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The map label for this gene is acsA [H]

Identifier: 73538200

GI number: 73538200

Start: 1027623

End: 1029284

Strand: Reverse

Name: acsA [H]

Synonym: Reut_B4371

Alternate gene names: 73538200

Gene position: 1029284-1027623 (Counterclockwise)

Preceding gene: 73538203

Following gene: 73538199

Centisome position: 37.76

GC content: 67.93

Gene sequence:

>1662_bases
ATGCGCGATTACACCGAGACCTTTGCCGGATTTTCGTACCAGGACACCGTCAGCCGTCAGTTGCACGGCAGCCTGGATGC
CATGAACGCCTGCGTGGAATGCTGCGACCGGCACGCGCTGCCGGGCCGCATCGCGCTGTTCTGGGAGGGGCGCGACGGAA
CGTCCGAAACCTGGACGTTCGCCCAGCTCAAGGCCCTGTCCGCAAAAGTCGCGGGCTTCCTGAAGGCCCAAGGCGTGCAA
CCCGGTGATCGCGTGGCGGGTTTGCTGCCGCGCACGCCGGAACTGCTGGCCACCATCCTCGGCACCTGGCGCGCTGGCGC
GGTCTACCAGCCGCTGTTTACGGCGTTCGGCCCGAAGGCCATCGAACACCGGCTCAATGCGTCGGGTGCGAAGTTCGTGG
TGACCGACGGCGCCAATCGCAGCAAGCTGGACGACGTTGCCGACTGCCCGCCCATCGTCACCGTGGCCGGCGCCAAGGGC
CGCGGCCTTGTGCGTGGGGACTTCAGCTTCTGGGCCGAAGTGGACCGCCAGCCCGCCGAATTCGAGCCCGTCATGCGCCG
TGGCGACGACCCGTTCCTGATGATGTTCACGTCGGGCACCACGGGCCCGGCCAAGCCGTTGCAGGTGCCGCTGAAGGCCA
TCGTCGCGTTCGCGGGCTATATGCGCGATGCGGTGGACCTGCGCGACGCGGACGCGTTCTGGAACCTGGCCGATCCCGGC
TGGGCCTATGGCCTGTACTACGCGGTCACGGGCCCGCTGGCGCTGGGCCATCCGACCACGTTCTACGACGGCCCGTTCAG
CGTGGAAAGCACGTGCCGCGTGATCGAGAAATACGGCATCACCAACCTGGCGGGCTCGCCCACCGCATACCGGCTGCTGA
TCGCGGCCGGCAAGGCGGTATCGGGCCCGCTGCGCGGCAAGCTGCGCGCGGTCAGCAGCGCGGGCGAGCCGCTCAACCCC
GAAGTCATCCGCTGGTTCGCCAATGAGCTGGGCGTGACCATTCACGACCACTACGGCCAGACCGAAACCGGCATGGTGCT
GTGCAACCACCATGCGCTGTCGCATACGGTGCGCATGGGCGCCGCGGGCTTTGCCAGCCCGGGCCACCGCGTGGTCGTGC
TTGACGACGAGAACCGCGAACTGCCGGCTGGCCAGCCGGGCACGCTGGCGCTGGATCTCACGCAGTCGCCGATGTGCTGG
TTCCCCGGCTACCACGGCACGCCGACGCGCGCCTTCGTCGGCAACTACTACCTGACCGGCGATTCGGCCGAACTCAACGA
CGACGGCAGCATCAGCTTTATCGGCCGGGCGGACGACGTGATCACGACCTCGGGCTACCGGGTCGGGCCGTTCGACGTCG
AAAGCGCGCTGATCGAGCATCCGGCCGTGGTCGAGACGGCCGTGATCGGCAAGCCCGATCCCGAGCGCACCGAGCTGATC
AAGGCCTTCGTCGTGCTCGATCCGCAATACCGGCCTACGCCAGAACTGGCCGAGGAGTTGCGCCTGCACGTGCGCAAGCG
CCTGGCGGCACACGCCTACCCGCGCGAAATCGAATTCGTGCCGGAACTGCCCAAGACCCCCAGCGGCAAGGTGCAGCGCT
TTATCCTGCGCAACCAGGAGGTCGCCAAGGCACGCGAAGCGCAGGGTGCGCAGGCCGCCTGA

Upstream 100 bases:

>100_bases
GGCTATTGCCGTGTGCAAAGCGGTGAACCACACTGCTGCAGCCAGGCGCTTGCCTCGACGCAGCGCGCCGTAGAACACAG
ATGACAAGGTTGGAGACATC

Downstream 100 bases:

>100_bases
TCGCCACGCAAACGCCGGCACAGCGCTTCCTATAATGGGGAGGCGGTGCCGGCCGTCTCCTGGTCATTGGTAGCAGGCGG
GCGCCGCGCCAGGCGTGCCT

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Acetate--CoA ligase; Acetyl-CoA synthase; Acyl-activating enzyme [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MRDYTETFAGFSYQDTVSRQLHGSLDAMNACVECCDRHALPGRIALFWEGRDGTSETWTFAQLKALSAKVAGFLKAQGVQ
PGDRVAGLLPRTPELLATILGTWRAGAVYQPLFTAFGPKAIEHRLNASGAKFVVTDGANRSKLDDVADCPPIVTVAGAKG
RGLVRGDFSFWAEVDRQPAEFEPVMRRGDDPFLMMFTSGTTGPAKPLQVPLKAIVAFAGYMRDAVDLRDADAFWNLADPG
WAYGLYYAVTGPLALGHPTTFYDGPFSVESTCRVIEKYGITNLAGSPTAYRLLIAAGKAVSGPLRGKLRAVSSAGEPLNP
EVIRWFANELGVTIHDHYGQTETGMVLCNHHALSHTVRMGAAGFASPGHRVVVLDDENRELPAGQPGTLALDLTQSPMCW
FPGYHGTPTRAFVGNYYLTGDSAELNDDGSISFIGRADDVITTSGYRVGPFDVESALIEHPAVVETAVIGKPDPERTELI
KAFVVLDPQYRPTPELAEELRLHVRKRLAAHAYPREIEFVPELPKTPSGKVQRFILRNQEVAKAREAQGAQAA

Sequences:

>Translated_553_residues
MRDYTETFAGFSYQDTVSRQLHGSLDAMNACVECCDRHALPGRIALFWEGRDGTSETWTFAQLKALSAKVAGFLKAQGVQ
PGDRVAGLLPRTPELLATILGTWRAGAVYQPLFTAFGPKAIEHRLNASGAKFVVTDGANRSKLDDVADCPPIVTVAGAKG
RGLVRGDFSFWAEVDRQPAEFEPVMRRGDDPFLMMFTSGTTGPAKPLQVPLKAIVAFAGYMRDAVDLRDADAFWNLADPG
WAYGLYYAVTGPLALGHPTTFYDGPFSVESTCRVIEKYGITNLAGSPTAYRLLIAAGKAVSGPLRGKLRAVSSAGEPLNP
EVIRWFANELGVTIHDHYGQTETGMVLCNHHALSHTVRMGAAGFASPGHRVVVLDDENRELPAGQPGTLALDLTQSPMCW
FPGYHGTPTRAFVGNYYLTGDSAELNDDGSISFIGRADDVITTSGYRVGPFDVESALIEHPAVVETAVIGKPDPERTELI
KAFVVLDPQYRPTPELAEELRLHVRKRLAAHAYPREIEFVPELPKTPSGKVQRFILRNQEVAKAREAQGAQAA
>Mature_553_residues
MRDYTETFAGFSYQDTVSRQLHGSLDAMNACVECCDRHALPGRIALFWEGRDGTSETWTFAQLKALSAKVAGFLKAQGVQ
PGDRVAGLLPRTPELLATILGTWRAGAVYQPLFTAFGPKAIEHRLNASGAKFVVTDGANRSKLDDVADCPPIVTVAGAKG
RGLVRGDFSFWAEVDRQPAEFEPVMRRGDDPFLMMFTSGTTGPAKPLQVPLKAIVAFAGYMRDAVDLRDADAFWNLADPG
WAYGLYYAVTGPLALGHPTTFYDGPFSVESTCRVIEKYGITNLAGSPTAYRLLIAAGKAVSGPLRGKLRAVSSAGEPLNP
EVIRWFANELGVTIHDHYGQTETGMVLCNHHALSHTVRMGAAGFASPGHRVVVLDDENRELPAGQPGTLALDLTQSPMCW
FPGYHGTPTRAFVGNYYLTGDSAELNDDGSISFIGRADDVITTSGYRVGPFDVESALIEHPAVVETAVIGKPDPERTELI
KAFVVLDPQYRPTPELAEELRLHVRKRLAAHAYPREIEFVPELPKTPSGKVQRFILRNQEVAKAREAQGAQAA

