHGFDFGSAPSFRVGVPALHALGNLAPDYAEAWGRALDHARSLGAELVEIDIAPFLETARLLYEGPWVAERYQAI
RAFIDANPKAVFPVTGQITLGGAAPLAADAFAATYRLRDLRRRCDAVWVQVDAMLLPTVPQHPTIAAMLADPIGLNSELG
RFTNFMNLLDYGGIAVPAGLTALGLPFGVTVVAPAQQDVPLLRLARRWQQALQGRAATMGATGRPLPQGGAVPRAVPSGQ
IRVAVCGAHLQGQPLHHQLADRGARLVARTRTAPCYRFYALSGEGPRRPGLVRVAEGGAAIEVEVWELPQTQFGSFVAGI
PAPLGIGRTLLADGTEVPGFICEPAGVSGATDITRWGGWRAWLARAEAA

Sequences:

>Translated_609_residues
MNTPARAVPFSLDIASLKDDYRSGRRTPLDVVAAVIAATADDPHHAWIHRVDAGRLRERARALMAHDPATLPLYGIPFAI
KDNIDLAGCPTTAACPEYAYVPQQSAFVVQRLIDAGAIAIGKTNLDQFATGLNGTRSPYGACRNAFDPDYVSGGSSSGSA
VAVALGHVSFALGTDTAGSGRVPAAFNNLVGLKPTCGLLSATGIVPACRSLDTASIFALTADDAHRVFAVAHGFDEADAF
SRKASPHGFDFGSAPSFRVGVPALHALGNLAPDYAEAWGRALDHARSLGAELVEIDIAPFLETARLLYEGPWVAERYQAI
RAFIDANPKAVFPVTGQITLGGAAPLAADAFAATYRLRDLRRRCDAVWVQVDAMLLPTVPQHPTIAAMLADPIGLNSELG
RFTNFMNLLDYGGIAVPAGLTALGLPFGVTVVAPAQQDVPLLRLARRWQQALQGRAATMGATGRPLPQGGAVPRAVPSGQ
IRVAVCGAHLQGQPLHHQLADRGARLVARTRTAPCYRFYALSGEGPRRPGLVRVAEGGAAIEVEVWELPQTQFGSFVAGI
PAPLGIGRTLLADGTEVPGFICEPAGVSGATDITRWGGWRAWLARAEAA
>Mature_609_residues
MNTPARAVPFSLDIASLKDDYRSGRRTPLDVVAAVIAATADDPHHAWIHRVDAGRLRERARALMAHDPATLPLYGIPFAI
KDNIDLAGCPTTAACPEYAYVPQQSAFVVQRLIDAGAIAIGKTNLDQFATGLNGTRSPYGACRNAFDPDYVSGGSSSGSA
VAVALGHVSFALGTDTAGSGRVPAAFNNLVGLKPTCGLLSATGIVPACRSLDTASIFALTADDAHRVFAVAHGFDEADAF
SRKASPHGFDFGSAPSFRVGVPALHALGNLAPDYAEAWGRALDHARSLGAELVEIDIAPFLETARLLYEGPWVAERYQAI
RAFIDANPKAVFPVTGQITLGGAAPLAADAFAATYRLRDLRRRCDAVWVQVDAMLLPTVPQHPTIAAMLADPIGLNSELG
RFTNFMNLLDYGGIAVPAGLTALGLPFGVTVVAPAQQDVPLLRLARRWQQALQGRAATMGATGRPLPQGGAVPRAVPSGQ
IRVAVCGAHLQGQPLHHQLADRGARLVARTRTAPCYRFYALSGEGPRRPGLVRVAEGGAAIEVEVWELPQTQFGSFVAGI
PAPLGIGRTLLADGTEVPGFICEPAGVSGATDITRWGGWRAWLARAEAA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=433, Percent_Identity=27.9445727482679, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI195972892, Length=197, Percent_Identity=29.4416243654822, Blast_Score=77, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=395, Percent_Identity=29.620253164557, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17556264, Length=159, Percent_Identity=35.2201257861635, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17538252, Length=205, Percent_Identity=27.8048780487805, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17537465, Length=136, Percent_Identity=33.0882352941176, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=243, Percent_Identity=25.9259259259259, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=577, Percent_Identity=43.6741767764298, Blast_Score=420, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6323950, Length=311, Percent_Identity=30.2250803858521, Blast_Score=90, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24648113, Length=428, Percent_Identity=30.1401869158879, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24652985, Length=422, Percent_Identity=26.7772511848341, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922090, Length=422, Percent_Identity=26.7772511848341, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24652981, Length=422, Percent_Identity=26.7772511848341, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24652983, Length=422, Percent_Identity=26.7772511848341, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24644968, Length=209, Percent_Identity=28.2296650717703, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 64035; Mature: 64035

