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The map label for this gene is 73537974

Identifier: 73537974

GI number: 73537974

Start: 782180

End: 783640

Strand: Reverse

Name: 73537974

Synonym: Reut_B4143

Alternate gene names: NA

Gene position: 783640-782180 (Counterclockwise)

Preceding gene: 73537976

Following gene: 73537973

Centisome position: 28.75

GC content: 69.13

Gene sequence:

>1461_bases
ATGAGCGCGATTCCCAGCATTCCGACCGATTCCATCATTGATCTCGACGCCGTTGCCCTGTCCCGGGCCATCCATGCGCG
CGAGGTGTCGTGCGTGGAGGTGCTTGATGCCTACCTGAGCCAGATCGACCGCCACAACCCGCGCGTCAATGCCATTGTGG
CGCCGATGGACCGGGATCCATTGCGAAAGCAGGCGGCGGGCCTCGATGACGAACTCGCCCGGGGGGCCAGCCGCGGTCCG
TTGCACGGTTTTCCGCAGGCGCCCAAAGACATCATGCCGGCCGCCGGCATGGTCACCACCAAGGGCTCGCCGATCTTCGC
CGGACAAGTCAGCCAGACCGACGCAGTGGTCTTCGAACGCATGCGCGCCGGCGGCGCGCTGTTCGTCGGCCGCACCAATT
CACCCGAGTTCGGCCTGGGCGGCCACACGTACAACCCGATCTACGGCACCACGCGCAATGCCTGGGATCCGGCACGCTCG
GCCGGCGGCAGCAGCGGCGGGGCGGCCGTAGCGGTGGCGCTGCGCATGCTTCCGGTGGCCGATGGCTCGGACATGATGGG
ATCGCTGCGGACGCCGGCCGCGTTCAACCATGTCTATGGGCTGCGCACCTCGTTCGGCTGCGTGCCGCATGGCCCGTCCG
AGGAAGTGTTCTTCCAGCAGTTCAGCGTAGCCGGCCCGATGGCGCGCAATGTGCCGGACCTGGCGTTGCTGCTGTCGGTT
CAGGCGGGCTTCGACGCGCGCTTGCCGCTGACGCGCCGCACCGAAGCGCCCGGCAGCTTTGCGCTGCCGCCCGAGCGCGA
CTGGGCTGGCGTGCGCATTGGCTGGCTCGGCGACCTCGGCGGCCACCTGCCGACCGAACCCGGCCTGCTTGATACATGCG
TCATGGCGCTCAGCCATCTACGGGCGATCGGCTGCACAGTCGATGCCGCACTGCCGGATTTCAACCTCGAACGCCTGTGG
CACGCATGGATCGACCTGCGCAGCTTTGCTGTGGCCGGCGCCAACGGCGCGCTGTACCGCGATCCCGCGAAGCGCGCGTT
GCTCAAGCCGGAAGCCGTCTGGGAAATCGAGCGCGGGCTGGCGCTGTCAGGCTCCCGCGTGTACGAGGCCATGGCGATAC
GTAGCACGTGGTACCAGACACTGCGCACGCTGTTCGAGCGCTTCGACTACCTCGTCATGCCGGCCGCGCAGGTGTTCCCG
TTCGACGCCGATATGGACTGGCCCCATGCGATCGACGGGCGCGACATGGACACGTATCACCGCTGGATGCAGGCCGTGGT
GCCCGCGACCATGGCCGGCCTGCCCGCGCTGGCCGCGCCCGCGGGCTTTGGCCGCGGCGGGCTGCCGGCCGGCATCCAGA
TCATCGGCCCGGCGCAGGCGGACGCGGAGGTGCTGCAGCTTGGCCACGCCTATGACCAGGCGGGCGGCTTCTCGCGCGTG
CGCAGCCCCTGGCTGGCGTAG

Upstream 100 bases:

>100_bases
CGTTTGCGTTTTACGCAAGGGTATTTGAAAAATTCCTCGATTGTAGGAAAACCACGCCGCCCCTATGCTCTTTCCTGACC
AGACAGGAGACAGGCTTCCC

Downstream 100 bases:

>100_bases
GTGCCCGACCCGACCGACACATCGAGTTTGTCCACGGGCAGCAAGCTGCTGGCCGTGTTGACCGCATGGCTTCGTCTTTC
CCCAAACAGCAGGCAGGAGT

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSAIPSIPTDSIIDLDAVALSRAIHAREVSCVEVLDAYLSQIDRHNPRVNAIVAPMDRDPLRKQAAGLDDELARGASRGP
LHGFPQAPKDIMPAAGMVTTKGSPIFAGQVSQTDAVVFERMRAGGALFVGRTNSPEFGLGGHTYNPIYGTTRNAWDPARS
AGGSSGGAAVAVALRMLPVADGSDMMGSLRTPAAFNHVYGLRTSFGCVPHGPSEEVFFQQFSVAGPMARNVPDLALLLSV
QAGFDARLPLTRRTEAPGSFALPPERDWAGVRIGWLGDLGGHLPTEPGLLDTCVMALSHLRAIGCTVDAALPDFNLERLW
HAWIDLRSFAVAGANGALYRDPAKRALLKPEAVWEIERGLALSGSRVYEAMAIRSTWYQTLRTLFERFDYLVMPAAQVFP
FDADMDWPHAIDGRDMDTYHRWMQAVVPATMAGLPALAAPAGFGRGGLPAGIQIIGPAQADAEVLQLGHAYDQAGGFSRV
RSPWLA

Sequences:

>Translated_486_residues
MSAIPSIPTDSIIDLDAVALSRAIHAREVSCVEVLDAYLSQIDRHNPRVNAIVAPMDRDPLRKQAAGLDDELARGASRGP
LHGFPQAPKDIMPAAGMVTTKGSPIFAGQVSQTDAVVFERMRAGGALFVGRTNSPEFGLGGHTYNPIYGTTRNAWDPARS
AGGSSGGAAVAVALRMLPVADGSDMMGSLRTPAAFNHVYGLRTSFGCVPHGPSEEVFFQQFSVAGPMARNVPDLALLLSV
QAGFDARLPLTRRTEAPGSFALPPERDWAGVRIGWLGDLGGHLPTEPGLLDTCVMALSHLRAIGCTVDAALPDFNLERLW
HAWIDLRSFAVAGANGALYRDPAKRALLKPEAVWEIERGLALSGSRVYEAMAIRSTWYQTLRTLFERFDYLVMPAAQVFP
FDADMDWPHAIDGRDMDTYHRWMQAVVPATMAGLPALAAPAGFGRGGLPAGIQIIGPAQADAEVLQLGHAYDQAGGFSRV
RSPWLA
>Mature_485_residues
SAIPSIPTDSIIDLDAVALSRAIHAREVSCVEVLDAYLSQIDRHNPRVNAIVAPMDRDPLRKQAAGLDDELARGASRGPL
HGFPQAPKDIMPAAGMVTTKGSPIFAGQVSQTDAVVFERMRAGGALFVGRTNSPEFGLGGHTYNPIYGTTRNAWDPARSA
GGSSGGAAVAVALRMLPVADGSDMMGSLRTPAAFNHVYGLRTSFGCVPHGPSEEVFFQQFSVAGPMARNVPDLALLLSVQ
AGFDARLPLTRRTEAPGSFALPPERDWAGVRIGWLGDLGGHLPTEPGLLDTCVMALSHLRAIGCTVDAALPDFNLERLWH
AWIDLRSFAVAGANGALYRDPAKRALLKPEAVWEIERGLALSGSRVYEAMAIRSTWYQTLRTLFERFDYLVMPAAQVFPF
DADMDWPHAIDGRDMDTYHRWMQAVVPATMAGLPALAAPAGFGRGGLPAGIQIIGPAQADAEVLQLGHAYDQAGGFSRVR
SPWLA

