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The map label for this gene is rpoE [H]

Identifier: 73538081

GI number: 73538081

Start: 908151

End: 908774

Strand: Reverse

Name: rpoE [H]

Synonym: Reut_B4251

Alternate gene names: 73538081

Gene position: 908774-908151 (Counterclockwise)

Preceding gene: 73538086

Following gene: 73538080

Centisome position: 33.34

GC content: 68.27

Gene sequence:

>624_bases
GTGAACGCCGCTGACGAGCGCCACCGGTTTGCCGAGCTGGTCCTGCCGCACCTCGATGCGGCCTACAACCTCGCACGATG
GCTGAGCGGCAGCGCGGCCGAAGCGGAAGACGTGGTGCAGGAGGCGCTGTTGCGCGCCTACACCTACTTTGGCAGCTATC
ACGGTGACAGGCCGCGGCCATGGCTGCTGGCCATCGTGCGCAATACGTGGTTCTCGGCTTGGAACCGCGACCAGCCGTCC
CGCGACGCTGCGACGTACGAAGACGGCGTCGATGCCGCCCAGGCCTTGCCCGGCTGGCAGGACGCCGCCGAACGGGAGCC
GCTGTGCTGCCTGCTGCGTGCAGAAGACGTTCGTCTCGTGCATGAAGCGCTCGCGCGCTTGTCCGCGCCGCTGCGGGAAG
TCATCGTGCTGCGCGAGCTGGAGGATATGTGTTACCGCGACATCGCTCAGGTTGCCGCGATTCCGCTCGGCACGGTCATG
TCACGCCTTGCGCGCGCACGGCAGGCACTGGCAGTGGCGGTAAGCGCACTGCGCGTGGAGCGGGCCGGACAGCCGTCGGG
CGGTGTGGCAACGGTCGATGGCCTGGCAGGAACGGCCGGGGAGGTGGGCAATGGACTGCAATGA

Upstream 100 bases:

>100_bases
AGGGCAATGCCGCGACGGCGGAATAAACTGGATCGAATCCCGGTAAACACGCTGAGGGGAGGCCCCAGCATGCTCCGTCC
GATTGACCCAAAGGAGTACC

Downstream 100 bases:

>100_bases
AGCACGGCTGCTGCTGCATGCCAGCGCGGACGGCGAACTCGGCCCCGGTGAGCTGGCGCGGCTGGGTCCGCACCTGGCGT
CCTGCCCGGTCTGCGCTGCA

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MNAADERHRFAELVLPHLDAAYNLARWLSGSAAEAEDVVQEALLRAYTYFGSYHGDRPRPWLLAIVRNTWFSAWNRDQPS
RDAATYEDGVDAAQALPGWQDAAEREPLCCLLRAEDVRLVHEALARLSAPLREVIVLRELEDMCYRDIAQVAAIPLGTVM
SRLARARQALAVAVSALRVERAGQPSGGVATVDGLAGTAGEVGNGLQ

Sequences:

>Translated_207_residues
MNAADERHRFAELVLPHLDAAYNLARWLSGSAAEAEDVVQEALLRAYTYFGSYHGDRPRPWLLAIVRNTWFSAWNRDQPS
RDAATYEDGVDAAQALPGWQDAAEREPLCCLLRAEDVRLVHEALARLSAPLREVIVLRELEDMCYRDIAQVAAIPLGTVM
SRLARARQALAVAVSALRVERAGQPSGGVATVDGLAGTAGEVGNGLQ
>Mature_207_residues
MNAADERHRFAELVLPHLDAAYNLARWLSGSAAEAEDVVQEALLRAYTYFGSYHGDRPRPWLLAIVRNTWFSAWNRDQPS
RDAATYEDGVDAAQALPGWQDAAEREPLCCLLRAEDVRLVHEALARLSAPLREVIVLRELEDMCYRDIAQVAAIPLGTVM
SRLARARQALAVAVSALRVERAGQPSGGVATVDGLAGTAGEVGNGLQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=150, Percent_Identity=34, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22590; Mature: 22590

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAADERHRFAELVLPHLDAAYNLARWLSGSAAEAEDVVQEALLRAYTYFGSYHGDRPRP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCH
WLLAIVRNTWFSAWNRDQPSRDAATYEDGVDAAQALPGWQDAAEREPLCCLLRAEDVRLV
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCEEEHHHHHHHHH
HEALARLSAPLREVIVLRELEDMCYRDIAQVAAIPLGTVMSRLARARQALAVAVSALRVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RAGQPSGGVATVDGLAGTAGEVGNGLQ
HCCCCCCCEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNAADERHRFAELVLPHLDAAYNLARWLSGSAAEAEDVVQEALLRAYTYFGSYHGDRPRP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCH
WLLAIVRNTWFSAWNRDQPSRDAATYEDGVDAAQALPGWQDAAEREPLCCLLRAEDVRLV
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCEEEHHHHHHHHH
HEALARLSAPLREVIVLRELEDMCYRDIAQVAAIPLGTVMSRLARARQALAVAVSALRVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RAGQPSGGVATVDGLAGTAGEVGNGLQ
HCCCCCCCEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]