BacMap

Definition	Ralstonia eutropha JMP134 chromosome chromosome 2, complete sequence.
Accession	NC_007348
Length	2,726,152

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The map label for this gene is benD [H]

Identifier: 73538235

GI number: 73538235

Start: 1063611

End: 1064396

Strand: Direct

Name: benD [H]

Synonym: Reut_B4406

Alternate gene names: 73538235

Gene position: 1063611-1064396 (Clockwise)

Preceding gene: 73538234

Following gene: 73538240

Centisome position: 39.02

GC content: 66.03

Gene sequence:

>786_bases
ATGAGCACTTCCAATCGTTTTGAAGGCAAGATCGTCGTCATCACCGGCGCGGCACAGGGCATCGGCCGCGGCGTGGCGCT
GCGCGCAGCGGCCGAGGGAGCCATCCTCGTGCTCGCTGACCGGTCGGAACTCGTCCATGAGGTTGTGGCCGAAATTGCCG
CGCAGGACGGCCGCGCCACGGCGGTGATCGTCGACCTGGAAACCTATGCCGGCGCCAGGGCCATGATCGATACGGCACTC
TCTGCGCACGGTCGTGTCGACGTGCTCATCAACAATGTCGGCGGCACCATCTGGGCCAAGCCTTACCAGCACTACGAAGC
AGAGCAGATCGAAGCCGAGATCCGCCGCTCGCTGTTCCCCACGCTGTGGAGCTGCCGCGCGGCGCTGCCCGGCATGGTCG
AGCGGCGCCAGGGCACAATCGTCAATGTGTCGTCGATCGCTACACGCAGTATCCACCGCGTGCCGTATGCCGCGGCCAAG
GGCGGCGTCAATGCGCTGACTGCTAGCCTTGCGTTCGAACACGCCGGCGAAGGTATCCGCGTCAATGCGGTCGCGACCGG
CGGCACGGAAGCCCCGCCGCGCCGCATCCCGCGCAATACGTCCGAGCAAACGCCGCAGGAAGCCGAGTGGTACCAGGGCA
TCGTCGATCAGACCATCGGCTCGAGCCTGATGCACCGCTACGGCACCATCGACGAGCAGGTCGGCGCGATCCTGTTCCTG
GCGTCGGACGAGGCGTCCTACATTACCGGGACGGTGCTGCCGGTTGGTGGCGGAGATCAGGGCTAA

Upstream 100 bases:

>100_bases
CAATGGTCGATGCGGTGCGGAACTGGCTCAAGGAAAGCGGCGTCGAGCCGGCAGGCTTCTACTACGAAAAATTCTCGGCC
AGCAGCGTGGCCTGACTGTC

Downstream 100 bases:

>100_bases
GCCGTTTGTCCTGCGTGGGGTGGCGTCCGTCGCTGCCCGCGCTGTCCAGCCAGTGGCGCCATGGCGCCCTGGCGTCTTTT
TGATCTTGACCTCGGCAGCT

Product: 1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase

Products: NA

Alternate protein names: 2-hydro-1,2-dihydroxybenzoate dehydrogenase; DHB dehydrogenase; Cis-1,2-dihydroxy-3,4-cyclohexadiene-1-carboxylate dehydrogenase; Cis-1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate dehydrogenase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSTSNRFEGKIVVITGAAQGIGRGVALRAAAEGAILVLADRSELVHEVVAEIAAQDGRATAVIVDLETYAGARAMIDTAL
SAHGRVDVLINNVGGTIWAKPYQHYEAEQIEAEIRRSLFPTLWSCRAALPGMVERRQGTIVNVSSIATRSIHRVPYAAAK
GGVNALTASLAFEHAGEGIRVNAVATGGTEAPPRRIPRNTSEQTPQEAEWYQGIVDQTIGSSLMHRYGTIDEQVGAILFL
ASDEASYITGTVLPVGGGDQG

Sequences:

>Translated_261_residues
MSTSNRFEGKIVVITGAAQGIGRGVALRAAAEGAILVLADRSELVHEVVAEIAAQDGRATAVIVDLETYAGARAMIDTAL
SAHGRVDVLINNVGGTIWAKPYQHYEAEQIEAEIRRSLFPTLWSCRAALPGMVERRQGTIVNVSSIATRSIHRVPYAAAK
GGVNALTASLAFEHAGEGIRVNAVATGGTEAPPRRIPRNTSEQTPQEAEWYQGIVDQTIGSSLMHRYGTIDEQVGAILFL
ASDEASYITGTVLPVGGGDQG
>Mature_260_residues
STSNRFEGKIVVITGAAQGIGRGVALRAAAEGAILVLADRSELVHEVVAEIAAQDGRATAVIVDLETYAGARAMIDTALS
AHGRVDVLINNVGGTIWAKPYQHYEAEQIEAEIRRSLFPTLWSCRAALPGMVERRQGTIVNVSSIATRSIHRVPYAAAKG
GVNALTASLAFEHAGEGIRVNAVATGGTEAPPRRIPRNTSEQTPQEAEWYQGIVDQTIGSSLMHRYGTIDEQVGAILFLA
SDEASYITGTVLPVGGGDQG

