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The map label for this gene is lcfB [H]

Identifier: 73537996

GI number: 73537996

Start: 808439

End: 810322

Strand: Direct

Name: lcfB [H]

Synonym: Reut_B4165

Alternate gene names: 73537996

Gene position: 808439-810322 (Clockwise)

Preceding gene: 73537995

Following gene: 73537997

Centisome position: 29.65

GC content: 69.43

Gene sequence:

>1884_bases
ATGACCGCATCCCTGGCCGGATCTGCCCCGGCCGTGCCGGCCCGCTACCGCCCGACTGTGTTCCCCGACCGCAAGACGGC
GGTGGAAACCCGTGCGGATGGCACCGTGCTGCTTCGCTGCGCTACGCCGCCCGCGGACCTGACCCAGCATGGCCTGCCCG
AGTTCCTGCCCCAGTGGGCCGAACGCCGCGGCGCCCTCCCCGCGTTCCGCAAACGTGATGCGGAAGGCGAATGGCGGTCG
CTGAGCTGGTCCGACCTGTGGCAGCAAGTGCAGGCAGTGGGCGCCGCGCTGCTTGAAATGGGCCTGGGCCAGGACCGCCC
GCTGATGCTGCTGTCCGGCAACTCCATCGAACAGGCGGTGCTGCTGCTTGCCGCTGAATATGTCGGCGTGCCGACCGCGC
CGGTATCGCCGGCCTACTCGCTGGTGAGCCGCGATTTCGCCCGCCTGCGCAGCGTGGCGGAATTGGTGCCGCCGGCGGCC
GTCTTCGTGCAGTCAGCCGCGCCATTCGATCGCGCCATTGCAGCACTGGACTACGGCGATGCCCCGGTCATCGCCGTGAG
CGGCGCTGCCGGCAGGCAGACGCCCTGGAGCCAGCTTGTTGCCACCGAACTGACGCCGGCACGCCTGCGCAGATTGGCCG
AGACGCACGCCGCGATCCGTCCGGAGCAGACCGGCCGCGTGCTGTTCACGTCTGGATCGACCGGTACGCCAAAAGGTGTG
GCGCTGTCGTACAGTAACCTGGCCGCTGTCGCCACGTACTTTGCGGACACCTATGCCGCGCTGACCGAACGCCAGCCGTC
ATTCCTGTGCTGGCTGCCGTGGCACCACGGCCTTGGCGGCGTGCTGAACCTGAGCCGCGCGGTCGTGCTGGGCGGCACGC
ACCACATCGATGATGGCAGGCCCCTGCCCGGCCAGTTCGAGCGCACCGTGCGCGACCTGCGCGAAGTCTCCCCGGCAATC
TTCACCAGCGTGCCGTCGGCGTGGACCATGCTGGCCACGGAACTGGAGCGTGACCCCGAACTGGCCCGCAGCCTGTTTGC
CGAGGCCGTGAATTTCTCCTACGGCGGCGCAAGCCTGCCGCGCGATGTGTGGGAGCGCATCCACCGCGCCGCGGAGGCCA
CCATTGGCGAGCGCATTGTGTTCTGCTCGGGCCTGGCCTGTACCGAGACCAGCGGCATGGGCATCTTCTGCACGCAGCCG
ACCGGCCAGGCGGGCAACGTCGGCGTGCCCATGCCCGGCTGCGAAGTCAAACTGATCCCGCTGGAAGGTGGCGACGGCCG
CTACGAAATCCGCATGCGCGGCCCGTTCGTGTTCGCCGGCTACGTCAAGCGTCCGGACCTGACCGCGGCCGCCTTCGACG
ACGAAGGCTACTTCTGCCTCGGCGACGCCGTACGACTGGCCGATCCGGCCGACCCCGCACGCGGCGTGACATTCGCCGGC
CGAGTGGTGGAGGACTTCAAGCTCACCAACGGCACATGGGTGCGCACCGGCGCGGTCCGCCTCGGCCTGGTCGAACAATG
CGCGCCGTTCATCAACGACGCCGTCATCTGTGGCCACGACCACGACTACCTCGCCGCGCTGGCCTGGCCCAACCTGGCCG
CCTGCCGCCGGCTCTCACCGGAACTGGCGGATATCGACGAGGAAACGCTGGTGCACCACCCGGTGGTCGTCAACGCGATC
CGCGAACGGCTGCGGGGCGGCAGCGCCGGCGCCAGCCTGGCCGTACGGCGTGTGCTGCTGATGGCCGAGCCGCCTTCCAT
CGACGCCAACGAAATTGCCGACAAGGGCTACGTCAACCAGGCCGCCACGCGCGCGCGCCGGGCCAACCTTGTCGATGAAC
TGTTCCAACCCCAGCCCGCGCCCCACGTGGCCTGCGCATCCTGA

Upstream 100 bases:

>100_bases
GTAAGTTAGCGCCATACCAACGCCACGCCCCGCGAGCAACGCAGGCGGCGGGCGCAAGACGTGCCACATCAGGCACGCCG
TACACCGTGGAGGCAACACC

Downstream 100 bases:

>100_bases
ACCCGCTTCCACCGGAGCCTTCCATGCAAATCACCCGTACGATCTACCGCGACGACCACGAAATGTTCCGCACCACGGTG
CGCCGCTTCCTCGAAAAGGA

Product: feruloyl-CoA synthase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 627; Mature: 626

Protein sequence:

>627_residues
MTASLAGSAPAVPARYRPTVFPDRKTAVETRADGTVLLRCATPPADLTQHGLPEFLPQWAERRGALPAFRKRDAEGEWRS
LSWSDLWQQVQAVGAALLEMGLGQDRPLMLLSGNSIEQAVLLLAAEYVGVPTAPVSPAYSLVSRDFARLRSVAELVPPAA
VFVQSAAPFDRAIAALDYGDAPVIAVSGAAGRQTPWSQLVATELTPARLRRLAETHAAIRPEQTGRVLFTSGSTGTPKGV
ALSYSNLAAVATYFADTYAALTERQPSFLCWLPWHHGLGGVLNLSRAVVLGGTHHIDDGRPLPGQFERTVRDLREVSPAI
FTSVPSAWTMLATELERDPELARSLFAEAVNFSYGGASLPRDVWERIHRAAEATIGERIVFCSGLACTETSGMGIFCTQP
TGQAGNVGVPMPGCEVKLIPLEGGDGRYEIRMRGPFVFAGYVKRPDLTAAAFDDEGYFCLGDAVRLADPADPARGVTFAG
RVVEDFKLTNGTWVRTGAVRLGLVEQCAPFINDAVICGHDHDYLAALAWPNLAACRRLSPELADIDEETLVHHPVVVNAI
RERLRGGSAGASLAVRRVLLMAEPPSIDANEIADKGYVNQAATRARRANLVDELFQPQPAPHVACAS

Sequences:

>Translated_627_residues
MTASLAGSAPAVPARYRPTVFPDRKTAVETRADGTVLLRCATPPADLTQHGLPEFLPQWAERRGALPAFRKRDAEGEWRS
LSWSDLWQQVQAVGAALLEMGLGQDRPLMLLSGNSIEQAVLLLAAEYVGVPTAPVSPAYSLVSRDFARLRSVAELVPPAA
VFVQSAAPFDRAIAALDYGDAPVIAVSGAAGRQTPWSQLVATELTPARLRRLAETHAAIRPEQTGRVLFTSGSTGTPKGV
ALSYSNLAAVATYFADTYAALTERQPSFLCWLPWHHGLGGVLNLSRAVVLGGTHHIDDGRPLPGQFERTVRDLREVSPAI
FTSVPSAWTMLATELERDPELARSLFAEAVNFSYGGASLPRDVWERIHRAAEATIGERIVFCSGLACTETSGMGIFCTQP
TGQAGNVGVPMPGCEVKLIPLEGGDGRYEIRMRGPFVFAGYVKRPDLTAAAFDDEGYFCLGDAVRLADPADPARGVTFAG
RVVEDFKLTNGTWVRTGAVRLGLVEQCAPFINDAVICGHDHDYLAALAWPNLAACRRLSPELADIDEETLVHHPVVVNAI
RERLRGGSAGASLAVRRVLLMAEPPSIDANEIADKGYVNQAATRARRANLVDELFQPQPAPHVACAS
>Mature_626_residues
TASLAGSAPAVPARYRPTVFPDRKTAVETRADGTVLLRCATPPADLTQHGLPEFLPQWAERRGALPAFRKRDAEGEWRSL
SWSDLWQQVQAVGAALLEMGLGQDRPLMLLSGNSIEQAVLLLAAEYVGVPTAPVSPAYSLVSRDFARLRSVAELVPPAAV
FVQSAAPFDRAIAALDYGDAPVIAVSGAAGRQTPWSQLVATELTPARLRRLAETHAAIRPEQTGRVLFTSGSTGTPKGVA
LSYSNLAAVATYFADTYAALTERQPSFLCWLPWHHGLGGVLNLSRAVVLGGTHHIDDGRPLPGQFERTVRDLREVSPAIF
TSVPSAWTMLATELERDPELARSLFAEAVNFSYGGASLPRDVWERIHRAAEATIGERIVFCSGLACTETSGMGIFCTQPT
GQAGNVGVPMPGCEVKLIPLEGGDGRYEIRMRGPFVFAGYVKRPDLTAAAFDDEGYFCLGDAVRLADPADPARGVTFAGR
VVEDFKLTNGTWVRTGAVRLGLVEQCAPFINDAVICGHDHDYLAALAWPNLAACRRLSPELADIDEETLVHHPVVVNAIR
ERLRGGSAGASLAVRRVLLMAEPPSIDANEIADKGYVNQAATRARRANLVDELFQPQPAPHVACAS

