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The map label for this gene is stcD [H]

Identifier: 73538644

GI number: 73538644

Start: 1520803

End: 1522866

Strand: Reverse

Name: stcD [H]

Synonym: Reut_B4819

Alternate gene names: 73538644

Gene position: 1522866-1520803 (Counterclockwise)

Preceding gene: 73538645

Following gene: 73538643

Centisome position: 55.86

GC content: 65.12

Gene sequence:

>2064_bases
ATGCGTTATCCCCACTTGTTCAAACCTTTGCAGCTCAATCAGCTGACGCTCCGCAACCGGATCGTGAGCACCGCGCACGC
GGAAGTGTATGCCGAGCCGGGCGGCCTGCCAGGTGACCGCTACATTCGCTACTACGAAGAGAAGGCAAAGGGCGGCGTCG
GCCTGGCAATTTGCGGCGGCTCGAGCCCCGTGTCGATCGACAGTCCCCAGGGCTGGTGGAAGTCGGTGAACCTCTCGACC
GACAAGATCATCGATCCGCTGTCGCGGCTGGCCGAGACGATGCACCGGCATGGCGCCAAGATCATGATCCAGGCCACGCA
CATGGGACGCCGCTCGGCGTTCCATGGCGAGCACTGGCCACACCTGATGACGCCATCCGGCGTGCGCGAGCCCGTGCACC
GTGGCAACGCCAAGATCATCGAGGTCGAGGAGATCCGCCGCATCATCGCCGACTTCGCGGCAGCCGCGAAGCGCGTGAAG
GACGCGGGCATGGATGGCATCGAGATCTCGGCCGCCCACCAGCACCTGATCGACCAGTTCTGGAGCCCGCGCACGAACTT
CCGCGCCGATGAGTGGGGCGGTAGCCTCGAGAACCGCATGCGCTTCGGCGTGGAGGTGCTCCAGGCGGTGCGTGAGGTGG
TCGGCAAGGATTTCTGCGTGGGGCTGCGCATGTGCGGCGACGAGTTCCATGAAGACGGTCTCGATCACGAGCAACTGAAG
GAGATCGCGCAGGCGATGTCCGAGTCCGGGCTCGTCGACTATATCGGCGTGGTCGGCTCCGGCGCCGATACGCACAACAC
GCTCGCAAACTGCATGCCGCCGATGGCACTGCCGCCTGAGCCGTTCGTGCATCTGGCCGCCGGTATCAAGTCGGTGATCA
AGGTACCGTTGATGCACGCGCAGAGCATCCGCGACGCGGGCCAGGCCGAGCGCCTGCTCGCGAACGGCATGGTCGACCTC
GTCGGCATGACGCGCGCCCAGATCGCCGACCCGCACATGGTCATCAAGATCCGCGACGGCCGCGAAGACGAGATCAAGCA
ATGCGTCGGCGCGAACTATTGCATTGACCGTCAGTACAACGGCCTCGACGTACTGTGCGTGCAAAACGCCGCGACCTCGC
GCGAGGCGACGATGCCGCACATCATCGAAAAGTCGCGTGGCCCGAAGCGCAAGGTGGTGGTTGTTGGTGCGGGACCGGCC
GGCCTGGAAGCGGCACGCGTCGCGCGCTCACGCGGCCACGACGTCGTGCTGTTCGAGAAGAACACGGAAGTCGGCGGACA
GATCCTGCTCGCTGCCAAGGCACCGCAGCGCGAGCAGATGCAGGGCATCGTGCGCTGGTTCGACATGGAGACCAAGCGCC
TTGGCGTCGACCGGCGCCTTGGCGTTGAAGCGGATGCAAAGATGATCATGGCGGAGAAGCCGGACATCATCGTGCTCGCT
ACGGGCGGCCGGGCATTCTCCGATCAGGTGCCGGGCTGGGCCGTGGCCGAGGGGCTCGCGGTCAGTTCCTGGGACGTGCT
GGCCGGCCGCGTCGAGGCGAAGGAGAACGTGCTCGTCTATGACGGCGTGAGCACGCACGCCGGTGCAGGCGTGGCCGACT
ACCTGGCGAGCCGCGGCGCGAAGGTCGAGATCGTGACGCCGGACGTCAAGGTTGCCGATGATTGCGGCGGCACCACCTTC
CCGATCTTCTATCGCCGCCTGTACGCGCAGGGCGTGATCCATACGCCGAACTACTGGCTCGATCGTGTCTACGAGGAGGG
CGGCAAGAAGATCGCGGTGCTGCGCAACGAGTACACGGAAGAACTCGAGGAACGCGCGGTCGACCAGGTCGTGATCGAGA
ACGGCTCGACCCCGAACGACGCGCTGTACTGGAAGCTCAAGCCGGAATCGCTCAATCGCGGCCAGGTCGACGTGCACAAG
CTGTTTGCGTCGGAGCCGCAGCCGAGCCTGTCGGAAACGCTCGGCAACGGCCGCTTCCTGTTGTTCCGCGTCGGCGACTG
CATCTCGATGCACAACATCCACGGCGCGATCTACGACGCGCTGCGGCTCGTGAAGGATTTCTGA