Specific function: Degradation of 2-hydro-1,2-dihydroxy benzoate (DHB) to catechol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI19923817, Length=265, Percent_Identity=27.1698113207547, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI32483357, Length=262, Percent_Identity=27.0992366412214, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI66933014, Length=256, Percent_Identity=28.90625, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI15277342, Length=265, Percent_Identity=26.7924528301887, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI126723750, Length=267, Percent_Identity=26.2172284644195, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI7705925, Length=255, Percent_Identity=29.0196078431373, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI4504505, Length=197, Percent_Identity=30.9644670050761, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI59889578, Length=234, Percent_Identity=27.7777777777778, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI10190704, Length=257, Percent_Identity=30.3501945525292, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI210032110, Length=201, Percent_Identity=30.3482587064677, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI40807363, Length=173, Percent_Identity=31.2138728323699, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI40254986, Length=196, Percent_Identity=26.530612244898, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI20149619, Length=190, Percent_Identity=33.6842105263158, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI87082100, Length=264, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI1787335, Length=254, Percent_Identity=31.496062992126, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI2367175, Length=257, Percent_Identity=32.295719844358, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787905, Length=266, Percent_Identity=30.8270676691729, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789208, Length=255, Percent_Identity=28.2352941176471, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1790717, Length=262, Percent_Identity=26.7175572519084, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1789378, Length=263, Percent_Identity=28.1368821292776, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788459, Length=256, Percent_Identity=27.34375, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787820, Length=224, Percent_Identity=28.125, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71994600, Length=257, Percent_Identity=33.0739299610895, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71994604, Length=254, Percent_Identity=30.3149606299213, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17559104, Length=272, Percent_Identity=31.25, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17562910, Length=274, Percent_Identity=31.3868613138686, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17544670, Length=193, Percent_Identity=29.0155440414508, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17565030, Length=270, Percent_Identity=29.2592592592593, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI72000259, Length=190, Percent_Identity=28.4210526315789, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17562904, Length=195, Percent_Identity=30.7692307692308, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17561402, Length=268, Percent_Identity=26.865671641791, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17555706, Length=263, Percent_Identity=30.7984790874525, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538480, Length=271, Percent_Identity=29.520295202952, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17560332, Length=266, Percent_Identity=26.3157894736842, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17560150, Length=189, Percent_Identity=30.1587301587302, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25147288, Length=256, Percent_Identity=29.6875, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17563726, Length=189, Percent_Identity=29.1005291005291, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560676, Length=252, Percent_Identity=25.3968253968254, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17562990, Length=266, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115534694, Length=267, Percent_Identity=28.0898876404494, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17564282, Length=169, Percent_Identity=31.3609467455621, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17531453, Length=269, Percent_Identity=28.2527881040892, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI193204405, Length=194, Percent_Identity=29.8969072164948, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI193203117, Length=171, Percent_Identity=32.1637426900585, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193203115, Length=168, Percent_Identity=32.7380952380952, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17551412, Length=193, Percent_Identity=31.0880829015544, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71982365, Length=196, Percent_Identity=28.0612244897959, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17538486, Length=269, Percent_Identity=26.0223048327138, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17568967, Length=213, Percent_Identity=29.1079812206573, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=195, Percent_Identity=27.1794871794872, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=195, Percent_Identity=27.1794871794872, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17560220, Length=275, Percent_Identity=26.1818181818182, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=191, Percent_Identity=32.4607329842932, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324126, Length=268, Percent_Identity=23.5074626865672, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24644339, Length=263, Percent_Identity=34.6007604562738, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28571526, Length=260, Percent_Identity=33.0769230769231, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI23397609, Length=262, Percent_Identity=31.6793893129771, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21357041, Length=256, Percent_Identity=31.640625, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21355319, Length=271, Percent_Identity=26.9372693726937, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24639444, Length=265, Percent_Identity=27.1698113207547, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24644337, Length=265, Percent_Identity=31.6981132075472, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24640492, Length=203, Percent_Identity=27.5862068965517, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281364892, Length=169, Percent_Identity=33.1360946745562, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21357673, Length=197, Percent_Identity=26.3959390862944, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI18859837, Length=200, Percent_Identity=31, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI21358495, Length=193, Percent_Identity=27.979274611399, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.25 [H]

Molecular weight: Translated: 27573; Mature: 27442

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSNRFEGKIVVITGAAQGIGRGVALRAAAEGAILVLADRSELVHEVVAEIAAQDGRAT
CCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHCCCCEE
AVIVDLETYAGARAMIDTALSAHGRVDVLINNVGGTIWAKPYQHYEAEQIEAEIRRSLFP
EEEEEEHHHCCHHHHHHHHHHCCCEEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHH
TLWSCRAALPGMVERRQGTIVNVSSIATRSIHRVPYAAAKGGVNALTASLAFEHAGEGIR
HHHHHHHHCCCHHHHCCCCEEEHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCEE
VNAVATGGTEAPPRRIPRNTSEQTPQEAEWYQGIVDQTIGSSLMHRYGTIDEQVGAILFL
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
ASDEASYITGTVLPVGGGDQG
ECCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure 
STSNRFEGKIVVITGAAQGIGRGVALRAAAEGAILVLADRSELVHEVVAEIAAQDGRAT
CCCCCCCCEEEEEECCCCCCCCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHCCCCEE
AVIVDLETYAGARAMIDTALSAHGRVDVLINNVGGTIWAKPYQHYEAEQIEAEIRRSLFP
EEEEEEHHHCCHHHHHHHHHHCCCEEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHH
TLWSCRAALPGMVERRQGTIVNVSSIATRSIHRVPYAAAKGGVNALTASLAFEHAGEGIR
HHHHHHHHCCCHHHHCCCCEEEHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCEE
VNAVATGGTEAPPRRIPRNTSEQTPQEAEWYQGIVDQTIGSSLMHRYGTIDEQVGAILFL
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
ASDEASYITGTVLPVGGGDQG
ECCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1740120 [H]