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The map label for this gene is flhA [H]

Identifier: 73537833

GI number: 73537833

Start: 638034

End: 639140

Strand: Reverse

Name: flhA [H]

Synonym: Reut_B4000

Alternate gene names: 73537833

Gene position: 639140-638034 (Counterclockwise)

Preceding gene: 73537835

Following gene: 73537832

Centisome position: 23.44

GC content: 64.23

Gene sequence:

>1107_bases
ATGAAAACCAAAGCCGCCATCGCCTGGAAAGCCGGCGCCCCGCTGACCATCGAAGACGTGGACCTGGAAGGCCCCCGTGC
TGGTGAAGTGCTGGTGGAAATCAAGGCCACCGGCATCTGCCATACCGATTACTACACGCTGTCCGGCGCCGATCCTGAAG
GCATCTTCCCGGCGATCCTGGGTCATGAGGGCGCGGGCATCGTCACCGATGTCGGCCCGGGTGTGACCTCGCTCAAGCCG
GGCGACCACGTGATTCCGCTCTACACGCCGGAATGCCGCCAGTGCAAGTTCTGCCTGTCACGCAAGACCAACCTGTGCCA
GGCCATCCGCGCCACGCAGGGCAAGGGCCTGATGCCGGATGGCACCTCACGCTTCTCGATCGACGGCAAGCCGATCTTCC
ACTACATGGGCACATCGACGTTCGCCAACCACATCGTGGTGCCGGAAATCGCGCTCGCGAAGATTCGCCCCGACGCGCCG
TTCGACAAGGTCTGCTACATCGGCTGCGGCGTGACCACCGGCGTGGGCGCCGTCATCTTCACCGCGAAGGTGGAAGCCGG
CGCCAACGTGGTGGTGTTCGGCCTCGGCGGCATTGGCCTGAACGTCATCCAGGGCGCGAAGATGGTCGGCGCGGACAAGA
TCATCGGCGTGGACCTGAACCCCGAGCGCGAAGCGATGGCGCGCAAGTTCGGCATGACGCATTTCATCAACCCGAAGGAC
GTGGGCAACGTCGTCGACCACATCATCCAGCTCACCGACGGCGGCGCGGACTATTCGTTCGAATGCATCGGCAATACGCA
GGTCATGCGCCAGGCGCTCGAGTGCTGCCACAAGGGCTGGGGCAAGTCGATCATCATCGGCGTGGCGGAGGCCGGCGCGG
AGATTTCCACGCGCCCGTTCCAGCTCGTGACCGGTCGCGAATGGAAGGGTTCGGCTTTCGGCGGCGCGCGCGGGCGCACT
GACGTGCCCAAGATCGTCGACTGGTACATGGACGGCAAGCTCAACATCGATGACCTGATCACGCACACGCTGCCGCTGGA
CCGCATCAACGAAGGCTTCGAGCTGATGAGGCGCGGCGAGTCGATCCGCTCGGTGGTGCTGTACTGA

Upstream 100 bases:

>100_bases
CGGCACGCGATAAGTGATGCCTGTATCTCTCCGGAAGGTCGTATAGTCATGCCTCCGGCCCAAGCGGGCCGATCCCTTTC
CTTACCCCCCGGAGGATTCC

Downstream 100 bases:

>100_bases
GGAGCGCGCCGTGACCCTCGAACTGCTGTCCGAACACGGCTGCCATGGCGGCGTGCAGCGCTTCTACCGGCACGCATCGG
CCACCATCGGGCTGCCAATG

Product: zinc-containing alcohol dehydrogenase superfamily protein

Products: NA

Alternate protein names: Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKTKAAIAWKAGAPLTIEDVDLEGPRAGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGIVTDVGPGVTSLKP
GDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPDGTSRFSIDGKPIFHYMGTSTFANHIVVPEIALAKIRPDAP
FDKVCYIGCGVTTGVGAVIFTAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPEREAMARKFGMTHFINPKD
VGNVVDHIIQLTDGGADYSFECIGNTQVMRQALECCHKGWGKSIIIGVAEAGAEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMDGKLNIDDLITHTLPLDRINEGFELMRRGESIRSVVLY

Sequences:

>Translated_368_residues
MKTKAAIAWKAGAPLTIEDVDLEGPRAGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGIVTDVGPGVTSLKP
GDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPDGTSRFSIDGKPIFHYMGTSTFANHIVVPEIALAKIRPDAP
FDKVCYIGCGVTTGVGAVIFTAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPEREAMARKFGMTHFINPKD
VGNVVDHIIQLTDGGADYSFECIGNTQVMRQALECCHKGWGKSIIIGVAEAGAEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMDGKLNIDDLITHTLPLDRINEGFELMRRGESIRSVVLY
>Mature_368_residues
MKTKAAIAWKAGAPLTIEDVDLEGPRAGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGIVTDVGPGVTSLKP
GDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPDGTSRFSIDGKPIFHYMGTSTFANHIVVPEIALAKIRPDAP
FDKVCYIGCGVTTGVGAVIFTAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPEREAMARKFGMTHFINPKD
VGNVVDHIIQLTDGGADYSFECIGNTQVMRQALECCHKGWGKSIIIGVAEAGAEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMDGKLNIDDLITHTLPLDRINEGFELMRRGESIRSVVLY

