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The map label for this gene is mdlA [H]

Identifier: 73538470

GI number: 73538470

Start: 1329721

End: 1330986

Strand: Direct

Name: mdlA [H]

Synonym: Reut_B4643

Alternate gene names: 73538470

Gene position: 1329721-1330986 (Clockwise)

Preceding gene: 73538468

Following gene: 73538471

Centisome position: 48.78

GC content: 65.24

Gene sequence:

>1266_bases
ATGACGTTTGGATTATATCAGTCACGCACACCCAAAAATGATAACGTTATCATTAACGCCCAGAGGCAATTCCGTACCTC
AATGTCTGCCCGATCAAAATTTCCCTGCCCCACGCCCATGACCACCCCTGCCACTCCTTCCACCGACACCATCGCCACGA
TCCGGCTTTCCTCGTGCTACTTGCCATTGGCCACACCGATCAGCGATGCCAAGGTCCTGACCGGTCGCCAAAAGCCAATG
ACCGAGGTCGCGATCCTGTTTGCCGAGATCCGGACCCACGGCGGCCACGAAGGTATCGGTTTCAGCTATGCCAAGCGCGC
CGGAGGACCGGGCCAGTTCGCGCATGCCAGCGAAATCGCACCGGCCCTGCTCGGTGAGGACCCGAGCGACATCGCCCGGC
TGTGGGACAAGCTCTGCTGGGCCGGCGCGTCGGTCGGACGCAGCGGATTGTCGACACAGGCAATGGGCGCGTTCGATGTG
GCACTCTGGGACCTGAAAGCCAAGCGGGCAGGCTTGTCGCTGGCCAAGCTGCTGGGGGCCCATCGCGACGCCGTGCGCTG
CTACAACACCTCGGGCGGTTTCCTGCACACGCCGCTGGACCAGTTGCTGGTCAACGCAGCGGCCTCGGTGGAGCGCGGGA
TCGGCGGGATCAAGCTGAAGGTCGGCCAGCCTGATGGTGCGCTGGACATCCAGCGCGTGGCCGCCGTGCGCGAACAGCTT
GGCGATGCCGTGCCGCTGATGGTCGACGCCAACCAGCAATGGGATCGGCCGACGGCGCAACGCATGTGCCGCACGTTCGA
ATCCTTCAACCTGGTCTGGATCGAGGAGCCGCTGGACGCCTACGACCACGAGGGGCACGCCGCGCTCGCCACGCAGTTCG
ACACGCCGATCGCGACCGGCGAAATGCTGACCAGCGCGGCCGAACACTGGGACCTGATCCGGCACCGCGCCGCGGACTAC
CTGATGCCGGACGCGCCGCGCGTTGGCGGCATTACGCCCTTTCTCAAGGTCTGCGCGCTGGCCGAGCATGCGGGCCTGAT
GATCGCGCCGCACTTCGCCATGGAGTTGCACGTGCACCTTGCCGCAGCCTATCCACGCGAGCCCTGGGTCGAGCACTTCG
ACTGGCTAGAACCGCTGTTCAACGAACGGCTGGAGATACGCGGCGGCCGCATCGTGGTGCCGACCCGGCCGGGCCTGGGA
CTCACCATCAGCGAGCAGGCCCGCGCGTGGACGCGCGAACAGGCCGAATTCGGCCAACAAGCCTGA

Upstream 100 bases:

>100_bases
GCGATTGACGATCGAGAATGACAACGTTAGCATTTCTATCATTCCTTCGTCAGCGACCTGTCGCAGCCGAGCGGAACCAG
GATGTTGAACCCCGGCGGAG

Downstream 100 bases:

>100_bases
CAGGCGTCGGAATCCCGCATACACAGAACGGTGAAAGGAGACAAAAGATGAAAAAGCTGATTGCGTCCGTACTCACGACG
ACTGCCATGACCGTTGCCAT

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: MR [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MTFGLYQSRTPKNDNVIINAQRQFRTSMSARSKFPCPTPMTTPATPSTDTIATIRLSSCYLPLATPISDAKVLTGRQKPM
TEVAILFAEIRTHGGHEGIGFSYAKRAGGPGQFAHASEIAPALLGEDPSDIARLWDKLCWAGASVGRSGLSTQAMGAFDV
ALWDLKAKRAGLSLAKLLGAHRDAVRCYNTSGGFLHTPLDQLLVNAAASVERGIGGIKLKVGQPDGALDIQRVAAVREQL
GDAVPLMVDANQQWDRPTAQRMCRTFESFNLVWIEEPLDAYDHEGHAALATQFDTPIATGEMLTSAAEHWDLIRHRAADY
LMPDAPRVGGITPFLKVCALAEHAGLMIAPHFAMELHVHLAAAYPREPWVEHFDWLEPLFNERLEIRGGRIVVPTRPGLG
LTISEQARAWTREQAEFGQQA

Sequences:

>Translated_421_residues
MTFGLYQSRTPKNDNVIINAQRQFRTSMSARSKFPCPTPMTTPATPSTDTIATIRLSSCYLPLATPISDAKVLTGRQKPM
TEVAILFAEIRTHGGHEGIGFSYAKRAGGPGQFAHASEIAPALLGEDPSDIARLWDKLCWAGASVGRSGLSTQAMGAFDV
ALWDLKAKRAGLSLAKLLGAHRDAVRCYNTSGGFLHTPLDQLLVNAAASVERGIGGIKLKVGQPDGALDIQRVAAVREQL
GDAVPLMVDANQQWDRPTAQRMCRTFESFNLVWIEEPLDAYDHEGHAALATQFDTPIATGEMLTSAAEHWDLIRHRAADY
LMPDAPRVGGITPFLKVCALAEHAGLMIAPHFAMELHVHLAAAYPREPWVEHFDWLEPLFNERLEIRGGRIVVPTRPGLG
LTISEQARAWTREQAEFGQQA
>Mature_420_residues
TFGLYQSRTPKNDNVIINAQRQFRTSMSARSKFPCPTPMTTPATPSTDTIATIRLSSCYLPLATPISDAKVLTGRQKPMT
EVAILFAEIRTHGGHEGIGFSYAKRAGGPGQFAHASEIAPALLGEDPSDIARLWDKLCWAGASVGRSGLSTQAMGAFDVA
LWDLKAKRAGLSLAKLLGAHRDAVRCYNTSGGFLHTPLDQLLVNAAASVERGIGGIKLKVGQPDGALDIQRVAAVREQLG
DAVPLMVDANQQWDRPTAQRMCRTFESFNLVWIEEPLDAYDHEGHAALATQFDTPIATGEMLTSAAEHWDLIRHRAADYL
MPDAPRVGGITPFLKVCALAEHAGLMIAPHFAMELHVHLAAAYPREPWVEHFDWLEPLFNERLEIRGGRIVVPTRPGLGL
TISEQARAWTREQAEFGQQA

Specific function: D-galactonate degradation; first step. D-galactonate degradation; third step. [C]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=395, Percent_Identity=25.0632911392405, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI186972148, Length=234, Percent_Identity=28.6324786324786, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI42544117, Length=392, Percent_Identity=24.234693877551, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI48994953, Length=317, Percent_Identity=28.391167192429, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI226510960, Length=376, Percent_Identity=26.8617021276596, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.1.2.2 [H]

Molecular weight: Translated: 45974; Mature: 45843

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFGLYQSRTPKNDNVIINAQRQFRTSMSARSKFPCPTPMTTPATPSTDTIATIRLSSCY
CCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
LPLATPISDAKVLTGRQKPMTEVAILFAEIRTHGGHEGIGFSYAKRAGGPGQFAHASEIA
CCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHH
PALLGEDPSDIARLWDKLCWAGASVGRSGLSTQAMGAFDVALWDLKAKRAGLSLAKLLGA
HHHHCCCHHHHHHHHHHHHHCCHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHC
HRDAVRCYNTSGGFLHTPLDQLLVNAAASVERGIGGIKLKVGQPDGALDIQRVAAVREQL
CCCHHEEECCCCCEEECCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
GDAVPLMVDANQQWDRPTAQRMCRTFESFNLVWIEEPLDAYDHEGHAALATQFDTPIATG
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCEEEEECCHHHCCCCCCEEEEEECCCCCCHH
EMLTSAAEHWDLIRHRAADYLMPDAPRVGGITPFLKVCALAEHAGLMIAPHFAMELHVHL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCEEEECCHHEEEEEEE
AAAYPREPWVEHFDWLEPLFNERLEIRGGRIVVPTRPGLGLTISEQARAWTREQAEFGQQ
EECCCCCHHHHHHHHHHHHHCCCEEECCCEEEEECCCCCCEEECHHHHHHHHHHHHCCCC
A
C
>Mature Secondary Structure 
TFGLYQSRTPKNDNVIINAQRQFRTSMSARSKFPCPTPMTTPATPSTDTIATIRLSSCY
CCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
LPLATPISDAKVLTGRQKPMTEVAILFAEIRTHGGHEGIGFSYAKRAGGPGQFAHASEIA
CCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHH
PALLGEDPSDIARLWDKLCWAGASVGRSGLSTQAMGAFDVALWDLKAKRAGLSLAKLLGA
HHHHCCCHHHHHHHHHHHHHCCHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHC
HRDAVRCYNTSGGFLHTPLDQLLVNAAASVERGIGGIKLKVGQPDGALDIQRVAAVREQL
CCCHHEEECCCCCEEECCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
GDAVPLMVDANQQWDRPTAQRMCRTFESFNLVWIEEPLDAYDHEGHAALATQFDTPIATG
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCEEEEECCHHHCCCCCCEEEEEECCCCCCHH
EMLTSAAEHWDLIRHRAADYLMPDAPRVGGITPFLKVCALAEHAGLMIAPHFAMELHVHL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCEEEECCHHEEEEEEE
AAAYPREPWVEHFDWLEPLFNERLEIRGGRIVVPTRPGLGLTISEQARAWTREQAEFGQQ
EECCCCCHHHHHHHHHHHHHCCCEEECCCEEEEECCCCCCEEECHHHHHHHHHHHHCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2831968; 2271624; 2215699; 1909893; 1892834; 7893689; 7893690 [H]