Upstream 100 bases:

>100_bases
GATCACTGCGTGTTCGAAGTGTCGCCGATCGCGCGCTGACACCGAAGCCCGTCCGCACTACGAGAAATACGAGACACACC
AACGCCAGAGGTCGTCCGCG

Downstream 100 bases:

>100_bases
CGATGAACCCGTCCCTCCTGATTACCGTTCTGCTGTGGCTGTCGGTGGCGGGGCTCGCATTCGCGGTCGTGAAGCGTGCG
TCGTACTGGCGGCTCGGCCG

Product: NADH:-lavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: Stachydrine utilization protein stcD [H]

Number of amino acids: Translated: 687; Mature: 687

Protein sequence:

>687_residues
MRYPHLFKPLQLNQLTLRNRIVSTAHAEVYAEPGGLPGDRYIRYYEEKAKGGVGLAICGGSSPVSIDSPQGWWKSVNLST
DKIIDPLSRLAETMHRHGAKIMIQATHMGRRSAFHGEHWPHLMTPSGVREPVHRGNAKIIEVEEIRRIIADFAAAAKRVK
DAGMDGIEISAAHQHLIDQFWSPRTNFRADEWGGSLENRMRFGVEVLQAVREVVGKDFCVGLRMCGDEFHEDGLDHEQLK
EIAQAMSESGLVDYIGVVGSGADTHNTLANCMPPMALPPEPFVHLAAGIKSVIKVPLMHAQSIRDAGQAERLLANGMVDL
VGMTRAQIADPHMVIKIRDGREDEIKQCVGANYCIDRQYNGLDVLCVQNAATSREATMPHIIEKSRGPKRKVVVVGAGPA
GLEAARVARSRGHDVVLFEKNTEVGGQILLAAKAPQREQMQGIVRWFDMETKRLGVDRRLGVEADAKMIMAEKPDIIVLA
TGGRAFSDQVPGWAVAEGLAVSSWDVLAGRVEAKENVLVYDGVSTHAGAGVADYLASRGAKVEIVTPDVKVADDCGGTTF
PIFYRRLYAQGVIHTPNYWLDRVYEEGGKKIAVLRNEYTEELEERAVDQVVIENGSTPNDALYWKLKPESLNRGQVDVHK
LFASEPQPSLSETLGNGRFLLFRVGDCISMHNIHGAIYDALRLVKDF

Sequences:

