Click here to switch to the map view.

The map label for this gene is ydgK [H]

Identifier: 73537869

GI number: 73537869

Start: 678720

End: 679940

Strand: Direct

Name: ydgK [H]

Synonym: Reut_B4036

Alternate gene names: 73537869

Gene position: 678720-679940 (Clockwise)

Preceding gene: 73537867

Following gene: 73537872

Centisome position: 24.9

GC content: 67.08

Gene sequence:

>1221_bases
ATGCCCCAATCCTCACGCAGCCAGAAGGCTGCGCGCTTCCCCGCCTGGCTGCTGATCTGCGCCTCGCTGACGGCCATTGC
GCCGCTGTCGATCGACATGTACCTGCCCAGCTTTCCCGCGTTGGCCGCGGATATGCGCGTCGATATCGGCCAGATTCAGT
TGACGCTGGGCACCTTTCTGGTCGGCCTGGCCCTGGGGCAGGCCTTCTACGGCCCGATCAGCGACCGTTTCGGACGCAAG
CGCCCGCTGTACGTTGGCCTTGGCCTGTACGCACTGGCTGCGGCCGGCTGCGCCATGGCGCGCAGCGCCGAGTCCCTGAT
GCTCTGGCGATTTCTTCAGGCGCTGGGGGGCTGCGCAGGCATGGTCATGGCCCGCGCCGTCATCCGCGACCGGCTCGACG
CCCATGAGTCGGCCCGGGCATTCTCGTCGATGATGCTGGTGATGGGCCTGGCCCCGATCCTGGCGCCGATCATCGGCGGC
GCCATTCTCAGTGCATCGGGCTGGCGCGCGATCTTCTGGTTGTTGACGTTGGCGGGGCTGCTGATCCTGGCGCTGGTCCA
CTTCCGGATGGAAGAATCCCTGCCGCGCGAACGTGCCGCACCGCTGCATCCCGGCGCCATCGTGGCAAGCTACACAGGAT
TGCTGCGGGACCGTGAATTCCTTGGCTATTCGCTGTCAGCCGGCTTCGGGCAGGCCGGCATGTTCGCGTATATCTCGGGT
TCGCCGTTCGTGCTGATCGAACTCAACGGCATCGATCCCTCGCACTATGGCTTTGTGTTCGGAGCCAATGCGCTCGGGCT
GATCGGCATGTCGCAACTCAATGCGCGCCTGGTCCACGGCCGCACGCTGGATAGCGTGCTCCGACGCGCGGTGCTCGCGC
CATGCGCCGCAGGCCTGATACTGGCCGCTGCGGCGCTGGCAGGCGTGCTTGTGCTGCCGGTGCTGCTGGCCGGATTCTTT
GTCTTTATCGGCTCGCTGGGCTGTATCACACCGAATGCGTCTGCACTGGCGCTGACGCATCAGGGGCATCGTGCCGGGAC
CGCATCGGCACTGATGGGCACGCTGCAGTTTTCGCTTGGTACGGTGGCCGGCGCCGCCGTCAGCTTGTGGCACGATGGCA
CGGCCCTGCCGCTCGCGGTCGTGATGGCCGCGTGCGGCATGGGCGCGGTGGCGTTGCGCGCGTGGGCTTGCGCCGGATCG
TCGGCGCGTGCAGCGGCCTGA

Upstream 100 bases:

>100_bases
GCCTTACCAGCCAGTTTTGTCATGCTGTGCAACAATTCGCGGTCTGGCGGATGGCTGACGAACGCAGCTGGCGCCGGTAC
GGACTCCCGGACTGACACTG

Downstream 100 bases:

>100_bases
GCCCTCAGGCTGCCGCGTTCTTTACCACCACCATCTGCGCGGGCATGGGCGCGGCATAGCCGGCCTGGCCAAAGCTTTCG
CGGATGATGCGGTTGGTGTC

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MPQSSRSQKAARFPAWLLICASLTAIAPLSIDMYLPSFPALAADMRVDIGQIQLTLGTFLVGLALGQAFYGPISDRFGRK
RPLYVGLGLYALAAAGCAMARSAESLMLWRFLQALGGCAGMVMARAVIRDRLDAHESARAFSSMMLVMGLAPILAPIIGG
AILSASGWRAIFWLLTLAGLLILALVHFRMEESLPRERAAPLHPGAIVASYTGLLRDREFLGYSLSAGFGQAGMFAYISG
SPFVLIELNGIDPSHYGFVFGANALGLIGMSQLNARLVHGRTLDSVLRRAVLAPCAAGLILAAAALAGVLVLPVLLAGFF
VFIGSLGCITPNASALALTHQGHRAGTASALMGTLQFSLGTVAGAAVSLWHDGTALPLAVVMAACGMGAVALRAWACAGS
SARAAA