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=516, Percent_Identity=25.3875968992248, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI222831590, Length=506, Percent_Identity=26.4822134387352, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI166795287, Length=213, Percent_Identity=27.2300469483568, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17537465, Length=253, Percent_Identity=30.4347826086957, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17556264, Length=192, Percent_Identity=32.8125, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=447, Percent_Identity=25.9507829977629, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=316, Percent_Identity=27.5316455696203, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=275, Percent_Identity=31.2727272727273, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556278, Length=275, Percent_Identity=31.2727272727273, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=220, Percent_Identity=28.1818181818182, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538254, Length=195, Percent_Identity=26.1538461538462, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=410, Percent_Identity=29.5121951219512, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6320448, Length=228, Percent_Identity=28.9473684210526, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648435, Length=465, Percent_Identity=24.0860215053763, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24648437, Length=465, Percent_Identity=24.0860215053763, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI45550774, Length=485, Percent_Identity=23.7113402061856, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24652985, Length=260, Percent_Identity=32.3076923076923, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19922090, Length=260, Percent_Identity=32.3076923076923, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24652981, Length=260, Percent_Identity=32.3076923076923, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24652983, Length=260, Percent_Identity=32.3076923076923, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24644968, Length=504, Percent_Identity=25.3968253968254, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21356731, Length=268, Percent_Identity=29.1044776119403, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI161078093, Length=519, Percent_Identity=23.5067437379576, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24648113, Length=431, Percent_Identity=28.538283062645, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648441, Length=390, Percent_Identity=23.5897435897436, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648439, Length=390, Percent_Identity=23.5897435897436, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 52068; Mature: 51937

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIPSIPTDSIIDLDAVALSRAIHAREVSCVEVLDAYLSQIDRHNPRVNAIVAPMDRDP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCH
LRKQAAGLDDELARGASRGPLHGFPQAPKDIMPAAGMVTTKGSPIFAGQVSQTDAVVFER
HHHHHCCCCHHHHCCCCCCCCCCCCCCCHHHCCCCCEEEECCCEEEECCCCCHHHHHHHH
MRAGGALFVGRTNSPEFGLGGHTYNPIYGTTRNAWDPARSAGGSSGGAAVAVALRMLPVA
HHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCC
DGSDMMGSLRTPAAFNHVYGLRTSFGCVPHGPSEEVFFQQFSVAGPMARNVPDLALLLSV
CCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHCCCCCEEEEEEH
QAGFDARLPLTRRTEAPGSFALPPERDWAGVRIGWLGDLGGHLPTEPGLLDTCVMALSHL
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
RAIGCTVDAALPDFNLERLWHAWIDLRSFAVAGANGALYRDPAKRALLKPEAVWEIERGL
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCEECCHHHHHHCCCHHHHHHHCCC
ALSGSRVYEAMAIRSTWYQTLRTLFERFDYLVMPAAQVFPFDADMDWPHAIDGRDMDTYH
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHCCCCCCCCCCCCCCCCCHHHHH
RWMQAVVPATMAGLPALAAPAGFGRGGLPAGIQIIGPAQADAEVLQLGHAYDQAGGFSRV
HHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCHHHH
RSPWLA
CCCCCC
>Mature Secondary Structure 
SAIPSIPTDSIIDLDAVALSRAIHAREVSCVEVLDAYLSQIDRHNPRVNAIVAPMDRDP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCH
LRKQAAGLDDELARGASRGPLHGFPQAPKDIMPAAGMVTTKGSPIFAGQVSQTDAVVFER
HHHHHCCCCHHHHCCCCCCCCCCCCCCCHHHCCCCCEEEECCCEEEECCCCCHHHHHHHH
MRAGGALFVGRTNSPEFGLGGHTYNPIYGTTRNAWDPARSAGGSSGGAAVAVALRMLPVA
HHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCC
DGSDMMGSLRTPAAFNHVYGLRTSFGCVPHGPSEEVFFQQFSVAGPMARNVPDLALLLSV
CCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHCCCCCEEEEEEH
QAGFDARLPLTRRTEAPGSFALPPERDWAGVRIGWLGDLGGHLPTEPGLLDTCVMALSHL
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
RAIGCTVDAALPDFNLERLWHAWIDLRSFAVAGANGALYRDPAKRALLKPEAVWEIERGL
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCEECCHHHHHHCCCHHHHHHHCCC
ALSGSRVYEAMAIRSTWYQTLRTLFERFDYLVMPAAQVFPFDADMDWPHAIDGRDMDTYH
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHCCCCCCCCCCCCCCCCCHHHHH
RWMQAVVPATMAGLPALAAPAGFGRGGLPAGIQIIGPAQADAEVLQLGHAYDQAGGFSRV
HHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCHHHH
RSPWLA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]