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The map label for this gene is yhcK [H]

Identifier: 73537827

GI number: 73537827

Start: 633431

End: 634324

Strand: Reverse

Name: yhcK [H]

Synonym: Reut_B3994

Alternate gene names: 73537827

Gene position: 634324-633431 (Counterclockwise)

Preceding gene: 73537828

Following gene: 73537816

Centisome position: 23.27

GC content: 68.23

Gene sequence:

>894_bases
ATGAGTCCGACAGAATTCACTAATCGCTATGTGCCAACGCTGGAGCCGCTGGAACGACTGCGCAGCGAGTTCCTCAACGA
GGTGATCGCGCATTTTGAGGCGGACCGCCAGCATGCGCTGCGCGTGCTGGTGACGGCTGGCAGCGCGACGGCGCTGATCC
TGCTGGTGCTTGGCTACGTGCTGGTGTTTGCGCAGCGTTCCGTGTTCCTGCCGTTGCTGGATGCGCGCGATGCGGTCATC
GCGCTGGCCGAGGGCCGCAGCCGCCCGAGCTTCACCCTCCCGGCCTCCGGTCGCGAGATGCGGCGCCTGTTCGATGCGCT
GGATATCCTGCGCCGCAAGCTCCGCGAGCGTGCATCGCTCACCGAGCAGCTGGAACACCAGGCCCGCACCGACAGCCTGA
CCGGGCTGCTCAACCGGCGCGCACTGGAACGCATTGCCGCGCAGCCTGCCGATCCCGCCGGACCGTCGACGACGAGCCTG
ATCCTCATGGATGTGGATCGATTCAAGCAGATCAACGACCGGCACGGGCACCCCGCCGGCGATCAGGTACTGCGCGCAGT
GGCTGACCTGATGCGCGATATGGTGGACCCGGACCATGCGCTGGCCCGCTTCGGCGGCGAGGAGTTTGCCGTACTGGTAC
CAGGCGCAAGCGCTGACGAAGCCGCGTCCCTTGCGCAGGCATTATGCGCGGCGATCTGTGCCGAGCCGATCCGCCTGGCC
GATGGCACCCGGCTGCCCGTGACCGCCAGTTTCGGCGTGGCCACCGGACTCAGCGGCGCCCCGCACTGGCCTCGCCTGTT
CGCTGCGGCCGATGCGGCGATGTATCGCGCCAAGGCCGAGGGCCGCAACTGCGTACGCCGCGCGGACGCACCCGCCGAAG
CCGTGGACCACTGA

Upstream 100 bases:

>100_bases
CGCTCGCGCGTCTGCTGGTGCCGCCGCCCACCCCGACAGGTCTGCACCATCACCATCTGCCACCGCTGATGGACAAGGCC
GCCTGTCCGGGCATTACTCG

Downstream 100 bases:

>100_bases
GCCGGCATGCCGTCCGTGGCGGACGGCACCGCTGACGGACTCACAAACCGGACGCACAAACGGAAAAAGGCCCGCATTGC
TGCGGGCCTTTTCTGAATTT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSPTEFTNRYVPTLEPLERLRSEFLNEVIAHFEADRQHALRVLVTAGSATALILLVLGYVLVFAQRSVFLPLLDARDAVI
ALAEGRSRPSFTLPASGREMRRLFDALDILRRKLRERASLTEQLEHQARTDSLTGLLNRRALERIAAQPADPAGPSTTSL
ILMDVDRFKQINDRHGHPAGDQVLRAVADLMRDMVDPDHALARFGGEEFAVLVPGASADEAASLAQALCAAICAEPIRLA
DGTRLPVTASFGVATGLSGAPHWPRLFAAADAAMYRAKAEGRNCVRRADAPAEAVDH

Sequences:

>Translated_297_residues
MSPTEFTNRYVPTLEPLERLRSEFLNEVIAHFEADRQHALRVLVTAGSATALILLVLGYVLVFAQRSVFLPLLDARDAVI
ALAEGRSRPSFTLPASGREMRRLFDALDILRRKLRERASLTEQLEHQARTDSLTGLLNRRALERIAAQPADPAGPSTTSL
ILMDVDRFKQINDRHGHPAGDQVLRAVADLMRDMVDPDHALARFGGEEFAVLVPGASADEAASLAQALCAAICAEPIRLA
DGTRLPVTASFGVATGLSGAPHWPRLFAAADAAMYRAKAEGRNCVRRADAPAEAVDH
>Mature_296_residues
SPTEFTNRYVPTLEPLERLRSEFLNEVIAHFEADRQHALRVLVTAGSATALILLVLGYVLVFAQRSVFLPLLDARDAVIA
LAEGRSRPSFTLPASGREMRRLFDALDILRRKLRERASLTEQLEHQARTDSLTGLLNRRALERIAAQPADPAGPSTTSLI
LMDVDRFKQINDRHGHPAGDQVLRAVADLMRDMVDPDHALARFGGEEFAVLVPGASADEAASLAQALCAAICAEPIRLAD
GTRLPVTASFGVATGLSGAPHWPRLFAAADAAMYRAKAEGRNCVRRADAPAEAVDH

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=175, Percent_Identity=41.1428571428571, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693134, Length=189, Percent_Identity=34.3915343915344, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI87082007, Length=184, Percent_Identity=36.9565217391304, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1787262, Length=177, Percent_Identity=32.2033898305085, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787802, Length=169, Percent_Identity=33.7278106508876, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=33.7579617834395, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787541, Length=201, Percent_Identity=31.8407960199005, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788381, Length=189, Percent_Identity=32.2751322751323, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786584, Length=171, Percent_Identity=30.4093567251462, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787056, Length=161, Percent_Identity=33.5403726708075, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788956, Length=186, Percent_Identity=31.1827956989247, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788085, Length=199, Percent_Identity=27.1356783919598, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI87081977, Length=178, Percent_Identity=32.0224719101124, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR011620 [H]

Pfam domain/function: PF07694 5TM-5TMR_LYT; PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 32299; Mature: 32168

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTEFTNRYVPTLEPLERLRSEFLNEVIAHFEADRQHALRVLVTAGSATALILLVLGYV
CCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LVFAQRSVFLPLLDARDAVIALAEGRSRPSFTLPASGREMRRLFDALDILRRKLRERASL
HHHHHHHHHHHHHCCCCHHEEEECCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
TEQLEHQARTDSLTGLLNRRALERIAAQPADPAGPSTTSLILMDVDRFKQINDRHGHPAG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCH
DQVLRAVADLMRDMVDPDHALARFGGEEFAVLVPGASADEAASLAQALCAAICAEPIRLA
HHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCHHHHC
DGTRLPVTASFGVATGLSGAPHWPRLFAAADAAMYRAKAEGRNCVRRADAPAEAVDH
CCCCCCEEECCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure 
SPTEFTNRYVPTLEPLERLRSEFLNEVIAHFEADRQHALRVLVTAGSATALILLVLGYV
CCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LVFAQRSVFLPLLDARDAVIALAEGRSRPSFTLPASGREMRRLFDALDILRRKLRERASL
HHHHHHHHHHHHHCCCCHHEEEECCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
TEQLEHQARTDSLTGLLNRRALERIAAQPADPAGPSTTSLILMDVDRFKQINDRHGHPAG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCH
DQVLRAVADLMRDMVDPDHALARFGGEEFAVLVPGASADEAASLAQALCAAICAEPIRLA
HHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCHHHHC
DGTRLPVTASFGVATGLSGAPHWPRLFAAADAAMYRAKAEGRNCVRRADAPAEAVDH
CCCCCCEEECCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]