Specific function: Has a role in growth and sporulation on acetate [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=536, Percent_Identity=36.3805970149254, Blast_Score=295, Evalue=7e-80,
Organism=Homo sapiens, GI122937307, Length=510, Percent_Identity=36.6666666666667, Blast_Score=292, Evalue=7e-79,
Organism=Homo sapiens, GI42544132, Length=516, Percent_Identity=34.8837209302326, Blast_Score=286, Evalue=3e-77,
Organism=Homo sapiens, GI115511026, Length=511, Percent_Identity=33.4637964774951, Blast_Score=273, Evalue=3e-73,
Organism=Homo sapiens, GI58082049, Length=504, Percent_Identity=34.7222222222222, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI157311624, Length=512, Percent_Identity=33.7890625, Blast_Score=263, Evalue=3e-70,
Organism=Homo sapiens, GI157311622, Length=512, Percent_Identity=33.7890625, Blast_Score=263, Evalue=3e-70,
Organism=Homo sapiens, GI8923896, Length=575, Percent_Identity=29.9130434782609, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI28416953, Length=559, Percent_Identity=32.2003577817531, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI253970489, Length=594, Percent_Identity=29.4612794612795, Blast_Score=220, Evalue=3e-57,
Organism=Homo sapiens, GI13375727, Length=557, Percent_Identity=28.7253141831239, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI42544134, Length=380, Percent_Identity=28.1578947368421, Blast_Score=137, Evalue=4e-32,
Organism=Homo sapiens, GI195947378, Length=443, Percent_Identity=26.86230248307, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI156151445, Length=576, Percent_Identity=24.8263888888889, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI38524616, Length=552, Percent_Identity=25.7246376811594, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI187761345, Length=492, Percent_Identity=25.609756097561, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI187761343, Length=492, Percent_Identity=25.609756097561, Blast_Score=86, Evalue=9e-17,
Organism=Escherichia coli, GI1790505, Length=547, Percent_Identity=31.9926873857404, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI145693145, Length=516, Percent_Identity=26.3565891472868, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1786529, Length=566, Percent_Identity=28.2685512367491, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1788107, Length=535, Percent_Identity=26.5420560747664, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI221142682, Length=525, Percent_Identity=23.6190476190476, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1786810, Length=530, Percent_Identity=23.7735849056604, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786801, Length=443, Percent_Identity=22.7990970654628, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71983001, Length=568, Percent_Identity=31.5140845070423, Blast_Score=218, Evalue=8e-57,
Organism=Caenorhabditis elegans, GI71982997, Length=568, Percent_Identity=31.5140845070423, Blast_Score=217, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17557194, Length=541, Percent_Identity=23.8447319778189, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32563687, Length=529, Percent_Identity=24.5746691871456, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17538037, Length=550, Percent_Identity=23.6363636363636, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17558820, Length=470, Percent_Identity=24.0425531914894, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71994703, Length=515, Percent_Identity=23.495145631068, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71994690, Length=515, Percent_Identity=23.495145631068, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71994694, Length=515, Percent_Identity=23.495145631068, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17559526, Length=547, Percent_Identity=24.4972577696526, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17560308, Length=365, Percent_Identity=25.4794520547945, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71985884, Length=404, Percent_Identity=24.5049504950495, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=507, Percent_Identity=21.6962524654832, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319264, Length=563, Percent_Identity=32.5044404973357, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6323182, Length=589, Percent_Identity=29.2020373514431, Blast_Score=199, Evalue=8e-52,
Organism=Saccharomyces cerevisiae, GI6319699, Length=377, Percent_Identity=24.9336870026525, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24667955, Length=565, Percent_Identity=30.0884955752212, Blast_Score=216, Evalue=4e-56,
Organism=Drosophila melanogaster, GI62472339, Length=542, Percent_Identity=30.0738007380074, Blast_Score=204, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24667959, Length=498, Percent_Identity=29.718875502008, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI21355225, Length=566, Percent_Identity=25.7950530035336, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24581924, Length=529, Percent_Identity=26.4650283553875, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI18859661, Length=518, Percent_Identity=25.6756756756757, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21356947, Length=498, Percent_Identity=23.6947791164659, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648260, Length=498, Percent_Identity=24.4979919678715, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21355181, Length=531, Percent_Identity=22.9755178907721, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI20130357, Length=501, Percent_Identity=22.5548902195609, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI19922652, Length=508, Percent_Identity=23.6220472440945, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24656500, Length=499, Percent_Identity=23.2464929859719, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21356441, Length=503, Percent_Identity=22.0675944333996, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24653035, Length=501, Percent_Identity=21.5568862275449, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648253, Length=426, Percent_Identity=23.2394366197183, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648257, Length=495, Percent_Identity=23.4343434343434, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648255, Length=424, Percent_Identity=23.5849056603774, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI161076582, Length=512, Percent_Identity=21.2890625, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.1 [H]

