Click here to switch to the map view.

The map label for this gene is livM [H]

Identifier: 73537841

GI number: 73537841

Start: 648060

End: 649118

Strand: Direct

Name: livM [H]

Synonym: Reut_B4008

Alternate gene names: 73537841

Gene position: 648060-649118 (Clockwise)

Preceding gene: 73537840

Following gene: 73537842

Centisome position: 23.77

GC content: 67.23

Gene sequence:

>1059_bases
ATGAAGACGCTGATGCGTGCGATGCGGCCCGCCGCATCGTCCGGCGAAGCCGCCGGCGCACCGGCGCCCGCGCGGCGGAA
GTTGCCGGACGGGCGCTGGCATCCGGCCGAATTCTTGTTCTGGCTGTTGCCTGTCGCGGCCTTCTTTGCGCTGCCGGACT
ACCTGATCCTCGGCAGCCAGATCCTGATCAGCGGGCTGTTCGCGCTGTCTCTCGACCTGATCCTTGGCTATGCGGGGATT
GTCTCGCTCGGGCACGCAGCATTCTTCGGACTTGGTGCCTATACGGCTGGCTTGCTGGCGGCGCACGGCTGGGGCGAGCC
GCTCAGTGGCCTGCTGGCCGCCGCGGCAATGGCCGCCGTGGCCGGCTGGCTGTGCAGCTTCCTGGTGGTGCGGGGCGGCG
ATCTCACGCGCCTGATGGTGACGCTGGGCATCGGCCTGATGCTGTTCGAGGCTGCCAACAAGGCGGCATTCGTCACCGGA
GGCGTGGACGGTTTGTCCGGCGTGGCCATGGACCGCCTGCTTGGCGTATTTGAATTCGACTTGATCGGCAAGACCGCCTA
CGTGTACAGCCTGGCGGCGACGTTCGCGCTATTTCTGCTGTTGCGGCGCCTTACCCAGTCGCCGTTCGGCCTGTCGCTGC
GCGGCATACGCGAAGGCGGCCGGCGCATGCCCGCACTCGGTGCTGACGTGCCGCGCCGGTTGCGCACGACGTTCACGCTG
TCCGCAGCGATTGCCGGGGTGGCTGGTGCACTGCTCGCGCAGACGACACAGTTTGTCGGCATGGACGTGCTCGGCTTTCC
GCGCTCGGCGGAGCTGCTCGTCATGCTGGTGCTGGGCGGTACCGGACGCCTGTATGGCGCACTGGCTGGCGCGGCGCTGT
ACATGATTGCGCAGGATGTACTGGCTGGCATGAACCCGGTGTACTGGCAGTTTTGGATCGGCCTGTTGCTGGTGCTGGTG
GTGCTGTTTGCAAGGGGAGGAGTCATGGGCGCCATGGACGCCGCGCGAAGCCGGCTGACGCGGCGTACCGGGACACGGCT
CGAAGGCGGTGCGCCATGA

Upstream 100 bases:

>100_bases
CCGGGAAGTACTACGTGCCAGCAGTCGGTGCCTTTGTCATCTACGCGATGATGGTGCTGTTGCTGGTCCTGTTTCCGGCT
GGCCTGTACGGGAGGCGCGC

Downstream 100 bases:

>100_bases
CAGCAGTACTTCGTACAGAAGGCCTGTCGCGCCAGTGGGGCGCGTTCGTGGCCAACAGCGATATCTCGCTCAACTTCGCG
CCCGGGACACGCCATGCGTT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MKTLMRAMRPAASSGEAAGAPAPARRKLPDGRWHPAEFLFWLLPVAAFFALPDYLILGSQILISGLFALSLDLILGYAGI
VSLGHAAFFGLGAYTAGLLAAHGWGEPLSGLLAAAAMAAVAGWLCSFLVVRGGDLTRLMVTLGIGLMLFEAANKAAFVTG
GVDGLSGVAMDRLLGVFEFDLIGKTAYVYSLAATFALFLLLRRLTQSPFGLSLRGIREGGRRMPALGADVPRRLRTTFTL
SAAIAGVAGALLAQTTQFVGMDVLGFPRSAELLVMLVLGGTGRLYGALAGAALYMIAQDVLAGMNPVYWQFWIGLLLVLV
VLFARGGVMGAMDAARSRLTRRTGTRLEGGAP

