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The map label for this gene is nemA [H]

Identifier: 73537897

GI number: 73537897

Start: 703138

End: 704268

Strand: Reverse

Name: nemA [H]

Synonym: Reut_B4064

Alternate gene names: 73537897

Gene position: 704268-703138 (Counterclockwise)

Preceding gene: 73537898

Following gene: 73537894

Centisome position: 25.83

GC content: 66.76

Gene sequence:

>1131_bases
ATGGCGCTTGCCGTCGTGCCAAAGCACTGCTGTGCGCTTCTCCCACCCATACGAAAATACGCAAGGCCAGACCTCATGCC
TACCCTCTTCGACCCCATCCGCATCGGCGACCTCGACCTGCCCAACCGCGTCATCATGGCGCCGCTGACCCGCTCGCGCG
CCGTCGGCGGCAAGCGTGTGCCGAACGCACTGATGGCCGAGTATTACGTGCAACGCGCGTCCGCCGGCCTCATCATCTCG
GAAGCCACGGCGGTGACGCCGCAGGGCGTCGGCTATGCCGACACGCCGGGCATCTGGACCGACGAGCAAGTCGCGGGCTG
GAAACACGTGACCGACGCAGTCCATGCCGCTGGCGGCCGCATCTTCATGCAGCTCTGGCACGTCGGCCGGATCTCGGACC
CGATCTTCCTCGACGGCGAACTGCCTGTGGCGCCGAGCGCGATCGCGGCGCAAGGCCACGTGAGCCTGGTGCGCCCGAAG
CGTGCCTACGTCACGCCCCGTGCGCTGGAAACCGAGGAAATCGCTGGCGTGATCGCGGCCTACCGGCACGGTGCCGAAAA
TGCCAAAAAGGCCGGCTTCGACGGCGTTGAAATCCACGGTGCCAACGGCTACCTGCTCGACCAGTTCCTGCAGGATTCGA
CCAACCAGCGCACGGATGCCTATGGTGGCCCCATCGAAAACCGCGCGCGGCTGCTGCTCGAAATTACCGACGCATGCATC
GACGTGTGGGGTGCCGACCGCGTCGGCGTGCACCTGTCGCCGCGCGGTGACGTGCACTCGATGGGCGACTCCGATCTGGC
GGCGACGTTCGGCTACGTGGCGCGCGAACTGGGCAAGCGCCGCATTGCCTTCATCTGCGCGCGCGAGGCACTCGGCGACA
ATCGCCTGGGTCCGGCCCTGAAGGCGGCTTTCGGCGGCGTCTATATCGCCAACGAGAAAATGACCAAGCAGACCGCCGAA
CACGTGCTGGCCGCCGGCGAAGCCGACGCCGTCGCATTCGGGCAGCTCTTCATCGCCAACCCGGACTTGCCGCGCCGCCT
TGCGCTCGACGCATCGCTCAACGAGCCCCGGCCGGAGACGTTCTACCACCCGGGTGCCGAGGGATATACCGACTATCCGG
CGCTGGTGTGA

Upstream 100 bases:

>100_bases
CGCGCGGCGCATCGGGCAATGGGTCTTCTTCAAGCGCGACGAAGAGACCATTCAGGCGTTCCTCGACCATATTCACCAGG
ACCTTTGAGTTCGTACCGGC

Downstream 100 bases:

>100_bases
CGAGCGGCAAGAACGGCCGTTAGACCCGGTTCGCACGGGCAAGGGCTACCGCTCTTGCCCGGTTTCCGTAGACCCCATCG
CCGGGGTAGGCCCTAGAATT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MALAVVPKHCCALLPPIRKYARPDLMPTLFDPIRIGDLDLPNRVIMAPLTRSRAVGGKRVPNALMAEYYVQRASAGLIIS
EATAVTPQGVGYADTPGIWTDEQVAGWKHVTDAVHAAGGRIFMQLWHVGRISDPIFLDGELPVAPSAIAAQGHVSLVRPK
RAYVTPRALETEEIAGVIAAYRHGAENAKKAGFDGVEIHGANGYLLDQFLQDSTNQRTDAYGGPIENRARLLLEITDACI
DVWGADRVGVHLSPRGDVHSMGDSDLAATFGYVARELGKRRIAFICAREALGDNRLGPALKAAFGGVYIANEKMTKQTAE
HVLAAGEADAVAFGQLFIANPDLPRRLALDASLNEPRPETFYHPGAEGYTDYPALV

Sequences:

>Translated_376_residues
MALAVVPKHCCALLPPIRKYARPDLMPTLFDPIRIGDLDLPNRVIMAPLTRSRAVGGKRVPNALMAEYYVQRASAGLIIS
EATAVTPQGVGYADTPGIWTDEQVAGWKHVTDAVHAAGGRIFMQLWHVGRISDPIFLDGELPVAPSAIAAQGHVSLVRPK
RAYVTPRALETEEIAGVIAAYRHGAENAKKAGFDGVEIHGANGYLLDQFLQDSTNQRTDAYGGPIENRARLLLEITDACI
DVWGADRVGVHLSPRGDVHSMGDSDLAATFGYVARELGKRRIAFICAREALGDNRLGPALKAAFGGVYIANEKMTKQTAE
HVLAAGEADAVAFGQLFIANPDLPRRLALDASLNEPRPETFYHPGAEGYTDYPALV
>Mature_375_residues
ALAVVPKHCCALLPPIRKYARPDLMPTLFDPIRIGDLDLPNRVIMAPLTRSRAVGGKRVPNALMAEYYVQRASAGLIISE
ATAVTPQGVGYADTPGIWTDEQVAGWKHVTDAVHAAGGRIFMQLWHVGRISDPIFLDGELPVAPSAIAAQGHVSLVRPKR
AYVTPRALETEEIAGVIAAYRHGAENAKKAGFDGVEIHGANGYLLDQFLQDSTNQRTDAYGGPIENRARLLLEITDACID
VWGADRVGVHLSPRGDVHSMGDSDLAATFGYVARELGKRRIAFICAREALGDNRLGPALKAAFGGVYIANEKMTKQTAEH
VLAAGEADAVAFGQLFIANPDLPRRLALDASLNEPRPETFYHPGAEGYTDYPALV

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=360, Percent_Identity=47.7777777777778, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI1789463, Length=335, Percent_Identity=31.3432835820896, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17564188, Length=309, Percent_Identity=26.8608414239482, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=170, Percent_Identity=36.4705882352941, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17565138, Length=348, Percent_Identity=26.7241379310345, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=299, Percent_Identity=27.7591973244147, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17559802, Length=248, Percent_Identity=30.6451612903226, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=167, Percent_Identity=34.1317365269461, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325086, Length=381, Percent_Identity=38.8451443569554, Blast_Score=224, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6321973, Length=381, Percent_Identity=36.2204724409449, Blast_Score=211, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40519; Mature: 40387

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAVVPKHCCALLPPIRKYARPDLMPTLFDPIRIGDLDLPNRVIMAPLTRSRAVGGKRV
CCEEECHHHHHHHCHHHHHHCCCCCCHHHHCCEEECCCCCCCCEEECCCHHHHCCCCCCC
PNALMAEYYVQRASAGLIISEATAVTPQGVGYADTPGIWTDEQVAGWKHVTDAVHAAGGR
CHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCH
IFMQLWHVGRISDPIFLDGELPVAPSAIAAQGHVSLVRPKRAYVTPRALETEEIAGVIAA
HHHHHHHHCCCCCCEEECCCCCCCCHHHHCCCCEEEECCCHHCCCCCCCCHHHHHHHHHH
YRHGAENAKKAGFDGVEIHGANGYLLDQFLQDSTNQRTDAYGGPIENRARLLLEITDACI
HHHCCCHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
DVWGADRVGVHLSPRGDVHSMGDSDLAATFGYVARELGKRRIAFICAREALGDNRLGPAL
HHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCHHEEHEEEHHHCCCCCCCHHH
KAAFGGVYIANEKMTKQTAEHVLAAGEADAVAFGQLFIANPDLPRRLALDASLNEPRPET
HHHHCCEEEECCHHHHHHHHHHHHCCCCCCEEECEEEEECCCCCCEEEECCCCCCCCCCC
FYHPGAEGYTDYPALV
CCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ALAVVPKHCCALLPPIRKYARPDLMPTLFDPIRIGDLDLPNRVIMAPLTRSRAVGGKRV
CEEECHHHHHHHCHHHHHHCCCCCCHHHHCCEEECCCCCCCCEEECCCHHHHCCCCCCC
PNALMAEYYVQRASAGLIISEATAVTPQGVGYADTPGIWTDEQVAGWKHVTDAVHAAGGR
CHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCH
IFMQLWHVGRISDPIFLDGELPVAPSAIAAQGHVSLVRPKRAYVTPRALETEEIAGVIAA
HHHHHHHHCCCCCCEEECCCCCCCCHHHHCCCCEEEECCCHHCCCCCCCCHHHHHHHHHH
YRHGAENAKKAGFDGVEIHGANGYLLDQFLQDSTNQRTDAYGGPIENRARLLLEITDACI
HHHCCCHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
DVWGADRVGVHLSPRGDVHSMGDSDLAATFGYVARELGKRRIAFICAREALGDNRLGPAL
HHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCHHEEHEEEHHHCCCCCCCHHH
KAAFGGVYIANEKMTKQTAEHVLAAGEADAVAFGQLFIANPDLPRRLALDASLNEPRPET
HHHHCCEEEECCHHHHHHHHHHHHCCCCCCEEECEEEEECCCCCCEEEECCCCCCCCCCC
FYHPGAEGYTDYPALV
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]