Sequences:

>Translated_406_residues
MPQSSRSQKAARFPAWLLICASLTAIAPLSIDMYLPSFPALAADMRVDIGQIQLTLGTFLVGLALGQAFYGPISDRFGRK
RPLYVGLGLYALAAAGCAMARSAESLMLWRFLQALGGCAGMVMARAVIRDRLDAHESARAFSSMMLVMGLAPILAPIIGG
AILSASGWRAIFWLLTLAGLLILALVHFRMEESLPRERAAPLHPGAIVASYTGLLRDREFLGYSLSAGFGQAGMFAYISG
SPFVLIELNGIDPSHYGFVFGANALGLIGMSQLNARLVHGRTLDSVLRRAVLAPCAAGLILAAAALAGVLVLPVLLAGFF
VFIGSLGCITPNASALALTHQGHRAGTASALMGTLQFSLGTVAGAAVSLWHDGTALPLAVVMAACGMGAVALRAWACAGS
SARAAA
>Mature_405_residues
PQSSRSQKAARFPAWLLICASLTAIAPLSIDMYLPSFPALAADMRVDIGQIQLTLGTFLVGLALGQAFYGPISDRFGRKR
PLYVGLGLYALAAAGCAMARSAESLMLWRFLQALGGCAGMVMARAVIRDRLDAHESARAFSSMMLVMGLAPILAPIIGGA
ILSASGWRAIFWLLTLAGLLILALVHFRMEESLPRERAAPLHPGAIVASYTGLLRDREFLGYSLSAGFGQAGMFAYISGS
PFVLIELNGIDPSHYGFVFGANALGLIGMSQLNARLVHGRTLDSVLRRAVLAPCAAGLILAAAALAGVLVLPVLLAGFFV
FIGSLGCITPNASALALTHQGHRAGTASALMGTLQFSLGTVAGAAVSLWHDGTALPLAVVMAACGMGAVALRAWACAGSS
ARAAA

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=374, Percent_Identity=36.8983957219251, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI48994889, Length=379, Percent_Identity=33.509234828496, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI1790146, Length=249, Percent_Identity=30.1204819277108, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87082312, Length=329, Percent_Identity=28.5714285714286, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787065, Length=363, Percent_Identity=25.8953168044077, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790794, Length=374, Percent_Identity=22.7272727272727, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42139; Mature: 42008

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQSSRSQKAARFPAWLLICASLTAIAPLSIDMYLPSFPALAADMRVDIGQIQLTLGTFL
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHEECHHHHHHHHHHHH
VGLALGQAFYGPISDRFGRKRPLYVGLGLYALAAAGCAMARSAESLMLWRFLQALGGCAG
HHHHHHHHHCCCHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
MVMARAVIRDRLDAHESARAFSSMMLVMGLAPILAPIIGGAILSASGWRAIFWLLTLAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LILALVHFRMEESLPRERAAPLHPGAIVASYTGLLRDREFLGYSLSAGFGQAGMFAYISG
HHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCEECCCCCCCCCEEEEECC
SPFVLIELNGIDPSHYGFVFGANALGLIGMSQLNARLVHGRTLDSVLRRAVLAPCAAGLI
CCEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LAAAALAGVLVLPVLLAGFFVFIGSLGCITPNASALALTHQGHRAGTASALMGTLQFSLG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
TVAGAAVSLWHDGTALPLAVVMAACGMGAVALRAWACAGSSARAAA
HHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PQSSRSQKAARFPAWLLICASLTAIAPLSIDMYLPSFPALAADMRVDIGQIQLTLGTFL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHEECHHHHHHHHHHHH
VGLALGQAFYGPISDRFGRKRPLYVGLGLYALAAAGCAMARSAESLMLWRFLQALGGCAG
HHHHHHHHHCCCHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
MVMARAVIRDRLDAHESARAFSSMMLVMGLAPILAPIIGGAILSASGWRAIFWLLTLAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LILALVHFRMEESLPRERAAPLHPGAIVASYTGLLRDREFLGYSLSAGFGQAGMFAYISG
HHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCEECCCCCCCCCEEEEECC
SPFVLIELNGIDPSHYGFVFGANALGLIGMSQLNARLVHGRTLDSVLRRAVLAPCAAGLI
CCEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LAAAALAGVLVLPVLLAGFFVFIGSLGCITPNASALALTHQGHRAGTASALMGTLQFSLG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
TVAGAAVSLWHDGTALPLAVVMAACGMGAVALRAWACAGSSARAAA
HHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]