Molecular weight: Translated: 60043; Mature: 60043

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDYTETFAGFSYQDTVSRQLHGSLDAMNACVECCDRHALPGRIALFWEGRDGTSETWTF
CCCHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHH
AQLKALSAKVAGFLKAQGVQPGDRVAGLLPRTPELLATILGTWRAGAVYQPLFTAFGPKA
HHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCHHH
IEHRLNASGAKFVVTDGANRSKLDDVADCPPIVTVAGAKGRGLVRGDFSFWAEVDRQPAE
HHHHCCCCCCEEEEECCCCCCCCCHHHCCCCEEEEECCCCCCEEECCHHHHHHHCCCCCC
FEPVMRRGDDPFLMMFTSGTTGPAKPLQVPLKAIVAFAGYMRDAVDLRDADAFWNLADPG
CHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
WAYGLYYAVTGPLALGHPTTFYDGPFSVESTCRVIEKYGITNLAGSPTAYRLLIAAGKAV
HHEEEEEEEECCEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHEEEEEECCCCC
SGPLRGKLRAVSSAGEPLNPEVIRWFANELGVTIHDHYGQTETGMVLCNHHALSHTVRMG
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCEEEECCHHHHHHHHHC
AAGFASPGHRVVVLDDENRELPAGQPGTLALDLTQSPMCWFPGYHGTPTRAFVGNYYLTG
CCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCEECCCCCCCCCCEEECCEEEEC
DSAELNDDGSISFIGRADDVITTSGYRVGPFDVESALIEHPAVVETAVIGKPDPERTELI
CCCCCCCCCCEEEEECCCCEEEECCEEECCCCHHHHHHCCCCHHEEEECCCCCCHHHHHH
KAFVVLDPQYRPTPELAEELRLHVRKRLAAHAYPREIEFVPELPKTPSGKVQRFILRNQE
EEHEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCHH
VAKAREAQGAQAA
HHHHHHCCCCCCC
>Mature Secondary Structure
MRDYTETFAGFSYQDTVSRQLHGSLDAMNACVECCDRHALPGRIALFWEGRDGTSETWTF
CCCHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHH
AQLKALSAKVAGFLKAQGVQPGDRVAGLLPRTPELLATILGTWRAGAVYQPLFTAFGPKA
HHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCHHH
IEHRLNASGAKFVVTDGANRSKLDDVADCPPIVTVAGAKGRGLVRGDFSFWAEVDRQPAE
HHHHCCCCCCEEEEECCCCCCCCCHHHCCCCEEEEECCCCCCEEECCHHHHHHHCCCCCC
FEPVMRRGDDPFLMMFTSGTTGPAKPLQVPLKAIVAFAGYMRDAVDLRDADAFWNLADPG
CHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
WAYGLYYAVTGPLALGHPTTFYDGPFSVESTCRVIEKYGITNLAGSPTAYRLLIAAGKAV
HHEEEEEEEECCEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHEEEEEECCCCC
SGPLRGKLRAVSSAGEPLNPEVIRWFANELGVTIHDHYGQTETGMVLCNHHALSHTVRMG
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCEEEECCHHHHHHHHHC
AAGFASPGHRVVVLDDENRELPAGQPGTLALDLTQSPMCWFPGYHGTPTRAFVGNYYLTG
CCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCEECCCCCCCCCCEEECCEEEEC
DSAELNDDGSISFIGRADDVITTSGYRVGPFDVESALIEHPAVVETAVIGKPDPERTELI
CCCCCCCCCCEEEEECCCCEEEECCEEECCCCHHHHHHCCCCHHEEEECCCCCCHHHHHH
KAFVVLDPQYRPTPELAEELRLHVRKRLAAHAYPREIEFVPELPKTPSGKVQRFILRNQE
EEHEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCHH
VAKAREAQGAQAA
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934817; 9387221; 9384377 [H]