>Translated_687_residues
MRYPHLFKPLQLNQLTLRNRIVSTAHAEVYAEPGGLPGDRYIRYYEEKAKGGVGLAICGGSSPVSIDSPQGWWKSVNLST
DKIIDPLSRLAETMHRHGAKIMIQATHMGRRSAFHGEHWPHLMTPSGVREPVHRGNAKIIEVEEIRRIIADFAAAAKRVK
DAGMDGIEISAAHQHLIDQFWSPRTNFRADEWGGSLENRMRFGVEVLQAVREVVGKDFCVGLRMCGDEFHEDGLDHEQLK
EIAQAMSESGLVDYIGVVGSGADTHNTLANCMPPMALPPEPFVHLAAGIKSVIKVPLMHAQSIRDAGQAERLLANGMVDL
VGMTRAQIADPHMVIKIRDGREDEIKQCVGANYCIDRQYNGLDVLCVQNAATSREATMPHIIEKSRGPKRKVVVVGAGPA
GLEAARVARSRGHDVVLFEKNTEVGGQILLAAKAPQREQMQGIVRWFDMETKRLGVDRRLGVEADAKMIMAEKPDIIVLA
TGGRAFSDQVPGWAVAEGLAVSSWDVLAGRVEAKENVLVYDGVSTHAGAGVADYLASRGAKVEIVTPDVKVADDCGGTTF
PIFYRRLYAQGVIHTPNYWLDRVYEEGGKKIAVLRNEYTEELEERAVDQVVIENGSTPNDALYWKLKPESLNRGQVDVHK
LFASEPQPSLSETLGNGRFLLFRVGDCISMHNIHGAIYDALRLVKDF
>Mature_687_residues
MRYPHLFKPLQLNQLTLRNRIVSTAHAEVYAEPGGLPGDRYIRYYEEKAKGGVGLAICGGSSPVSIDSPQGWWKSVNLST
DKIIDPLSRLAETMHRHGAKIMIQATHMGRRSAFHGEHWPHLMTPSGVREPVHRGNAKIIEVEEIRRIIADFAAAAKRVK
DAGMDGIEISAAHQHLIDQFWSPRTNFRADEWGGSLENRMRFGVEVLQAVREVVGKDFCVGLRMCGDEFHEDGLDHEQLK
EIAQAMSESGLVDYIGVVGSGADTHNTLANCMPPMALPPEPFVHLAAGIKSVIKVPLMHAQSIRDAGQAERLLANGMVDL
VGMTRAQIADPHMVIKIRDGREDEIKQCVGANYCIDRQYNGLDVLCVQNAATSREATMPHIIEKSRGPKRKVVVVGAGPA
GLEAARVARSRGHDVVLFEKNTEVGGQILLAAKAPQREQMQGIVRWFDMETKRLGVDRRLGVEADAKMIMAEKPDIIVLA
TGGRAFSDQVPGWAVAEGLAVSSWDVLAGRVEAKENVLVYDGVSTHAGAGVADYLASRGAKVEIVTPDVKVADDCGGTTF
PIFYRRLYAQGVIHTPNYWLDRVYEEGGKKIAVLRNEYTEELEERAVDQVVIENGSTPNDALYWKLKPESLNRGQVDVHK
LFASEPQPSLSETLGNGRFLLFRVGDCISMHNIHGAIYDALRLVKDF

Specific function: Possible NADH-dependent oxidase, functions as a demethylase that converts N-methylproline to proline [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789463, Length=666, Percent_Identity=31.2312312312312, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1787939, Length=359, Percent_Identity=25.6267409470752, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17566914, Length=256, Percent_Identity=32.421875, Blast_Score=112, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17559804, Length=301, Percent_Identity=30.8970099667774, Blast_Score=105, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17540738, Length=344, Percent_Identity=23.8372093023256, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17559802, Length=346, Percent_Identity=27.1676300578035, Blast_Score=103, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17564188, Length=372, Percent_Identity=27.1505376344086, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17565138, Length=336, Percent_Identity=24.702380952381, Blast_Score=96, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=346, Percent_Identity=26.0115606936416, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321973, Length=242, Percent_Identity=29.3388429752066, Blast_Score=94, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6325086, Length=246, Percent_Identity=27.6422764227642, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 75699; Mature: 75699

