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The map label for this gene is garR [C]

Identifier: 73537778

GI number: 73537778

Start: 577681

End: 578556

Strand: Reverse

Name: garR [C]

Synonym: Reut_B3944

Alternate gene names: 73537778

Gene position: 578556-577681 (Counterclockwise)

Preceding gene: 73537779

Following gene: 73537777

Centisome position: 21.22

GC content: 68.04

Gene sequence:

>876_bases
ATGCGTGTCGCATTCCTCGGGCTTGGCGTCATGGGTTTCCACATGGCCGGCCACCTGGCCGCCAAGGGCCATGAAGTCAC
TGTCTACAACCGCACTGCAGCGAAGGCGCAGGCCTGGGTCGAGAAGTTTGGCGGCAAGGCGGCCGACACCCCGGCGCAGG
CCGTCAGGCAGGCCGACGTAGTCTGCTCGTGCGTGGGCAACGACGACGACCTGCGCTCGGTGTTGACTGGTCCGGATGGC
GCGTTCTTCGCCGCGCCCACGGGCTGCATCTTCGTCGACCACACCACCGCCAGCGCCAACGTGGCGCGCGAACTCTACGC
GGCGGCGCGCGAGCGCGGCCTGCATTTCGTCGACGGCCCCGTGTCCGGCGGCGAAGTCGGCGCGGAGAAAGGCATCCTGA
CCATCATGTGCGGCGGCGACGCCGACGTCTACGCGCGCGTCGAGCCCGTGATCGCCGCGTATGCGCGCGCCGTGACGCGC
ATTGGCGAAGCGGGCGCAGGCCAGCTCGCGAAGATGGTCAACCAGATCAGCATCGCCGGCCTGATCCAGGGCTTGTCCGA
AGCCATCGCGTTCGGCGAGCGCGCCGGCCTCGACATGAAGCTGGTGCTCGACGTCATCAGCAAGGGCGCGGCCGGTTCCT
GGCAGCTCGAGAATCGCGGCCCGACCATGATCGAAAACAAGTTCGACTTCGGCTTCGCCGTGGACTGGATGCGCAAGGAC
CTGGGCCTGTGCCTGGACGAGGCGCGCCGCAACGGCGCGAGCCTGCCGGTCACCGCGACGGTCGACCAGTTCTATGCCGA
CCTGCAGCAGATGGGCTGCGGCCGCTCGGACACATCGTCGCTGATCAAGCGCCTGCGCCGGTTTTCGCAGAACTGA

Upstream 100 bases:

>100_bases
CCCCGCGCTGCGCGCTTCTGTTGCGACCGCAGGATGCGCTACATGAGCCGCCGCGCGCCACCGACATCAAACAACATCAA
ATCAGTTACGGAGTCGCAGC

Downstream 100 bases:

>100_bases
CTGAGCGATCCGGACGGCACCTGGCCGTCCGACAAGTCCTACATGGCAATCGGCCTGCGCCCGAAAGGCTCGATGTATCC
GCGTGCCTAAGCTGGAAGGC

Product: 6-phosphogluconate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MRVAFLGLGVMGFHMAGHLAAKGHEVTVYNRTAAKAQAWVEKFGGKAADTPAQAVRQADVVCSCVGNDDDLRSVLTGPDG
AFFAAPTGCIFVDHTTASANVARELYAAARERGLHFVDGPVSGGEVGAEKGILTIMCGGDADVYARVEPVIAAYARAVTR
IGEAGAGQLAKMVNQISIAGLIQGLSEAIAFGERAGLDMKLVLDVISKGAAGSWQLENRGPTMIENKFDFGFAVDWMRKD
LGLCLDEARRNGASLPVTATVDQFYADLQQMGCGRSDTSSLIKRLRRFSQN

Sequences:

>Translated_291_residues
MRVAFLGLGVMGFHMAGHLAAKGHEVTVYNRTAAKAQAWVEKFGGKAADTPAQAVRQADVVCSCVGNDDDLRSVLTGPDG
AFFAAPTGCIFVDHTTASANVARELYAAARERGLHFVDGPVSGGEVGAEKGILTIMCGGDADVYARVEPVIAAYARAVTR
IGEAGAGQLAKMVNQISIAGLIQGLSEAIAFGERAGLDMKLVLDVISKGAAGSWQLENRGPTMIENKFDFGFAVDWMRKD
LGLCLDEARRNGASLPVTATVDQFYADLQQMGCGRSDTSSLIKRLRRFSQN
>Mature_291_residues
MRVAFLGLGVMGFHMAGHLAAKGHEVTVYNRTAAKAQAWVEKFGGKAADTPAQAVRQADVVCSCVGNDDDLRSVLTGPDG
AFFAAPTGCIFVDHTTASANVARELYAAARERGLHFVDGPVSGGEVGAEKGILTIMCGGDADVYARVEPVIAAYARAVTR
IGEAGAGQLAKMVNQISIAGLIQGLSEAIAFGERAGLDMKLVLDVISKGAAGSWQLENRGPTMIENKFDFGFAVDWMRKD
LGLCLDEARRNGASLPVTATVDQFYADLQQMGCGRSDTSSLIKRLRRFSQN

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=283, Percent_Identity=26.1484098939929, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI23308751, Length=292, Percent_Identity=28.0821917808219, Blast_Score=122, Evalue=3e-28,
Organism=Escherichia coli, GI145693186, Length=281, Percent_Identity=34.1637010676157, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI1786719, Length=291, Percent_Identity=33.3333333333333, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1790315, Length=287, Percent_Identity=29.9651567944251, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1789092, Length=238, Percent_Identity=33.6134453781513, Blast_Score=105, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17557316, Length=269, Percent_Identity=27.5092936802974, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24655230, Length=296, Percent_Identity=28.0405405405405, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19922568, Length=296, Percent_Identity=28.0405405405405, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI28574115, Length=283, Percent_Identity=25.4416961130742, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30804; Mature: 30804

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVAFLGLGVMGFHMAGHLAAKGHEVTVYNRTAAKAQAWVEKFGGKAADTPAQAVRQADV
CEEEEEEHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
VCSCVGNDDDLRSVLTGPDGAFFAAPTGCIFVDHTTASANVARELYAAARERGLHFVDGP
HEEECCCCHHHHHHHCCCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEECCC
VSGGEVGAEKGILTIMCGGDADVYARVEPVIAAYARAVTRIGEAGAGQLAKMVNQISIAG
CCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LIQGLSEAIAFGERAGLDMKLVLDVISKGAAGSWQLENRGPTMIENKFDFGFAVDWMRKD
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCCCCEECCCCCCCHHHHHHHHH
LGLCLDEARRNGASLPVTATVDQFYADLQQMGCGRSDTSSLIKRLRRFSQN
HHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVAFLGLGVMGFHMAGHLAAKGHEVTVYNRTAAKAQAWVEKFGGKAADTPAQAVRQADV
CEEEEEEHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
VCSCVGNDDDLRSVLTGPDGAFFAAPTGCIFVDHTTASANVARELYAAARERGLHFVDGP
HEEECCCCHHHHHHHCCCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEECCC
VSGGEVGAEKGILTIMCGGDADVYARVEPVIAAYARAVTRIGEAGAGQLAKMVNQISIAG
CCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LIQGLSEAIAFGERAGLDMKLVLDVISKGAAGSWQLENRGPTMIENKFDFGFAVDWMRKD
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCCCCEECCCCCCCHHHHHHHHH
LGLCLDEARRNGASLPVTATVDQFYADLQQMGCGRSDTSSLIKRLRRFSQN
HHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA