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The map label for this gene is oprM [H]

Identifier: 73537921

GI number: 73537921

Start: 728884

End: 730353

Strand: Reverse

Name: oprM [H]

Synonym: Reut_B4088

Alternate gene names: 73537921

Gene position: 730353-728884 (Counterclockwise)

Preceding gene: 73537922

Following gene: 73537920

Centisome position: 26.79

GC content: 67.48

Gene sequence:

>1470_bases
ATGAAGAAGACTTTGACCTGTGCTGCCGCCGTCGCCCTGCTGGCCGGCTGCAGCTTGATTCCCTCCTATGAACAGCCGTC
GCTGCCAGTGGCGCAAGCCTACCCGCAAGGCGCCGCGTATCCGACTGCCGATGCCGCCGCCGGCGCACAGCAGATGCGTG
CGACCGATCTTGGCTGGCGCGATTTCTTTGCCGACGCGCGGCTGCGCCGCCTGATCGAAGTCGCACTCGACAACAACCGC
GACCTGCGCGTGGCGGCACTGAACGTCGAAGCATTCCGTGCGCAATACCAGATCGAACGGGCCGACCTGTTCCCCGCGAT
CGGCGTGGCCGCGCAGGAAACGCGCCAGCGCATTCCCGCGGACCTGTCCACCACCGGGCGGCGCATCATCGCGAGCCAGT
ATGGCGTGTCGCTCGGCACGACCGCATGGGAGCTTGACCTGTTCGGCCGTATCCGCAGCCTCAGCGAAGCGGCACTGGAG
CAATATTTCGCAAGCGACGAAACCCGGCGCAGTGCTCAGATCGCGCTGGTGGCCAGCGTGGCCAACGCCTACCTGGCACT
GCTCGCCGACGACGCGCAGCTCACGCTCACGCGCAGTACGCTGGCGAACTACGAACGCAGCTATGGCCTGACGCGCCGCA
GCTACGAGGAAGGCATTGCCTCGCGTCTGGACCTGCGGCAGTCGCAGACCGCCGTGGAAAACGCGCGTGCCAGCCTGGCG
CGCTACACGCGCCTGGTCGCGCAGGACGAGAATGCGCTGGTGCTGTTGCTGGGCACCGGCATGCCGGACGACCTGCCGGC
GCCGTTGCCGCTCGACCAGAAGCTGACGGCGGACGTGCCTGCCGGATTGCCATCCGACCTGCTGCAGAACCGTCCCGACG
TGCTTGCAGCCGAACACCGCCTGCGCTCGGCCAACGCCAATATCGGCGCGGCACGCGCGGCGTTTTTCCCGCGCATCGCC
CTGACGGCTTCGGCGGGCACGGCCAGCAATGAACTGTCCGGCCTGTTCAAGGCCGGATCGGGCACCTGGCTGTTCCAGCC
GCAGATCAGCCTGCCGCTGTTCACGGCCGGCAGCCTGCGCGCGAGCCTCGACTACGCAAAGATCCAGAAGGACATCAACG
TCGCGCAGTACGAGCGCACGATCCAGACAGCCTTCCGCGAAGTTTCCGACGGCCTCGCCGCGCGCGGCACGTATACGAGC
CAACTCGAAGCGCAGGTGCGTTTTGTCGAAGCGGCGCAGGATGCCTACAACCTCGCAGACCGTCGCTACCGCACCGGCGT
GGACAGCTACCTGGCGGTGCTGGATGCGCAGCGCTCGCTGTACAACGCCCAGCAGCTCCTGATCGTCGCACGACAGGAAC
AGCTGACCAGCGAGGTCAATCTCTACAAGGCCCTCGGCGGCGGCTGGCATGACCAGACCGGCCCTGCTGCCGACACCGGC
GGCCAGCCGGCGCCCGCGCCCGCGTCCTGA

Upstream 100 bases:

>100_bases
TCTTCTGGGTGCCGTTGTTCTTTGTCGCCGTCAATTCGCTGTTCGCACGCAAACGCAAGCCGAGCCAGCCTGACACCCCC
GCGGAAAAAGGCGCGCTGCA

Downstream 100 bases:

>100_bases
TCCCCGGCGGGGCCAGCCGGCGCAAAGTTGGCTGGCCATTGTTGCGCGGGTCCGGCGGGACCTTCATGGCTCCTACAATG
ACGAGGTCGCATGTCATTCC

Product: RND efflux system outer membrane lipoprotein NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MKKTLTCAAAVALLAGCSLIPSYEQPSLPVAQAYPQGAAYPTADAAAGAQQMRATDLGWRDFFADARLRRLIEVALDNNR
DLRVAALNVEAFRAQYQIERADLFPAIGVAAQETRQRIPADLSTTGRRIIASQYGVSLGTTAWELDLFGRIRSLSEAALE
QYFASDETRRSAQIALVASVANAYLALLADDAQLTLTRSTLANYERSYGLTRRSYEEGIASRLDLRQSQTAVENARASLA
RYTRLVAQDENALVLLLGTGMPDDLPAPLPLDQKLTADVPAGLPSDLLQNRPDVLAAEHRLRSANANIGAARAAFFPRIA
LTASAGTASNELSGLFKAGSGTWLFQPQISLPLFTAGSLRASLDYAKIQKDINVAQYERTIQTAFREVSDGLAARGTYTS
QLEAQVRFVEAAQDAYNLADRRYRTGVDSYLAVLDAQRSLYNAQQLLIVARQEQLTSEVNLYKALGGGWHDQTGPAADTG
GQPAPAPAS

Sequences:

>Translated_489_residues
MKKTLTCAAAVALLAGCSLIPSYEQPSLPVAQAYPQGAAYPTADAAAGAQQMRATDLGWRDFFADARLRRLIEVALDNNR
DLRVAALNVEAFRAQYQIERADLFPAIGVAAQETRQRIPADLSTTGRRIIASQYGVSLGTTAWELDLFGRIRSLSEAALE
QYFASDETRRSAQIALVASVANAYLALLADDAQLTLTRSTLANYERSYGLTRRSYEEGIASRLDLRQSQTAVENARASLA
RYTRLVAQDENALVLLLGTGMPDDLPAPLPLDQKLTADVPAGLPSDLLQNRPDVLAAEHRLRSANANIGAARAAFFPRIA
LTASAGTASNELSGLFKAGSGTWLFQPQISLPLFTAGSLRASLDYAKIQKDINVAQYERTIQTAFREVSDGLAARGTYTS
QLEAQVRFVEAAQDAYNLADRRYRTGVDSYLAVLDAQRSLYNAQQLLIVARQEQLTSEVNLYKALGGGWHDQTGPAADTG
GQPAPAPAS
>Mature_489_residues
MKKTLTCAAAVALLAGCSLIPSYEQPSLPVAQAYPQGAAYPTADAAAGAQQMRATDLGWRDFFADARLRRLIEVALDNNR
DLRVAALNVEAFRAQYQIERADLFPAIGVAAQETRQRIPADLSTTGRRIIASQYGVSLGTTAWELDLFGRIRSLSEAALE
QYFASDETRRSAQIALVASVANAYLALLADDAQLTLTRSTLANYERSYGLTRRSYEEGIASRLDLRQSQTAVENARASLA
RYTRLVAQDENALVLLLGTGMPDDLPAPLPLDQKLTADVPAGLPSDLLQNRPDVLAAEHRLRSANANIGAARAAFFPRIA
LTASAGTASNELSGLFKAGSGTWLFQPQISLPLFTAGSLRASLDYAKIQKDINVAQYERTIQTAFREVSDGLAARGTYTS
QLEAQVRFVEAAQDAYNLADRRYRTGVDSYLAVLDAQRSLYNAQQLLIVARQEQLTSEVNLYKALGGGWHDQTGPAADTG
GQPAPAPAS

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=475, Percent_Identity=40, Blast_Score=315, Evalue=5e-87,
Organism=Escherichia coli, GI2367348, Length=501, Percent_Identity=24.9500998003992, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52817; Mature: 52817

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTLTCAAAVALLAGCSLIPSYEQPSLPVAQAYPQGAAYPTADAAAGAQQMRATDLGWR
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHH
DFFADARLRRLIEVALDNNRDLRVAALNVEAFRAQYQIERADLFPAIGVAAQETRQRIPA
HHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
DLSTTGRRIIASQYGVSLGTTAWELDLFGRIRSLSEAALEQYFASDETRRSAQIALVASV
CCHHCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ANAYLALLADDAQLTLTRSTLANYERSYGLTRRSYEEGIASRLDLRQSQTAVENARASLA
HHHHHHHEECCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYTRLVAQDENALVLLLGTGMPDDLPAPLPLDQKLTADVPAGLPSDLLQNRPDVLAAEHR
HHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHH
LRSANANIGAARAAFFPRIALTASAGTASNELSGLFKAGSGTWLFQPQISLPLFTAGSLR
HHHCCCCCCHHHHHHCCCEEEEECCCCCCHHHHHHHCCCCCCEEECCCCCCCEEECCCHH
ASLDYAKIQKDINVAQYERTIQTAFREVSDGLAARGTYTSQLEAQVRFVEAAQDAYNLAD
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
RRYRTGVDSYLAVLDAQRSLYNAQQLLIVARQEQLTSEVNLYKALGGGWHDQTGPAADTG
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GQPAPAPAS
CCCCCCCCC
>Mature Secondary Structure
MKKTLTCAAAVALLAGCSLIPSYEQPSLPVAQAYPQGAAYPTADAAAGAQQMRATDLGWR
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHH
DFFADARLRRLIEVALDNNRDLRVAALNVEAFRAQYQIERADLFPAIGVAAQETRQRIPA
HHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
DLSTTGRRIIASQYGVSLGTTAWELDLFGRIRSLSEAALEQYFASDETRRSAQIALVASV
CCHHCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ANAYLALLADDAQLTLTRSTLANYERSYGLTRRSYEEGIASRLDLRQSQTAVENARASLA
HHHHHHHEECCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYTRLVAQDENALVLLLGTGMPDDLPAPLPLDQKLTADVPAGLPSDLLQNRPDVLAAEHR
HHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHH
LRSANANIGAARAAFFPRIALTASAGTASNELSGLFKAGSGTWLFQPQISLPLFTAGSLR
HHHCCCCCCHHHHHHCCCEEEEECCCCCCHHHHHHHCCCCCCEEECCCCCCCEEECCCHH
ASLDYAKIQKDINVAQYERTIQTAFREVSDGLAARGTYTSQLEAQVRFVEAAQDAYNLAD
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
RRYRTGVDSYLAVLDAQRSLYNAQQLLIVARQEQLTSEVNLYKALGGGWHDQTGPAADTG
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GQPAPAPAS
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]