Sequences:

>Translated_352_residues
MKTLMRAMRPAASSGEAAGAPAPARRKLPDGRWHPAEFLFWLLPVAAFFALPDYLILGSQILISGLFALSLDLILGYAGI
VSLGHAAFFGLGAYTAGLLAAHGWGEPLSGLLAAAAMAAVAGWLCSFLVVRGGDLTRLMVTLGIGLMLFEAANKAAFVTG
GVDGLSGVAMDRLLGVFEFDLIGKTAYVYSLAATFALFLLLRRLTQSPFGLSLRGIREGGRRMPALGADVPRRLRTTFTL
SAAIAGVAGALLAQTTQFVGMDVLGFPRSAELLVMLVLGGTGRLYGALAGAALYMIAQDVLAGMNPVYWQFWIGLLLVLV
VLFARGGVMGAMDAARSRLTRRTGTRLEGGAP
>Mature_352_residues
MKTLMRAMRPAASSGEAAGAPAPARRKLPDGRWHPAEFLFWLLPVAAFFALPDYLILGSQILISGLFALSLDLILGYAGI
VSLGHAAFFGLGAYTAGLLAAHGWGEPLSGLLAAAAMAAVAGWLCSFLVVRGGDLTRLMVTLGIGLMLFEAANKAAFVTG
GVDGLSGVAMDRLLGVFEFDLIGKTAYVYSLAATFALFLLLRRLTQSPFGLSLRGIREGGRRMPALGADVPRRLRTTFTL
SAAIAGVAGALLAQTTQFVGMDVLGFPRSAELLVMLVLGGTGRLYGALAGAALYMIAQDVLAGMNPVYWQFWIGLLLVLV
VLFARGGVMGAMDAARSRLTRRTGTRLEGGAP

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=312, Percent_Identity=28.8461538461538, Blast_Score=88, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 36923; Mature: 36923

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLMRAMRPAASSGEAAGAPAPARRKLPDGRWHPAEFLFWLLPVAAFFALPDYLILGSQ
CHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ILISGLFALSLDLILGYAGIVSLGHAAFFGLGAYTAGLLAAHGWGEPLSGLLAAAAMAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AGWLCSFLVVRGGDLTRLMVTLGIGLMLFEAANKAAFVTGGVDGLSGVAMDRLLGVFEFD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHH
LIGKTAYVYSLAATFALFLLLRRLTQSPFGLSLRGIREGGRRMPALGADVPRRLRTTFTL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHH
SAAIAGVAGALLAQTTQFVGMDVLGFPRSAELLVMLVLGGTGRLYGALAGAALYMIAQDV
HHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHEEEEECCCCHHHHHHHHHHHHHHHHHH
LAGMNPVYWQFWIGLLLVLVVLFARGGVMGAMDAARSRLTRRTGTRLEGGAP
HHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKTLMRAMRPAASSGEAAGAPAPARRKLPDGRWHPAEFLFWLLPVAAFFALPDYLILGSQ
CHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ILISGLFALSLDLILGYAGIVSLGHAAFFGLGAYTAGLLAAHGWGEPLSGLLAAAAMAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AGWLCSFLVVRGGDLTRLMVTLGIGLMLFEAANKAAFVTGGVDGLSGVAMDRLLGVFEFD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHH
LIGKTAYVYSLAATFALFLLLRRLTQSPFGLSLRGIREGGRRMPALGADVPRRLRTTFTL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHH
SAAIAGVAGALLAQTTQFVGMDVLGFPRSAELLVMLVLGGTGRLYGALAGAALYMIAQDV
HHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHEEEEECCCCHHHHHHHHHHHHHHHHHH
LAGMNPVYWQFWIGLLLVLVVLFARGGVMGAMDAARSRLTRRTGTRLEGGAP
HHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]