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPARAVPFSLDIASLKDDYRSGRRTPLDVVAAVIAATADDPHHAWIHRVDAGRLRERA
CCCCCCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHEEECCHHHHHHHH
RALMAHDPATLPLYGIPFAIKDNIDLAGCPTTAACPEYAYVPQQSAFVVQRLIDAGAIAI
HHHHHCCCCCCEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEE
GKTNLDQFATGLNGTRSPYGACRNAFDPDYVSGGSSSGSAVAVALGHVSFALGTDTAGSG
CCCCHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEEEHEEEEECCCCCCCC
RVPAAFNNLVGLKPTCGLLSATGIVPACRSLDTASIFALTADDAHRVFAVAHGFDEADAF
CCCHHHHCCCCCCCCCCHHHHCCHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCHHHHH
SRKASPHGFDFGSAPSFRVGVPALHALGNLAPDYAEAWGRALDHARSLGAELVEIDIAPF
HCCCCCCCCCCCCCCCCEECCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHH
LETARLLYEGPWVAERYQAIRAFIDANPKAVFPVTGQITLGGAAPLAADAFAATYRLRDL
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECEEEECCCCCHHHHHHHHHHHHHHH
RRRCDAVWVQVDAMLLPTVPQHPTIAAMLADPIGLNSELGRFTNFMNLLDYGGIAVPAGL
HHHHHEEEEEEEEEEECCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCEECCCCH
TALGLPFGVTVVAPAQQDVPLLRLARRWQQALQGRAATMGATGRPLPQGGAVPRAVPSGQ
HHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCC
IRVAVCGAHLQGQPLHHQLADRGARLVARTRTAPCYRFYALSGEGPRRPGLVRVAEGGAA
EEEEEECCCCCCCCHHHHHHHCCCEEEHHCCCCCEEEEEEECCCCCCCCCEEEEECCCCE
IEVEVWELPQTQFGSFVAGIPAPLGIGRTLLADGTEVPGFICEPAGVSGATDITRWGGWR
EEEEEEECCCHHHCCCHHCCCCCCCCCCCEECCCCCCCCEEECCCCCCCCCCHHHCCCHH
AWLARAEAA
HHHHHHCCC
>Mature Secondary Structure
MNTPARAVPFSLDIASLKDDYRSGRRTPLDVVAAVIAATADDPHHAWIHRVDAGRLRERA
CCCCCCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHEEECCHHHHHHHH
RALMAHDPATLPLYGIPFAIKDNIDLAGCPTTAACPEYAYVPQQSAFVVQRLIDAGAIAI
HHHHHCCCCCCEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEE
GKTNLDQFATGLNGTRSPYGACRNAFDPDYVSGGSSSGSAVAVALGHVSFALGTDTAGSG
CCCCHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEEEHEEEEECCCCCCCC
RVPAAFNNLVGLKPTCGLLSATGIVPACRSLDTASIFALTADDAHRVFAVAHGFDEADAF
CCCHHHHCCCCCCCCCCHHHHCCHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCHHHHH
SRKASPHGFDFGSAPSFRVGVPALHALGNLAPDYAEAWGRALDHARSLGAELVEIDIAPF
HCCCCCCCCCCCCCCCCEECCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHH
LETARLLYEGPWVAERYQAIRAFIDANPKAVFPVTGQITLGGAAPLAADAFAATYRLRDL
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECEEEECCCCCHHHHHHHHHHHHHHH
RRRCDAVWVQVDAMLLPTVPQHPTIAAMLADPIGLNSELGRFTNFMNLLDYGGIAVPAGL
HHHHHEEEEEEEEEEECCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCEECCCCH
TALGLPFGVTVVAPAQQDVPLLRLARRWQQALQGRAATMGATGRPLPQGGAVPRAVPSGQ
HHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCC
IRVAVCGAHLQGQPLHHQLADRGARLVARTRTAPCYRFYALSGEGPRRPGLVRVAEGGAA
EEEEEECCCCCCCCHHHHHHHCCCEEEHHCCCCCEEEEEEECCCCCCCCCEEEEECCCCE
IEVEVWELPQTQFGSFVAGIPAPLGIGRTLLADGTEVPGFICEPAGVSGATDITRWGGWR
EEEEEEECCCHHHCCCHHCCCCCCCCCCCEECCCCCCCCEEECCCCCCCCCCHHHCCCHH
AWLARAEAA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]