Specific function: Essential for the oxidation of formaldehyde produced during methylotrophic growth [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=367, Percent_Identity=61.8528610354223, Blast_Score=466, Evalue=1e-131,
Organism=Homo sapiens, GI71565152, Length=374, Percent_Identity=47.8609625668449, Blast_Score=367, Evalue=1e-102,
Organism=Homo sapiens, GI34577061, Length=369, Percent_Identity=46.3414634146341, Blast_Score=358, Evalue=5e-99,
Organism=Homo sapiens, GI4501933, Length=369, Percent_Identity=45.7994579945799, Blast_Score=352, Evalue=4e-97,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=45.9239130434783, Blast_Score=347, Evalue=8e-96,
Organism=Homo sapiens, GI4501929, Length=369, Percent_Identity=45.2574525745258, Blast_Score=345, Evalue=4e-95,
Organism=Homo sapiens, GI262073058, Length=370, Percent_Identity=45.6756756756757, Blast_Score=345, Evalue=4e-95,
Organism=Homo sapiens, GI71743840, Length=370, Percent_Identity=45.6756756756757, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=45.983379501385, Blast_Score=340, Evalue=1e-93,
Organism=Homo sapiens, GI156627571, Length=361, Percent_Identity=25.7617728531856, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=58.5365853658537, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI87082125, Length=382, Percent_Identity=26.7015706806283, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1788073, Length=360, Percent_Identity=27.2222222222222, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1790045, Length=378, Percent_Identity=27.7777777777778, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87081918, Length=344, Percent_Identity=28.4883720930233, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787863, Length=356, Percent_Identity=26.123595505618, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786825, Length=400, Percent_Identity=25, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI226510992, Length=374, Percent_Identity=24.331550802139, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786518, Length=367, Percent_Identity=25.6130790190736, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=55.4959785522788, Blast_Score=438, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=55.2, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17565904, Length=343, Percent_Identity=55.6851311953353, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17562584, Length=366, Percent_Identity=27.3224043715847, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17562876, Length=359, Percent_Identity=28.133704735376, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17562582, Length=355, Percent_Identity=25.0704225352113, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17562878, Length=358, Percent_Identity=26.2569832402235, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71988145, Length=372, Percent_Identity=26.0752688172043, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320033, Length=371, Percent_Identity=54.7169811320755, Blast_Score=438, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6323099, Length=373, Percent_Identity=29.4906166219839, Blast_Score=102, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6319621, Length=370, Percent_Identity=27.027027027027, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6324486, Length=374, Percent_Identity=27.0053475935829, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323729, Length=366, Percent_Identity=27.0491803278689, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323961, Length=365, Percent_Identity=26.027397260274, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6322619, Length=365, Percent_Identity=27.9452054794521, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319955, Length=365, Percent_Identity=27.9452054794521, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6319257, Length=262, Percent_Identity=26.3358778625954, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17737895, Length=368, Percent_Identity=61.1413043478261, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI17137530, Length=358, Percent_Identity=28.4916201117318, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284 [H]

Molecular weight: Translated: 39432; Mature: 39432

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKAAIAWKAGAPLTIEDVDLEGPRAGEVLVEIKATGICHTDYYTLSGADPEGIFPAIL
CCCCEEEEEECCCCEEEEECCCCCCCCCCEEEEEEECEEEECCEEEECCCCCCCCCHHHH
GHEGAGIVTDVGPGVTSLKPGDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPD
CCCCCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
GTSRFSIDGKPIFHYMGTSTFANHIVVPEIALAKIRPDAPFDKVCYIGCGVTTGVGAVIF
CCCEEEECCCEEEEECCCHHHHCCEECCCEEEEEECCCCCCCCEEEEECCCHHCCCEEEE
TAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPEREAMARKFGMTHFINPKD
EEEECCCCCEEEEECCCCCHHHHCCCEEECCCEEEEECCCCHHHHHHHHHCCCCCCCHHH
VGNVVDHIIQLTDGGADYSFECIGNTQVMRQALECCHKGWGKSIIIGVAEAGAEISTRPF
HHHHHHHHHEECCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMDGKLNIDDLITHTLPLDRINEGFELMRRGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCC
SIRSVVLY
HHHHEECC
>Mature Secondary Structure
MKTKAAIAWKAGAPLTIEDVDLEGPRAGEVLVEIKATGICHTDYYTLSGADPEGIFPAIL
CCCCEEEEEECCCCEEEEECCCCCCCCCCEEEEEEECEEEECCEEEECCCCCCCCCHHHH
GHEGAGIVTDVGPGVTSLKPGDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPD
CCCCCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
GTSRFSIDGKPIFHYMGTSTFANHIVVPEIALAKIRPDAPFDKVCYIGCGVTTGVGAVIF
CCCEEEECCCEEEEECCCHHHHCCEECCCEEEEEECCCCCCCCEEEEECCCHHCCCEEEE
TAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPEREAMARKFGMTHFINPKD
EEEECCCCCEEEEECCCCCHHHHCCCEEECCCEEEEECCCCHHHHHHHHHCCCCCCCHHH
VGNVVDHIIQLTDGGADYSFECIGNTQVMRQALECCHKGWGKSIIIGVAEAGAEISTRPF
HHHHHHHHHEECCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMDGKLNIDDLITHTLPLDRINEGFELMRRGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCC
SIRSVVLY
HHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7798140 [H]