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42794754, Length=532, Percent_Identity=23.6842105263158, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI42794752, Length=532, Percent_Identity=23.6842105263158, Blast_Score=81, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556552, Length=320, Percent_Identity=25, Blast_Score=89, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 67349; Mature: 67218

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASLAGSAPAVPARYRPTVFPDRKTAVETRADGTVLLRCATPPADLTQHGLPEFLPQWA
CCCCCCCCCCCCCCCCCCCCCCCCCHHHEECCCCEEEEEECCCCHHHHHCCCHHHHHHHH
ERRGALPAFRKRDAEGEWRSLSWSDLWQQVQAVGAALLEMGLGQDRPLMLLSGNSIEQAV
HHCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHH
LLLAAEYVGVPTAPVSPAYSLVSRDFARLRSVAELVPPAAVFVQSAAPFDRAIAALDYGD
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHCCCCC
APVIAVSGAAGRQTPWSQLVATELTPARLRRLAETHAAIRPEQTGRVLFTSGSTGTPKGV
CCEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCE
ALSYSNLAAVATYFADTYAALTERQPSFLCWLPWHHGLGGVLNLSRAVVLGGTHHIDDGR
EEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCEEEECCCEECCCCC
PLPGQFERTVRDLREVSPAIFTSVPSAWTMLATELERDPELARSLFAEAVNFSYGGASLP
CCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCC
RDVWERIHRAAEATIGERIVFCSGLACTETSGMGIFCTQPTGQAGNVGVPMPGCEVKLIP
HHHHHHHHHHHHHHHCCEEEEECCCEEECCCCCEEEEECCCCCCCCCCCCCCCCCEEEEE
LEGGDGRYEIRMRGPFVFAGYVKRPDLTAAAFDDEGYFCLGDAVRLADPADPARGVTFAG
EECCCCEEEEEEECCEEEEEECCCCCCEEEEECCCCCEEECCCEECCCCCCCCCCCEEHH
RVVEDFKLTNGTWVRTGAVRLGLVEQCAPFINDAVICGHDHDYLAALAWPNLAACRRLSP
HHHHHEECCCCCEEEECHHHHHHHHHHHHHHCCCEEECCCCCCEEEECCCCHHHHHHCCC
ELADIDEETLVHHPVVVNAIRERLRGGSAGASLAVRRVLLMAEPPSIDANEIADKGYVNQ
HHHCCCHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCCCHHHHHCCCHHHH
AATRARRANLVDELFQPQPAPHVACAS
HHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TASLAGSAPAVPARYRPTVFPDRKTAVETRADGTVLLRCATPPADLTQHGLPEFLPQWA
CCCCCCCCCCCCCCCCCCCCCCCCHHHEECCCCEEEEEECCCCHHHHHCCCHHHHHHHH
ERRGALPAFRKRDAEGEWRSLSWSDLWQQVQAVGAALLEMGLGQDRPLMLLSGNSIEQAV
HHCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHH
LLLAAEYVGVPTAPVSPAYSLVSRDFARLRSVAELVPPAAVFVQSAAPFDRAIAALDYGD
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHCCCCC
APVIAVSGAAGRQTPWSQLVATELTPARLRRLAETHAAIRPEQTGRVLFTSGSTGTPKGV
CCEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCE
ALSYSNLAAVATYFADTYAALTERQPSFLCWLPWHHGLGGVLNLSRAVVLGGTHHIDDGR
EEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCEEEECCCEECCCCC
PLPGQFERTVRDLREVSPAIFTSVPSAWTMLATELERDPELARSLFAEAVNFSYGGASLP
CCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCC
RDVWERIHRAAEATIGERIVFCSGLACTETSGMGIFCTQPTGQAGNVGVPMPGCEVKLIP
HHHHHHHHHHHHHHHCCEEEEECCCEEECCCCCEEEEECCCCCCCCCCCCCCCCCEEEEE
LEGGDGRYEIRMRGPFVFAGYVKRPDLTAAAFDDEGYFCLGDAVRLADPADPARGVTFAG
EECCCCEEEEEEECCEEEEEECCCCCCEEEEECCCCCEEECCCEECCCCCCCCCCCEEHH
RVVEDFKLTNGTWVRTGAVRLGLVEQCAPFINDAVICGHDHDYLAALAWPNLAACRRLSP
HHHHHEECCCCCEEEECHHHHHHHHHHHHHHCCCEEECCCCCCEEEECCCCHHHHHHCCC
ELADIDEETLVHHPVVVNAIRERLRGGSAGASLAVRRVLLMAEPPSIDANEIADKGYVNQ
HHHCCCHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCCCHHHHHCCCHHHH
AATRARRANLVDELFQPQPAPHVACAS
HHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]