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The map label for this gene is cysK [H]

Identifier: 73538568

GI number: 73538568

Start: 1442547

End: 1443464

Strand: Direct

Name: cysK [H]

Synonym: Reut_B4743

Alternate gene names: 73538568

Gene position: 1442547-1443464 (Clockwise)

Preceding gene: 73538567

Following gene: 73538570

Centisome position: 52.92

GC content: 65.36

Gene sequence:

>918_bases
ATGAAGGCAGACAACATCCTGCAGACCATCGGCAATACGCCGCATATCCGCATCCGACGGCTGTTCGGAAACGGCCACGA
AGTCTGGATCAAGTCGGAGCGGTCCAACCCGGGCGGCTCGATCAAGGACCGTATCGCGCTATCGATGGTGGAAGACGCCG
AACGCCGTGGCGTGCTCAAACCCGGTGGCACCATTGTCGAGCCGACCTCCGGCAATACCGGCATCGGCCTGGCGATGGTG
GCGGCAGTCAAGGGCTACAAGCTGATCCTGGTGATGCCGGACAGCATGTCGATCGAACGCCGCCGGCTGATGCTCGCCTA
CGGCGCGACCTTTGACCTGACCCCGCGCGAGAAAGGCATGAAGGGCGCGATCGCCCGCGCCGAGGAATTGATCGCCTCGA
TCCCCGGCGCGTGGATGCCGCAGCAATTCGAGAATCCGGCCAACATCGACGTCCATGCGCGCACCACGGCGCAGGAAATC
CTGGCGGACTTCCCGGAAGGCCTCGATGCACTGATTACCGGCGTGGGCACGGGCGGCCATATCTCGGGCTGCGCGCAGGT
GCTCAAGCAGAAATGGCCGCAACTGAAGGTGTTCGCCGTGGAGCCGACGGCATCGCCGGTGATTTCGGGTGGCGCGCCCG
CGCCGCATCCGATCCAGGGCATCGGCGCCGGTTTTATCCCGGAGAACCTGAAAACCGACCTGCTCGATGGCGTGATCCAG
GTCGATGCCGAACCAGCGCGCGAGATGGCGCGCCGCTGCGCGCGCGAAGAAGGCATCCTGGTTGGCATTTCGTCGGGCGC
GACGCTGGCCGCGATCGCGAGCAAGCTGCCTGAACTTCCGGCCGGCGCCCGCGTGCTCGGGTTCAACTATGACACCGGCG
AGCGTTACCTGACGGTCGAGGGCTTCCTGCCGGCCTGA

Upstream 100 bases:

>100_bases
TGACGCATGCGACCAAGGCTCTGTGAACTTTAATAGAATCCCGGTCTGACACGCATTCGCGCCGCGGTTCGCCGCGCCCG
CACAACCCTCCGGAGACAAC

Downstream 100 bases:

>100_bases
GGCTTTACCGCGCGCCAACGCGCTGGCAATCGTCAGTGCGACATGAACAGCGGCACGCCGGCGCGCCGCACCAGCGTGCG
CGTGGTGCCGCCCAGCACGC

Product: cysteine synthase

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKADNILQTIGNTPHIRIRRLFGNGHEVWIKSERSNPGGSIKDRIALSMVEDAERRGVLKPGGTIVEPTSGNTGIGLAMV
AAVKGYKLILVMPDSMSIERRRLMLAYGATFDLTPREKGMKGAIARAEELIASIPGAWMPQQFENPANIDVHARTTAQEI
LADFPEGLDALITGVGTGGHISGCAQVLKQKWPQLKVFAVEPTASPVISGGAPAPHPIQGIGAGFIPENLKTDLLDGVIQ
VDAEPAREMARRCAREEGILVGISSGATLAAIASKLPELPAGARVLGFNYDTGERYLTVEGFLPA

Sequences:

>Translated_305_residues
MKADNILQTIGNTPHIRIRRLFGNGHEVWIKSERSNPGGSIKDRIALSMVEDAERRGVLKPGGTIVEPTSGNTGIGLAMV
AAVKGYKLILVMPDSMSIERRRLMLAYGATFDLTPREKGMKGAIARAEELIASIPGAWMPQQFENPANIDVHARTTAQEI
LADFPEGLDALITGVGTGGHISGCAQVLKQKWPQLKVFAVEPTASPVISGGAPAPHPIQGIGAGFIPENLKTDLLDGVIQ
VDAEPAREMARRCAREEGILVGISSGATLAAIASKLPELPAGARVLGFNYDTGERYLTVEGFLPA
>Mature_305_residues
MKADNILQTIGNTPHIRIRRLFGNGHEVWIKSERSNPGGSIKDRIALSMVEDAERRGVLKPGGTIVEPTSGNTGIGLAMV
AAVKGYKLILVMPDSMSIERRRLMLAYGATFDLTPREKGMKGAIARAEELIASIPGAWMPQQFENPANIDVHARTTAQEI
LADFPEGLDALITGVGTGGHISGCAQVLKQKWPQLKVFAVEPTASPVISGGAPAPHPIQGIGAGFIPENLKTDLLDGVIQ
VDAEPAREMARRCAREEGILVGISSGATLAAIASKLPELPAGARVLGFNYDTGERYLTVEGFLPA

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=319, Percent_Identity=40.1253918495298, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI295821200, Length=319, Percent_Identity=40.1253918495298, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI4557415, Length=319, Percent_Identity=40.1253918495298, Blast_Score=206, Evalue=2e-53,
Organism=Escherichia coli, GI1788754, Length=310, Percent_Identity=51.2903225806452, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI2367138, Length=296, Percent_Identity=38.8513513513513, Blast_Score=193, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17535051, Length=295, Percent_Identity=45.0847457627119, Blast_Score=231, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI115535073, Length=300, Percent_Identity=48, Blast_Score=221, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17562970, Length=299, Percent_Identity=42.4749163879599, Blast_Score=221, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI32566674, Length=294, Percent_Identity=42.8571428571429, Blast_Score=218, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17561720, Length=299, Percent_Identity=42.4749163879599, Blast_Score=197, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI17534315, Length=311, Percent_Identity=38.9067524115756, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI25147552, Length=310, Percent_Identity=37.741935483871, Blast_Score=157, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI32566672, Length=213, Percent_Identity=39.4366197183099, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17561716, Length=119, Percent_Identity=40.3361344537815, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321594, Length=314, Percent_Identity=36.3057324840764, Blast_Score=181, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6321449, Length=323, Percent_Identity=33.4365325077399, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24643623, Length=305, Percent_Identity=37.0491803278689, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI20129101, Length=305, Percent_Identity=37.0491803278689, Blast_Score=166, Evalue=1e-41,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32416; Mature: 32416

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKADNILQTIGNTPHIRIRRLFGNGHEVWIKSERSNPGGSIKDRIALSMVEDAERRGVLK
CCHHHHHHHHCCCCCEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCC
PGGTIVEPTSGNTGIGLAMVAAVKGYKLILVMPDSMSIERRRLMLAYGATFDLTPREKGM
CCCEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHEEEEEECCEEECCCHHCCC
KGAIARAEELIASIPGAWMPQQFENPANIDVHARTTAQEILADFPEGLDALITGVGTGGH
HHHHHHHHHHHHHCCCCCCCHHCCCCCCEEEEEHHHHHHHHHHCCCCHHHHHHCCCCCCC
ISGCAQVLKQKWPQLKVFAVEPTASPVISGGAPAPHPIQGIGAGFIPENLKTDLLDGVIQ
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEE
VDAEPAREMARRCAREEGILVGISSGATLAAIASKLPELPAGARVLGFNYDTGERYLTVE
ECCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEE
GFLPA
CCCCC
>Mature Secondary Structure
MKADNILQTIGNTPHIRIRRLFGNGHEVWIKSERSNPGGSIKDRIALSMVEDAERRGVLK
CCHHHHHHHHCCCCCEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCC
PGGTIVEPTSGNTGIGLAMVAAVKGYKLILVMPDSMSIERRRLMLAYGATFDLTPREKGM
CCCEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHEEEEEECCEEECCCHHCCC
KGAIARAEELIASIPGAWMPQQFENPANIDVHARTTAQEILADFPEGLDALITGVGTGGH
HHHHHHHHHHHHHCCCCCCCHHCCCCCCEEEEEHHHHHHHHHHCCCCHHHHHHCCCCCCC
ISGCAQVLKQKWPQLKVFAVEPTASPVISGGAPAPHPIQGIGAGFIPENLKTDLLDGVIQ
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEE
VDAEPAREMARRCAREEGILVGISSGATLAAIASKLPELPAGARVLGFNYDTGERYLTVE
ECCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEE
GFLPA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8867384 [H]