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYPHLFKPLQLNQLTLRNRIVSTAHAEVYAEPGGLPGDRYIRYYEEKAKGGVGLAICGG
CCCCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECC
SSPVSIDSPQGWWKSVNLSTDKIIDPLSRLAETMHRHGAKIMIQATHMGRRSAFHGEHWP
CCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCC
HLMTPSGVREPVHRGNAKIIEVEEIRRIIADFAAAAKRVKDAGMDGIEISAAHQHLIDQF
CCCCCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
WSPRTNFRADEWGGSLENRMRFGVEVLQAVREVVGKDFCVGLRMCGDEFHEDGLDHEQLK
CCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHH
EIAQAMSESGLVDYIGVVGSGADTHNTLANCMPPMALPPEPFVHLAAGIKSVIKVPLMHA
HHHHHHHHCCCEEEHHHHCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QSIRDAGQAERLLANGMVDLVGMTRAQIADPHMVIKIRDGREDEIKQCVGANYCIDRQYN
HHHHHHHHHHHHHHCCHHHHHHCHHHHCCCCEEEEEECCCCHHHHHHHHCCCCEECCCCC
GLDVLCVQNAATSREATMPHIIEKSRGPKRKVVVVGAGPAGLEAARVARSRGHDVVLFEK
CEEEEEECCCCCCCCCCCHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEC
NTEVGGQILLAAKAPQREQMQGIVRWFDMETKRLGVDRRLGVEADAKMIMAEKPDIIVLA
CCCCCCEEEEEECCCCHHHHHHHHHHHHCHHHHCCCCHHCCCCCCCEEEEECCCCEEEEE
TGGRAFSDQVPGWAVAEGLAVSSWDVLAGRVEAKENVLVYDGVSTHAGAGVADYLASRGA
CCCCCCCCCCCCHHHHCCCEECCHHHHHCCEECCCCEEEECCCCCCCCCCHHHHHHCCCC
KVEIVTPDVKVADDCGGTTFPIFYRRLYAQGVIHTPNYWLDRVYEEGGKKIAVLRNEYTE
EEEEECCCCEECCCCCCCHHHHHHHHHHHCCCEECCHHHHHHHHHHCCCEEEEEHHHHHH
ELEERAVDQVVIENGSTPNDALYWKLKPESLNRGQVDVHKLFASEPQPSLSETLGNGRFL
HHHHHHHHHHEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCHHHHCCCCCEE
LFRVGDCISMHNIHGAIYDALRLVKDF
EEEECCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRYPHLFKPLQLNQLTLRNRIVSTAHAEVYAEPGGLPGDRYIRYYEEKAKGGVGLAICGG
CCCCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECC
SSPVSIDSPQGWWKSVNLSTDKIIDPLSRLAETMHRHGAKIMIQATHMGRRSAFHGEHWP
CCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCC
HLMTPSGVREPVHRGNAKIIEVEEIRRIIADFAAAAKRVKDAGMDGIEISAAHQHLIDQF
CCCCCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
WSPRTNFRADEWGGSLENRMRFGVEVLQAVREVVGKDFCVGLRMCGDEFHEDGLDHEQLK
CCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHH
EIAQAMSESGLVDYIGVVGSGADTHNTLANCMPPMALPPEPFVHLAAGIKSVIKVPLMHA
HHHHHHHHCCCEEEHHHHCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QSIRDAGQAERLLANGMVDLVGMTRAQIADPHMVIKIRDGREDEIKQCVGANYCIDRQYN
HHHHHHHHHHHHHHCCHHHHHHCHHHHCCCCEEEEEECCCCHHHHHHHHCCCCEECCCCC
GLDVLCVQNAATSREATMPHIIEKSRGPKRKVVVVGAGPAGLEAARVARSRGHDVVLFEK
CEEEEEECCCCCCCCCCCHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEC
NTEVGGQILLAAKAPQREQMQGIVRWFDMETKRLGVDRRLGVEADAKMIMAEKPDIIVLA
CCCCCCEEEEEECCCCHHHHHHHHHHHHCHHHHCCCCHHCCCCCCCEEEEECCCCEEEEE
TGGRAFSDQVPGWAVAEGLAVSSWDVLAGRVEAKENVLVYDGVSTHAGAGVADYLASRGA
CCCCCCCCCCCCHHHHCCCEECCHHHHHCCEECCCCEEEECCCCCCCCCCHHHHHHCCCC
KVEIVTPDVKVADDCGGTTFPIFYRRLYAQGVIHTPNYWLDRVYEEGGKKIAVLRNEYTE
EEEEECCCCEECCCCCCCHHHHHHHHHHHCCCEECCHHHHHHHHHHCCCEEEEEHHHHHH
ELEERAVDQVVIENGSTPNDALYWKLKPESLNRGQVDVHKLFASEPQPSLSETLGNGRFL
HHHHHHHHHHEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCHHHHCCCCCEE
LFRVGDCISMHNIHGAIYDALRLVKDF
EEEECCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9758825; 11481431 [H]