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The map label for this gene is yafC [C]

Identifier: 73538553

GI number: 73538553

Start: 1427981

End: 1428880

Strand: Direct

Name: yafC [C]

Synonym: Reut_B4728

Alternate gene names: 73538553

Gene position: 1427981-1428880 (Clockwise)

Preceding gene: 73538552

Following gene: 73538554

Centisome position: 52.38

GC content: 64.78

Gene sequence:

>900_bases
GTGGATCGGCTGCAGGCCATGAAGACCTTCGTCACGGTCGTGGAGACCGGTGGCTTCACGGCCGCTGCGCGCAAGCTCGA
TGTATCGTTGTCCGTCGTGAGCCGTGTGATCACGGAACTGGAGGCCCACCTTGGCGTGCGGCTGCTGACGCGTACCACGC
GGCTGGTGCGGCCCACTGAGACCGGGACCGCTTACTTCGAAAATTGCCGACGCCTTCTGGCCGAGATTGAAGAGGCCGAA
CTTTCCGTCACGGGGACGCATGCGGCGCCGCGCGGCCAGTTGTCGGTGACCGCATCGGTCCTGTTCGGCTCGCGCTACGT
CACGCCGATCGTCGTCGAATATCTGCGGCGCTATCCCGAGGTCGGCGTCAGCTGCCTGCTGCTGGACCGCGTGGTCAACT
TTGTCGACGAGGGCATCGACGTCGGCGTGCGCATCGGCGAGTTGCCGAGTTCGTCGCTGCAGGCCGTTCCGGTCGGCCGC
GTGCGGCGCGTGGTGTGTGCATCGCCCGACTACCTCGCTCGCCATGGCGTGCCGCGAACACCGGATGACCTTGCCCACCA
CACGTTGATACAGACCACCGGCATCTCCACACTGCCGGAATGGCGCTTTCACCACAACGATCAACCGGTGTTCACCCGCA
TTGCCCCGCGCATGGCCACCACCACCAATGACTCCGCCATTGCCGCGGCAGTGGCAGGCTTCGGCGTGGCGCGGGTGCTG
TCGTACCAGATCGCTGGTGAACTGCGGGATGGGCGATTACAGACTGTGCTCGCCGACTATGAACCTGCGCCGCTTCCGAT
CCATGTGATCCATCGCGAAGGGCGCCACGCGGTGCAGAAAGTACGCGCGTTCCTCGATCTTGCGATCGAACGGCTGCGCG
CCGACAAGACCATCAACTGA

Upstream 100 bases:

>100_bases
TAAGCACCGGCACGCCGCCTGAGTTGCAAATACAGGAGGGGCTACGGCCCCTTTGCTGCATCCCGATATACTCGCGACAA
ACCAACCCCGGAGAGCACGC

Downstream 100 bases:

>100_bases
CGCCCCATTATTCTGGTTTCCAGAAGAGTCCCTTCACGGACAGGCCATTTCTCGTGGCCGTTCGCGCACCTAAGATGGTT
TGCAGCAGAACATCCCGCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MDRLQAMKTFVTVVETGGFTAAARKLDVSLSVVSRVITELEAHLGVRLLTRTTRLVRPTETGTAYFENCRRLLAEIEEAE
LSVTGTHAAPRGQLSVTASVLFGSRYVTPIVVEYLRRYPEVGVSCLLLDRVVNFVDEGIDVGVRIGELPSSSLQAVPVGR
VRRVVCASPDYLARHGVPRTPDDLAHHTLIQTTGISTLPEWRFHHNDQPVFTRIAPRMATTTNDSAIAAAVAGFGVARVL
SYQIAGELRDGRLQTVLADYEPAPLPIHVIHREGRHAVQKVRAFLDLAIERLRADKTIN

Sequences:

>Translated_299_residues
MDRLQAMKTFVTVVETGGFTAAARKLDVSLSVVSRVITELEAHLGVRLLTRTTRLVRPTETGTAYFENCRRLLAEIEEAE
LSVTGTHAAPRGQLSVTASVLFGSRYVTPIVVEYLRRYPEVGVSCLLLDRVVNFVDEGIDVGVRIGELPSSSLQAVPVGR
VRRVVCASPDYLARHGVPRTPDDLAHHTLIQTTGISTLPEWRFHHNDQPVFTRIAPRMATTTNDSAIAAAVAGFGVARVL
SYQIAGELRDGRLQTVLADYEPAPLPIHVIHREGRHAVQKVRAFLDLAIERLRADKTIN
>Mature_299_residues
MDRLQAMKTFVTVVETGGFTAAARKLDVSLSVVSRVITELEAHLGVRLLTRTTRLVRPTETGTAYFENCRRLLAEIEEAE
LSVTGTHAAPRGQLSVTASVLFGSRYVTPIVVEYLRRYPEVGVSCLLLDRVVNFVDEGIDVGVRIGELPSSSLQAVPVGR
VRRVVCASPDYLARHGVPRTPDDLAHHTLIQTTGISTLPEWRFHHNDQPVFTRIAPRMATTTNDSAIAAAVAGFGVARVL
SYQIAGELRDGRLQTVLADYEPAPLPIHVIHREGRHAVQKVRAFLDLAIERLRADKTIN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=32.4137931034483, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1789639, Length=289, Percent_Identity=31.4878892733564, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI145693193, Length=288, Percent_Identity=29.5138888888889, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787128, Length=280, Percent_Identity=30.3571428571429, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787589, Length=285, Percent_Identity=29.1228070175439, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1789440, Length=288, Percent_Identity=26.0416666666667, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI87081978, Length=262, Percent_Identity=29.3893129770992, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1788706, Length=261, Percent_Identity=25.2873563218391, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=24.406779661017, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786448, Length=246, Percent_Identity=27.2357723577236, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI157672245, Length=156, Percent_Identity=28.8461538461538, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788748, Length=158, Percent_Identity=33.5443037974684, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1790011, Length=301, Percent_Identity=22.2591362126246, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788297, Length=116, Percent_Identity=31.0344827586207, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32900; Mature: 32900

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLQAMKTFVTVVETGGFTAAARKLDVSLSVVSRVITELEAHLGVRLLTRTTRLVRPTE
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TGTAYFENCRRLLAEIEEAELSVTGTHAAPRGQLSVTASVLFGSRYVTPIVVEYLRRYPE
CCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEHHHHHCCCCHHHHHHHHHHHCHH
VGVSCLLLDRVVNFVDEGIDVGVRIGELPSSSLQAVPVGRVRRVVCASPDYLARHGVPRT
HHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCHHHHCCCCCC
PDDLAHHTLIQTTGISTLPEWRFHHNDQPVFTRIAPRMATTTNDSAIAAAVAGFGVARVL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHH
SYQIAGELRDGRLQTVLADYEPAPLPIHVIHREGRHAVQKVRAFLDLAIERLRADKTIN
HHHHHHCCCCCHHHEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDRLQAMKTFVTVVETGGFTAAARKLDVSLSVVSRVITELEAHLGVRLLTRTTRLVRPTE
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TGTAYFENCRRLLAEIEEAELSVTGTHAAPRGQLSVTASVLFGSRYVTPIVVEYLRRYPE
CCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEHHHHHCCCCHHHHHHHHHHHCHH
VGVSCLLLDRVVNFVDEGIDVGVRIGELPSSSLQAVPVGRVRRVVCASPDYLARHGVPRT
HHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCHHHHCCCCCC
PDDLAHHTLIQTTGISTLPEWRFHHNDQPVFTRIAPRMATTTNDSAIAAAVAGFGVARVL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHH
SYQIAGELRDGRLQTVLADYEPAPLPIHVIHREGRHAVQKVRAFLDLAIERLRADKTIN
HHHHHHCCCCCHHHEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]